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36fuckin5
Alchemycologist


Registered: 08/11/03
Posts: 12,079
Loc: Diving into Mystical Territori...
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Re: Going to start pasteurizing in bags. Oven bags vs filter patch bags? [Re: Stromrider]
#19249074 - 12/09/13 07:58 AM (10 years, 1 month ago) |
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I throw my hot substrate into my tub and mix it till it's cool enough to spawn. Mostly just to make things go quicker.
-------------------- Redd Foxx said: If you're offended I don't give a shit and don't come see me no more. Pat The Bunny said: A punk rock song won't ever change the world, but I can tell you about a couple that changed me. bodhisatta said: i recommend common sense and figuring it out. These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.
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Stromrider
This must be the place



Registered: 06/02/13
Posts: 7,326
Loc: Dept of know what I'm say...
Last seen: 39 minutes, 8 seconds
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Re: Going to start pasteurizing in bags. Oven bags vs filter patch bags? [Re: 36fuckin5]
#19249091 - 12/09/13 08:06 AM (10 years, 1 month ago) |
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Yeah I've done that as well
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Generic
Registered: 11/12/13
Posts: 176
Loc: Oregon
Last seen: 1 day, 19 hours
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Re: Going to start pasteurizing in bags. Oven bags vs filter patch bags? [Re: Stromrider]
#19263362 - 12/11/13 10:46 PM (10 years, 1 month ago) |
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OK so I pasteurized 2 spawn bags packed full up to about a foot, and squeezed them side by side in the pressure cooker... It looks like most people pasteurize one bag at a time per PC? is that because the walls of the PC get hotter than the water and heat unevenly? That might be what happened Most of the substrate got up to a peak of 158, but on the side the thermometer probe read 178. Would this still be ok?
They're still cooling off in a tub. If the outsides were partially sterilized, as long as the core (and majority) of the bags were properly pasteurized from 140-158 for an hour, would that thermophilic bacteria spread to the outside parts that were heated up to 178? I assume this could only happen after mixing the substrate up in the tub when spawning... but would that be probable for contams to take hold then?
I did that large amount of substrate because I have 8 quart jars that are clean, and I'm planning on using those to spawn to a large 105-quart tub; putting all my eggs into one basket. It would probably be wiser to use a smaller tub, or two mini-monos (already available, but require more attention i think) with a larger amount of spawn in proportion to substrate, hmm...
what would you all do with this partially sterilized substrate and 8 jars of spawn?
Edited by Generic (12/11/13 10:52 PM)
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PirateSwazey



Registered: 12/12/12
Posts: 2,993
Loc: Here, Now
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Re: Going to start pasteurizing in bags. Oven bags vs filter patch bags? [Re: Generic]
#19263756 - 12/12/13 12:15 AM (10 years, 1 month ago) |
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Hey dude.
Tough to say about your sub... I would still use it but that's me.
I believe you need to go over 180° for 10 minutes or so to start sterilizing it though.
Quote:
Generic said: what would you all do with this partially sterilized substrate and 8 jars of spawn?
I'd spawn 6 of em and G2G the others into 20-25 more jars
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36fuckin5
Alchemycologist


Registered: 08/11/03
Posts: 12,079
Loc: Diving into Mystical Territori...
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Re: Going to start pasteurizing in bags. Oven bags vs filter patch bags? [Re: PirateSwazey]
#19263871 - 12/12/13 12:52 AM (10 years, 1 month ago) |
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Your substrate isn't partially sterilized. It's pasteurized.
-------------------- Redd Foxx said: If you're offended I don't give a shit and don't come see me no more. Pat The Bunny said: A punk rock song won't ever change the world, but I can tell you about a couple that changed me. bodhisatta said: i recommend common sense and figuring it out. These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.
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Generic
Registered: 11/12/13
Posts: 176
Loc: Oregon
Last seen: 1 day, 19 hours
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Re: Going to start pasteurizing in bags. Oven bags vs filter patch bags? [Re: 36fuckin5]
#19263952 - 12/12/13 01:20 AM (10 years, 1 month ago) |
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nice  thanks
is it not ideal to pasteurize two bags in one pressure cooker, though? It seems much better than doing one at a time and waiting an extra 2-3 hours. did i get lucky or is there really no problem? it sure is easy and time-efficient to pasteurize a bunch of substrate
Quote:
I'd spawn 6 of em and G2G the others into 20-25 more jars 
These jars are already on G3, meaning they started from MS, then have been G2G transferred twice. I've really got reconsider my sterile technique, I've had a 75% contamination rate
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PirateSwazey



Registered: 12/12/12
Posts: 2,993
Loc: Here, Now
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Re: Going to start pasteurizing in bags. Oven bags vs filter patch bags? [Re: Generic]
#19263969 - 12/12/13 01:25 AM (10 years, 1 month ago) |
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They're fine to continue G2G.
From what I've noticed it takes me about 5 generations before I really notice the myc slowing down 
I've got one culture that's on its last generation from a syringe that I started growing out back in late august.
As for your sterile technique... you'll get better as time goes by... working with agar helps a lot as well.
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fahtster
Now With 33%More Faht



Registered: 06/17/06
Posts: 9,270
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Re: Going to start pasteurizing in bags. Oven bags vs filter patch bags? [Re: FrankHorrigan]
#19290185 - 12/17/13 07:18 PM (10 years, 1 month ago) |
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Quote:
FrankHorrigan said: That is outdated, bad info. I doubt faht would still stand by that quote you pulled 
Bacterial contams on bulk are almost always caused by poor pasteurization (the heating part) and/or bad grain spawn.
My write up cronic linked for you has everything you need to know.
When the time is up, remove the bag from the water and place it in a box to cool. Don't poke holes or mess with anything. And it doesn't have to be a special box, I just use an old cardboard box.
Yeah, I think the reason that I had to do that was the shear amount of substrate I was pasteurizing. I'm guessing that some the middle of my substrate didn't get pasteurized because it was so thick. Poking the holes in the bag didn't let the totally unpasteurized sit without air; letting the bacteria grow. It worked for what I was doing, but it wasn't the best route to go. nowadays I'd pasteurize in smaller amounts... probably just do more bags (so the thickness is much much less) or just go with the jar method via frank's write-up and lower the times dramatically. I don't have time right now to fix it in my thread, but I will when I get a chance. I did a lot of shit weirdly lol... it worked at the time. Maybe I'm just a lucky dood. Many better methods have been developed since.. Frank's work is a great place to start for that.
faht
Edited by fahtster (12/17/13 07:25 PM)
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PirateSwazey



Registered: 12/12/12
Posts: 2,993
Loc: Here, Now
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Re: Going to start pasteurizing in bags. Oven bags vs filter patch bags? [Re: fahtster]
#19290265 - 12/17/13 07:35 PM (10 years, 1 month ago) |
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Hey faht,
Nice to see you again. Thanks for clearing that pokey thing up
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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Re: Going to start pasteurizing in bags. Oven bags vs filter patch bags? [Re: PirateSwazey]
#19290482 - 12/17/13 08:31 PM (10 years, 1 month ago) |
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--------------------
  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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Generic
Registered: 11/12/13
Posts: 176
Loc: Oregon
Last seen: 1 day, 19 hours
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Re: Going to start pasteurizing in bags. Oven bags vs filter patch bags? [Re: cronicr]
#19348766 - 12/30/13 09:42 PM (10 years, 1 month ago) |
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when pasteurizing filter patch bags, one of them got soaked with water. the only thing i can think of was that because of poking a hole in the bag to probe it with a thermometer, water must have dripped in, but it seems like a ton of water.
Also, the other bags that weren't soaked seemed to have dried out. Is it bad practice to poke a hole through the plastic to put the thermometer in? I've seen in pictures the thermometer just poked into the side/top... would it be better to put the probe through the top of the bag's opening?
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Hereigrow
Stranger

Registered: 01/04/20
Posts: 277
Last seen: 1 year, 5 months
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Re: Going to start pasteurizing in bags. Oven bags vs filter patch bags? [Re: Generic]
#26512818 - 03/02/20 12:53 PM (3 years, 10 months ago) |
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It works in the oven?
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