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Insider
Just a random platypus


Registered: 10/18/13
Posts: 56
Loc: Under the sea! Under the ...
Last seen: 9 years, 2 months
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Help... again. Bad smell.
#19233727 - 12/05/13 08:18 PM (10 years, 1 month ago) |
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Alright alright, I know everyone has told me to follow my TEK to the very T. And I will, but haven't yet got the exact ingredients so I am just experimenting to see what the heck is wrong with my entire procedure. And I believe it might be some invisible contamination. Now, for this experiment I did NOT follow the tek because of the aformentioned reasons but I beg you to bear with me.
What I did, was the following:
1- I got 3 big transparent jars, like the ones restaurants use to keep sugar and filled the holes with polyfill. 2- I made some of the verm-rye flour mix with the 2:1:1 ratio and filled the jars almost to half. 3- Steam sterilized for an hour. 4- I inoculated with agar wedges (after isolating from contams).
That was 4 days ago. I did everything as clean as possible, inside a SAB.
Now, last time I posted some pics of half pint jars I did with the same procedure but with PF tek(note: half pints had a dry verm layer, the ones I am experimenting with don't). I was told here that cold was stalling them so I took them to a warmer place and left them alone for some days to see if they restarted growth. They didn't, so naturally I opened them to take a sniff.
The smell is not easy to describe, it is kind of soury (bacterial right?) but not overwhelming and it dissapears very very fast. Visually there was no sign of contamination but I decided to throw them away.
Now the same smell is emanating from the experimental jars. I kept them without a verm layer because I wanted to be able to shake them and because I wanted to be able to see them very very clearly.
What do you think guys? Where are things going south? (Please don't say it's the ingredients, I'm confident rye flour doesn't carry more contams than BRF does).
I bought a PC by the way. 80kpa (something around 12psi). Will that help?
Thanks
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Stool_Sample
Fruity



Registered: 09/25/12
Posts: 223
Loc: North East, US
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Re: Help... again. Bad smell. [Re: Insider]
#19233748 - 12/05/13 08:26 PM (10 years, 1 month ago) |
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Quote:
Insider said: 2- I made some of the verm-rye flour mix with the 2:1:1 ratio and filled the jars almost to half. 3- Steam sterilized for an hour. 4- I inoculated with agar wedges (after isolating from contams).
That was 4 days ago. I did everything as clean as possible, inside a SAB.
I use a PC I don't steam, So look up Tek on Steaming, However I believe 90 minutes is the Recommended time for Steaming (same as PC'ing).
Why did you use the BRF tek with Agar?, The top layer of the BRF tek is an isolation area to keep the bacteria out of the Mycelium. Pic's of your JAR?
If your going to Do agar work, Just make Grain jars and Do Agar to Grain, shake Grain to more Grain. so on.
It seems like your mixing Tek's together and Failing. Just my guess.
-------------------- "wise words here"
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Insider
Just a random platypus


Registered: 10/18/13
Posts: 56
Loc: Under the sea! Under the ...
Last seen: 9 years, 2 months
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Yes, I mixed both teks just to experiment while I get some BRF. What puzzles me is that in any case I get the same weird smell and I want to know if it is because of the "sterilization time" or because something completely different.
Last time I did PF tek normally except I used rye flour instead of BRF and had the same smell.
Edited by Insider (12/05/13 08:30 PM)
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Stool_Sample
Fruity



Registered: 09/25/12
Posts: 223
Loc: North East, US
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Re: Help... again. Bad smell. [Re: Insider]
#19233764 - 12/05/13 08:30 PM (10 years, 1 month ago) |
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Do your jars have holes ontop, or do your jars have filters ontop?
-------------------- "wise words here"
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Insider
Just a random platypus


Registered: 10/18/13
Posts: 56
Loc: Under the sea! Under the ...
Last seen: 9 years, 2 months
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Polyfill.
I was adviced to remove the polyfill from the half pints because that was what verm layer is for, so I did. My current vermless jars have polyfill on top.
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Stool_Sample
Fruity



Registered: 09/25/12
Posts: 223
Loc: North East, US
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Re: Help... again. Bad smell. [Re: Insider]
#19233784 - 12/05/13 08:35 PM (10 years, 1 month ago) |
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well, if you take off the Filter, then throw in a agar wedge. That shit will contam.
You cannot bypass a Verm barrier with the agar and think it's going to be clean.
Just as you can not expect a Jar without a filter to remain clean.
Pic a Tek, Follow it. Good luck.
-------------------- "wise words here"
Edited by Stool_Sample (12/05/13 08:36 PM)
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Insider
Just a random platypus


Registered: 10/18/13
Posts: 56
Loc: Under the sea! Under the ...
Last seen: 9 years, 2 months
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Quote:
Stool_Sample said: well, if you take off the Filter, then throw in a agar wedge. That shit will contam.
You cannot bypass a Verm barrier with the agar and think it's going to be clean.
Just as you can not expect a Jar without a filter to remain clean.
Pic a Tek, Follow it. Good luck.
Thanks, will it contam even if done every step inside a SAB?
Edit: Wait, no no no. I didn“t explain myself properly. Half pints were (because they no longer exist) inoculated with a spore syringe. Current vermless jars were inoculated with agar wedges.
Edited by Insider (12/05/13 08:39 PM)
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PussyFart
Retired Cultivation Extrodinaire



Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
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Quote:
Stool_Sample said: well, if you take off the Filter, then throw in a agar wedge. That shit will contam.
Not if done in a SAB
--------------------
THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT! A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD, SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List, EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE *** *** AFGHAN KUSH GROW LOG *** ***
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Insider
Just a random platypus


Registered: 10/18/13
Posts: 56
Loc: Under the sea! Under the ...
Last seen: 9 years, 2 months
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Re: Help... again. Bad smell. [Re: PussyFart]
#19233851 - 12/05/13 08:49 PM (10 years, 1 month ago) |
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So in the end it is contamination right? Bacterial contamination? Will the PC help in this matter? For how long should I PC? 90 minutes?
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Stool_Sample
Fruity



Registered: 09/25/12
Posts: 223
Loc: North East, US
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Re: Help... again. Bad smell. [Re: PussyFart]
#19234166 - 12/05/13 09:58 PM (10 years, 1 month ago) |
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Quote:
Notahacker420 said:
Quote:
Stool_Sample said: well, if you take off the Filter, then throw in a agar wedge. That shit will contam.
Not if done in a SAB
Once the Filter is gone....SAB won't matter.
-------------------- "wise words here"
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Insider
Just a random platypus


Registered: 10/18/13
Posts: 56
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Last seen: 9 years, 2 months
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What? No, see: I first prepare the jars, then steam sterilize with the lid and the polyfill in. Then INSIDE the SAB remove the LID and drop the agar wedge, then put the lid in again, tight.
The polyfill is never removed on these ones (vermless).
I removed the polyfill of the OTHER jars. The half pint with verm ones, to provide GE.
I am talking about two different kinds of jars:
-Normal PF tek jars of a previos attempt. -Experimental vermless jars of current attempt.
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KnownPown
Fathead Shroomer


Registered: 10/27/12
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Re: Help... again. Bad smell. [Re: Insider]
#19234451 - 12/05/13 11:10 PM (10 years, 1 month ago) |
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Imo it could be the rye flour wasnt steamed for long enough, I know you can steam and sterilize brf but I'm not sure about the rye since it could have endospores like other grains? And need to be actually pressure cooked.
If you haven't had a success, I would 1. quit experimenting and gather the right equipment before you continue. 2. Pick a tek (bulk imo) and follow it.
So you cant find brf? I'm sure you can find wild birdseed, popcorn, rye berries or something for bulk right? Might as well quit wasting time with small time shit and just get to bulk so when you waste 90 minutes sterilizing it wont be for a bunch of half pints, but rather quarts...plus agar work does much better with grains anyway.
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Insider
Just a random platypus


Registered: 10/18/13
Posts: 56
Loc: Under the sea! Under the ...
Last seen: 9 years, 2 months
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Re: Help... again. Bad smell. [Re: KnownPown]
#19234466 - 12/05/13 11:15 PM (10 years, 1 month ago) |
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Roger that. Will quit experimenting, that's for sure. And yes, I will follow the damned tek to the very T. Thanks.
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