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InvisibleRaven44
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agar transfers clear growth after 24 hrs leading edge??
    #19241978 - 12/07/13 05:56 PM (10 years, 1 month ago)

I apologize if posting in the wrong section ahead of time. When making agar transfers, I noticed only a day after the transfer on some trays u can see very thin almost un noticeable new growth.but this growth has grown half a centimeter off the transferred agar piece. U have to tilt the tray and get it in the light right to be able to see it. Has anyone tried transferring's at this point rather than waiting until u see visible white growth? I'm curious before I go about doing this myself? is this simply referred as the leading edge of growth?? I'm curious weather anyone else has noticed this?? Its quite noticeable to myself. As I tried to explain before there is a nearly invisible 1/4 cm to full cm area of mycelium around the entire area of germination. U can see it from reflection of the light. I noticed this 24 he'd or less after making a transfer. There appeared to be no new growth, other than this growth in trying to explain. If I get no replies, I will go about this myself and post how it went... Also.. This new growth in trying to explain, has grown from transferred piece of agar, to new agar tray surface in 24 hrs or less. More like 12 hrs. So I'm asking if anyone has made transfers after only 12-24 hrs and how it went?


Edited by Raven44 (12/11/13 12:14 AM)


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Offlineleschampignons
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Re: agar transfers after 12-24 hrs? [Re: Raven44]
    #19244983 - 12/08/13 01:21 PM (10 years, 1 month ago)

Can we see a pic? This sorta sounds like it could possibly be bacteria. If you can see that it's definitely mycelium then transfer away. If its liquidy and/or you can't see any visible hyphae, you may just be transferring bacteria.


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OfflinePussyFart
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Re: agar transfers after 12-24 hrs? [Re: leschampignons]
    #19245174 - 12/08/13 02:00 PM (10 years, 1 month ago)

I know exactly what you are talking about, and I usually transfer that and actual white mycellium as well.

I have never tried to just transfer the absolute edge(the very thin almost clear shit)......I always grab actual white growth.....


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InvisibleRaven44
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Re: agar transfers after 12-24 hrs? [Re: PussyFart]
    #19245502 - 12/08/13 03:15 PM (10 years, 1 month ago)

Hmmm ok, sorry I won't be posting any pics anytime soon.. And prob couldn't capture what I'm talking about in a picture, not very easily at least I would guess.. I know its not bacteria IV had no contams its def mycelium on every tray.. I'm thinking u could transfer the thin almost clear ish and at least save some time and might be better practice I feel. I wonder if in doing so I'll have the same recovery time of 12-24 hrs now? And if not if its worth it in the long run... I will be trying this to see myself i think. Maybe on just a few trays next time i transfer


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InvisibleRaven44
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Re: agar transfers after 12-24 hrs? [Re: Raven44]
    #19245518 - 12/08/13 03:18 PM (10 years, 1 month ago)

Like I said this growth had made its way and could be ready yo transfer in 12-24 hrs. But if u wait for the noticeable white growth u end up waiting 2-4 days. Not that I'm in patient by any means just curious. I'm sure what I'm seeing is hyphae


Edited by Raven44 (12/08/13 03:48 PM)


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InvisibleRaven44
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Re: agar transfers after 12-24 hrs? [Re: Raven44]
    #19245546 - 12/08/13 03:24 PM (10 years, 1 month ago)

Also I'm doing multi spore syringe on fp agar in hopes of strain isolation in the future...


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Re: agar transfers after 12-24 hrs? [Re: Raven44]
    #19246925 - 12/08/13 07:59 PM (10 years, 1 month ago)

I think what you are seeing are metabolites released into the agar. Its a kinda clearish milky substance preceding the mycelium. I've seen this too and that's the conclusion I've come to


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OfflinePussyFart
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Re: agar transfers after 12-24 hrs? [Re: shopdropper]
    #19247039 - 12/08/13 08:26 PM (10 years, 1 month ago)

Quote:

shopdropper said:
I think what you are seeing are metabolites released into the agar. Its a kinda clearish milky substance preceding the mycelium. I've seen this too and that's the conclusion I've come to



No, it's got nothing to do with metabolites or contamination....it's just the absolute leading edge that is very very thin.


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InvisibleRaven44
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Re: agar transfers after 12-24 hrs? [Re: shopdropper]
    #19247052 - 12/08/13 08:29 PM (10 years, 1 month ago)

Yes its preceding the mycelium as u said, however I can observe growth individual lines or possibly hyhpae not sure the proper term.. They cross over each other and reach out. Look just like how mycelium grow, I think it is. I Will be transferring just a piece and agar and what I'm explaining soon and post how it goes. If I get any growth and what kind/ what it looks like blah blah


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InvisibleRaven44
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Re: agar transfers after 12-24 hrs? [Re: PussyFart]
    #19247066 - 12/08/13 08:31 PM (10 years, 1 month ago)

Also I will soon be making a transfer 12-24 hrs after transferring and let people know how that goes


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InvisibleRaven44
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Re: agar transfers after 12-24 hrs? [Re: Raven44]
    #19247084 - 12/08/13 08:34 PM (10 years, 1 month ago)

Roger rabbit says transfer the "very leading edge", stamets books say transfer asap pretty much, as soon as U can see growth or less than a dime in size.. It only makes sense, less time for contams to multiply, and it may be the optimum area of growth to select from


Edited by Raven44 (12/08/13 08:37 PM)


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OfflinePussyFart
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Re: agar transfers after 12-24 hrs? [Re: Raven44]
    #19247149 - 12/08/13 08:48 PM (10 years, 1 month ago)

Quote:

Raven44 said:
Roger rabbit says transfer the "very leading edge", stamets books say transfer asap pretty much, as soon as U can see growth or less than a dime in size.. It only makes sense, less time for contams to multiply, and it may be the optimum area of growth to select from



Yes, but if you watch RR's videos he transfers white growth, not the hard to see growth in front of the white growth.

And this is not a race, if there are contams on your dish you already lost.


Edited by PussyFart (12/08/13 08:48 PM)


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InvisibleRaven44
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Re: agar transfers after 12-24 hrs? [Re: PussyFart]
    #19247757 - 12/08/13 10:45 PM (10 years, 1 month ago)

Your thoughts considered. However I already stated in not impatient, quite patient in fact. My thoughts would be I don think anyone would try to make a video showing something u can't see and prob can't show in a bid and also masters may not give up every little secret of theirs. I'm working in front of a flow hood my friend not sure how ur thinking contam now I'm sure in nit talking about or dealing w any contamination.... I will post what happens after gaining first hand experience. Does anyone transfer what I'm speaking of and or do so after a period of 12- 24 hrs? I'm not too lazy to go about myself by any means. Curious and also making sure I'm not the only one who noticed this lol. Now I feel I won't be wasting anything, don like to waste. Resources or time


Edited by Raven44 (12/08/13 10:52 PM)


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Invisiblestonesun
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Re: agar transfers after 12-24 hrs? (moved) [Re: Raven44]
    #19247879 - 12/08/13 11:11 PM (10 years, 1 month ago)

This thread was moved from Advanced Mycology.

Reason:
Request


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InvisibleRaven44
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Re: agar transfers after 12-24 hrs? (moved) [Re: stonesun]
    #19258264 - 12/10/13 11:49 PM (10 years, 1 month ago)

For anyone who comes across this and cares to know, u can do as I thought in this post. However I believe the recovery time if 12 hrs was partially due to the excessive amount of mycelium I had transferred. Also there was an area on one tray of mine that had this clear growth I described, and it extended 3/4" out. I noticed some trays have thus clear growth that extends out in spots an extra ordinary amount. I would select from this and would think it would be the "leading edge". I transferred from the tray w clear growth 3/4" from any visibly white growth. Those trays are recovering slower but I feel won't be too far behind trays done at the same time w a very small piece of actual visible white growth.. The parent was two or three days old.. So pretty much as soon as u can see growth (doesn't have to b white..) u can transfer. May b better to wait until u can see more of the characteristics of the growth tho... I dunno??


Edited by Raven44 (12/11/13 12:18 AM)


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InvisibleRaven44
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Re: agar transfers after 12-24 hrs? (moved) [Re: Raven44]
    #20047397 - 05/27/14 03:15 PM (9 years, 8 months ago)

So here we go here is the great discovery lol, i have much to learn. Do not transfer the hyphae, i believe that is what the clearish growth is called i was referring to. Someone correct me if I'm wrong. You want to transfer white rhizomorphic growth as so many have prob already stated.


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