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twistedty
Forcefully Retired



Registered: 07/01/12
Posts: 5,487
Loc: Middle
Last seen: 3 years, 7 months
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Re: Black mold? or some other kind of contam? (pictures included) [Re: dessidoll93]
#19230910 - 12/05/13 12:02 PM (10 years, 2 months ago) |
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Quote:
dessidoll93 said: I steamed them in a large pot. I do have a 1/2 inch verm layer. I did flame my needle, as well as wiped it with alcohol. BUT I did inoculate in open air but there wasn't any air on and I disinfected as best as I could. I'm just so sad to seem them go!
you should only flame sterilize, if you flame sterilize then wipe with anything you need to flame sterilize again.
not saying this lead to your failure that is if its contammed
Edited by twistedty (12/05/13 12:02 PM)
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elasticaltiger
Like Tigers in Coitus




Registered: 06/24/13
Posts: 8,409
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Re: Black mold? or some other kind of contam? (pictures included) [Re: twistedty]
#19230938 - 12/05/13 12:08 PM (10 years, 2 months ago) |
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Quote:
twistedty said:
Quote:
dessidoll93 said: I steamed them in a large pot. I do have a 1/2 inch verm layer. I did flame my needle, as well as wiped it with alcohol. BUT I did inoculate in open air but there wasn't any air on and I disinfected as best as I could. I'm just so sad to seem them go!
you should only flame sterilize, if you flame sterilize then wipe with anything you need to flame sterilize again.
not saying this lead to your failure that is if its contammed
Beat me to it. Flame sterilization is all you need. Heat will kill everything. Alcohol will only kill 99.99% of contamination.
-------------------- First time growing cakes? DON'T make a Shotgun Fruiting Chamber The Shmuvbox. - The Old TC's Like it Afraid to Start Growing From Your Own Prints? Drop it Like a Tiger! No Pouring. No Syringes. No Cutting. No flaming. No Contamination. No Bullshit. "The best thing to do while your waiting is to start more stuff. I usually got so much happening that I have tossed projects simply because I didn't have time for them. -Pastywhite QFT Pastywhite's Easy Agar Tek (PastyPlates) Tiger Drop Video Demos By munchauzen Van Gogh would’ve sold more than one painting if he’d put tigers in them.―Bill Watterson EZEKIEL 23:20
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Irfan
Stranger

Registered: 09/06/13
Posts: 180
Last seen: 10 years, 2 months
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Re: Black mold? or some other kind of contam? (pictures included) [Re: dessidoll93]
#19230964 - 12/05/13 12:14 PM (10 years, 2 months ago) |
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Quote:
dessidoll93 said: I steamed them in a large pot. I do have a 1/2 inch verm layer. I did flame my needle, as well as wiped it with alcohol. BUT I did inoculate in open air but there wasn't any air on and I disinfected as best as I could. I'm just so sad to seem them go!
Black molds can cause serious permanent health problems. I am empathetic but its best to let them go...
Buy a pressure cooker.
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Josh.0
ConnoissurOfSorts


Registered: 11/25/13
Posts: 553
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Re: Black mold? or some other kind of contam? (pictures included) [Re: twistedty]
#19230984 - 12/05/13 12:20 PM (10 years, 2 months ago) |
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Quote:
dessidoll93 said: I'm just so sad to seem them go!
think of how happy your going to be when the next set of jars pans out just as you hoped
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Kizzle
Misanthrope


Registered: 08/30/11
Posts: 9,865
Last seen: 2 days, 38 minutes
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Re: Black mold? or some other kind of contam? (pictures included) [Re: Irfan]
#19231073 - 12/05/13 12:47 PM (10 years, 2 months ago) |
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I've never used any the syringes with charcoal but this is supposedly what they're supposed to look like. I'd check the site of the place got it from and see if it mentions it.

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2bittoker
Resident PMP Advocate


Registered: 03/09/13
Posts: 555
Last seen: 2 years, 4 months
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Re: Black mold? or some other kind of contam? (pictures included) [Re: elasticaltiger]
#19231667 - 12/05/13 03:05 PM (10 years, 2 months ago) |
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Quote:
elasticaltiger said:
Quote:
twistedty said:
Quote:
dessidoll93 said: I steamed them in a large pot. I do have a 1/2 inch verm layer. I did flame my needle, as well as wiped it with alcohol. BUT I did inoculate in open air but there wasn't any air on and I disinfected as best as I could. I'm just so sad to seem them go!
you should only flame sterilize, if you flame sterilize then wipe with anything you need to flame sterilize again.
not saying this lead to your failure that is if its contammed
Beat me to it. Flame sterilization is all you need. Heat will kill everything. Alcohol will only kill 99.99% of contamination.
Negative. Alcohol only kills bacteria (at 70-30 mix), a SMALL amount viruses. It doesnt do jack to Mold or mold spores, like Trich Spores. Wiping down the needle with alcohol should only be done if there's something on it that you dont want to burn, like extra verm between jars.
-------------------- “I slept and dreamt that life was joy. I awoke and saw that life was service. I acted and behold, service was joy.” "Love does not claim possession, but gives freedom" ― Rabindranath Tagore Stuff for New Growers Where new growers should start: RogerRabbit's PF Tek video How it Should and Shouldn't Look My Simplified Bulk Growing My OJ Shroom Tek
Edited by 2bittoker (12/05/13 03:06 PM)
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PussyFart
Retired Cultivation Extrodinaire



Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
Last seen: 1 month, 11 days
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Re: Black mold? or some other kind of contam? (pictures included) [Re: 2bittoker]
#19231921 - 12/05/13 04:00 PM (10 years, 2 months ago) |
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Wiping a flame sterilized tool with an unsterile substance like alcohol is like wiping shit on your face after a shower....
-RR
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THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT! A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD, SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List, EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE *** *** AFGHAN KUSH GROW LOG *** ***
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sharpshroomer98
Student of the Universe.



Registered: 08/30/11
Posts: 252
Last seen: 1 day, 3 hours
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Re: Black mold? or some other kind of contam? (pictures included) [Re: PussyFart]
#19231932 - 12/05/13 04:02 PM (10 years, 2 months ago) |
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Quote:
Notahacker420 said: Wiping a flame sterilized tool with an unsterile substance like alcohol is like wiping shit on your face after a shower....
-RR
Mycology logic lol,, it's the best.
-------------------- No, I wasn’t born in 98’
 
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2bittoker
Resident PMP Advocate


Registered: 03/09/13
Posts: 555
Last seen: 2 years, 4 months
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Re: Black mold? or some other kind of contam? (pictures included) [Re: PussyFart]
#19233027 - 12/05/13 05:51 PM (10 years, 2 months ago) |
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Quote:
Notahacker420 said: Wiping a flame sterilized tool with an unsterile substance like alcohol is like wiping shit on your face after a shower....
-RR
I hope that wasnt in reply to me. Since I was talking about cleaning off needles of debris gathered between jars, before flame sterilizing. And needles after inoculating jars and being exposed to open air are assumed to be no longer sterile, hence flame sterlizing between jars.
-------------------- “I slept and dreamt that life was joy. I awoke and saw that life was service. I acted and behold, service was joy.” "Love does not claim possession, but gives freedom" ― Rabindranath Tagore Stuff for New Growers Where new growers should start: RogerRabbit's PF Tek video How it Should and Shouldn't Look My Simplified Bulk Growing My OJ Shroom Tek
Edited by 2bittoker (12/05/13 09:30 PM)
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