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InvisibleStuey1
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Inoculating question
    #19235523 - 12/06/13 08:34 AM (10 years, 1 month ago)

I am trying to inoculate 24 quart jars with a 10cc syringe. Now I know I have to heat sterilize after every jar and I've usually put a few drops through the needle to cool it down. But now I'm in a situation where every drop counts. Can I put a jar of ISO alchahol inside my SAB to dip the needle in to cool it down. I'm aware that alchahol is not sterile. But I was hoping to fill the jar with alchahol inside my SAB to help reduce anything contaminating it


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OfflinePussyFart
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Re: Inoculating question [Re: Stuey1]
    #19235572 - 12/06/13 08:57 AM (10 years, 1 month ago)

Alcohol does not sterilize, it only sanitizes.....heat sterilizes.

You only need a few drops per jar.....I could make a 10cc syringe last thru 100+ jars if needed.

You are right....every drop does count.....spores are potent....stretch that shit son!

Dipping the needle in a unsterile substance will do nothing to prevent cross contamination....which is the main reason why we flame sterilize the needle between jars.....you might as well be dipping it in water.


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InvisibleStuey1
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Re: Inoculating question [Re: PussyFart]
    #19235618 - 12/06/13 09:18 AM (10 years, 1 month ago)

Ok. I'm not so worried then if i can use about 5 drops per quart I should be fine


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InvisiblebodhisattaMDiscordReddit
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Re: Inoculating question [Re: Stuey1]
    #19235808 - 12/06/13 10:30 AM (10 years, 1 month ago)

Quote:

Stuey1 said:
Ok. I'm not so worried then if i can use about 5 drops per quart I should be fine




yep, or you can look into agar and use 5 drops of spore syringe for the rest of your life.


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InvisibleStuey1
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Re: Inoculating question [Re: bodhisatta]
    #19235901 - 12/06/13 10:47 AM (10 years, 1 month ago)

I was gonna get my hands into some agar work when I get some prints off my first flush. Pretty excited about it. It's a whole new thing for me


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InvisiblebodhisattaMDiscordReddit
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Re: Inoculating question [Re: Stuey1]
    #19235923 - 12/06/13 10:52 AM (10 years, 1 month ago)

Quote:

Stuey1 said:
I was gonna get my hands into some agar work when I get some prints off my first flush. Pretty excited about it. It's a whole new thing for me




It's easier than it seems
:thumbup:
besides everyone here is ready to help you out..


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InvisibleStuey1
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Re: Inoculating question [Re: bodhisatta]
    #19235992 - 12/06/13 11:07 AM (10 years, 1 month ago)

I have faith in that for sure. Everyone's been a huge help!!!!


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