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OfflineLizardCZ
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Registered: 12/08/13
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Last seen: 8 years, 8 months
Syringe spore to LC (time)
    #19244424 - 12/08/13 10:54 AM (10 years, 2 months ago)

Hello guys, and thanks for your answers.
The 27/11 I made 2 LC jars. I used 250ml distilled water and 4% Maple syrup. Then i injected 1 ml for each with PS B+ spore syringe.
Now they are in my cabinet, in the dark.
At the moment i never seen mycelium development. Why?
I shook my syringe before use but now in 8ml remain, I still can see the largest "mycelium cloud".
So, now? What should I do? Make a new one jar?

Thanks, sorry for my bad language and regards from Italy. :grin:


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OfflinePussyFart
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Re: Syringe spore to LC (time) [Re: LizardCZ]
    #19244450 - 12/08/13 11:01 AM (10 years, 2 months ago)

Just use the spores on whatever you were going to use the LC on.....in other words forget about making a LC.

LCs are a big risky waste of time in my opinion....if you skip it you will be miles ahead.


Edited by PussyFart (12/08/13 11:01 AM)


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OfflineLizardCZ
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Re: Syringe spore to LC (time) [Re: PussyFart]
    #19244493 - 12/08/13 11:09 AM (10 years, 2 months ago)

Yeah, I still used 2ml of spores on cakes, but i need to clones my mycelium with LC. I cant buy forever a new one. I want to have LC.
Why do you say this? It's my first time that i hear this!


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InvisiblePastywhyteMDiscord
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Re: Syringe spore to LC (time) [Re: LizardCZ]
    #19244520 - 12/08/13 11:20 AM (10 years, 2 months ago)

LC is notorious for contams, especially with clones or ms solution. You cannot tell if it is clean visually and most tissue clones will have some contamination. Spore syringes are almost never sterile either so you take a real risk using them for LC. If you want to clone use agar. The only LC that I would use is one that was inoculated with an agar wedge.


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OfflinePussyFart
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Re: Syringe spore to LC (time) [Re: LizardCZ]
    #19244531 - 12/08/13 11:23 AM (10 years, 2 months ago)

Quote:

LizardCZ said:
i need to clones my mycelium with LC.



This makes no sense....you do not clone with spores and LC.

Quote:

LizardCZ said:
I cant buy forever a new one. I want to have LC.



There is no need to keep buying spores.....you only need a few drops per jar to get growth, this means that a 10ml syringe can knock up ~100 jars if done properly...

You can then clone one of your fruits on agar and keep that culture alive literally forever...no need for spores ever again...unless you want to try another variety.

Do you want to be successful?

Or do you just want LC?

Because if you just want LC then by all means have at it, but that is not the best option....it is by far the riskiest option.

Quote:

LizardCZ said:
Why do you say this?



Because i want you to be successful and not a statistic.


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OfflineLizardCZ
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Re: Syringe spore to LC (time) [Re: PussyFart]
    #19244580 - 12/08/13 11:34 AM (10 years, 2 months ago)

Mhmmm lol im confused now lol.
Maybe I was wrong to write "clone" :P I want to have always a liquid syringe :P

I always read, to make a LC, just to have always a "hungry and strong" mycelium.
Unfortunately I cant do now clones with Agar and Petri (also cause it's hard for me generate a spore print). I have my GloveBox and i can do only Jars. So, just to start i want to have my Master LC jar :P :P
I always see infect with it the cakes. So that's why I want to obtain it. It's too easy for me to use LC.
Thanks and regards men.


Edited by LizardCZ (12/08/13 11:36 AM)


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OfflinePussyFart
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Re: Syringe spore to LC (time) [Re: LizardCZ]
    #19244595 - 12/08/13 11:40 AM (10 years, 2 months ago)

Quote:

LizardCZ said:
Mhmmm lol im confused now lol.
Maybe I was wrong to write "clone" :P I want to have always a liquid syringe :P



Ok, so take spore prints from your mushrooms and make more spore syringes....LCs are too risky to always depend on.

Quote:

LizardCZ said:
I always read, to make a LC, just to have always a "hungry and strong" mycelium.



There are better, more efficient ways.

Quote:

LizardCZ said:
I have my GloveBox and i can do only Jars. So, just to start i want to have my Master LC jar :P :P



Ok, but you are setting yourself up for failure, considering you can't even get cake inoculations down successfully.

Quote:

LizardCZ said:
I always see infect with it the cakes. So that's why I want to obtain it. It's too easy for me to use LC.



It's too easy for the LC to contaminate....if you always see infections on cakes why would LC be any different?

It is obviously your sterile technique that needs work, or you have a bad syringe.


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Offlineblueconfusion
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Re: Syringe spore to LC (time) [Re: LizardCZ]
    #19244608 - 12/08/13 11:44 AM (10 years, 2 months ago)

Seriously DO NOT make an LC it is so attractive this I know I made a few when i was still new, but really you will just end up with a huge failure.  Learn to do grain to grain transfers if you can't do agar.  I know what seems daunting I was scared and didn't know where to get all the supplies and it seems so expensive, but trust myself and others when we tell you agar is the way to go not LC!!!


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OfflineLizardCZ
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Re: Syringe spore to LC (time) [Re: blueconfusion]
    #19244634 - 12/08/13 11:55 AM (10 years, 2 months ago)

OK i understand, here LC is not good accepted ahahaha xD
If i good underst. , LCs are more easy contaminable right? But if I operate in sterile conditions, and without making any mistakes?

Can you tell me the better way to follow to have always a fresh syringe in your opinion? :smile:


PS: Do i clean my actual LC or can i try to grow it? Is it normal? I inoculate 27/11 and till today i never saw nothing.

thanks!!! :smile:


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Offlineblueconfusion
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Re: Syringe spore to LC (time) [Re: LizardCZ]
    #19244666 - 12/08/13 12:04 PM (10 years, 2 months ago)

Make spore prints to always have a good syringe.


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OfflinePussyFart
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Re: Syringe spore to LC (time) [Re: LizardCZ]
    #19244678 - 12/08/13 12:08 PM (10 years, 2 months ago)

Quote:

LizardCZ said:
OK i understand, here LC is not good accepted ahahaha xD
If i good underst. , LCs are more easy contaminable right? But if I operate in sterile conditions, and without making any mistakes?



You cannot control the fact that the syringe you are using is not 100% clean....the cap from which the spores came was most likely fruited in open air.

LCs are the perfect breeding ground for everything nasty....meaning you want to inoculate it with a clean agar wedge and that is it...not spores that are most likely a little dirty.

Quote:

LizardCZ said:
Can you tell me the better way to follow to have always a fresh syringe in your opinion? :smile:



Take a spore print from one of your fruits, and make several syringes....

http://www.mushroomvideos.com/Sporeprinting-Syringes

Quote:

LizardCZ said:
PS: Do i clean my actual LC or can i try to grow it? Is it normal? I inoculate 27/11 and till today i never saw nothing.



There is no way to clean a LC...if it is contaminated the whole thing gets tossed.

YOu need to shoot up test jars to find out if it is good or not....we cannot tell you.


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Offlineblueconfusion
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Re: Syringe spore to LC (time) [Re: PussyFart]
    #19244735 - 12/08/13 12:23 PM (10 years, 2 months ago)

:whathesaid: he knows wtf he's talking about


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OfflineLizardCZ
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Re: Syringe spore to LC (time) [Re: blueconfusion]
    #19244775 - 12/08/13 12:34 PM (10 years, 2 months ago)

LoL I just wanna say if it's better to grow my actual LC or destroy it :P


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Offlinekrypto2000
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Re: Syringe spore to LC (time) [Re: LizardCZ]
    #19244856 - 12/08/13 12:53 PM (10 years, 2 months ago)

If there is nothing growing in your LC then get rid of it, it's useless at this point and you should never reinoculate any medium whether it's grain, pf-cakes, or LC. If you see growth in your LC then inoculate a test jar or two to see if it's clean. If that/those jars are good then you have a clean LC and can proceed to inoculate more jars. Otherwise use your MS syringe to make more jars, do g2g transfers to expand, or put some on agar if you want to go that route.


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InvisibleFilthyShroomz
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Re: Syringe spore to LC (time) [Re: PussyFart]
    #20700223 - 10/14/14 03:22 AM (9 years, 4 months ago)

Isn't making spore syringes with spore prints risky as well in terms of contamination?


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No statements made in any post or message by myself should be construed to mean that I am now, or have ever been, participating in or considering participation in any activities in violation of any local, state, or federal laws. All posts are works of fiction.


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InvisiblebodhisattaMDiscordReddit
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Re: Syringe spore to LC (time) [Re: FilthyShroomz]
    #20700323 - 10/14/14 05:08 AM (9 years, 4 months ago)

not as risky since spore syringes are made with distilled water and don't colonize. you can't separate the contamination in a LC but with a spore syringe you can still inoculate agar and save yourself.

http://www.shroomery.org/forums/showflat.php/Number/20656970


Edited by Trusted cuItivator (10/14/14 05:08 AM)


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Offlinemigraineur
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Re: Syringe spore to LC (time) [Re: bodhisatta]
    #20700786 - 10/14/14 09:07 AM (9 years, 4 months ago)

I know liquid cultures get a bad reputation but I used them successfully for years. I made them from spore prints and spore syringes.

If you're a bit of a novice and your ability to obtain more spore prints and syringes is limited then making a liquid culture is a good way to stretch your supply. One drop from a spore syringe and you can make HEAPS of liquid culture.

I understand that there's a risk of contamination but if you have good sterile technique and if your liquid culture is successful then you're in a very good position. You end up with more liquid culture than you know what to do with.


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Offlineblueconfusion
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Re: Syringe spore to LC (time) [Re: migraineur]
    #20701136 - 10/14/14 11:11 AM (9 years, 4 months ago)

Agar and sterile technique to lc you mean


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InvisibleFilthyShroomz
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Re: Syringe spore to LC (time) [Re: blueconfusion]
    #20702158 - 10/14/14 02:47 PM (9 years, 4 months ago)

Can yollanyone hook it up With an agar tek


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No statements made in any post or message by myself should be construed to mean that I am now, or have ever been, participating in or considering participation in any activities in violation of any local, state, or federal laws. All posts are works of fiction.


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Offlineblueconfusion
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Re: Syringe spore to LC (time) [Re: FilthyShroomz]
    #20702339 - 10/14/14 03:25 PM (9 years, 4 months ago)

Pasty plates or my agar tek work


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