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OfflinePsilocyBen17
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Spore print on Agar
    #19236557 - 12/06/13 01:22 PM (10 years, 1 month ago)

Hey guys,

I've done some reading on Agar and recently recieved 5 plates from The Mushroom Kingdom. I'm currently growing B+ and don't have any MS left. Instead of making a spore print and making that into a spore syringe (extra steps, meaning extra chance of contams) I was wondering if I could drop a fresh, sterile cap directly on the agar and wait for the spores to drop. Any advice if I choose to do this? I've also read that I could place a cap directly into a jar of substrate and achieve the same result.

Thanks
PsilocyBen17


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Offlineshopdropper
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Re: Spore print on Agar [Re: PsilocyBen17]
    #19236607 - 12/06/13 01:34 PM (10 years, 1 month ago)

the cap, no matter how clean you think it is, is NOT sterile. just make a nice clean print on foil or paper and scrap those spores onto the agar in a still air box. save the other plates incase you have to out run some contams.

good luck


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OfflinePsilocyBen17
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Re: Spore print on Agar [Re: shopdropper]
    #19236619 - 12/06/13 01:35 PM (10 years, 1 month ago)

Quote:

shopdropper said:
the cap, no matter how clean you think it is, is NOT sterile. just make a nice clean print on foil or paper and scrap those spores onto the agar in a still air box. save the other plates incase you have to out run some contams.

good luck




Thanks shop. I never thought of a print of foil. That will save me some steps.


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Offlinefatchillin
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Re: Spore print on Agar [Re: PsilocyBen17]
    #19237237 - 12/06/13 04:11 PM (10 years, 1 month ago)

Or you could take a fresh fruit you have, and clone a piece of the inside of the stem onto agar. Then you can clone a cluster or big fruit you'd like to have. MS is ok, but isolating is where it's at :P have fun with it :heart:


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OfflineAmanita virosa
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Re: Spore print on Agar [Re: fatchillin]
    #19239144 - 12/07/13 12:03 AM (10 years, 1 month ago)

Quote:

fatchillin said:
Or you could take a fresh fruit you have, and clone a piece of the inside of the stem onto agar. Then you can clone a cluster or big fruit you'd like to have. MS is ok, but isolating is where it's at :P have fun with it :heart:



Exactly.  Find your best fruit and clone it. Remenber to wipe it down with a bit of Iso and then tear it open with gloved hands in an sab. Never cut it open. Cutting drags surface bacteria into the inner flesh. Then use a flamed and very sharp scalpel to take a tiny piece of upper stem tissue and drop it on agar. Waa laa!


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InvisibleMudaFuka
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Re: Spore print on Agar [Re: Amanita virosa]
    #19276040 - 12/14/13 06:50 PM (10 years, 1 month ago)

I would definitely take a couple of clones but it allso never hurts to have a few prints around for when your mycelium gets to old and worn out.


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OfflineoONoodlesOo
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Re: Spore print on Agar [Re: PsilocyBen17]
    #19400907 - 01/10/14 07:59 PM (10 years, 20 days ago)

Quote:

PsilocyBen17 said:
Quote:

shopdropper said:
the cap, no matter how clean you think it is, is NOT sterile. just make a nice clean print on foil or paper and scrap those spores onto the agar in a still air box. save the other plates incase you have to out run some contams.

good luck




Thanks shop. I never thought of a print of foil. That will save me some steps.



(<.<") here. http://www.shroomery.org/11342/Spore-Prints-and-Syringes


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Offlinethemckid
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Re: Spore print on Agar [Re: oONoodlesOo]
    #19400996 - 01/10/14 08:23 PM (10 years, 20 days ago)

IMO one of the best ways to do agar work is from pins!


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OfflinePsilocyBen17
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Re: Spore print on Agar [Re: themckid]
    #19403499 - 01/11/14 12:02 PM (10 years, 19 days ago)

So you mean do a biopsy from a pin? New to agar here.


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OfflineAmanita virosa
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Re: Spore print on Agar [Re: PsilocyBen17]
    #19403811 - 01/11/14 01:12 PM (10 years, 19 days ago)

yes he means using a mushroom primordia rather than a mature mushroom for the tissue culture.  They are often cleaner.  Just remember to tear not cut the fruit you are going to clone


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OfflinePsilocyBen17
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Re: Spore print on Agar [Re: Amanita virosa]
    #19403830 - 01/11/14 01:17 PM (10 years, 19 days ago)

How would i tear a pin open? Its so tiny. Sorry for the newb questions just dont want to waste expensive agar plates


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OfflineAmanita virosa
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Re: Spore print on Agar [Re: PsilocyBen17]
    #19403849 - 01/11/14 01:20 PM (10 years, 19 days ago)

well... cut it at the base and tear it in two by grabbing either side of the cut edge.  let it get big enough to handle  somewhere between pin and fruit


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InvisibleAleon
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Re: Spore print on Agar [Re: PsilocyBen17]
    #19407102 - 01/12/14 06:43 AM (10 years, 19 days ago)

Quote:

PsilocyBen17 said:
How would i tear a pin open? Its so tiny. Sorry for the newb questions just dont want to waste expensive agar plates




Using your fingers.


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OfflineKizzle
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Re: Spore print on Agar [Re: Aleon]
    #19410400 - 01/12/14 10:12 PM (10 years, 18 days ago)

You don't need to tear open the pin when you're cloning a tiny pin like that. You can just place it on the agar and make another transfer shortly after from the outer edge of the fresh mycelium growing across the agar. That should be free of contaminants and result in a clean culture.


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InvisibleAleon
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Re: Spore print on Agar [Re: Kizzle]
    #19411359 - 01/13/14 06:15 AM (10 years, 18 days ago)

Quote:

Kizzle said:
You don't need to tear open the pin when you're cloning a tiny pin like that. You can just place it on the agar and make another transfer shortly after from the outer edge of the fresh mycelium growing across the agar. That should be free of contaminants and result in a clean culture.




That also works; i have had to do it for cloning thin or woody species like turkey tail.  I just placed a piece of the mushrooms on agar.


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Offlinesupernewfie
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Re: Spore print on Agar [Re: Kizzle]
    #19428907 - 01/16/14 04:06 PM (10 years, 14 days ago)



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Offlinesupernewfie
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Re: Spore print on Agar [Re: supernewfie]
    #19428926 - 01/16/14 04:09 PM (10 years, 14 days ago)

Ive used an old syringe for all my agar/pin cuttings.. flame sterilize the needle and cut out the agar slice, or if it is a pin i plucked the pin up by placing the top of the needle under the base and wedge it up. I pierced the base of the pin to transfer it into the agar, in my case pancake mix.


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OfflinePsilocyBen17
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Re: Spore print on Agar [Re: supernewfie]
    #19430855 - 01/16/14 10:26 PM (10 years, 14 days ago)

Woa woa woah...you made agar from pancake mix?!


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Offlinesupernewfie
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Re: Spore print on Agar [Re: PsilocyBen17]
    #19432227 - 01/17/14 08:39 AM (10 years, 14 days ago)

Yes never had agar. Cronicr has a good thread about using BRF, me not having agar or brf, and I already knew the pancake mix worked since I made a cake and it colonized quickly, I used it and as you can see, its working.



This pancake mix is whole grain and organic ... it aint no aunt jamimas lol


Edited by supernewfie (01/17/14 08:41 AM)


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OfflinePsilocyBen17
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Re: Spore print on Agar [Re: supernewfie]
    #19443730 - 01/19/14 05:32 PM (10 years, 11 days ago)

So the Pancake mix is your substrate...not your agar...


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OfflinePsilocyBen17
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Re: Spore print on Agar [Re: supernewfie]
    #19443740 - 01/19/14 05:36 PM (10 years, 11 days ago)

Im confused by what your saying. Can you post the article?


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Offlinesupernewfie
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Re: Spore print on Agar [Re: PsilocyBen17]
    #19447301 - 01/20/14 12:01 PM (10 years, 10 days ago)

Im using it to grow out mycelium from pins from agar plates, because I havent got agar. Cronicr has a thread where he used BRF.. it worked for him .. I never had brf either but I do now so il try some of that and see what happens. Il transfer the mycelium to a grain jar or something when it grows out


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Re: Spore print on Agar [Re: supernewfie]
    #19447308 - 01/20/14 12:02 PM (10 years, 10 days ago)



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Re: Spore print on Agar [Re: supernewfie]
    #19447348 - 01/20/14 12:10 PM (10 years, 10 days ago)






.


.


.


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Re: Spore print on Agar *DELETED* [Re: supernewfie]
    #19462301 - 01/23/14 12:17 PM (10 years, 7 days ago)

Post deleted by stevo

Reason for deletion: .


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OfflineRabbit88
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Re: Spore print on Agar [Re: stevo]
    #19504576 - 02/01/14 03:03 AM (9 years, 11 months ago)

Thats crazy ive never once even thought of using pancake batter as agar type substitute I guess u must adapt and use the resources around you

have you ever not added enough water and made bread in a mason jar while pressure cookin it?


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Re: Spore print on Agar [Re: Rabbit88]
    #19505581 - 02/01/14 10:32 AM (9 years, 11 months ago)





I put so much water that it never hardened its still pretty much liquid. Also the pancake mix is organic and uses a number of grains thats used in growing mushrooms.

Also I dont have a pressure cooker, I use a pot and lid to sterilize


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OfflinePsilocyBen17
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Re: Spore print on Agar [Re: supernewfie]
    #19505591 - 02/01/14 10:34 AM (9 years, 11 months ago)

I wasn't able to find the info in the thread you've posted :frown:

Do you just take organic pancake mix, add water, sterilize it in jars, and put some myc or a pin in  there after it cools?

Do you use anything as a filter?


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Offlinesupernewfie
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Re: Spore print on Agar [Re: PsilocyBen17]
    #19505606 - 02/01/14 10:37 AM (9 years, 11 months ago)

The thread im talking about is called my 50 dollar adventure, cronicr posted. And yeah water, pancake mix stir it up good make sure its not too thick caise it will turn to pancake when sterilizing if theres not enough water in it.

I dont use a filter.

BRF was used originally by cronicr


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Re: Spore print on Agar [Re: supernewfie]
    #19505632 - 02/01/14 10:44 AM (9 years, 11 months ago)

The thread I posted, page 1, cronicr explains how he does it. I suggest you follow this technique and use brf. I done this without recording weight of mix and amount of water so I wouldnt be able to tell you what to mix but in his post he gives a good explanation of how to set this kind of experiment up.


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OfflinePsilocyBen17
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Re: Spore print on Agar [Re: supernewfie]
    #19505678 - 02/01/14 10:55 AM (9 years, 11 months ago)

Ah I see. He just uses a BRF/water mix as a substitute for agar. How long would you recommend steam sterilizing for? Thanks for your help


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Re: Spore print on Agar [Re: PsilocyBen17]
    #19507932 - 02/01/14 08:10 PM (9 years, 11 months ago)

I sterilized for about 45 minutes to an hour. I checked it a few times to make sure it never solidified and added boiled water from the kettle when it did. I was just moreover fucking with it, experimenting... I used plastic microwavable containers.


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