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InvisiblecArcace-x
YeAh!!
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Registered: 10/17/13
Posts: 2,412
Loc: The Simulator
Re: lab sterilization [Re: Ultron]
    #19241599 - 12/07/13 03:58 PM (10 years, 2 months ago)

a lighter torch can be usefull, yes it's a flame but not always lit, and heat your tool fast


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OfflineKizzle
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Registered: 08/30/11
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Re: lab sterilization [Re: cArcace-x]
    #19241701 - 12/07/13 04:31 PM (10 years, 2 months ago)

There are electric devices for sterilizing needles and inoculation loops. I'm not sure if they would work on scalpels.


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OfflineOICU812
NC Tree Farm owner
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Registered: 11/06/11
Posts: 1,064
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Re: lab sterilization [Re: Ultron]
    #19241738 - 12/07/13 04:42 PM (10 years, 2 months ago)

Quote:

jonathan9301 said:
i wonder if there is a flame less solution for heating tools to red hot




  • Drill a 1" hole in the side of the box about 1" above your lamp wick height. 
  • Place the flame close to the box at the hole. 
  • Stick the scalpel through the hole into the flame. 
  • Heat the blade to a orange/red glow. 
  • Withdraw the scalpel back into the box. 
  • Cool the scalpel blade in the new agar.
  • Proceed with the transfer.
  • Repeat for each transfer.


The blade should remain hot enough to kill any contamination falling on it between the flame and the inside of the SAB.

A lazy (smart) person can even fabricate a tool rest to hold the scalpel while the blade is heating in the flame.


Edited by OICU812 (12/07/13 04:44 PM)


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Invisible36fuckin5
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Registered: 08/11/03
Posts: 12,081
Loc: Diving into Mystical Territori...
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Re: lab sterilization [Re: OICU812]
    #19241892 - 12/07/13 05:31 PM (10 years, 2 months ago)

While cutting an extra hole in your SAB would probably work, it's just extra work that's not needed. You can just take your hand out of the box to flame. Making a stand, while small, takes up the precious little space in your SAB.


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Redd Foxx said:
If you're offended I don't give a shit and don't come see me no more.

Pat The Bunny said:
A punk rock song won't ever change the world, but I can tell you about a couple that changed me.

bodhisatta said:
i recommend common sense and figuring it out.

These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.


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OfflineUltron
alchemist programmer
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Re: lab sterilization [Re: 36fuckin5]
    #19241907 - 12/07/13 05:35 PM (10 years, 2 months ago)

i wouldn't make a stand but the hole to flame sterilize without removing hands is a good idea


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Invisible36fuckin5
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Registered: 08/11/03
Posts: 12,081
Loc: Diving into Mystical Territori...
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Re: lab sterilization [Re: Ultron]
    #19241967 - 12/07/13 05:54 PM (10 years, 2 months ago)

The hole would work, but I don't see the point in an extra hole in your clean box when just pulling your arm out works just fine. You would also have to keep the area with the hole constantly clear, which would limit some space and make shit awkward.


--------------------
Redd Foxx said:
If you're offended I don't give a shit and don't come see me no more.

Pat The Bunny said:
A punk rock song won't ever change the world, but I can tell you about a couple that changed me.

bodhisatta said:
i recommend common sense and figuring it out.

These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.


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OfflineOICU812
NC Tree Farm owner
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Registered: 11/06/11
Posts: 1,064
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Re: lab sterilization [Re: 36fuckin5]
    #19242055 - 12/07/13 06:17 PM (10 years, 2 months ago)

Taking hands in and out of the SAB either requires re-sanitation or increases the risk of introducing contaminants into the process.








Ron White was right!


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"Democracy is two wolves and a lamb voting on what to have for lunch. Liberty is a well-armed lamb contesting the vote!" --Benjamin Franklin

"Those who give up liberty for security won't have, or deserve, either.". . . Benjamin Franklin
----> Read: The Fight of our Lives - Defeating the Ideological War Against the West - by Victor Davis Hanson


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OfflinePussyFart
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Re: lab sterilization [Re: OICU812]
    #19242101 - 12/07/13 06:31 PM (10 years, 2 months ago)

Quote:

OICU812 said:
Taking hands in and out of the SAB either requires re-sanitation



No it does not...

Quote:

OICU812 said:
or increases the risk of introducing contaminants into the process.



Not this either.

We allow the air to settle before going back to work.

Any contams that made their way in fall to the floor of the SAB....and I like to have a wet towel in the bottom....so nothing can bounce back up.


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InvisibleJ. Jack Flash
stranger than ever.


Registered: 11/20/13
Posts: 1,500
Re: lab sterilization [Re: PussyFart]
    #19253104 - 12/09/13 10:50 PM (10 years, 2 months ago)

I just finished my SAB and i noticed something interesting.  I used a couple 4" PVC fittings with the threaded plugs, right?  Silicone stuck them in place.  Anyway, i noticed when i shoved my arms through them, just to try it on so to speak, the hair on my arms stood up like i was about to get struck by lightning.  The PVC fittings, heavy heavy plastic such as they are, built up a great deal of static.  Seems like that would act on potential contaminants being brought in from outside as well as floating around inside, by attracting them to and trapping them on the plastic fittings.  Seem reasonable?  Sure makes me feel better about the effort to build the damn thing.
:cool:


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the j stands for jesus.
2020 new years grow along


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OfflineAmanita virosa
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Re: lab sterilization [Re: J. Jack Flash]
    #19254123 - 12/10/13 06:44 AM (10 years, 2 months ago)

Except the amount of static will be directly related to relative humidity.  You won't always have it. Don't electrocute urself!  :cool:


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OfflineTerry M
Stranger in a Strange Land
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Registered: 06/18/10
Posts: 1,502
Loc: Rhode Island
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Re: lab sterilization [Re: J. Jack Flash]
    #19254953 - 12/10/13 11:37 AM (10 years, 2 months ago)

Quote:

J. Jack Flash said:
I just finished my SAB and i noticed something interesting.  I used a couple 4" PVC fittings with the threaded plugs, right?  Silicone stuck them in place.  Anyway, i noticed when i shoved my arms through them, just to try it on so to speak, the hair on my arms stood up like i was about to get struck by lightning.  The PVC fittings, heavy heavy plastic such as they are, built up a great deal of static.  Seems like that would act on potential contaminants being brought in from outside as well as floating around inside, by attracting them to and trapping them on the plastic fittings.  Seem reasonable?  Sure makes me feel better about the effort to build the damn thing.
:cool:




You should really use Tyvek sleeves right up to your gloves for sterility. The sleeves are reusable almost indefinitely, until the elastic at each end completely gives out.


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InvisibleJ. Jack Flash
stranger than ever.


Registered: 11/20/13
Posts: 1,500
Re: lab sterilization [Re: Amanita virosa]
    #19257031 - 12/10/13 06:59 PM (10 years, 2 months ago)

Yep, tyvek up to the gloves.  I tried making some sleeves from shipping envelopes, failed miserably, then just bought some.  Pretty cool stuff.  got the surgical masks, too.

Meanwhile, i've been reading about this static and airborne contaminants thing, and yeah, the humidity issue is interesting. 
http://www.trifield.com/content/tribo-electric-series/
distilled, it says which things rubbed together make what relative amount of static.  PVC is super negative, while PU is super postitive.  so maybe rubbing a polyurethane bushing against the PVC and Tyvek (which i think is PE) and it generates large charge, like balloon against head.  if the box is swimming in alcohol vapor, will that affect static same as water vapor, i wonder? 
regardless of practicality, it's fun to think about.

here's a pilot study whose results suggest static formed on plastic hospital aprons is contributing to high infection rates in immune compromised patients.  the static on nurses' aprons attracts bacteria, which are then transmitted to patients as care is given.

and this makes the claim that bacteria are generally on the negative end.  suggests to me that PU would be better to have for the arm collars(?), as they charged positive so they'd attract the negative charged bacteria from the air

anyway, just geeking out a lil.  :rolleyes:


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the j stands for jesus.
2020 new years grow along


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