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OfflineCAP_TURTLE
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Slower than usual agar plates
    #19237442 - 12/06/13 04:56 PM (10 years, 1 month ago)

I recently started using petri dishes instead of no pour plates and with my first attempt everything has been super slow to grow.  I am doing a wide variety of species from H. corraloides to P. eryngii.  The only ones that went super fast were the King oysters and the Turkey tail.  I have an agressive P. columbinus that is slow to jump to the agar and growing very slow.  Taking weeks.  No other contams appear to be present but maybe I am wrong.  I did agar to grains a coupld days ago so that may be a tell.  But I need to make some more plates in the mean time.  Am I right to parafilm right after inoculating plates?  I use 500ml water 10g agar 10g lme.  Same as i've always used except I did breather holes in the no pour plates I used to do.  I used an old alcohol bottle but I rinsed it well to cook the agar.  Another thing I did was cook the agar for 45 mins after reading others doing it this long.  With the no pour plates I used to go 20 mins but the layers were much thinner than a whole bottle.  Also the agar was poured a tad bit thicker in the plates than the no pours i used to do.  Maybe this slows it down greater? Maybe the sugars are old? I've had the same batch for 2 years.  Temps are as usual.  Not sure what else could be it but Any ideas are appreciated.


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Invisibleliamtheloser
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Re: Slower than usual agar plates [Re: CAP_TURTLE]
    #19237602 - 12/06/13 05:31 PM (10 years, 1 month ago)

Maybe try using a different recipe.  Like a yeast based recipe or dextrose one.. Try potato flake or nutrient yeast or something handy, see if they take off on those... I have some reishi that got bored of my usual agar recipe so I had to change it up a little.


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OfflineOICU812
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Re: Slower than usual agar plates [Re: liamtheloser]
    #19237782 - 12/06/13 06:08 PM (10 years, 1 month ago)

Be aware that low temps affect rate of growth.  Maintaining about 72°F. might help.  Nothing unusual about your recipe.  You might want to bookmark Fast Fred's Media cookbook.  It's a classic and has some good recipes in it.

I generally use 20mg. agar and 20mg LME to 1/2 L. of water, but I like my agar plates firm (like my women). 

You are doing OK, just keep pourin'.


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"Democracy is two wolves and a lamb voting on what to have for lunch. Liberty is a well-armed lamb contesting the vote!" --Benjamin Franklin

"Those who give up liberty for security won't have, or deserve, either.". . . Benjamin Franklin
----> Read: The Fight of our Lives - Defeating the Ideological War Against the West - by Victor Davis Hanson


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OfflineJeff
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Re: Slower than usual agar plates [Re: OICU812]
    #19237808 - 12/06/13 06:14 PM (10 years, 1 month ago)

That is definitely a rich recipe.  Firm....I do believe that.  :smile:


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OfflineCAP_TURTLE
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Re: Slower than usual agar plates [Re: Jeff]
    #19237894 - 12/06/13 06:33 PM (10 years, 1 month ago)

PDA was an alternate I was considering.  It was just oddly slow almost insanely slow but it all colonized.  Only difference was plates over no pours.  Thanks for the input and encouragement.  Plates are fun.  Seem like more work but its nice to have a fully visible plate over looking through the sides of a no pour or making a clear lid.  I will keep on trucking!


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