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InvisibleDirtygoat
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Making LC
    #19230828 - 12/05/13 11:37 AM (10 years, 1 month ago)

I'm about to try to make LC as my syringe is running low, and I don't want to rely on making my first spore print being sterile.

I was just wondering, can the LC colonize too much? If I use a tiny bit, and let the rest sit for up to a year, will it eventually get too colonized and too thick for my 16g needle?

Also, I'm pretty sure the answer is yes, but can I just use some LC to make more LC? I've only seen people say to inject with spores, but never inject with something else, I just was wondering if the first LC I make will be able to last me forever without any more spores.

Lastly, agar is confusing as nuts.. And I don't want to receive mail if I were to buy premade agar dishes. I was wondering if I can make LC using mushroom tissue? Of course sterility plays a big part if I try this, but would it work?

Thanks for any help guys :grin:


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OfflinePussyFart
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Re: Making LC [Re: Dirtygoat] * 1
    #19230853 - 12/05/13 11:45 AM (10 years, 1 month ago)

Agar is anything but confusing.....you mix agar and a nutrient with water and PC......then pour into sterile dishes in front of a flow hood or in a SAB.

It's just like making jello honestly....

Yes you can make an LC from spores and/or tissue.

It is recommended however to steer away from making LCs from anything but a clean agar wedge.

You can just suck up some sterile water into the syringe and have more spore solution.

The dilution should not really matter that much.


Edited by PussyFart (12/05/13 11:45 AM)


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InvisibleJohnnieYen
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Re: Making LC [Re: Dirtygoat]
    #19230870 - 12/05/13 11:48 AM (10 years, 1 month ago)

I would try agar, it's not very difficult at all. Do you still have grain spawn to do a grain to grain?

or maybe knock up another grain jar and when that is fully colonized, G2G that. :toast:


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Offlinetwistedty
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Re: Making LC [Re: PussyFart]
    #19230874 - 12/05/13 11:50 AM (10 years, 1 month ago)

agar is much less complicated than any LC recipe and requires a lot less equipment.

just cause youre using agar doesnt mean you have to start isolating or cloning or any of that jazz.

it just ensures a much easier cleaner culture to use.


https://www.shroomery.org/forums/showflat.php/Number/19208415

this is a good tek to follow, and 90-100% of the items needed to do agar can be found at your local food marts.


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Re: Making LC [Re: twistedty]
    #19230883 - 12/05/13 11:54 AM (10 years, 1 month ago)

basically find an agar recipe that you think would be easiest to make and then mix it up pour into mini gladware pp5 round containers and pc.

if you contam a few of your dishes you are still able to transfer away from the contam to another dish and create a clean culture, unlike LC.




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Offlinekushman88
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Re: Making LC [Re: JohnnieYen]
    #19230932 - 12/05/13 12:07 PM (10 years, 1 month ago)

I know I'm a noob without much post but i just had a successful agar attempt...real easy to i did the brf agar and pc jar for 20 mins and then i dropped two drops 1/5 cc of penis envy ms and 12 days later i had a healthy myceluim covering almost 80% then i got a empty syringe sucked boiling water in the syringe and released into the sink i did this 5 times and the 6th time on me sucking the water i just left it to cool overnight...next morning i scrapped the top of the agar of all mycelium and suck it all up i lost 1 cc couldnt suck it all up....so from there i prepped 12 wbs jars and pc'd 90 mins cool those overnight and NOW after EXACTLY 3 days i have at least 15% growth in 11 of 12 jars....OVERALL agar was SIMPLE and all work was done in SAB


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Offlinetwistedty
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Re: Making LC [Re: kushman88]
    #19230945 - 12/05/13 12:09 PM (10 years, 1 month ago)

Quote:

kushman88 said:
I know I'm a noob without much post but i just had a successful agar attempt...real easy to i did the brf agar and pc jar for 20 mins and then i dropped two drops 1/5 cc of penis envy ms and 12 days later i had a healthy myceluim covering almost 80% then i got a empty syringe sucked boiling water in the syringe and released into the sink i did this 5 times and the 6th time on me sucking the water i just left it to cool overnight...next morning i scrapped the top of the agar of all mycelium and suck it all up i lost 1 cc couldnt suck it all up....so from there i prepped 12 wbs jars and pc'd 90 mins cool those overnight and NOW after EXACTLY 3 days i have at least 15% growth in 11 of 12 jars....OVERALL agar was SIMPLE and all work was done in SAB





next time just heat up a blade red hot and cut a wedge and drop it directly into the grain jar via SAB aka master jar.

then g2g your heart out with however many master jars you made.

for example i do 10 master jars then turn those 10 into a 100 via g2g.

best way to do bulk :likeaboss:


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InvisibleDirtygoat
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Re: Making LC [Re: twistedty]
    #19230990 - 12/05/13 12:21 PM (10 years, 1 month ago)

One thing that gets me a little frustrated is that people on here expect everyone to have PCs.. I don't, and I think I can make LC with just steam.

Sterility is my main concern. I don't have a large workspace. And I have cats, so sticking with pf tek is my best bet and it is working out so far..

Agar seems complicated because of all the ingredients I need and money I need to spend.. LC can be made from ingredients I already have.


So, I think I got the answers I needed, thanks guys. My main question was can LC get too think, but I now realize probably not, unless I put too much substrate? into the water.. Also I wanted to know if cloning works with LC in case I get one really nice mushroom I would like to make more of.


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InvisibleJosh.0
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Re: Making LC [Re: Dirtygoat]
    #19231040 - 12/05/13 12:34 PM (10 years, 1 month ago)

with your limited resources..GLC FTW:thumbup:


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InvisibleJosh.0
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Re: Making LC [Re: twistedty]
    #19231045 - 12/05/13 12:37 PM (10 years, 1 month ago)

Quote:

twistedty said:





you pizzaed when you shoulda french fried :lol:


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InvisiblePastywhyteMDiscord
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Re: Making LC [Re: Dirtygoat] * 1
    #19231046 - 12/05/13 12:37 PM (10 years, 1 month ago)

Agar can be made from the same ingredients as LC, just need to add agar. If your determined that LC can work and agar is too hard then fine. LC can work, and people can win the lottery. No one is trying to steer you wrong, we are try to help you gain success. Do a search on contamed LC and have a look at how many people felt the same as you did and learned the hard way.

Personally I would never inoculate a LC with anything but a clean agar wedge :shrug:


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InvisibleDirtygoat
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Re: Making LC [Re: Pastywhyte]
    #19231177 - 12/05/13 01:09 PM (10 years, 1 month ago)

No I understand, and I thank you all very much for all the great info I can get with such ease.. But I don't have a PC lol.... I'll get one eventually. But for now, thanks, I will just experiment with what I have, and I'll prob show you all my results.


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InvisibleDontPlay
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Re: Making LC [Re: Dirtygoat]
    #19231200 - 12/05/13 01:16 PM (10 years, 1 month ago)

Iv'e been wanting to try agar cultures for a while, but hate having to buy more supplies.

I tried my first organic honey liquid culture following a tek. And inoculated it with a syringe. The jar has about 600ml in it and I pumped about 2cc's into it. After a week I am already seeing good mycelium growth.

Only thing is the syringe was pretty thick with spores and their are still alot of spores sitting on the bottom of the LC. Not sure if they are going to germinate.


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InvisibleDirtygoat
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Re: Making LC [Re: DontPlay]
    #19231231 - 12/05/13 01:24 PM (10 years, 1 month ago)

Do you still have that tek link? I pretty much know what I will be doing, but more options are always helpful.


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InvisibleFred Teddy
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Re: Making LC [Re: Dirtygoat] * 1
    #19231279 - 12/05/13 01:34 PM (10 years, 1 month ago)

If you have a pc then LC can work. If you don't, it won't work. Not trying to steer you away from LC, to each will learn, I do it myself at times but never more than 75% successful, and its hard enough to accomplish with a pc, I cant see doing it without one. I'll fool around with it, but for serious work its agar to grains. Your spore print will not be sterile, none is. You say sterility is a concern ... so ... I would use the search function and research agar. Its not as bad as you make it sound, and Telephone Brand agar is $2.49/pkg (can do lots and lots of plates or jars) at an Asian market. Keep the spore prints for when you have agar supplies and you will find that cleaning them up is easy with agar.

Peace,
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Edited by Fred Teddy (12/05/13 01:45 PM)


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InvisibleDirtygoat
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Re: Making LC [Re: Fred Teddy]
    #19231312 - 12/05/13 01:40 PM (10 years, 1 month ago)

Does agar need a PC?


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InvisibleDontPlay
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Re: Making LC [Re: Fred Teddy]
    #19231321 - 12/05/13 01:43 PM (10 years, 1 month ago)

The tek I tried recently for the organic honey LC
http://www.shroomery.org/9145/Liquid-Culture-Basics

It actually has instructions for all LC methods...Honey, Malt, Dextrose etc. I do have a PC but opted to try and use the boiling method which so far has worked. My very first grow ever didn't have a PC and used rather unsterile methods and had great success.

I'm big on doing things the simple way, not everyone has the space or money to have an amazing setup.


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InvisibleJosh.0
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Re: Making LC [Re: Fred Teddy]
    #19231326 - 12/05/13 01:43 PM (10 years, 1 month ago)


you need not PC whole brown rice..:shrug:
picture was taken a week after making a couple of :syringe:
just a suggestion i thought to add..
but by all means carry on with your honey/karo concoctions..
:scarygrin:


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Offlinefractaldill
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Re: Making LC [Re: PussyFart]
    #19231360 - 12/05/13 01:52 PM (10 years, 1 month ago)

Quote:

Notahacker420 said:
You can just suck up some sterile water into the syringe and have more spore solution




never thought of that  :wow:


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InvisibleDirtygoat
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Re: Making LC [Re: DontPlay]
    #19231425 - 12/05/13 02:09 PM (10 years, 1 month ago)

Quote:

DontPlay said:
The tek I tried recently for the organic honey LC
http://www.shroomery.org/9145/Liquid-Culture-Basics

It actually has instructions for all LC methods...Honey, Malt, Dextrose etc. I do have a PC but opted to try and use the boiling method which so far has worked. My very first grow ever didn't have a PC and used rather unsterile methods and had great success.

I'm big on doing things the simple way, not everyone has the space or money to have an amazing setup.




Awesome, thanks. I've been looking for a page like is, idk how I haven't read it yet lol.


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Offlinetwistedty
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Re: Making LC [Re: Pastywhyte]
    #19231788 - 12/05/13 03:32 PM (10 years, 1 month ago)

Quote:

DontPlay said:
Iv'e been wanting to try agar cultures for a while, but hate having to buy more supplies.

I tried my first organic honey liquid culture following a tek. And inoculated it with a syringe. The jar has about 600ml in it and I pumped about 2cc's into it. After a week I am already seeing good mycelium growth.

Only thing is the syringe was pretty thick with spores and their are still alot of spores sitting on the bottom of the LC. Not sure if they are going to germinate.





you still dont know if your LC is clean or not until you do some test jars which will take another 2-4weeks.




Quote:

Pastywhyte said:
Agar can be made from the same ingredients as LC, just need to add agar. If your determined that LC can work and agar is too hard then fine. LC can work, and people can win the lottery. No one is trying to steer you wrong, we are try to help you gain success. Do a search on contamed LC and have a look at how many people felt the same as you did and learned the hard way.

Personally I would never inoculate a LC with anything but a clean agar wedge :shrug:




QFT. and why use a wedge in LC when you can go right to master jar. especially with a nice isolate :lookslucrative:

if you want to advance in this hobby just like any other hobby you need a lil cash to buy new equipment to step your game up to the next level.

which means investing in a PC for insured success in all aspects of home mycology


Edited by twistedty (12/05/13 03:33 PM)


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InvisibleDontPlay
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Re: Making LC [Re: twistedty]
    #19231922 - 12/05/13 04:00 PM (10 years, 1 month ago)

Just want to make it clear that obviously the more precautions you take using sterile techniques and a pressure cooker are most certainly going to yield better results and less problems with contamination.

But I do like to experiment and see what I can get away with going low-tek :smile: Just to prove it can be done lol.

Twistedty
"you still dont know if your LC is clean or not until you do some test jars which will take another 2-4weeks."

You are right about that, I will post my results when I make a few test jars. Worse case scenerio it doesn't work out no harm no foul.


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Edited by DontPlay (12/05/13 04:03 PM)


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InvisiblePastywhyteMDiscord
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Re: Making LC [Re: twistedty]
    #19233002 - 12/05/13 05:43 PM (10 years, 1 month ago)

Quote:

twistedty said:
QFT. and why use a wedge in LC when you can go right to master jar. especially with a nice isolate :lookslucrative:

if you want to advance in this hobby just like any other hobby you need a lil cash to buy new equipment to step your game up to the next level.

which means investing in a PC for insured success in all aspects of home mycology




Agreed. LC for cubensis is pretty much pointless.

OP the reason people assume that you have a PC is because if your planning to use advanced culturing techniques, you better get one. Even if you just want do get a LC for use in cakes, you need to step your game up. The equipment can be a little pricey when just starting, but once you have that equipment just growing is pretty cheap. Not too mention there are cheap ways to find things like a PC if you search around, it don't have to be new or the best.

As for making LC with steaming and spores, you can sometimes luck out and steam an LC successfully. Sometimes you can luck out inoculating LC with spores and tissue. The success rate for one of those practices is usually a coin flip. To do both has an extremely low chance of success tho IMO.


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InvisibleDirtygoat
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Re: Making LC [Re: Pastywhyte]
    #19234438 - 12/05/13 11:05 PM (10 years, 1 month ago)

Thanks guys.. And I know, I know lol, like I said I will buy a PC eventually lol, I just have to be sure this is a hobby I can continue, not just a one time batch thing.

And I read somewhere that as soon as you get a spore syringe, it would be a good idea to make LC from the start so that the single syringe lasts you longer.. I already used up two syringes, and I hope to successfully make some spore prints, but in case I don't, I was looking for an easy way out to increase the amount I can inoculate with the last two cc's of spore solution I have.

Again, thanks everyone, you all more than answered my first few questions.


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Offlinetwistedty
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Re: Making LC [Re: Dirtygoat]
    #19235084 - 12/06/13 03:39 AM (10 years, 1 month ago)

Quote:

Dirtygoat said:
Thanks guys.. And I know, I know lol, like I said I will buy a PC eventually lol, I just have to be sure this is a hobby I can continue, not just a one time batch thing.

And I read somewhere that as soon as you get a spore syringe, it would be a good idea to make LC from the start so that the single syringe lasts you longer.. I already used up two syringes, and I hope to successfully make some spore prints, but in case I don't, I was looking for an easy way out to increase the amount I can inoculate with the last two cc's of spore solution I have.

Again, thanks everyone, you all more than answered my first few questions.




you must of read an OLD POST saying making an LC out of a MS syringe.

buying a PC steps your mycology to a whole new level period.

unless you just want to do brf ms cakes your whole mycological career.

even RR says LC is for noobs.

once you learn brf cakes either youre happy with that and thats all you want to do, or you step your game up to agar.

its up to you and how many fruits you want to produce


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Offlinetwistedty
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Re: Making LC [Re: Dirtygoat]
    #19235091 - 12/06/13 03:44 AM (10 years, 1 month ago)

Quote:

Dirtygoat said:
Does agar need a PC?




yes. if you dont have a pc dont even bother worrying about agar just stick with making your cakes only.

there is nothing to advance to if your cant afford a pc.

just do cakes


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Re: Making LC [Re: twistedty]
    #19235093 - 12/06/13 03:46 AM (10 years, 1 month ago)

this is a hobby just like any other and requires money to advance.  i dont mean to sound like a dick sorry


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Re: Making LC [Re: JohnnieYen]
    #26494544 - 02/20/20 07:21 AM (3 years, 11 months ago)

thank you


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Offlinepoopymannn
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Re: Making LC [Re: kushman88]
    #26494564 - 02/20/20 07:31 AM (3 years, 11 months ago)

yup


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Offlinepoopymannn
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Re: Making LC [Re: twistedty]
    #26494568 - 02/20/20 07:32 AM (3 years, 11 months ago)

yeah


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Offlinepoopymannn
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Re: Making LC [Re: poopymannn]
    #26494570 - 02/20/20 07:33 AM (3 years, 11 months ago)

yup


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Offlinepoopymannn
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Re: Making LC [Re: poopymannn]
    #26494572 - 02/20/20 07:34 AM (3 years, 11 months ago)

yeah


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Offlinepoopymannn
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Re: Making LC [Re: poopymannn]
    #26494573 - 02/20/20 07:35 AM (3 years, 11 months ago)

yes


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