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skidrow
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Registered: 12/02/13
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Last seen: 10 years, 1 month
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Syringe flaming and BRF / Verm ratio
#19230458 - 12/05/13 09:29 AM (10 years, 1 month ago) |
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Hi again,
Just a simple couple of things this time.
How long do you wait before injection after flaming your syringe red hot. I'm worried if I don't wait long enough the liquid spores are going to get fried. Leave it too long and contams will settle on the syringe.
Also, does the BRF / Verm ratio have to be absolutely perfect? I might have added a bit too much BRF to my latest jar sub.
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Icesyn
Trust My Words


Registered: 05/19/11
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Re: Syringe flaming and BRF / Verm ratio [Re: skidrow]
#19230473 - 12/05/13 09:36 AM (10 years, 1 month ago) |
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Too much brf means more nutrients and most likely an off moisture balance. Gotta measure your ingredients out precisely. Keep an eye on those, they're prone to contaminate.
Tbh, I stopped flame sterilizing years ago (alcohol wipe only) and haven't come across anything I could blame on cross contamination. Run of luck perhaps.
So flame sterilize and don't worry about killing spore. There's more in there than you think. As long as you shook it good prior to inoculating!
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tripdawg420
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Re: Syringe flaming and BRF / Verm ratio [Re: skidrow]
#19230476 - 12/05/13 09:37 AM (10 years, 1 month ago) |
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squirt a few drops thru the needle too cool it
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TranscendingLife
I Don't Need a Life to Live



Registered: 06/09/10
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Re: Syringe flaming and BRF / Verm ratio [Re: skidrow]
#19230481 - 12/05/13 09:38 AM (10 years, 1 month ago) |
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The first few drops from the syringe will cool it enough that you don't have to worry about it.
They should be as close to 1:1 as possible, don't worry if you get a bit too much BRF or verm. They'll be fine.
-------------------- AMU: We Quickly Answer Questions Here "One must accept the probability of failure to experience the elation of success." - TranscendingLife “A man of genius makes no mistakes; his errors are volitional and are the portals of discovery.” - James Joyce
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thedude0345
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Re: Syringe flaming and BRF / Verm ratio [Re: skidrow]
#19231334 - 12/05/13 01:47 PM (10 years, 1 month ago) |
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started my first project about 2.5 weeks ago and i wiped with alcohol after innoculating a jar to get visible crap off the needle and then flame sterilized until red hot. held the syring upright and just squirted a few tiny drops out to cool the needle. you will be able to hear the needle sizzle as the cool spore solution runs through it. inoculated 24 brf jars and not 1 contam yet with just about 100% colonization in all my jars so far so that method seemed to work well for me!
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bodhisatta 
Smurf real estate agent



Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Syringe flaming and BRF / Verm ratio [Re: Icesyn]
#19231375 - 12/05/13 01:55 PM (10 years, 1 month ago) |
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Quote:
Icesyn said: Tbh, I stopped flame sterilizing years ago (alcohol wipe only) and haven't come across anything I could blame on cross contamination. Run of luck perhaps.
alcohol is a sanitizer and we aim for sterility. alcohol doesn't kill most spores and when it does it's not immediately. spores are what we worry about just as much as bacteria.
Flame A couple drops come out it's cool Inoculate
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skidrow
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Registered: 12/02/13
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Last seen: 10 years, 1 month
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Re: Syringe flaming and BRF / Verm ratio [Re: skidrow]
#19231408 - 12/05/13 02:03 PM (10 years, 1 month ago) |
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OK got it. One other thing, is there some sort of calculator that can tell me what % I need of either BRF or Verm. It's just that I've got a wierd glass that's like 340ml. I managed to crack it in the PC lol but not right the way through so it's ok.
The way I was doing it is adding water to the verm and mixing until a small excess or water pools. As I know 240ml verm = 40ml of BRN as per simple minds pftek, I just added an extra half a tbsp of BRM. 1 tbsp is about 18ml. Not quite lab grade for doing things, as it's bit hard to be any more precise in the kitchen. But I assume it is reasonable believe that most species are hardy enough to survive in soil with some degree of variation in nature?
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bodhisatta 
Smurf real estate agent



Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Syringe flaming and BRF / Verm ratio [Re: skidrow]
#19231416 - 12/05/13 02:06 PM (10 years, 1 month ago) |
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Quote:
skidrow said: OK got it. One other thing, is there some sort of calculator that can tell me what % I need of either BRF or Verm. It's just that I've got a wierd glass that's like 340ml. I managed to crack it in the PC lol but not right the way through so it's ok.
The way I was doing it is adding water to the verm and mixing until a small excess or water pools. As I know 240ml verm = 40ml of BRN as per simple minds pftek, I just added an extra half a tbsp of BRM. 1 tbsp is about 18ml. Not quite lab grade for doing things, as it's bit hard to be any more precise in the kitchen. But I assume it is reasonable believe that most species are hardy enough to survive in soil with some degree of variation in nature?
its a ratio 2:1:1 forget the numbers just use one whatever size cup of brf one whatever same size as the brf cup part water and two of those whatever size cups of verm.
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Stupendous-Yappi
Anomaly XB-311394


Registered: 09/23/13
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Re: Syringe flaming and BRF / Verm ratio [Re: skidrow]
#19232013 - 12/05/13 04:23 PM (10 years, 1 month ago) |
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Quote:
skidrow said: I managed to crack it in the PC lol but not right the way through so it's ok.
I would use another jar. The next time you try to PC, it could completely crack open now that it's lost some structural integrity.
Edited by Stupendous-Yappi (12/05/13 09:23 PM)
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Icesyn
Trust My Words


Registered: 05/19/11
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Re: Syringe flaming and BRF / Verm ratio [Re: bodhisatta]
#19233076 - 12/05/13 06:02 PM (10 years, 1 month ago) |
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Quote:
bodhisatta said:
Quote:
Icesyn said: Tbh, I stopped flame sterilizing years ago (alcohol wipe only) and haven't come across anything I could blame on cross contamination. Run of luck perhaps.
alcohol is a sanitizer and we aim for sterility. alcohol doesn't kill most spores and when it does it's not immediately. spores are what we worry about just as much as bacteria.
Flame A couple drops come out it's cool Inoculate
But how necessary is it really here? What are the chances of the needle catching something that needs to be torched?
In no way am I suggesting to skip this procedure. But I do feel an experiment on the rise.
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cronicr



Registered: 08/07/11
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Re: Syringe flaming and BRF / Verm ratio [Re: Icesyn]
#19234013 - 12/05/13 09:19 PM (10 years, 1 month ago) |
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wuts to experiment, doesn't get more sterile then red hot
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