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OfflineLittleDaddy
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Beginner PF Tek, Need Reassurance
    #19236663 - 12/06/13 01:45 PM (10 years, 1 month ago)

So I accidentally shipped the spores to my mom (confused billing and shipping address). lol Got quite the funny text asking why I ordered a syringe of Anti-Hangover drugs hahaha. Anyways, told her I was working on a sporeprint project for bio and got them expedited to me.

That aside, I finished the substrate. I used half pint Kerr jars, flipped the inner lid upside down, cut 4 small holes with a knife, duct taped them closed, and poured the nutrients in. I wasn't being meticulous and I only wiped the top of one jar before inserting the dry vermiculite. So I foiled up the jars and elevated them in the pot and steamed them for an hour. Now, they are waiting until I receive the spore syringe either today (unlikely because of snow) or Monday (maybe unlikely then, too).

So I steamed them and I can't inoculate them for at least 3 days and I don't know if not wiping the top of the jar before putting the dry vermiculite will really cause contamination (I didn't get the brown rice vermiculite mixture on the sides when pouring it in). I can restart if needbe, but I'd prefer not.

Opinions from credible PF tekers?


--------------------
The hotter the battle, the sweeter Jah victory.
Put the heathen's back upon the wall.


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OfflinecronicrFacebook
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Re: Beginner PF Tek, Need Reassurance [Re: LittleDaddy]
    #19236668 - 12/06/13 01:47 PM (10 years, 1 month ago)

you shouldn't have taped your holes with duct tape


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It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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OfflineLittleDaddy
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Re: Beginner PF Tek, Need Reassurance [Re: cronicr]
    #19236702 - 12/06/13 01:52 PM (10 years, 1 month ago)

How will it affect it?


--------------------
The hotter the battle, the sweeter Jah victory.
Put the heathen's back upon the wall.


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OfflinecronicrFacebook
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Re: Beginner PF Tek, Need Reassurance [Re: LittleDaddy]
    #19236710 - 12/06/13 01:55 PM (10 years, 1 month ago)

well you essentially plugged it for the sterilizing process, the only thing that may have saved you was the upside down lid, i also steam mine for 90 minutes and wipe before adding the dry verm


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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OfflineLittleDaddy
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Re: Beginner PF Tek, Need Reassurance [Re: cronicr]
    #19236723 - 12/06/13 01:57 PM (10 years, 1 month ago)

Quote:

cronicr said:
well you essentially plugged it for the sterilizing process, the only thing that may have saved you was the upside down lid, i also steam mine for 90 minutes and wipe before adding the dry verm




So there shouldn't be ANY tape covering during the sterilization process? Should I restart?

And yeah, I sterilized them for about 70-90


--------------------
The hotter the battle, the sweeter Jah victory.
Put the heathen's back upon the wall.


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OfflineOgreLokon
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Re: Beginner PF Tek, Need Reassurance [Re: cronicr]
    #19236737 - 12/06/13 02:00 PM (10 years, 1 month ago)

I forgot to wipe down my last batch of jars' rims before steaming, and only one contamed. NOT the best practice, but also not the end of the world.

:2cents:


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OfflinecronicrFacebook
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Re: Beginner PF Tek, Need Reassurance [Re: LittleDaddy]
    #19236742 - 12/06/13 02:01 PM (10 years, 1 month ago)

there probly fine as long as they were not air tight enough to create a vaccum that will suck in air when you remove the tape


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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OfflineLittleDaddy
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Re: Beginner PF Tek, Need Reassurance [Re: cronicr]
    #19236771 - 12/06/13 02:09 PM (10 years, 1 month ago)

Thank you, fellas.

Will it be find to hold over a few days, or will I have to sterilize them again?

Also, what should I expect from the water? Vapor on the sides or no activity until it's inoculated?


--------------------
The hotter the battle, the sweeter Jah victory.
Put the heathen's back upon the wall.


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OfflinecronicrFacebook
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Re: Beginner PF Tek, Need Reassurance [Re: LittleDaddy]
    #19236787 - 12/06/13 02:12 PM (10 years, 1 month ago)

they will be fine for a few days, got no idea about the vapour q though? what are you talking about


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It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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OfflineLittleDaddy
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Re: Beginner PF Tek, Need Reassurance [Re: cronicr]
    #19236798 - 12/06/13 02:14 PM (10 years, 1 month ago)

Like should I expect to see vapor collect on the side of the jar (like fog) and if so, how much?


--------------------
The hotter the battle, the sweeter Jah victory.
Put the heathen's back upon the wall.


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Re: Beginner PF Tek, Need Reassurance [Re: LittleDaddy]
    #19236803 - 12/06/13 02:15 PM (10 years, 1 month ago)

you may see some condensation build up as it colonizes but thats fine


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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OfflineLittleDaddy
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Re: Beginner PF Tek, Need Reassurance [Re: cronicr]
    #19236810 - 12/06/13 02:17 PM (10 years, 1 month ago)

Okay, thank you very much. I appreciate all your help.


--------------------
The hotter the battle, the sweeter Jah victory.
Put the heathen's back upon the wall.


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OfflinecronicrFacebook
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Re: Beginner PF Tek, Need Reassurance [Re: LittleDaddy]
    #19236817 - 12/06/13 02:19 PM (10 years, 1 month ago)

for future refrence most use micropore tape for there holes but it's optional, you can leave the holes open and cover with foil to stop water getting in


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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OfflineLittleDaddy
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Re: Beginner PF Tek, Need Reassurance [Re: cronicr]
    #19236825 - 12/06/13 02:21 PM (10 years, 1 month ago)

Hmm, I was wondering why I covered it with foil. That makes sense. I may resterilize.


--------------------
The hotter the battle, the sweeter Jah victory.
Put the heathen's back upon the wall.


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Offline1down5up
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Re: Beginner PF Tek, Need Reassurance [Re: LittleDaddy]
    #19236934 - 12/06/13 02:51 PM (10 years, 1 month ago)

If you use the dry verm layer on top, you shouldn't have to worry too much about the tape.  The dry verm layer is there to catch contams.  It frees up the holes to allow for more GE too.


--------------------
~~Everything is relative~~


A Simplified Overview of Mushroom Cultivation Strategies  -  RR says  -  EvilMushroom666's Take on BRF Cakes  -  Frank's list of goodies  -  Cronicr's Goodies


No one is placed higher than another no matter race or creed or gender, we must teach forgiveness and compassion for all life.  J.L.


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Invisibleanne halonium
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Re: Beginner PF Tek, Need Reassurance [Re: 1down5up]
    #19236992 - 12/06/13 03:00 PM (10 years, 1 month ago)

i can " assure " OP,
youll do it all different next time.

you can be "reassured", if ya get a Pc, youll thank me.


--------------------
:aliendance:


Edited by anne halonium (12/06/13 03:02 PM)


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OfflineLittleDaddy
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Re: Beginner PF Tek, Need Reassurance [Re: anne halonium]
    #19236998 - 12/06/13 03:02 PM (10 years, 1 month ago)

Not sure I understand what you said. And I don't know what a Pc is. Would you mind rephrasing?


--------------------
The hotter the battle, the sweeter Jah victory.
Put the heathen's back upon the wall.


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Invisibleanne halonium
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Re: Beginner PF Tek, Need Reassurance [Re: LittleDaddy]
    #19237003 - 12/06/13 03:03 PM (10 years, 1 month ago)

get a pressure cooker, and consider other teks.
also, dont mail stuff to moms.....


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:aliendance:


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OfflineLittleDaddy
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Re: Beginner PF Tek, Need Reassurance [Re: anne halonium]
    #19237025 - 12/06/13 03:10 PM (10 years, 1 month ago)

yeah, pressure cookers definitely seem like the better option.

lol, that was quite the mistake. oh, well. got out of it.


--------------------
The hotter the battle, the sweeter Jah victory.
Put the heathen's back upon the wall.


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OfflineLittleDaddy
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Re: Beginner PF Tek, Need Reassurance [Re: LittleDaddy]
    #19237041 - 12/06/13 03:16 PM (10 years, 1 month ago)

I also realized that I poured water after I had mixed the vermiculite and brown rice. Will this affect anything?


--------------------
The hotter the battle, the sweeter Jah victory.
Put the heathen's back upon the wall.


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Re: Beginner PF Tek, Need Reassurance [Re: LittleDaddy]
    #19237046 - 12/06/13 03:16 PM (10 years, 1 month ago)

nope thats fine


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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OfflineLittleDaddy
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Re: Beginner PF Tek, Need Reassurance [Re: cronicr]
    #19237104 - 12/06/13 03:30 PM (10 years, 1 month ago)

I'm just gonna restart. Too many variables. Good learning experience, regardless.


--------------------
The hotter the battle, the sweeter Jah victory.
Put the heathen's back upon the wall.


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Invisibleanne halonium
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Re: Beginner PF Tek, Need Reassurance [Re: LittleDaddy]
    #19237141 - 12/06/13 03:40 PM (10 years, 1 month ago)

well, hold out til ya see contams at least.

theres proof  a plenty,
that even the worst grows, can amaze ya sometimes.


--------------------
:aliendance:


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OfflineLittleDaddy
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Re: Beginner PF Tek, Need Reassurance [Re: anne halonium]
    #19237177 - 12/06/13 03:50 PM (10 years, 1 month ago)

Quote:

anne halonium said:
well, hold out til ya see contams at least.

theres proof  a plenty,
that even the worst grows, can amaze ya sometimes.




will do, man.


--------------------
The hotter the battle, the sweeter Jah victory.
Put the heathen's back upon the wall.


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Re: Beginner PF Tek, Need Reassurance [Re: LittleDaddy]
    #19237227 - 12/06/13 04:08 PM (10 years, 1 month ago)

yeah bro just ride it out and get some more jars started in the mean time, keeping busy helps the patients party of it all, and since annies been so nice lately i'll even suggest you get some pp5's instead of jars they work great for cakes


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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Offline2bittoker
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Re: Beginner PF Tek, Need Reassurance [Re: cronicr]
    #19237451 - 12/06/13 04:58 PM (10 years, 1 month ago)

Quote:


That aside, I finished the substrate. I used half pint Kerr jars, flipped the inner lid upside down, cut 4 small holes with a knife,



A drill works far better, and will be needed for making sure you're making the right sized holes in your SGFC.  At 12V black and decker and a drill bit set can be got at walmart for $25.
Quote:


duct taped them closed,



Micropore tape man, get it on a tape dispenser at CVS for $5.  Get 3M micropore tape, also called Nexcare Gentle Paper Tape.
Quote:

and poured the nutrients in. I wasn't being meticulous and I only wiped the top of one jar before inserting the dry vermiculite.




Wipe after doing dry verm barrier.  The point of wiping is to make sure no particles prevent the lid from creating a seal, opening a vector for contams.

Quote:


So I foiled up the jars and elevated them in the pot and steamed them for an hour. Now, they are waiting until I receive the spore syringe either today (unlikely because of snow) or Monday (maybe unlikely then, too).


If you are doing the steam bath method, do not make and sanitize the jar until you are ready to inoculate. Steam bathing is different than pressure cooking in that the temperatures needed for autoclaving something cannot be reached in a boiling pot due to thermal energy release in steam. Steam sanitation only zaps most of the microbials and stuns the rest, long enough for the mushroom mycelium to overtake the jar.

Autoclaving with a pressure cooker at 15PSI is the only way to achieve sufficient enough sterility for short term storage of BRF cakes.   
Quote:


So I steamed them and I can't inoculate them for at least 3 days and I don't know if not wiping the top of the jar before putting the dry vermiculite will really cause contamination (I didn't get the brown rice vermiculite mixture on the sides when pouring it in). I can restart if needbe, but I'd prefer not.

Opinions from credible PF tekers?


I would never trust a steam sanitized jar that wasnt knocked up immediately.  You've shortened the window that the mycelium has to colonize before the contams that werent killed by the steam recover and begin colonizing.

Good luck bro.  Hope it works out for ya. :mushroom2:


--------------------
“I slept and dreamt that life was joy. I awoke and saw that life was service. I acted and behold, service was joy.”
"Love does not claim possession, but gives freedom"
― Rabindranath Tagore

Stuff for New Growers
Where new growers should start: RogerRabbit's PF Tek video    How it Should and Shouldn't Look 
My Simplified Bulk Growing  My OJ Shroom Tek


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OfflineLittleDaddy
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Re: Beginner PF Tek, Need Reassurance [Re: 2bittoker]
    #19237483 - 12/06/13 05:06 PM (10 years, 1 month ago)

Very informative. Thank you. Couple questions.

What is SFGC? I'm new to all these acronyms lol
Next, do I have to restart the process and make a new substrate or can I just resterilize before I inoculate?


--------------------
The hotter the battle, the sweeter Jah victory.
Put the heathen's back upon the wall.


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OfflinePussyFart
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Re: Beginner PF Tek, Need Reassurance [Re: 2bittoker]
    #19237502 - 12/06/13 05:10 PM (10 years, 1 month ago)

Quote:

2bittoker said:
Wipe after doing dry verm barrier.  The point of wiping is to make sure no particles prevent the lid from creating a seal, opening a vector for contams.




Wrong.

We wipe the inside 1/2" rim BEFORE applying the dry verm layer so contaminants cannot grow up/down the sides....we are only making sure it is dry.

Oh, and no tape is needed....the dry verm layer is your barrier.....duck tape will seal the jar and create a vacuum....which is bad.

Quote:

2bittoker said:
If you are doing the steam bath method, do not make and sanitize the jar until you are ready to inoculate. Steam bathing is different than pressure cooking in that the temperatures needed for autoclaving something cannot be reached in a boiling pot due to thermal energy release in steam. Steam sanitation only zaps most of the microbials and stuns the rest, long enough for the mushroom mycelium to overtake the jar.
Autoclaving with a pressure cooker at 15PSI is the only way to achieve sufficient enough sterility for short term storage of BRF cakes.



This is bullshit.

Steaming the jars sterilizes them, it does not sanitize them....dead is dead...whether accomplished with steam or pressure.

We usually recommend steaming cakes for 90 minutes minimum tho...not 60.

Quote:

2bittoker said:
I would never trust a steam sanitized jar that wasnt knocked up immediately.  You've shortened the window that the mycelium has to colonize before the contams that werent killed by the steam recover and begin colonizing.



Again, you cannot kill anything deader than dead.

This is not a race against contams....if that is the case then you have already lost, as most contams are much faster than our mycellium.

You can steam sterilize a cake for 90 minutes now, and knock it up in a month with total success.


Edited by PussyFart (12/06/13 05:11 PM)


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OfflinePussyFart
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Re: Beginner PF Tek, Need Reassurance [Re: LittleDaddy]
    #19237511 - 12/06/13 05:12 PM (10 years, 1 month ago)

Quote:

LittleDaddy said:
What is SFGC? I'm new to all these acronyms lol
Next, do I have to restart the process and make a new substrate or can I just resterilize before I inoculate?



SGFC = Shotgun Fruiting Chamber

http://www.mushroomvideos.com/Terrarium-Tek

I would sterilize them for a full 90 minutes now, and knock them up when you get time.

There is no time limit....within reason.


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Offline1down5up
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Re: Beginner PF Tek, Need Reassurance [Re: PussyFart]
    #19240732 - 12/07/13 12:41 PM (10 years, 1 month ago)

you should read the tek, and follow it line for line, don't change anything...and if you don't have everything you need, get it before you try.  As a noob, it's hard enough getting it to work when you follow the tek to a "T", it's a lot harder if you change anything...


--------------------
~~Everything is relative~~


A Simplified Overview of Mushroom Cultivation Strategies  -  RR says  -  EvilMushroom666's Take on BRF Cakes  -  Frank's list of goodies  -  Cronicr's Goodies


No one is placed higher than another no matter race or creed or gender, we must teach forgiveness and compassion for all life.  J.L.


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OfflineLittleDaddy
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Re: Beginner PF Tek, Need Reassurance [Re: 1down5up]
    #19240751 - 12/07/13 12:45 PM (10 years, 1 month ago)

I do read the tek and I know you should follow it line-for-line, but you can't expect the instructions to make perfect sense the first time someone reads it - some pages required me to reread numerous times over and even then there is still some vagueness. To an experienced user, it'll make perfect sense. To the novice, not so much.


--------------------
The hotter the battle, the sweeter Jah victory.
Put the heathen's back upon the wall.


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Offline1down5up
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Re: Beginner PF Tek, Need Reassurance [Re: LittleDaddy]
    #19241006 - 12/07/13 01:42 PM (10 years, 1 month ago)

watch the video, it's a lot clearer, and very easy to follow.  In the video they show the verm in the bowl, then they add the water and mix well, then the BRF at the end...it just mixes easier that way. 

I wasn't tryin to shit on ya LittleDaddy...just trying to stress that you should follow it as closely as you can.



there are 4 parts, just watch'em, and you should have much better luck next time around...good luck

just realized the link opens in this page, and not youtube...just search the title on YT and you should get'm all


--------------------
~~Everything is relative~~


A Simplified Overview of Mushroom Cultivation Strategies  -  RR says  -  EvilMushroom666's Take on BRF Cakes  -  Frank's list of goodies  -  Cronicr's Goodies


No one is placed higher than another no matter race or creed or gender, we must teach forgiveness and compassion for all life.  J.L.


Edited by 1down5up (12/07/13 01:43 PM)


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OfflineLittleDaddy
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Re: Beginner PF Tek, Need Reassurance [Re: 1down5up]
    #19241503 - 12/07/13 03:32 PM (10 years, 1 month ago)

Thanks for the recommendation on the video, I just watched them last night. I'm re sterilizing with no tape and am getting some micropore tape, so everything should be good for inoculation. I appreciate the advice.


--------------------
The hotter the battle, the sweeter Jah victory.
Put the heathen's back upon the wall.


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Offline1down5up
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Re: Beginner PF Tek, Need Reassurance [Re: LittleDaddy]
    #19244508 - 12/08/13 11:14 AM (10 years, 1 month ago)

no worries man...like i said, i'm a noob myself.  I've watched them 5 or 6 times now....they're stellar teaching aids.

Keep us posted on how it goes!!


--------------------
~~Everything is relative~~


A Simplified Overview of Mushroom Cultivation Strategies  -  RR says  -  EvilMushroom666's Take on BRF Cakes  -  Frank's list of goodies  -  Cronicr's Goodies


No one is placed higher than another no matter race or creed or gender, we must teach forgiveness and compassion for all life.  J.L.


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Offlinehappygolucky
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Re: Beginner PF Tek, Need Reassurance [Re: 1down5up]
    #19244926 - 12/08/13 01:08 PM (10 years, 1 month ago)

I ran an air filter called an intelli-pro for a couple hours in my room before inoculation. I then used a glove box to inoculate in. My first jar to fully colonize did so 20 days after first signs of germination. I have had no contaminants. I birthed my first cake ever, which reeked of mushroom, and spawned that bitch to bulk. Wish me luck. And I will do the same for you.


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Offlinehappygolucky
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Re: Beginner PF Tek, Need Reassurance [Re: happygolucky]
    #19245215 - 12/08/13 02:07 PM (10 years, 1 month ago)

This might help you out. It took me a few weeks to understand these things.

Spawning to bulk- This is what I was confusing with casing. Spawning to bulk is when a cake is crumbled or shredded and mixed with a bulk substrate, the most commonly used being coir and vermiculite, and put in a tray to finish colonizing then placed into fruiting, or placed in a monotub.

Casing- This is when a non-nutritive layer is used to cover the mycelial structure, such as vermiculite. Coir has nutrition in it, so would not be used as a casing material because you don't won't the casing to be colonized. It acts as a contamination barrier and like mulch. Cubes do not need to be cased.

Monotub- This is a large plastic bin that has large holes cut out of it and filled with polyfill. A piece of black plastic is placed in the bottom. Spawn is then placed in the tub with bulk substrate. The black plastic, from what I understand, acts to prevent water droplets from going down the sides onto the mycelium and creating pinning on the edges. This is a more automated setup than a sgfc.

Spawn- This is used for bulk substrates. Brf cakes can be used, but grain and wbs (wild bird seed) are more commonly used.

Bulk Substrate- This is mixed with spawn and used for larger grows than what can be achieved with cakes.


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Re: Beginner PF Tek, Need Reassurance [Re: happygolucky]
    #19254197 - 12/10/13 07:30 AM (10 years, 1 month ago)

So, in lieu of the excessive snowfall - I haven't received my syringe. I have until January 6th to be able to cultivate these mushrooms because I don't want to have them in the dorm and it'll probably be around freezing outside, so my car is out of the question (as far as I know).

I originally planned to inoculate here about a week ago and then be able to birth it about two weeks into break, but that can't happen anymore. Assuming I get the package today, would 27 days be enough or should I just store the syringe and give up on it until the weather gets good and I can keep it in my car?


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The hotter the battle, the sweeter Jah victory.
Put the heathen's back upon the wall.


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Re: Beginner PF Tek, Need Reassurance [Re: LittleDaddy]
    #19254451 - 12/10/13 09:17 AM (10 years, 1 month ago)

27 days you might have enough time to colonize if things wents perfect


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Re: Beginner PF Tek, Need Reassurance [Re: cronicr]
    #19254459 - 12/10/13 09:20 AM (10 years, 1 month ago)

hmmm... is that worth a $20 syringe? I suppose I'd have all the materials for the next cultivation besides the syringe.


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The hotter the battle, the sweeter Jah victory.
Put the heathen's back upon the wall.


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Re: Beginner PF Tek, Need Reassurance [Re: LittleDaddy]
    #19254566 - 12/10/13 09:52 AM (10 years, 1 month ago)

no it probly isn't worth taking to your dorm and getting kicked out for a few fruits


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Re: Beginner PF Tek, Need Reassurance [Re: cronicr]
    #19254576 - 12/10/13 09:57 AM (10 years, 1 month ago)

lol, that's what I was thinking. well, my long awaited first shroom trip will have to be postponed again. at least it only gives way to a better experience when it does happen.

cheers. thanks for all the contributions.


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The hotter the battle, the sweeter Jah victory.
Put the heathen's back upon the wall.


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Re: Beginner PF Tek, Need Reassurance [Re: LittleDaddy]
    #19254587 - 12/10/13 10:00 AM (10 years, 1 month ago)

shoulda done some truffles:wink:


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I'm tired do me a favor


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Re: Beginner PF Tek, Need Reassurance [Re: cronicr]
    #19254595 - 12/10/13 10:03 AM (10 years, 1 month ago)

... and the new research begins :wink:


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The hotter the battle, the sweeter Jah victory.
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Re: Beginner PF Tek, Need Reassurance [Re: LittleDaddy]
    #19257294 - 12/10/13 07:43 PM (10 years, 1 month ago)

Like they said ^^^^^^ only put tape over the holes if you use 3M micro-pore tape, because it can breathe for gas exchange and most people consider it optional as the dry verm lake makes a pretty good filter.

Next time you should only sterilize them when you know you'll be able to knock them up within the next 8-24 hours. They're slowly losing sterilization, changing moisture content and giving possible contams a chance to spread ahead of your myc.

If it's your only spore syringe I wouldn't use it on those jars. I sterilize new BRF cakes and noc them up as soon after they are cool as possible - minimum 8 hours later. Just my opinion though, they may still be fine, but if you're new you want to set yourself up with the best possible chances. There are so many other things that could go wrong. It would suck to noc up already contamed jars.:mushroom2:


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Re: Beginner PF Tek, Need Reassurance [Re: vikingsc]
    #19257322 - 12/10/13 07:48 PM (10 years, 1 month ago)

Follow this word for word and you won't have any problems:



Many people skip using a glovebox for PFTek.


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Offlinehappygolucky
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Re: Beginner PF Tek, Need Reassurance [Re: vikingsc]
    #19257346 - 12/10/13 07:53 PM (10 years, 1 month ago)

A glovebox can only increase your chances of success.


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Re: Beginner PF Tek, Need Reassurance [Re: happygolucky]
    #19257352 - 12/10/13 07:55 PM (10 years, 1 month ago)

a sab is better IMO


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It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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Re: Beginner PF Tek, Need Reassurance [Re: happygolucky]
    #19257362 - 12/10/13 07:57 PM (10 years, 1 month ago)

Quote:

happygolucky said:
A glovebox can only increase your chances of success.



A SAB is better for this hobby than a GB because the gloves are attached to you and not the box.

Making it possible to flame sterilize your tool outside the box between transfers.

Also when people try to "seal" their GBs, the spots that they missed(like around the lid) will constantly suck air in and out with your movements.

This will negate the "still air environment"....build a SAB.


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