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Dirtygoat
Bread



Registered: 10/22/13
Posts: 561
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Making LC
#19230828 - 12/05/13 11:37 AM (10 years, 2 months ago) |
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I'm about to try to make LC as my syringe is running low, and I don't want to rely on making my first spore print being sterile.
I was just wondering, can the LC colonize too much? If I use a tiny bit, and let the rest sit for up to a year, will it eventually get too colonized and too thick for my 16g needle?
Also, I'm pretty sure the answer is yes, but can I just use some LC to make more LC? I've only seen people say to inject with spores, but never inject with something else, I just was wondering if the first LC I make will be able to last me forever without any more spores.
Lastly, agar is confusing as nuts.. And I don't want to receive mail if I were to buy premade agar dishes. I was wondering if I can make LC using mushroom tissue? Of course sterility plays a big part if I try this, but would it work?
Thanks for any help guys
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PussyFart
Retired Cultivation Extrodinaire



Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
Last seen: 1 month, 11 days
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Agar is anything but confusing.....you mix agar and a nutrient with water and PC......then pour into sterile dishes in front of a flow hood or in a SAB.
It's just like making jello honestly....
Yes you can make an LC from spores and/or tissue.
It is recommended however to steer away from making LCs from anything but a clean agar wedge.
You can just suck up some sterile water into the syringe and have more spore solution.
The dilution should not really matter that much.
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THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT! A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD, SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List, EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE *** *** AFGHAN KUSH GROW LOG *** ***
Edited by PussyFart (12/05/13 11:45 AM)
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JohnnieYen
Okay



Registered: 03/15/11
Posts: 3,529
Loc: City Z
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I would try agar, it's not very difficult at all. Do you still have grain spawn to do a grain to grain?
or maybe knock up another grain jar and when that is fully colonized, G2G that.
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twistedty
Forcefully Retired



Registered: 07/01/12
Posts: 5,487
Loc: Middle
Last seen: 3 years, 7 months
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agar is much less complicated than any LC recipe and requires a lot less equipment.
just cause youre using agar doesnt mean you have to start isolating or cloning or any of that jazz.
it just ensures a much easier cleaner culture to use.
https://www.shroomery.org/forums/showflat.php/Number/19208415
this is a good tek to follow, and 90-100% of the items needed to do agar can be found at your local food marts.
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twistedty
Forcefully Retired



Registered: 07/01/12
Posts: 5,487
Loc: Middle
Last seen: 3 years, 7 months
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basically find an agar recipe that you think would be easiest to make and then mix it up pour into mini gladware pp5 round containers and pc.
if you contam a few of your dishes you are still able to transfer away from the contam to another dish and create a clean culture, unlike LC.
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kushman88
Stranger


Registered: 10/20/13
Posts: 78
Last seen: 2 years, 4 months
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I know I'm a noob without much post but i just had a successful agar attempt...real easy to i did the brf agar and pc jar for 20 mins and then i dropped two drops 1/5 cc of penis envy ms and 12 days later i had a healthy myceluim covering almost 80% then i got a empty syringe sucked boiling water in the syringe and released into the sink i did this 5 times and the 6th time on me sucking the water i just left it to cool overnight...next morning i scrapped the top of the agar of all mycelium and suck it all up i lost 1 cc couldnt suck it all up....so from there i prepped 12 wbs jars and pc'd 90 mins cool those overnight and NOW after EXACTLY 3 days i have at least 15% growth in 11 of 12 jars....OVERALL agar was SIMPLE and all work was done in SAB
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twistedty
Forcefully Retired



Registered: 07/01/12
Posts: 5,487
Loc: Middle
Last seen: 3 years, 7 months
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Quote:
kushman88 said: I know I'm a noob without much post but i just had a successful agar attempt...real easy to i did the brf agar and pc jar for 20 mins and then i dropped two drops 1/5 cc of penis envy ms and 12 days later i had a healthy myceluim covering almost 80% then i got a empty syringe sucked boiling water in the syringe and released into the sink i did this 5 times and the 6th time on me sucking the water i just left it to cool overnight...next morning i scrapped the top of the agar of all mycelium and suck it all up i lost 1 cc couldnt suck it all up....so from there i prepped 12 wbs jars and pc'd 90 mins cool those overnight and NOW after EXACTLY 3 days i have at least 15% growth in 11 of 12 jars....OVERALL agar was SIMPLE and all work was done in SAB
next time just heat up a blade red hot and cut a wedge and drop it directly into the grain jar via SAB aka master jar.
then g2g your heart out with however many master jars you made.
for example i do 10 master jars then turn those 10 into a 100 via g2g.
best way to do bulk
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Dirtygoat
Bread



Registered: 10/22/13
Posts: 561
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One thing that gets me a little frustrated is that people on here expect everyone to have PCs.. I don't, and I think I can make LC with just steam.
Sterility is my main concern. I don't have a large workspace. And I have cats, so sticking with pf tek is my best bet and it is working out so far..
Agar seems complicated because of all the ingredients I need and money I need to spend.. LC can be made from ingredients I already have.
So, I think I got the answers I needed, thanks guys. My main question was can LC get too think, but I now realize probably not, unless I put too much substrate? into the water.. Also I wanted to know if cloning works with LC in case I get one really nice mushroom I would like to make more of.
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Josh.0
ConnoissurOfSorts


Registered: 11/25/13
Posts: 553
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with your limited resources..GLC FTW
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Josh.0
ConnoissurOfSorts


Registered: 11/25/13
Posts: 553
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Quote:
twistedty said:

you pizzaed when you shoulda french fried
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,811
Loc: Canada
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Agar can be made from the same ingredients as LC, just need to add agar. If your determined that LC can work and agar is too hard then fine. LC can work, and people can win the lottery. No one is trying to steer you wrong, we are try to help you gain success. Do a search on contamed LC and have a look at how many people felt the same as you did and learned the hard way.
Personally I would never inoculate a LC with anything but a clean agar wedge
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Dirtygoat
Bread



Registered: 10/22/13
Posts: 561
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No I understand, and I thank you all very much for all the great info I can get with such ease.. But I don't have a PC lol.... I'll get one eventually. But for now, thanks, I will just experiment with what I have, and I'll prob show you all my results.
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DontPlay
Yeah Science!



Registered: 12/13/07
Posts: 750
Loc: On Land most of the time....
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Iv'e been wanting to try agar cultures for a while, but hate having to buy more supplies.
I tried my first organic honey liquid culture following a tek. And inoculated it with a syringe. The jar has about 600ml in it and I pumped about 2cc's into it. After a week I am already seeing good mycelium growth.
Only thing is the syringe was pretty thick with spores and their are still alot of spores sitting on the bottom of the LC. Not sure if they are going to germinate.
-------------------- My Trade List I hate to advocate drugs, alcohol, violence, or insanity to anyone, but they've always worked for me. Hunter S. Thompson
I prefer dangerous freedom over peaceful slavery. ~Thomas Jefferson
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Dirtygoat
Bread



Registered: 10/22/13
Posts: 561
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Do you still have that tek link? I pretty much know what I will be doing, but more options are always helpful.
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Fred Teddy
Flying with Turkeys


Registered: 07/18/11
Posts: 208
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If you have a pc then LC can work. If you don't, it won't work. Not trying to steer you away from LC, to each will learn, I do it myself at times but never more than 75% successful, and its hard enough to accomplish with a pc, I cant see doing it without one. I'll fool around with it, but for serious work its agar to grains. Your spore print will not be sterile, none is. You say sterility is a concern ... so ... I would use the search function and research agar. Its not as bad as you make it sound, and Telephone Brand agar is $2.49/pkg (can do lots and lots of plates or jars) at an Asian market. Keep the spore prints for when you have agar supplies and you will find that cleaning them up is easy with agar.
Peace, FT
-------------------- ... its all about fun and games ... and no one has poked their eye out ... yet. Peace. Disclaimer: Fact is ... Its all fiction.
Edited by Fred Teddy (12/05/13 01:45 PM)
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Dirtygoat
Bread



Registered: 10/22/13
Posts: 561
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Does agar need a PC?
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DontPlay
Yeah Science!



Registered: 12/13/07
Posts: 750
Loc: On Land most of the time....
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The tek I tried recently for the organic honey LC http://www.shroomery.org/9145/Liquid-Culture-Basics
It actually has instructions for all LC methods...Honey, Malt, Dextrose etc. I do have a PC but opted to try and use the boiling method which so far has worked. My very first grow ever didn't have a PC and used rather unsterile methods and had great success.
I'm big on doing things the simple way, not everyone has the space or money to have an amazing setup.
-------------------- My Trade List I hate to advocate drugs, alcohol, violence, or insanity to anyone, but they've always worked for me. Hunter S. Thompson
I prefer dangerous freedom over peaceful slavery. ~Thomas Jefferson
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Josh.0
ConnoissurOfSorts


Registered: 11/25/13
Posts: 553
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 you need not PC whole brown rice.. picture was taken a week after making a couple of  just a suggestion i thought to add.. but by all means carry on with your honey/karo concoctions..
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fractaldill
Cultivator in Training

Registered: 08/31/13
Posts: 909
Last seen: 9 years, 10 months
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Quote:
Notahacker420 said: You can just suck up some sterile water into the syringe and have more spore solution
never thought of that
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Dirtygoat
Bread



Registered: 10/22/13
Posts: 561
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Quote:
DontPlay said: The tek I tried recently for the organic honey LC http://www.shroomery.org/9145/Liquid-Culture-Basics
It actually has instructions for all LC methods...Honey, Malt, Dextrose etc. I do have a PC but opted to try and use the boiling method which so far has worked. My very first grow ever didn't have a PC and used rather unsterile methods and had great success.
I'm big on doing things the simple way, not everyone has the space or money to have an amazing setup.
Awesome, thanks. I've been looking for a page like is, idk how I haven't read it yet lol.
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