Home | Community | Message Board


This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: North Spore Bulk Substrate, Cultivation Supplies   Original Sensible Seeds Bulk Cannabis Seeds   Mushroom-Hut Liquid Cultures

Jump to first unread post Pages: < Back | 1 | 2  [ show all ]
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
Invisible36fuckin5
Alchemycologist


Registered: 08/11/03
Posts: 12,079
Loc: Diving into Mystical Territori...
Trusted Cultivator
Re: Going to start pasteurizing in bags. Oven bags vs filter patch bags? [Re: Stromrider]
    #19249074 - 12/09/13 07:58 AM (10 years, 1 month ago)

I throw my hot substrate into my tub and mix it till it's cool enough to spawn. Mostly just to make things go quicker.


--------------------
Redd Foxx said:
If you're offended I don't give a shit and don't come see me no more.

Pat The Bunny said:
A punk rock song won't ever change the world, but I can tell you about a couple that changed me.

bodhisatta said:
i recommend common sense and figuring it out.

These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.


Extras: Filter Print Post Top
OfflineStromriderM
This must be the place
Male User Gallery


Registered: 06/02/13
Posts: 7,326
Loc: Dept of know what I'm say... Flag
Last seen: 39 minutes, 8 seconds
Trusted Cultivator
Re: Going to start pasteurizing in bags. Oven bags vs filter patch bags? [Re: 36fuckin5]
    #19249091 - 12/09/13 08:06 AM (10 years, 1 month ago)

Yeah I've done that as well


Extras: Filter Print Post Top
OfflineGeneric
Registered: 11/12/13
Posts: 176
Loc: Oregon
Last seen: 1 day, 19 hours
Re: Going to start pasteurizing in bags. Oven bags vs filter patch bags? [Re: Stromrider]
    #19263362 - 12/11/13 10:46 PM (10 years, 1 month ago)

OK so I pasteurized 2 spawn bags packed full up to about a foot, and squeezed them side by side in the pressure cooker...  It looks like most people pasteurize one bag at a time per PC?  is that because the walls of the PC get hotter than the water and heat unevenly?  That might be what happened :frown:  Most of the substrate got up to a peak of 158, but on the side the thermometer probe read 178.  Would this still be ok? 

They're still cooling off in a tub.  If the outsides were partially sterilized, as long as the core (and majority) of the bags were properly pasteurized from 140-158 for an hour, would that thermophilic bacteria spread to the outside parts that were heated up to 178?  I assume this could only happen after mixing the substrate up in the tub when spawning... but would that be probable for contams to take hold then?

I did that large amount of substrate because I have 8 quart jars that are clean, and I'm planning on using those to spawn to a large 105-quart tub; putting all my eggs into one basket.  It would probably be wiser to use a smaller tub, or two mini-monos (already available, but require more attention i think) with a larger amount of spawn in proportion to substrate, hmm...

what would you all do with this partially sterilized substrate and 8 jars of spawn?


Edited by Generic (12/11/13 10:52 PM)


Extras: Filter Print Post Top
InvisiblePirateSwazey
 User Gallery


Registered: 12/12/12
Posts: 2,993
Loc: Here, Now
Trusted Cultivator
Re: Going to start pasteurizing in bags. Oven bags vs filter patch bags? [Re: Generic]
    #19263756 - 12/12/13 12:15 AM (10 years, 1 month ago)

Hey dude.

Tough to say about your sub... I would still use it but that's me.

I believe you need to go over 180° for 10 minutes or so to start sterilizing it though.

Quote:

Generic said:
what would you all do with this partially sterilized substrate and 8 jars of spawn?




I'd spawn 6 of em and G2G the others into 20-25 more jars :dancer:


--------------------
 

BULK RYE PREP  -  MY FAVORITE THREADS  -  BUILD A FLOWHOOD



Extras: Filter Print Post Top
Invisible36fuckin5
Alchemycologist


Registered: 08/11/03
Posts: 12,079
Loc: Diving into Mystical Territori...
Trusted Cultivator
Re: Going to start pasteurizing in bags. Oven bags vs filter patch bags? [Re: PirateSwazey]
    #19263871 - 12/12/13 12:52 AM (10 years, 1 month ago)

Your substrate isn't partially sterilized. It's pasteurized.


--------------------
Redd Foxx said:
If you're offended I don't give a shit and don't come see me no more.

Pat The Bunny said:
A punk rock song won't ever change the world, but I can tell you about a couple that changed me.

bodhisatta said:
i recommend common sense and figuring it out.

These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.


Extras: Filter Print Post Top
OfflineGeneric
Registered: 11/12/13
Posts: 176
Loc: Oregon
Last seen: 1 day, 19 hours
Re: Going to start pasteurizing in bags. Oven bags vs filter patch bags? [Re: 36fuckin5]
    #19263952 - 12/12/13 01:20 AM (10 years, 1 month ago)

nice :smile:
thanks

is it not ideal to pasteurize two bags in one pressure cooker, though?
It seems much better than doing one at a time and waiting an extra 2-3 hours.
did i get lucky or is there really no problem?  it sure is easy and time-efficient to pasteurize a bunch of substrate

Quote:

I'd spawn 6 of em and G2G the others into 20-25 more jars :dancer:


 
These jars are already on G3, meaning they started from MS, then have been G2G transferred twice.  I've really got reconsider my sterile technique, I've had a 75% contamination rate :facepalm:


Extras: Filter Print Post Top
InvisiblePirateSwazey
 User Gallery


Registered: 12/12/12
Posts: 2,993
Loc: Here, Now
Trusted Cultivator
Re: Going to start pasteurizing in bags. Oven bags vs filter patch bags? [Re: Generic]
    #19263969 - 12/12/13 01:25 AM (10 years, 1 month ago)

They're fine to continue G2G.

From what I've noticed it takes me about 5 generations before I really notice the myc slowing down :thumbup:

I've got one culture that's on its last generation from a syringe that I started growing out back in late august.

As for your sterile technique... you'll get better as time goes by... working with agar helps a lot as well.


--------------------
 

BULK RYE PREP  -  MY FAVORITE THREADS  -  BUILD A FLOWHOOD



Extras: Filter Print Post Top
InvisiblefahtsterM
Now With 33%More Faht
Male User Gallery


Registered: 06/17/06
Posts: 9,270
Trusted Cultivator
OG Cultivator
Re: Going to start pasteurizing in bags. Oven bags vs filter patch bags? [Re: FrankHorrigan]
    #19290185 - 12/17/13 07:18 PM (10 years, 1 month ago)

Quote:

FrankHorrigan said:
That is outdated, bad info. I doubt faht would still stand by that quote you pulled :shrug:

Bacterial contams on bulk are almost always caused by poor pasteurization (the heating part) and/or bad grain spawn.

My write up cronic linked for you has everything you need to know.

When the time is up, remove the bag from the water and place it in a box to cool. Don't poke holes or mess with anything. And it doesn't have to be a special box, I just use an old cardboard box.




Yeah, I think the reason that I had to do that was the shear amount of substrate I was pasteurizing.  I'm guessing that some the middle of my substrate didn't get pasteurized because it was so thick.  Poking the holes in the bag didn't let the totally unpasteurized sit without air; letting the bacteria grow.  It worked for what I was doing, but it wasn't the best route to go.  nowadays I'd pasteurize in smaller amounts... probably just do more bags (so the thickness is much much less) or just go with the jar method via frank's write-up and lower the times dramatically. I don't have time right now to fix it in my thread, but I will when I get a chance.  I did a lot of shit weirdly lol... it worked at the time.  Maybe I'm just a lucky dood.  Many better methods have been developed since.. Frank's work is a great place to start for that.

:thumbup: faht


Edited by fahtster (12/17/13 07:25 PM)


Extras: Filter Print Post Top
InvisiblePirateSwazey
 User Gallery


Registered: 12/12/12
Posts: 2,993
Loc: Here, Now
Trusted Cultivator
Re: Going to start pasteurizing in bags. Oven bags vs filter patch bags? [Re: fahtster]
    #19290265 - 12/17/13 07:35 PM (10 years, 1 month ago)

Hey faht,

Nice to see you again.
Thanks for clearing that pokey thing up :jah:


--------------------
 

BULK RYE PREP  -  MY FAVORITE THREADS  -  BUILD A FLOWHOOD



Extras: Filter Print Post Top
OfflinecronicrFacebook
 User Gallery


Registered: 08/07/11
Posts: 61,436
Loc: Van Isle Flag
Last seen: 2 years, 8 days
Trusted Cultivator
Re: Going to start pasteurizing in bags. Oven bags vs filter patch bags? [Re: PirateSwazey]
    #19290482 - 12/17/13 08:31 PM (10 years, 1 month ago)

:whathesaid:


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


Extras: Filter Print Post Top
OfflineGeneric
Registered: 11/12/13
Posts: 176
Loc: Oregon
Last seen: 1 day, 19 hours
Re: Going to start pasteurizing in bags. Oven bags vs filter patch bags? [Re: cronicr]
    #19348766 - 12/30/13 09:42 PM (10 years, 1 month ago)

when pasteurizing filter patch bags, one of them got soaked with water.  the only thing i can think of was that because of poking a hole in the bag to probe it with a thermometer, water must have dripped in, but it seems like a ton of water. 

Also, the other bags that weren't soaked seemed to have dried out.  Is it bad practice to poke a hole through the plastic to put the thermometer in?  I've seen in pictures the thermometer just poked into the side/top...  would it be better to put the probe through the top of the bag's opening?


Extras: Filter Print Post Top
OfflineHereigrow
Stranger
 User Gallery
Registered: 01/04/20
Posts: 277
Last seen: 1 year, 5 months
Re: Going to start pasteurizing in bags. Oven bags vs filter patch bags? [Re: Generic]
    #26512818 - 03/02/20 12:53 PM (3 years, 10 months ago)

It works in the oven?


Extras: Filter Print Post Top
Jump to top Pages: < Back | 1 | 2  [ show all ]

Shop: North Spore Bulk Substrate, Cultivation Supplies   Original Sensible Seeds Bulk Cannabis Seeds   Mushroom-Hut Liquid Cultures


Similar ThreadsPosterViewsRepliesLast post
* Oven-Bag Pasteurization "Tek" Pictorial angryshroom 21,099 8 04/02/03 09:16 AM
by pillsbury420
* oven bags user_exists 1,533 8 02/01/03 06:23 PM
by SixTango
* Oven bag in Oven?!?!?!?!?! wikedanjel 1,977 1 07/08/02 04:33 AM
by mycofile
* Growing With Oven Bags And BRF TripsAreForKids 1,496 3 08/31/02 02:49 PM
by trainboy55
* oven bags, ph balancing, etc. acidhead1279 1,172 9 08/17/03 05:31 PM
by acidhead1279
* reynolds oven bags in pressure cooker? BIGSWANG 9,767 3 03/06/02 08:34 PM
by BIGSWANG
* OVEN BAGS! Sterile 2,554 8 05/15/01 12:11 PM
by Myconoclastic
* Exploding Oven Bags effwun 2,503 8 10/28/01 11:39 AM
by Anonymous

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
7,241 topic views. 43 members, 209 guests and 23 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.022 seconds spending 0.009 seconds on 14 queries.