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Offlinetwistedty
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Re: Black mold? or some other kind of contam? (pictures included) [Re: dessidoll93]
    #19230910 - 12/05/13 12:02 PM (10 years, 2 months ago)

Quote:

dessidoll93 said:
I steamed them in a large pot. I do have a 1/2 inch verm layer. I did flame my needle, as well as wiped it with alcohol. BUT I did inoculate in open air but there wasn't any air on and I disinfected as best as I could. I'm just so sad to seem them go!





you should only flame sterilize, if you flame sterilize then wipe with anything you need to flame sterilize again.

not saying this lead to your failure that is if its contammed


Edited by twistedty (12/05/13 12:02 PM)


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Invisibleelasticaltiger
Like Tigers in Coitus
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Registered: 06/24/13
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Re: Black mold? or some other kind of contam? (pictures included) [Re: twistedty]
    #19230938 - 12/05/13 12:08 PM (10 years, 2 months ago)

Quote:

twistedty said:
Quote:

dessidoll93 said:
I steamed them in a large pot. I do have a 1/2 inch verm layer. I did flame my needle, as well as wiped it with alcohol. BUT I did inoculate in open air but there wasn't any air on and I disinfected as best as I could. I'm just so sad to seem them go!





you should only flame sterilize, if you flame sterilize then wipe with anything you need to flame sterilize again.

not saying this lead to your failure that is if its contammed




Beat me to it. Flame sterilization is all you need. Heat will kill everything. Alcohol will only kill 99.99% of contamination.


--------------------
First time growing cakes? DON'T make a Shotgun Fruiting Chamber

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No Pouring. No Syringes. No Cutting. No flaming. No Contamination. No Bullshit.

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OfflineIrfan
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Registered: 09/06/13
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Re: Black mold? or some other kind of contam? (pictures included) [Re: dessidoll93]
    #19230964 - 12/05/13 12:14 PM (10 years, 2 months ago)

Quote:

dessidoll93 said:
I steamed them in a large pot. I do have a 1/2 inch verm layer. I did flame my needle, as well as wiped it with alcohol. BUT I did inoculate in open air but there wasn't any air on and I disinfected as best as I could. I'm just so sad to seem them go!




Black molds can cause serious permanent health problems.  I am empathetic but its best to let them go...



Buy a pressure cooker.


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InvisibleJosh.0
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Posts: 553
Re: Black mold? or some other kind of contam? (pictures included) [Re: twistedty]
    #19230984 - 12/05/13 12:20 PM (10 years, 2 months ago)

Quote:

dessidoll93 said:
I'm just so sad to seem them go!



:crazybaby: think of how happy your going to be when the next set of jars pans out just as you hoped :slowreaction:


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:trippinbawelz:                              :trippinballs::mindblown::trippinballs:                      :awecid:

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OfflineKizzle
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Re: Black mold? or some other kind of contam? (pictures included) [Re: Irfan]
    #19231073 - 12/05/13 12:47 PM (10 years, 2 months ago)

I've never used any the syringes with charcoal but this is supposedly what they're supposed to look like. I'd check the site of the place got it from and see if it mentions it.



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Offline2bittoker
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Registered: 03/09/13
Posts: 555
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Re: Black mold? or some other kind of contam? (pictures included) [Re: elasticaltiger]
    #19231667 - 12/05/13 03:05 PM (10 years, 2 months ago)

Quote:

elasticaltiger said:
Quote:

twistedty said:
Quote:

dessidoll93 said:
I steamed them in a large pot. I do have a 1/2 inch verm layer. I did flame my needle, as well as wiped it with alcohol. BUT I did inoculate in open air but there wasn't any air on and I disinfected as best as I could. I'm just so sad to seem them go!





you should only flame sterilize, if you flame sterilize then wipe with anything you need to flame sterilize again.

not saying this lead to your failure that is if its contammed




Beat me to it. Flame sterilization is all you need. Heat will kill everything. Alcohol will only kill 99.99% of contamination.



Negative.  Alcohol only kills bacteria (at 70-30 mix), a SMALL amount viruses.  It doesnt do jack to Mold or mold spores, like Trich Spores. Wiping down the needle with alcohol should only be done if there's something on it that you dont want to burn, like extra verm between jars.


--------------------
“I slept and dreamt that life was joy. I awoke and saw that life was service. I acted and behold, service was joy.”
"Love does not claim possession, but gives freedom"
― Rabindranath Tagore

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Where new growers should start: RogerRabbit's PF Tek video    How it Should and Shouldn't Look 
My Simplified Bulk Growing  My OJ Shroom Tek


Edited by 2bittoker (12/05/13 03:06 PM)


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OfflinePussyFart
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Re: Black mold? or some other kind of contam? (pictures included) [Re: 2bittoker]
    #19231921 - 12/05/13 04:00 PM (10 years, 2 months ago)

Wiping a flame sterilized tool with an unsterile substance like alcohol is like wiping shit on your face after a shower....

-RR


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Offlinesharpshroomer98
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Registered: 08/30/11
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Re: Black mold? or some other kind of contam? (pictures included) [Re: PussyFart]
    #19231932 - 12/05/13 04:02 PM (10 years, 2 months ago)

Quote:

Notahacker420 said:
Wiping a flame sterilized tool with an unsterile substance like alcohol is like wiping shit on your face after a shower....

-RR






:nicesmile:

Mycology logic lol,, it's the best.


--------------------
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Offline2bittoker
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Registered: 03/09/13
Posts: 555
Last seen: 2 years, 4 months
Re: Black mold? or some other kind of contam? (pictures included) [Re: PussyFart]
    #19233027 - 12/05/13 05:51 PM (10 years, 2 months ago)

Quote:

Notahacker420 said:
Wiping a flame sterilized tool with an unsterile substance like alcohol is like wiping shit on your face after a shower....

-RR



I hope that wasnt in reply to me.  Since I was talking about cleaning off needles of debris gathered between jars, before flame sterilizing.  And needles after inoculating jars and being exposed to open air are assumed to be no longer sterile, hence flame sterlizing between jars.


--------------------
“I slept and dreamt that life was joy. I awoke and saw that life was service. I acted and behold, service was joy.”
"Love does not claim possession, but gives freedom"
― Rabindranath Tagore

Stuff for New Growers
Where new growers should start: RogerRabbit's PF Tek video    How it Should and Shouldn't Look 
My Simplified Bulk Growing  My OJ Shroom Tek


Edited by 2bittoker (12/05/13 09:30 PM)


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