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OfflineTheMustardTiger
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Syringe filters for GE?
    #19232971 - 12/05/13 05:37 PM (10 years, 1 month ago)

Picked up these 13mm "syringe filters" that are supposed to provide contam-free gas exchange for inoculated jars. Just drill a hole on a plastic lid, pop it in and you're good to go. Anyone have any experience with these? They come in 13mm and 25mm sizes; 13mm should be plenty for healthy GE, yes?


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OfflinePussyFart
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Re: Syringe filters for GE? [Re: TheMustardTiger]
    #19233157 - 12/05/13 06:22 PM (10 years, 1 month ago)

They are good for GE on LC jars and thats about it.

They restrict too much GE for grain jars or cakes IMO.


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Offlinefractaldill
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Re: Syringe filters for GE? [Re: PussyFart]
    #19233211 - 12/05/13 06:32 PM (10 years, 1 month ago)

Quote:

Notahacker420 said:
They are good for GE on LC jars and thats about it.

They restrict too much GE for grain jars or cakes IMO.




Yep, they are perfect for liquid cultures as synthetic filter discs can sometimes allow molds to germinate and grow through them.


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Offlineclueless
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Re: Syringe filters for GE? [Re: fractaldill]
    #19233296 - 12/05/13 06:47 PM (10 years, 1 month ago)

Quote:

fractaldill said:
Quote:

Notahacker420 said:
They are good for GE on LC jars and thats about it.

They restrict too much GE for grain jars or cakes IMO.




Yep, they are perfect for liquid cultures as synthetic filter discs can sometimes allow molds to germinate and grow through them.




Do you have any examples of that?


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OfflineTheMustardTiger
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Re: Syringe filters for GE? [Re: fractaldill]
    #19233310 - 12/05/13 06:49 PM (10 years, 1 month ago)

So you've tried them on grain jars? I was actually considering trying them out on a grain jar just to see, but it's a definite no go?


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Offlinefractaldill
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Re: Syringe filters for GE? [Re: TheMustardTiger]
    #19233370 - 12/05/13 07:01 PM (10 years, 1 month ago)

if you're doing grain jars make your own lids. its much cheaper, and just as efficient.


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OfflineIcesyn
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Re: Syringe filters for GE? [Re: TheMustardTiger]
    #19233379 - 12/05/13 07:04 PM (10 years, 1 month ago)

I believe people are thinking of a whatman filter which is only helpful with liquid cultures really due to the small passage. Also they aren't meant to handle needles.


I haven't heard of a syringe filter nor do I understand how something can be jabbed with a needle and then still filter air efficiently. I can't imagine a healable(sealing) micropore material.


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Offlinefractaldill
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Re: Syringe filters for GE? [Re: Icesyn]
    #19233405 - 12/05/13 07:11 PM (10 years, 1 month ago)

Its very easy to make a lid with a SHIP and syringe filter.


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OfflineTheMustardTiger
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Re: Syringe filters for GE? [Re: Icesyn]
    #19233424 - 12/05/13 07:16 PM (10 years, 1 month ago)

Quote:

Icesyn said:
I believe people are thinking of a whatman filter which is only helpful with liquid cultures really due to the small passage. Also they aren't meant to handle needles.


I haven't heard of a syringe filter nor do I understand how something can be jabbed with a needle and then still filter air efficiently. I can't imagine a healable(sealing) micropore material.




I didn't come up with the name, that's just what they're called on the website. so these are not sufficient for GE on grains?


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Offlinefractaldill
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Re: Syringe filters for GE? [Re: TheMustardTiger]
    #19233430 - 12/05/13 07:18 PM (10 years, 1 month ago)

they are. just not very practical.


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OfflineTheMustardTiger
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Re: Syringe filters for GE? [Re: TheMustardTiger]
    #19233442 - 12/05/13 07:21 PM (10 years, 1 month ago)

I use self healing injection ports when inoculating via spore syringe so I wasn't planning on shoving a needle through it. Just wondering if the hole was large enough for sufficient GE for grains. Basically, it seemed like a nifty way to get GE and I was hoping to get some people that may have actually tried this.


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OfflineCliftonGK1
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Re: Syringe filters for GE? [Re: Icesyn]
    #19233445 - 12/05/13 07:21 PM (10 years, 1 month ago)

Quote:

Icesyn said:
I believe people are thinking of a whatman filter which is only helpful with liquid cultures really due to the small passage. Also they aren't meant to handle needles.


I haven't heard of a syringe filter nor do I understand how something can be jabbed with a needle and then still filter air efficiently. I can't imagine a healable(sealing) micropore material.




Whatman, Acrodisc (Pall-Gelman) and many others out there have pore sizes that are typically too small for adequate passive gas exchange.  The common pore sizes are 0.45um and 0.20um and they're used for pressure filtration of culture media or injectables.  I used to use them all the time in cell culture labs.
They get the generic name of "syringe filters" because they have a luer-lock and you can lock them onto a syringe to force your media through one.  No, they aren't self healing.  If you overload one and pop the membrane, that's it man.  It's done for.
Pall made the Acrodisc in some bigass sizes, like a CPC lock size for in-line filtration of bulk culture media being pumped from 200L stock barrels into bioreactors.  I've used a slightly smaller size with much success for an in-line air filter on a fishtank pump I was using to automate FAE on a chamber.


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Offlinefractaldill
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Re: Syringe filters for GE? [Re: TheMustardTiger]
    #19233456 - 12/05/13 07:25 PM (10 years, 1 month ago)

yes, you can use it for GE. they are just very expensive if you want to use it for many jars.


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OfflineTheMustardTiger
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Re: Syringe filters for GE? [Re: CliftonGK1]
    #19233467 - 12/05/13 07:28 PM (10 years, 1 month ago)

Very interesting, Thanks for detailed explanation.  I do not know of the pore size for this particular model so I'm in the dark there. Even. So, what's your opinion on using this without a pump system?


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Offlinefractaldill
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Re: Syringe filters for GE? [Re: TheMustardTiger]
    #19233482 - 12/05/13 07:33 PM (10 years, 1 month ago)

HUH? dude. these are used as GE filters and nothing more. You literally drill a hole, fill it up with rtv, and shove it through. after that you dont touch it. you inoculate through a SHIP. http://www.themadseason.com/PP5-Injectable-Liquid-Culture-Lid-p/3001.htm


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OfflineIcesyn
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Re: Syringe filters for GE? [Re: fractaldill]
    #19233509 - 12/05/13 07:42 PM (10 years, 1 month ago)

Just a suggestion, posted this earlier.
Quote:

Icesyn said:


EZ Felt

100% sylicone glue for inoc port and 25-cent sized stiffened felt disk glued on around the g/e hole(s).




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OfflineTheMustardTiger
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Re: Syringe filters for GE? [Re: fractaldill]
    #19233512 - 12/05/13 07:42 PM (10 years, 1 month ago)

You're telling me what I already know. I have, and use regularly, SHIP. I was wondering if anyone had used these "syringe filters" as a means for gas exchange in grain jars and whether or not it was successful. What aren't you understanding?


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OfflineTheMustardTiger
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Re: Syringe filters for GE? [Re: Icesyn]
    #19233519 - 12/05/13 07:45 PM (10 years, 1 month ago)

Quote:

Icesyn said:
Just a suggestion, posted this earlier.
Quote:

Icesyn said:


EZ Felt

100% sylicone glue for inoc port and 25-cent sized stiffened felt disk glued on around the g/e hole(s).







This is new to me as well. Seems like it work just fine. I may try this down the road too.


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OfflineIcesyn
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Re: Syringe filters for GE? [Re: TheMustardTiger]
    #19233522 - 12/05/13 07:45 PM (10 years, 1 month ago)

Quote:

MustardTiger66 said:
You're telling me what I already know. I have, and use regularly, SHIP. I was wondering if anyone had used these "syringe filters" as a means for gas exchange in grain jars and whether or not it was successful. What aren't you understanding?



:bitchsmack:

Quote:

CliftonGK1 said:
Whatman, Acrodisc (Pall-Gelman) and many others out there have pore sizes that are typically too small for adequate passive gas exchange.  The common pore sizes are 0.45um and 0.20um and they're used for pressure filtration of culture media or injectables.  I used to use them all the time in cell culture labs.
They get the generic name of "syringe filters" because they have a luer-lock and you can lock them onto a syringe to force your media through one.  No, they aren't self healing.  If you overload one and pop the membrane, that's it man.  It's done for.
Pall made the Acrodisc in some bigass sizes, like a CPC lock size for in-line filtration of bulk culture media being pumped from 200L stock barrels into bioreactors.  I've used a slightly smaller size with much success for an in-line air filter on a fishtank pump I was using to automate FAE on a chamber.




Interesting  :thatsinteresting:


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OfflineTheMustardTiger
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Re: Syringe filters for GE? [Re: Icesyn]
    #19233535 - 12/05/13 07:47 PM (10 years, 1 month ago)

Quote:

Icesyn said:
Quote:

MustardTiger66 said:
You're telling me what I already know. I have, and use regularly, SHIP. I was wondering if anyone had used these "syringe filters" as a means for gas exchange in grain jars and whether or not it was successful. What aren't you understanding?



:bitchsmack:



That wasn't directed at you. should've quoted. My bad.


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Offlinefractaldill
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Re: Syringe filters for GE? [Re: TheMustardTiger]
    #19233537 - 12/05/13 07:48 PM (10 years, 1 month ago)

Quote:

MustardTiger66 said:
You're telling me what I already know. I have, and use regularly, SHIP. I was wondering if anyone had used these "syringe filters" as a means for gas exchange in grain jars and whether or not it was successful. What aren't you understanding?



are you trolling or something?
YES you can use these for gas exchange, they work fine. You CANNOT inoculate through them, so you will have to find another way to do so.


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OfflineIcesyn
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Re: Syringe filters for GE? [Re: TheMustardTiger]
    #19233556 - 12/05/13 07:51 PM (10 years, 1 month ago)

Quote:

MustardTiger66 said:
That wasn't directed at you. should've quoted. My bad.



Yeah I understand.

Stay cool man :paradisiscoolwhat:


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OfflineTheMustardTiger
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Re: Syringe filters for GE? [Re: fractaldill]
    #19233569 - 12/05/13 07:54 PM (10 years, 1 month ago)

Quote:

fractaldill said:
Quote:

MustardTiger66 said:
You're telling me what I already know. I have, and use regularly, SHIP. I was wondering if anyone had used these "syringe filters" as a means for gas exchange in grain jars and whether or not it was successful. What aren't you understanding?



are you trolling or something?
YES you can use these for gas exchange, they work fine. You CANNOT inoculate through them, so you will have to find another way to do so.



Trolling? First off, I never said I wanted to inoculate with them. 2nd off, I know that you can use them for GE, that's what they're advertised for. My question, which for some strange reason you can't seem to grasp, is this: Has anyone used these things on grain jars and if you did, what the hell happened when you did it?


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Offlinefractaldill
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Re: Syringe filters for GE? [Re: TheMustardTiger]
    #19233573 - 12/05/13 07:56 PM (10 years, 1 month ago)

They work just fine for grain jars... http://out-grow.com/mushroom-growing-substrates-c-4/4-quart-jars-of-grain-spawn-with-self-healing-injectors-p-22.html
They work like any other form of GE medium. they are just very expensive.


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OfflineTheMustardTiger
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Re: Syringe filters for GE? [Re: fractaldill]
    #19233589 - 12/05/13 08:01 PM (10 years, 1 month ago)

They were a dollar and some change a piece, not breaking the bank at all; a trip to Mcdonalds is more expensive. . They are advertised as being able to provide LC jars (Explicitly) with sufficient GE. I have never seen or heard of anyone using them with quart jars of grain, hence my inquiry: Has anyone used these on grain jars and do your grains and the myc colonizing them receive adequate GE. Very simple question.


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Offlinefractaldill
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Re: Syringe filters for GE? [Re: TheMustardTiger]
    #19233591 - 12/05/13 08:03 PM (10 years, 1 month ago)

when youre working 100 jars at once, it may just make a dent.


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OfflinePussyFart
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Re: Syringe filters for GE? [Re: fractaldill]
    #19233605 - 12/05/13 08:07 PM (10 years, 1 month ago)

Quote:

TranscendingLife said:
Whatmans are not to be used on grains jars as they don't allow enough GE.
They're for LCs, because you only need GE for LC jars to ensure there is no vacuum.



http://www.shroomery.org/forums/showflat.php/Number/15852793#15852793

Quote:

TranscendingLife said:
Also, Whatman syringe filters are not suitable for grains.
They don't allow enough GE.



http://www.shroomery.org/forums/showflat.php/Number/18446350#18446350

Quote:

FooMan said:
Syringe filters should be used for liquid cultures, not grain jars. They don't provide enough gas exchange for grains, whereas LC's don't need GE. Use the discs for grain jars.



http://www.shroomery.org/forums/showflat.php/Number/18435452#18435452

Quote:

shamanamba said:
Anyway, I'm surprised that a site sponsor is selling grain jars with syringe filters for GE. They should know that those filters are notorious for restricting GE too much and causing stalled growth.

Those filters are good for LC's and air displacement on sterile water containers, but not grain jars.



http://www.shroomery.org/forums/showflat.php/Number/16783039#16783039

http://www.shroomery.org/forums/showflat.php/Number/18401615#18401615


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OfflineTheMustardTiger
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Re: Syringe filters for GE? [Re: fractaldill]
    #19233607 - 12/05/13 08:07 PM (10 years, 1 month ago)

Quote:

fractaldill said:
when youre working 100 jars at once, it may just make a dent.




Well excuse me, your highness.. I'm sorry my meager 8 jars doesn't turn up any blips on your radar..


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Offlinefractaldill
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Re: Syringe filters for GE? [Re: TheMustardTiger]
    #19233624 - 12/05/13 08:12 PM (10 years, 1 month ago)

im just saying there are much more practical ways of doing things.


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OfflineTheMustardTiger
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Re: Syringe filters for GE? [Re: PussyFart]
    #19233628 - 12/05/13 08:13 PM (10 years, 1 month ago)

Quote:

Notahacker420 said:
Quote:

TranscendingLife said:
Whatmans are not to be used on grains jars as they don't allow enough GE.
They're for LCs, because you only need GE for LC jars to ensure there is no vacuum.



http://www.shroomery.org/forums/showflat.php/Number/15852793#15852793

Quote:

TranscendingLife said:
Also, Whatman syringe filters are not suitable for grains.
They don't allow enough GE.



http://www.shroomery.org/forums/showflat.php/Number/18446350#18446350

Quote:

FooMan said:
Syringe filters should be used for liquid cultures, not grain jars. They don't provide enough gas exchange for grains, whereas LC's don't need GE. Use the discs for grain jars.



http://www.shroomery.org/forums/showflat.php/Number/18435452#18435452

Quote:

shamanamba said:
Anyway, I'm surprised that a site sponsor is selling grain jars with syringe filters for GE. They should know that those filters are notorious for restricting GE too much and causing stalled growth.

Those filters are good for LC's and air displacement on sterile water containers, but not grain jars.



http://www.shroomery.org/forums/showflat.php/Number/16783039#16783039

http://www.shroomery.org/forums/showflat.php/Number/18401615#18401615




Well that made the room a little brighter. Thanks for this, definitely cleared things up.:thumbup:


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