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Offlineblueyz
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Registered: 11/09/13
Posts: 50
Loc: CO
Last seen: 9 years, 5 months
Newb Question
    #19244837 - 12/08/13 12:49 PM (10 years, 1 month ago)

I have a buddy who inoculated some jars according to the PF tek (shitokai of course), but had no growth, good or bad. The only deviation was he sterilized the jars and left them in the PC for almost a week because there was not an ideal time to inoculate. Is that reason enough to stunt the growth? Do you think the substrate dried out too much?

Thanks


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Peace & Love


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OfflinecronicrFacebook
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Registered: 08/07/11
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Re: Newb Question [Re: blueyz]
    #19245011 - 12/08/13 01:26 PM (10 years, 1 month ago)

no it wouldn't have dried out too much


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It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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OfflineTheMustardTiger
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Registered: 08/19/13
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Re: Newb Question [Re: cronicr]
    #19245233 - 12/08/13 02:12 PM (10 years, 1 month ago)

Was this a MS inoculation? Sounds like contam or just piss poor spores. Give them a smell?  Try to to get a cake out (Outside of your grow area, of course) and give it a good inspection for contams or anything else that May have halted or prevented any growth.


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OfflineHalophorm
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Re: Newb Question [Re: TheMustardTiger]
    #19245266 - 12/08/13 02:20 PM (10 years, 1 month ago)

Quote:

MustardTiger66 said:
Was this a MS inoculation? Sounds like contam or just piss poor spores. Give them a smell?  Try to to get a cake out (Outside of your grow area, of course) and give it a good inspection for contams or anything else that May have halted or prevented any growth.



:cookiemonster: what he said :thumbup:


--------------------
"Imagination is more important than knowledge." -Albert Einstien


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OfflineHalophorm
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Re: Newb Question [Re: blueyz]
    #19245279 - 12/08/13 02:23 PM (10 years, 1 month ago)

OP can we get a little more info? how long has it been since you inoculated? how did you inoculate? and also pics would help if possible.


--------------------
"Imagination is more important than knowledge." -Albert Einstien


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OfflineTheMustardTiger
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Re: Newb Question [Re: Halophorm]
    #19245286 - 12/08/13 02:24 PM (10 years, 1 month ago)

Quote:

Halophorm said:
OP can we get a little more info? how long has it been since you inoculated? how did you inoculate? and also pics would help if possible.



:thumbup:


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OfflinePussyFart
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Re: Newb Question [Re: TheMustardTiger]
    #19245303 - 12/08/13 02:29 PM (10 years, 1 month ago)

Quote:

blueyz said:
had no growth, good or bad.



Quote:

MustardTiger66 said:
Sounds like contam



:facepalm:    :lmafo:


Edited by PussyFart (12/08/13 02:29 PM)


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OfflineTheMustardTiger
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Registered: 08/19/13
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Re: Newb Question [Re: PussyFart]
    #19245330 - 12/08/13 02:35 PM (10 years, 1 month ago)

Quote:

Notahacker420 said:
Quote:

blueyz said:
had no growth, good or bad.



Quote:

MustardTiger66 said:
Sounds like contam



:facepalm:    :lmafo:




I've had contams in the center of cakes, not visible while still housed in a jar. It's a good idea to inspect/smell one regardless to rule out contam so you can move on to another diagnosis. Keep the face palm to yourself.


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OfflinePussyFart
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Re: Newb Question [Re: Halophorm]
    #19245341 - 12/08/13 02:37 PM (10 years, 1 month ago)

Quote:

Halophorm said:
OP can we get a little more info? how long has it been since you inoculated? how did you inoculate? and also pics would help if possible.




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Offlineblueyz
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Re: Newb Question [Re: TheMustardTiger]
    #19249048 - 12/09/13 07:45 AM (10 years, 1 month ago)

Quote:

MustardTiger66 said:
Was this a MS inoculation? Sounds like contam or just piss poor spores. Give them a smell?  Try to to get a cake out (Outside of your grow area, of course) and give it a good inspection for contams or anything else that May have halted or prevented any growth.




Ok- Thanks for the advice. I'm not sure what I am smelling for being this is my first attempted grow. I'm not sure if I am answering your "MS inoculation" question correctly, but the first syringe was GT. I got nothing out of that and they sat for about a month. The second go around was also GT but from another supplier. This time I could physically see a ton of spores. I couldn't see any spores with the first syringe. I also have them in a thermostat controlled incubator, set to approximately 80F because without it they would be around 65F. It looks like I definitely have some growth on 1 of the jars, but I can't tell with any of the others. Should I isolate the 1 jar that has growth? Thx!



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Peace & Love


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Offlineblueyz
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Registered: 11/09/13
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Re: Newb Question [Re: PussyFart]
    #19249060 - 12/09/13 07:50 AM (10 years, 1 month ago)

Quote:

Notahacker420 said:
Quote:

Halophorm said:
OP can we get a little more info? how long has it been since you inoculated? how did you inoculate? and also pics would help if possible.







First inoculation was about a month ago. I got nothing. I inoculated a second go around (new substrate) about 4 days ago. I see definitive growth on only 1 of the jars.


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OfflineImFukNCLUELESS
I SPIT ON PEOPLE
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Registered: 11/19/12
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Last seen: 9 years, 19 days
Re: Newb Question [Re: blueyz]
    #19249206 - 12/09/13 09:06 AM (10 years, 1 month ago)

Give it more time it has took me over 2 weeks before seeing any growth 4 days aint shit

also 80 degrees is to hot (risk of contams) bring the temp down to low-mid 70's with indirect light
12/12 schedule and take the foil off the top


--------------------
how to pass a drug test
FOO MAN'S WBS TEK
damions5050's coir tek
DONATE TO THE  "IM FUCKING BROKE FUND" 1PtqhURaxtCpGpeUBNqeZi7XnmKwWe8WWf

"my girl said it's OK to have a little penis
I prefer she didnt have a penis at all"-prisoner#1


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OfflineTheMustardTiger
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Re: Newb Question [Re: ImFukNCLUELESS]
    #19249336 - 12/09/13 09:54 AM (10 years, 1 month ago)

Quote:

1slow2v said:
Give it more time it has took me over 2 weeks before seeing any growth 4 days aint shit

also 80 degrees is to hot (risk of contams) bring the temp down to low-mid 70's with indirect light
12/12 schedule and take the foil off the top



:cookiemonster:


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Offlineblueyz
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Registered: 11/09/13
Posts: 50
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Re: Newb Question [Re: ImFukNCLUELESS]
    #19249364 - 12/09/13 10:04 AM (10 years, 1 month ago)

Quote:

1slow2v said:
Give it more time it has took me over 2 weeks before seeing any growth 4 days aint shit

also 80 degrees is to hot (risk of contams) bring the temp down to low-mid 70's with indirect light
12/12 schedule and take the foil off the top




Great- will do thanks. What is the purpose of taking the foil off the top though? Wouldn't that increase the risk of contams? Shouldn't their be enough room for gas exchange even with it on?


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OfflineImFukNCLUELESS
I SPIT ON PEOPLE
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Registered: 11/19/12
Posts: 1,580
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Re: Newb Question [Re: blueyz]
    #19249409 - 12/09/13 10:20 AM (10 years, 1 month ago)

No it henders GE u have a dry verm layer on top dont u that is all u need the foil could be the cause for such slow growth


--------------------
how to pass a drug test
FOO MAN'S WBS TEK
damions5050's coir tek
DONATE TO THE  "IM FUCKING BROKE FUND" 1PtqhURaxtCpGpeUBNqeZi7XnmKwWe8WWf

"my girl said it's OK to have a little penis
I prefer she didnt have a penis at all"-prisoner#1


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OfflineTheMustardTiger
Male

Registered: 08/19/13
Posts: 4,700
Loc: Sunnyvale Trailer Park
Last seen: 6 years, 5 months
Re: Newb Question [Re: ImFukNCLUELESS]
    #19249571 - 12/09/13 11:04 AM (10 years, 1 month ago)

Quote:

1slow2v said:
No it henders GE u have a dry verm layer on top dont u that is all u need the foil could be the cause for such slow growth



:thumbup: I made this mistake on my first grow. Take that shit off. It's only purpose is to protect the top of your jar during PC.


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OfflineTheWiz
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Registered: 11/21/11
Posts: 191
Loc: Southern IL
Last seen: 3 years, 4 months
Re: Newb Question [Re: TheMustardTiger]
    #19249864 - 12/09/13 12:20 PM (10 years, 1 month ago)

Quote:

MustardTiger66 said:
Quote:

Notahacker420 said:
Quote:

blueyz said:
had no growth, good or bad.



Quote:

MustardTiger66 said:
Sounds like contam



:facepalm:    :lmafo:




I've had contams in the center of cakes, not visible while still housed in a jar. It's a good idea to inspect/smell one regardless to rule out contam so you can move on to another diagnosis. Keep the face palm to yourself.



Also, bacterial contams aren't always obvious and can go unnoticed by beginners.


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I'd hit it.


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Offlineblueyz
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Registered: 11/09/13
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Re: Newb Question [Re: ImFukNCLUELESS]
    #19249994 - 12/09/13 12:52 PM (10 years, 1 month ago)

Quote:

1slow2v said:
No it henders GE u have a dry verm layer on top dont u that is all u need the foil could be the cause for such slow growth




Ahhhh, got it. Makes sense. Thanks!


--------------------
Peace & Love


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OfflinePussyFart
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Registered: 04/08/12
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Re: Newb Question [Re: ImFukNCLUELESS]
    #19250070 - 12/09/13 01:09 PM (10 years, 1 month ago)

Quote:

1slow2v said:
No it henders GE u have a dry verm layer on top dont u that is all u need the foil could be the cause for such slow growth



I doubt it.....the foil is not sealing it air tight.

Gasses will pass thru the path of least resistance.


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OfflineHalophorm
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Re: Newb Question [Re: ImFukNCLUELESS]
    #19258219 - 12/10/13 11:34 PM (10 years, 1 month ago)

Quote:

1slow2v said:
Give it more time it has took me over 2 weeks before seeing any growth 4 days aint shit

also 80 degrees is to hot (risk of contams) bring the temp down to low-mid 70's with indirect light
12/12 schedule and take the foil off the top




CORRECT!  :thumbup:


--------------------
"Imagination is more important than knowledge." -Albert Einstien


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