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UnknownPerson
Stranger
Registered: 10/19/13
Posts: 50
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Multispore vs. Lc cloning *DELETED*
#19241706 - 12/07/13 04:32 PM (10 years, 2 months ago) |
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Post deleted by UnknownPersonReason for deletion: OLD
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PussyFart
Retired Cultivation Extrodinaire



Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
Last seen: 1 month, 11 days
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Quote:
UnknownPerson said: I was wondering if it would be more beneficial to inoculate more grain jars with the LC from the multi spore syringe, or to make a new LC using this TEK: http://www.shroomery.org/forums/showflat.php?Cat=0&Number=5874305&page=0&vc=1
Taking a biopsy of the biggest and healthiest looking mushroom? Thoughts?
Inoculating the jars with the spores and skipping the useless/risky step of making an LC would be the best option honestly.
If you knock up 10 grain jars now and G2G them out, AND make an LC, you would have 100 grain jars fully colonized and ready to go by the time the LC is ready and tested, and then you would still have to wait for the grains to colonize, granted the LC was clean.
LC is never the best option for cubes, especially when talking about making an LC from spores...no spore syringe is ever 100% clean, and LC is the perfect breeding ground for everything not mycellium....hence why it is so sketchy.
The only LC I would trust would be an LC made from a clean agar wedge.....
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THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT! A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD, SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List, EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE *** *** AFGHAN KUSH GROW LOG *** ***
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UnknownPerson
Stranger
Registered: 10/19/13
Posts: 50
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Thanks for the input. I will do some searching, but do you have a TEK in mind ?
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PussyFart
Retired Cultivation Extrodinaire



Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
Last seen: 1 month, 11 days
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--------------------
THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT! A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD, SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List, EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE *** *** AFGHAN KUSH GROW LOG *** ***
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bodhisatta 
Smurf real estate agent



Registered: 04/30/13
Posts: 61,890
Loc: Milky way
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Re: Multispore vs. Lc cloning [Re: PussyFart]
#19242120 - 12/07/13 06:36 PM (10 years, 2 months ago) |
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Here is some reading material for the time beeing. you should make spore syringes from prints to keep your cultivation rolling around until you feel like getting into agar.
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UnknownPerson
Stranger
Registered: 10/19/13
Posts: 50
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Yeah I am not ready to get into agar with it being my first grow. I have actually done G2G once in my SAB and been successful. So I guess my question now would be does anyone have any tek suggestions for inoculating a jar using spores from a fresh mushroom. I've seen ones on how to make a spore syringe from a spore print . Is the first step to make a spore print?
EDIT: Also using spores from what I have growing right now would be more beneficial then using a multi spore syringe I got online and/or a LC made from a multi spore syringe?
Edited by UnknownPerson (12/07/13 08:40 PM)
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bodhisatta 
Smurf real estate agent



Registered: 04/30/13
Posts: 61,890
Loc: Milky way
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Quote:
UnknownPerson said: Yeah I am not ready to get into agar with it being my first grow. I have actually done G2G once in my SAB and been successful. So I guess my question now would be does anyone have any tek suggestions for inoculating a jar using spores from a fresh mushroom. I've seen ones on how to make a spore syringe from a spore print . Is the first step to make a spore print?
EDIT: Also using spores from what I have growing right now would be more beneficial then using a multi spore syringe I got online and/or a LC made from a multi spore syringe?
agar is easy and really helps you get better at sterile tek
spore syringes come from a spore print you'll have to print a cap to get the spores. RR has a good video in the let's grow mushroom series.
spores that you print from mushrooms you grow are the exact same as ones you would get from a vendor you just have to be pretty clean about it but trust me it's not that hard if you follow the directions.
I would advice to skip a LC all together Click that and read through there.
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chucklehead
GeneticThrowback toNeanderthal



Registered: 02/17/07
Posts: 1,338
Loc: Chucky Cheese
Last seen: 4 years, 8 months
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Re: Multispore vs. Lc cloning [Re: bodhisatta]
#19243742 - 12/08/13 05:40 AM (10 years, 2 months ago) |
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Look up grocery store agar tek. I have used the LC cloning tek you are talking about with some success. I am not going to poo poo LC. They had their uses for me but they become obsolete and undesirable after you learn agar. The number one reason for my preference is relative certainty of having a clean culture versus crap shoot in a bottle. Second reason is I hate making syringes. It is a pain in The ass. FYI spores to agar and spores to LC are equally capable of contaminating. The differences are these: on agar you can transfer away form the problem, LC you can't do that so easily. I know you are new but go from first grow to playing with the big boys. So clone your fruits. In a week you potentially could inoculate grain with your clones. Though I suggest three transfer away from the original.
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