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TheMustardTiger



Registered: 08/19/13
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Syringe filters for GE?
#19232971 - 12/05/13 05:37 PM (10 years, 2 months ago) |
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Picked up these 13mm "syringe filters" that are supposed to provide contam-free gas exchange for inoculated jars. Just drill a hole on a plastic lid, pop it in and you're good to go. Anyone have any experience with these? They come in 13mm and 25mm sizes; 13mm should be plenty for healthy GE, yes?
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PussyFart
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They are good for GE on LC jars and thats about it.
They restrict too much GE for grain jars or cakes IMO.
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fractaldill
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Re: Syringe filters for GE? [Re: PussyFart]
#19233211 - 12/05/13 06:32 PM (10 years, 2 months ago) |
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Quote:
Notahacker420 said: They are good for GE on LC jars and thats about it.
They restrict too much GE for grain jars or cakes IMO.
Yep, they are perfect for liquid cultures as synthetic filter discs can sometimes allow molds to germinate and grow through them.
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clueless
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Re: Syringe filters for GE? [Re: fractaldill]
#19233296 - 12/05/13 06:47 PM (10 years, 2 months ago) |
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Quote:
fractaldill said:
Quote:
Notahacker420 said: They are good for GE on LC jars and thats about it.
They restrict too much GE for grain jars or cakes IMO.
Yep, they are perfect for liquid cultures as synthetic filter discs can sometimes allow molds to germinate and grow through them.
Do you have any examples of that?
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TheMustardTiger



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Re: Syringe filters for GE? [Re: fractaldill]
#19233310 - 12/05/13 06:49 PM (10 years, 2 months ago) |
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So you've tried them on grain jars? I was actually considering trying them out on a grain jar just to see, but it's a definite no go?
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fractaldill
Cultivator in Training

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if you're doing grain jars make your own lids. its much cheaper, and just as efficient.
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Icesyn
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I believe people are thinking of a whatman filter which is only helpful with liquid cultures really due to the small passage. Also they aren't meant to handle needles.

I haven't heard of a syringe filter nor do I understand how something can be jabbed with a needle and then still filter air efficiently. I can't imagine a healable(sealing) micropore material.
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fractaldill
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Re: Syringe filters for GE? [Re: Icesyn]
#19233405 - 12/05/13 07:11 PM (10 years, 2 months ago) |
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Its very easy to make a lid with a SHIP and syringe filter.
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TheMustardTiger



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Re: Syringe filters for GE? [Re: Icesyn]
#19233424 - 12/05/13 07:16 PM (10 years, 2 months ago) |
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Quote:
Icesyn said: I believe people are thinking of a whatman filter which is only helpful with liquid cultures really due to the small passage. Also they aren't meant to handle needles.

I haven't heard of a syringe filter nor do I understand how something can be jabbed with a needle and then still filter air efficiently. I can't imagine a healable(sealing) micropore material.
I didn't come up with the name, that's just what they're called on the website. so these are not sufficient for GE on grains?
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fractaldill
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they are. just not very practical.
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TheMustardTiger



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I use self healing injection ports when inoculating via spore syringe so I wasn't planning on shoving a needle through it. Just wondering if the hole was large enough for sufficient GE for grains. Basically, it seemed like a nifty way to get GE and I was hoping to get some people that may have actually tried this.
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CliftonGK1
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Re: Syringe filters for GE? [Re: Icesyn]
#19233445 - 12/05/13 07:21 PM (10 years, 2 months ago) |
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Quote:
Icesyn said: I believe people are thinking of a whatman filter which is only helpful with liquid cultures really due to the small passage. Also they aren't meant to handle needles.

I haven't heard of a syringe filter nor do I understand how something can be jabbed with a needle and then still filter air efficiently. I can't imagine a healable(sealing) micropore material.
Whatman, Acrodisc (Pall-Gelman) and many others out there have pore sizes that are typically too small for adequate passive gas exchange. The common pore sizes are 0.45um and 0.20um and they're used for pressure filtration of culture media or injectables. I used to use them all the time in cell culture labs. They get the generic name of "syringe filters" because they have a luer-lock and you can lock them onto a syringe to force your media through one. No, they aren't self healing. If you overload one and pop the membrane, that's it man. It's done for. Pall made the Acrodisc in some bigass sizes, like a CPC lock size for in-line filtration of bulk culture media being pumped from 200L stock barrels into bioreactors. I've used a slightly smaller size with much success for an in-line air filter on a fishtank pump I was using to automate FAE on a chamber.
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fractaldill
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yes, you can use it for GE. they are just very expensive if you want to use it for many jars.
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TheMustardTiger



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Re: Syringe filters for GE? [Re: CliftonGK1]
#19233467 - 12/05/13 07:28 PM (10 years, 2 months ago) |
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Very interesting, Thanks for detailed explanation. I do not know of the pore size for this particular model so I'm in the dark there. Even. So, what's your opinion on using this without a pump system?
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fractaldill
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HUH? dude. these are used as GE filters and nothing more. You literally drill a hole, fill it up with rtv, and shove it through. after that you dont touch it. you inoculate through a SHIP. http://www.themadseason.com/PP5-Injectable-Liquid-Culture-Lid-p/3001.htm
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Icesyn
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Re: Syringe filters for GE? [Re: fractaldill]
#19233509 - 12/05/13 07:42 PM (10 years, 2 months ago) |
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Just a suggestion, posted this earlier.
Quote:
Icesyn said:
  
EZ Felt
100% sylicone glue for inoc port and 25-cent sized stiffened felt disk glued on around the g/e hole(s).
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TheMustardTiger



Registered: 08/19/13
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Re: Syringe filters for GE? [Re: fractaldill]
#19233512 - 12/05/13 07:42 PM (10 years, 2 months ago) |
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You're telling me what I already know. I have, and use regularly, SHIP. I was wondering if anyone had used these "syringe filters" as a means for gas exchange in grain jars and whether or not it was successful. What aren't you understanding?
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TheMustardTiger



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Re: Syringe filters for GE? [Re: Icesyn]
#19233519 - 12/05/13 07:45 PM (10 years, 2 months ago) |
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Quote:
Icesyn said: Just a suggestion, posted this earlier.
Quote:
Icesyn said:
  
EZ Felt
100% sylicone glue for inoc port and 25-cent sized stiffened felt disk glued on around the g/e hole(s).
This is new to me as well. Seems like it work just fine. I may try this down the road too.
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Icesyn
Trust My Words


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Quote:
MustardTiger66 said: You're telling me what I already know. I have, and use regularly, SHIP. I was wondering if anyone had used these "syringe filters" as a means for gas exchange in grain jars and whether or not it was successful. What aren't you understanding?

Quote:
CliftonGK1 said: Whatman, Acrodisc (Pall-Gelman) and many others out there have pore sizes that are typically too small for adequate passive gas exchange. The common pore sizes are 0.45um and 0.20um and they're used for pressure filtration of culture media or injectables. I used to use them all the time in cell culture labs. They get the generic name of "syringe filters" because they have a luer-lock and you can lock them onto a syringe to force your media through one. No, they aren't self healing. If you overload one and pop the membrane, that's it man. It's done for. Pall made the Acrodisc in some bigass sizes, like a CPC lock size for in-line filtration of bulk culture media being pumped from 200L stock barrels into bioreactors. I've used a slightly smaller size with much success for an in-line air filter on a fishtank pump I was using to automate FAE on a chamber.
Interesting
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TheMustardTiger



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Re: Syringe filters for GE? [Re: Icesyn]
#19233535 - 12/05/13 07:47 PM (10 years, 2 months ago) |
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Quote:
Icesyn said:
Quote:
MustardTiger66 said: You're telling me what I already know. I have, and use regularly, SHIP. I was wondering if anyone had used these "syringe filters" as a means for gas exchange in grain jars and whether or not it was successful. What aren't you understanding?

That wasn't directed at you. should've quoted. My bad.
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