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Stuey1
We're all mad Here



Registered: 11/28/13
Posts: 740
Loc: Bat Country
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Inoculating question
#19235523 - 12/06/13 08:34 AM (10 years, 1 month ago) |
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I am trying to inoculate 24 quart jars with a 10cc syringe. Now I know I have to heat sterilize after every jar and I've usually put a few drops through the needle to cool it down. But now I'm in a situation where every drop counts. Can I put a jar of ISO alchahol inside my SAB to dip the needle in to cool it down. I'm aware that alchahol is not sterile. But I was hoping to fill the jar with alchahol inside my SAB to help reduce anything contaminating it
-------------------- Have you ever danced with the devil in the pale moon light?
 
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PussyFart
Retired Cultivation Extrodinaire



Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
Last seen: 17 days, 15 hours
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Re: Inoculating question [Re: Stuey1]
#19235572 - 12/06/13 08:57 AM (10 years, 1 month ago) |
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Alcohol does not sterilize, it only sanitizes.....heat sterilizes.
You only need a few drops per jar.....I could make a 10cc syringe last thru 100+ jars if needed.
You are right....every drop does count.....spores are potent....stretch that shit son!
Dipping the needle in a unsterile substance will do nothing to prevent cross contamination....which is the main reason why we flame sterilize the needle between jars.....you might as well be dipping it in water.
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THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT! A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD, SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List, EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE *** *** AFGHAN KUSH GROW LOG *** ***
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Stuey1
We're all mad Here



Registered: 11/28/13
Posts: 740
Loc: Bat Country
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Re: Inoculating question [Re: PussyFart]
#19235618 - 12/06/13 09:18 AM (10 years, 1 month ago) |
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Ok. I'm not so worried then if i can use about 5 drops per quart I should be fine
-------------------- Have you ever danced with the devil in the pale moon light?
 
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bodhisatta 
Smurf real estate agent



Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Inoculating question [Re: Stuey1]
#19235808 - 12/06/13 10:30 AM (10 years, 1 month ago) |
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Quote:
Stuey1 said: Ok. I'm not so worried then if i can use about 5 drops per quart I should be fine
yep, or you can look into agar and use 5 drops of spore syringe for the rest of your life.
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Stuey1
We're all mad Here



Registered: 11/28/13
Posts: 740
Loc: Bat Country
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Re: Inoculating question [Re: bodhisatta]
#19235901 - 12/06/13 10:47 AM (10 years, 1 month ago) |
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I was gonna get my hands into some agar work when I get some prints off my first flush. Pretty excited about it. It's a whole new thing for me
-------------------- Have you ever danced with the devil in the pale moon light?
 
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bodhisatta 
Smurf real estate agent



Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Inoculating question [Re: Stuey1]
#19235923 - 12/06/13 10:52 AM (10 years, 1 month ago) |
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Quote:
Stuey1 said: I was gonna get my hands into some agar work when I get some prints off my first flush. Pretty excited about it. It's a whole new thing for me
It's easier than it seems
 besides everyone here is ready to help you out..
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Stuey1
We're all mad Here



Registered: 11/28/13
Posts: 740
Loc: Bat Country
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Re: Inoculating question [Re: bodhisatta]
#19235992 - 12/06/13 11:07 AM (10 years, 1 month ago) |
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I have faith in that for sure. Everyone's been a huge help!!!!
-------------------- Have you ever danced with the devil in the pale moon light?
 
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