Home | Community | Message Board

MagicBag Grow Bags
This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: North Spore Bulk Substrate   Myyco.com Isolated Cubensis Liquid Culture For Sale   Mushroom-Hut Liquid Cultures   Left Coast Kratom Buy Kratom Extract

Jump to first unread post Pages: < Back | 1 | 2  [ show all ]
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
Offlinemyo6
Stranger

Registered: 06/06/13
Posts: 21
Last seen: 8 years, 9 months
Re: Spores germinate on agar but they didn't on grain [Re: 36fuckin5]
    #19237263 - 12/06/13 04:17 PM (10 years, 2 months ago)

Wow,thanks for all this replies!

I have some facts to add:
-You can buy some
-I previously used this grain tek,with great success,for inoculation with agar wedges,or G2G;I know it can't be my grain tek.
-I shaked the jars immediatly after inoculation;
-I'm using a glovebox and a sterile scalpel,I DIDN'T FLAME MY SCALPEL: it's not a burnt spores problem.
-Also,I don't think my tap water is the problem,I dunk all my substrate/make LC's/use it to soak my grain/to moisten casing substrate/ with no problems....


Edited by myo6 (05/09/15 04:45 AM)


Extras: Filter Print Post Top
OfflinecronicrFacebook
 User Gallery


Registered: 08/07/11
Posts: 61,436
Loc: Van Isle Flag
Last seen: 2 years, 1 month
Trusted Cultivator
Re: Spores germinate on agar but they didn't on grain [Re: myo6]
    #19237287 - 12/06/13 04:21 PM (10 years, 2 months ago)

shouldn't shake after innoculating your jars especialy with only 1 cc
the things you listed above are all fine with tap water but spores are sometimes the exception, distilled is what you want


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


Extras: Filter Print Post Top
OfflineTheMustardTiger
Male

Registered: 08/19/13
Posts: 4,700
Loc: Sunnyvale Trailer Park
Last seen: 6 years, 6 months
Re: Spores germinate on agar but they didn't on grain [Re: cronicr]
    #19237315 - 12/06/13 04:28 PM (10 years, 2 months ago)

Quote:

cronicr said:
shouldn't shake after innoculating your jars especialy with only 1 cc
the things you listed above are all fine with tap water but spores are sometimes the exception, distilled is what you want




:thumbup:


--------------------


Extras: Filter Print Post Top
Invisibleanne halonium
jaguarette
Female

Registered: 05/07/13
Posts: 1,908
Re: Spores germinate on agar but they didn't on grain [Re: TheMustardTiger]
    #19237325 - 12/06/13 04:30 PM (10 years, 2 months ago)

ya , im shaky on the shaking thing also.........
second i saw that, im like.....now thats shaky.

bacteria in jars.

:cookiemonster:


--------------------
:aliendance:


Edited by anne halonium (12/06/13 04:33 PM)


Extras: Filter Print Post Top
InvisiblePastywhyteMDiscord
Say hello to my little friend
Male User Gallery


Registered: 09/15/12
Posts: 37,811
Loc: Canada Flag
Trusted Cultivator
Re: Spores germinate on agar but they didn't on grain [Re: SpitballJedi]
    #19237413 - 12/06/13 04:47 PM (10 years, 2 months ago)

Quote:

SpitballJedi said:
He went from print to agar with success.

He went from print to syringe to agar and grain without success.

He only mentioned the scalpel for making the syringe.

I would like to hear more about the steps when using the scalpel.




Extras: Filter Print Post Top
Offlinemyo6
Stranger

Registered: 06/06/13
Posts: 21
Last seen: 8 years, 9 months
Re: Spores germinate on agar but they didn't on grain [Re: Pastywhyte]
    #19239089 - 12/06/13 11:49 PM (10 years, 2 months ago)

Quote:

Pastywhyte said:
Quote:

SpitballJedi said:
He went from print to agar with success.

He went from print to syringe to agar and grain without success.

He only mentioned the scalpel for making the syringe.

I would like to hear more about the steps when using the scalpel.






I put all the supplies needed in my glovebox,and I used alcohol to mist the inside of the GB before close it and let it stand 10 minutes;
I openened my aluminium print foil;
I opened my sterile scalpel blister;
I carefully scraped some spores with it;
I let it fall on the sterilized erlenmeyer water/I touched the center of the agar plate with it.

That's what Im doing when using a print!


Extras: Filter Print Post Top
Offlinemyo6
Stranger

Registered: 06/06/13
Posts: 21
Last seen: 8 years, 9 months
Re: Spores germinate on agar but they didn't on grain [Re: myo6]
    #19243521 - 12/08/13 03:06 AM (10 years, 2 months ago)

bump


Extras: Filter Print Post Top
InvisiblePastywhyteMDiscord
Say hello to my little friend
Male User Gallery


Registered: 09/15/12
Posts: 37,811
Loc: Canada Flag
Trusted Cultivator
Re: Spores germinate on agar but they didn't on grain [Re: myo6]
    #19244366 - 12/08/13 10:38 AM (10 years, 2 months ago)

Quote:

myo6 said:
I put all the supplies needed in my glovebox,and I used alcohol to mist the inside of the GB before close it and let it stand 10 minutes;
I openened my aluminium print foil;
I opened my sterile scalpel blister;
I carefully scraped some spores with it;
I let it fall on the sterilized erlenmeyer water/I touched the center of the agar plate with it.

That's what Im doing when using a print!




Quote:

Pastywhyte said:
I have many times seen germination on agar outpace germination on grains or brf. Agar is the ideal media for germination of pretty much anything, brf and grains not so much. I have had have some jars inoculated with MS take over two weeks to show growth. Meanwhile the plates I had inoculated at the same time had already been transferred once and master knocked up with wedges. Many times those masters will finish well before the one inoculated with ms solution.

This is all just my experience but I advise a little patience. No matter what, I would wager your plates will provide the better culture in the long run.




Extras: Filter Print Post Top
Jump to top Pages: < Back | 1 | 2  [ show all ]

Shop: North Spore Bulk Substrate   Myyco.com Isolated Cubensis Liquid Culture For Sale   Mushroom-Hut Liquid Cultures   Left Coast Kratom Buy Kratom Extract


Similar ThreadsPosterViewsRepliesLast post
* spores germination on cardboard (pics) comario2 7,971 3 10/12/17 04:29 PM
by AK49
* Spores on agar question .More info plz tripndicular 4,373 5 09/13/02 05:27 PM
by tripndicular
* Will my year old spores germinate? case 2,643 2 08/16/01 02:49 PM
by shellacct
* spore germination Anonymous 1,768 1 10/08/02 02:04 PM
by Skikid16
* can spores germinate in water? XOCHI 2,328 2 06/26/02 05:53 AM
by Mycelium
* Re: How do I use a sporeprint versus the normal spore syringe? Anonymous 2,320 11 07/26/00 09:54 AM
by psilocyber
* Benefits of Agar? celsius 9,418 10 12/01/19 12:36 AM
by wildernessjunkie
* agar meatman 1,761 9 09/14/02 05:32 AM
by meatman

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
1,745 topic views. 22 members, 211 guests and 29 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.027 seconds spending 0.007 seconds on 13 queries.