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Psilosopherr
A psilly goose



Registered: 02/15/12
Posts: 12,278
Last seen: 1 month, 10 days
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Does anybody else use small jars as petri dishes?
#19242226 - 12/07/13 07:05 PM (10 years, 1 month ago) |
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Since glass petri dishes are spendy, and I ran out of plastic ones I started using tiny jars.
I've been PC'ing my agar mix in a bottle and pouring it into the sterilized 'petri jars' under a glove box.
But couldn't I just pour it in the jars and then cook it? or would that cause splatter?
Any other tips for using petri jars?
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nksfo5
traverse traveler


Registered: 12/19/12
Posts: 689
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Re: Does anybody else use small jars as petri dishes? [Re: Psilosopherr]
#19242235 - 12/07/13 07:08 PM (10 years, 1 month ago) |
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bodhisatta 
Smurf real estate agent



Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Does anybody else use small jars as petri dishes? [Re: Psilosopherr]
#19242239 - 12/07/13 07:10 PM (10 years, 1 month ago) |
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a "no pour" tek will allow you to cook agar in the PC inside your "petri dish"
Like this one somewhat clever
There's a bunch of other no pour teks out there this is just one example they're all pretty easy to find if you search "no pour agar tek"
I've used all sorts of different small pp5 containers to do agar in but I find myself still pouring dishes, (i have 48 glass dishes given to me free)
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invitro


Registered: 05/03/13
Posts: 2,529
Last seen: 1 month, 20 days
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Re: Does anybody else use small jars as petri dishes? [Re: Psilosopherr]
#19242277 - 12/07/13 07:21 PM (10 years, 1 month ago) |
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I just started experimenting with this, using a lid with an rtv silicone port. One thing I noted is a lot of condensation after pcing, though I'm not sure why. There is so much condensation that the whole surface of the jelly is covered in a thin layer of water.
I used lme, agar and a small amount of dextrose. I cut the agar in half and it still jelled fine. I found out that there is a strong vacuum inside the jar after pcing so adding a whatman filter to the lid might have been a good idea? I should read some good teks to get this procedure down better but I can't understand why I had so much water on top of the jelly when I poured the liquid into the jar when it was cooling and just about to solidify, maybe I needed more agar to trap the water?
The vacuum was caused by me putting the rubber ring side of the lid down by the way, lesson learned.
Edited by invitro (12/07/13 07:23 PM)
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HypnotoadCroaked
Retired, but will check MSGs

Registered: 01/05/13
Posts: 1,168
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Re: Does anybody else use small jars as petri dishes? [Re: invitro]
#19242304 - 12/07/13 07:30 PM (10 years, 1 month ago) |
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http://www.shroomery.org/forums/showflat.php/Number/19223885
There is lots of great info on this thread involving small containers as agar, condensation issues and even a few ideas about gas-exchange ports. There is also plenty of the standard OMC rhetoric. Any of us should be able to get the best out of what that thread is offering, while leaving the bad behind.
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Psilosopherr
A psilly goose



Registered: 02/15/12
Posts: 12,278
Last seen: 1 month, 10 days
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Re: Does anybody else use small jars as petri dishes? [Re: HypnotoadCroaked]
#19242343 - 12/07/13 07:44 PM (10 years, 1 month ago) |
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wow, thanks for all the help guys! doing some reading now.
I can't find PP5 containers to buy by searching PP5 jars..
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HypnotoadCroaked
Retired, but will check MSGs

Registered: 01/05/13
Posts: 1,168
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Re: Does anybody else use small jars as petri dishes? [Re: Psilosopherr]
#19242358 - 12/07/13 07:48 PM (10 years, 1 month ago) |
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Quote:
rbalzer said:
I can't find PP5 containers to buy by searching PP5 jars..
Go to the grocery store, and look for name brand "glad" And "Ziplock"
(On the bottom of the jars it will have a #5 in a recycling triangle).

I prefer the 2 cup screw lids, and of the 2 brands I prefer the ziplock ONLY because the Glad have "deeper edges" than the center, while the ziplock brands have a flat bottom. This makes for slightly less agar per jar, AND if you are like me and grow cultures out to the edge (Im lazy), the edges wont be thick chunks. Does it make a difference? Probably not. ANYTHING with the pp5 logo will withstand the heat of the Pressure cooker. There is a world of various containers that are PP5. Find one you love, and own it!
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DeadPhan



Registered: 05/05/04
Posts: 5,260
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Re: Does anybody else use small jars as petri dishes? [Re: Psilosopherr]
#19242417 - 12/07/13 08:07 PM (10 years, 1 month ago) |
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i do. i use short half pint mason jars. i dont alter the lids. just have the rubber side facing up. i actually hot glued them to the rings so its just easier during inoculation. the hotglue withstands a few pressure cooks and then maybe you have to reapply.
i follow doc_t's no pour agar tek, as he has measurements for when only using about 15 jars. imo you can turn that recipe into alot more than 15 jars as when i do it i proly use about close to double that with a decent depth. maybe around 25 easily. im always doing different but usualy at least 15 at a time. with that i end up throwing alot out because it would be too deep. this tek is really helpful though imo.
i plan on moving on soon from no pour agar, as the wedges are just so small and run out way to quick. but for the past few months i started on agar, they have served me well. id advise them for a noobie. im still a noobie but want to move up to larger shallower dishes, for both the ability to see from the top of a clear dish lid, and just bigger area to grow out on and hence, bigger wedges to inoculate jars with.
http://www.shroomery.org/forums/showflat.php/Number/16590981#16590981
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Big Gulps! Alright! Well, See ya later! And if i claim to be a wise man, well, it surely means that i dont know!
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Re: Does anybody else use small jars as petri dishes? [Re: DeadPhan]
#19242487 - 12/07/13 08:28 PM (10 years, 1 month ago) |
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I do no pour agar in a pp5 container all the time. Its easy and I can buy everything in town, no need to order shit off the net.
http://www.shroomery.org/forums/showflat.php/Number/19208976
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nn-IlliniSpiralDMT
Maniac


Registered: 07/19/12
Posts: 380
Last seen: 7 years, 3 months
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Re: Does anybody else use small jars as petri dishes? [Re: Psilosopherr]
#19242770 - 12/07/13 09:50 PM (10 years, 1 month ago) |
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Quote:
rbalzer said: wow, thanks for all the help guys! doing some reading now.
I can't find PP5 containers to buy by searching PP5 jars..
That's not the name for them it is just the plastic they are made out of... Search glad or zip lock brand screw-able plastic containers. You will find them and if not online they are sold virtually everywhere.
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Psilosopherr
A psilly goose



Registered: 02/15/12
Posts: 12,278
Last seen: 1 month, 10 days
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Re: Does anybody else use small jars as petri dishes? [Re: HypnotoadCroaked]
#19242814 - 12/07/13 10:05 PM (10 years, 1 month ago) |
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Quote:
Whippy said:
Quote:
rbalzer said:
I can't find PP5 containers to buy by searching PP5 jars..
Go to the grocery store, and look for name brand "glad" And "Ziplock"
(On the bottom of the jars it will have a #5 in a recycling triangle).

I prefer the 2 cup screw lids, and of the 2 brands I prefer the ziplock ONLY because the Glad have "deeper edges" than the center, while the ziplock brands have a flat bottom. This makes for slightly less agar per jar, AND if you are like me and grow cultures out to the edge (Im lazy), the edges wont be thick chunks. Does it make a difference? Probably not. ANYTHING with the pp5 logo will withstand the heat of the Pressure cooker. There is a world of various containers that are PP5. Find one you love, and own it!
ahh thank you! you've been quite helpful.
I didn't think common plastic containers would be made to withstand pressure cooking. What a convenient world (sometimes at least)
I'm gonna start searching for some to use for agar. Hoping I can find one with like 3 little trays (like the petri dishes divided into 3 sections) because that would make you guys jelly
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invitro


Registered: 05/03/13
Posts: 2,529
Last seen: 1 month, 20 days
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Re: Does anybody else use small jars as petri dishes? [Re: Psilosopherr]
#19242854 - 12/07/13 10:20 PM (10 years, 1 month ago) |
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I thought about getting some pp5 containers for agar work but one post said the extra air-space would hold too much oxygen and inhibit mycelial growth, as high co2 is the environment mycelium wants to grow in. I went to the store and only found 2 sizes, neither was flat like a petri dish, but they were as tall as they were wide.
Am I wrong about not wanting extra headspace in the pp5? Are there wide but short pp5 containers out there?
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HypnotoadCroaked
Retired, but will check MSGs

Registered: 01/05/13
Posts: 1,168
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Re: Does anybody else use small jars as petri dishes? [Re: invitro]
#19242899 - 12/07/13 10:36 PM (10 years, 1 month ago) |
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Quote:
invitro said:
Am I wrong about not wanting extra headspace in the pp5? Are there wide but short pp5 containers out there?
I don't know enough to tell you if you are wrong or not. I can tell you that I have had no issues at all with colonization speeds/times. I have tested this against unmodified lids and lids with a polyfill ge port.
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invitro


Registered: 05/03/13
Posts: 2,529
Last seen: 1 month, 20 days
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Re: Does anybody else use small jars as petri dishes? [Re: HypnotoadCroaked]
#19242921 - 12/07/13 10:42 PM (10 years, 1 month ago) |
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Interesting, can you see through the pp5 plastic wall well enough to be able to distinguish between mushroom and early signs of contam growth, which look similar.
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SuperFly
Still in the Space Race



Registered: 05/19/13
Posts: 1,032
Loc: Dark side off the moon
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Re: Does anybody else use small jars as petri dishes? [Re: invitro]
#19242964 - 12/07/13 10:51 PM (10 years, 1 month ago) |
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I use these for my no-pour agar 
They survive the PC and are only 1$ for 10
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HypnotoadCroaked
Retired, but will check MSGs

Registered: 01/05/13
Posts: 1,168
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Re: Does anybody else use small jars as petri dishes? [Re: SuperFly]
#19243207 - 12/08/13 12:12 AM (10 years, 1 month ago) |
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Quote:
invitro said: Interesting, can you see through the pp5 plastic wall well enough to be able to distinguish between mushroom and early signs of contam growth, which look similar.
I have inoculated with spores, LCs, and tissue, and any signs of contamination are visible. Granted I have never once used a real "petri dish". I have had no troubles whatsoever since I switched to this method.....More than likely I would do well with real dishes, but my reason for pp5 is 2 fold. I don't feel like ordering anything. Im cheap and they are available, re-useable, and can be done in a "No-pour-tek" fashion.
Quote:
TheMrMACdaddy said: I use these for my no-pour agar They survive the PC and are only 1$ for 10
Nice find there! Hell of a lot cheaper than the glad/ziplock things I have, but I do prefer the screw on lids versus the snap on. That is purely preference.
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Psilosopherr
A psilly goose



Registered: 02/15/12
Posts: 12,278
Last seen: 1 month, 10 days
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Re: Does anybody else use small jars as petri dishes? [Re: SuperFly]
#19243260 - 12/08/13 12:32 AM (10 years, 1 month ago) |
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Quote:
TheMrMACdaddy said: I use these for my no-pour agar 
They survive the PC and are only 1$ for 10
and it says "Be Fresh!"
Im sold
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bootster

Registered: 02/22/11
Posts: 1,531
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Re: Does anybody else use small jars as petri dishes? [Re: HypnotoadCroaked]
#19243270 - 12/08/13 12:40 AM (10 years, 1 month ago) |
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I tried to google them and it went nowhere.
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SuperFly
Still in the Space Race



Registered: 05/19/13
Posts: 1,032
Loc: Dark side off the moon
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Re: Does anybody else use small jars as petri dishes? [Re: Psilosopherr]
#19243290 - 12/08/13 12:53 AM (10 years, 1 month ago) |
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@ rbalzer If you can't find them and want the one I use me.
@Whippy I never used a real petri either. I've used the really small quarter pint (I think) mason jars, but after a couple clones or isolates I go through the jars fast. And on top of that Quote:
Whippy said:I don't feel like ordering anything. Im cheap and they are available, re-useable, and can be done in a "No-pour-tek" fashion.
couldn't have said it any better lol.
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