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OfflineValyr
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Registered: 05/08/13
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Tryin bulk a fourth and perhaps final time.
    #19229742 - 12/05/13 12:53 AM (10 years, 1 month ago)

These are the five jars I have colonized. Mind tellin me what ya think? They were all ms noc ups. I did one grow from the same syringes already and it produced a fuzzy greyish mycellium that I thought might be cob but after a couple days coalesced together into thicker white mycellium strands. So Im a bit unsure of some jars that dont seem bright white to me. Theres also a tiny bit of moisture  in some spots.

These jars were inoculated with 2cc each on 11/3/13
They Received two shakes. 1 on 11-13-13 with about 20-25% colonization and again on 11-20

Jar 1

Jar 2

Jar3: This jar the bottom hasnt colonized and has sat like this for about fifteen days ever since the second shake with no growth.

Jar4

Jar5


If there good I will be using Franks Dunk and pasteurization techs with 1 brick coir, 2 quarts verm. Been having trouble with bulk lately so Im keeping the recipe as stock as possible this go.


Edited by Valyr (12/06/13 12:32 AM)


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OfflineKnownPown
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Re: Tryin bulk a fourth and perhaps final time. These jars look ok? [Re: Valyr]
    #19229754 - 12/05/13 01:00 AM (10 years, 1 month ago)

I would shake the ones with uncolonized parts, the myc should recover in 48-72 hours and then all your grains will be colonized too. Also your water content in your grains might not be right or just slow myc...but for my WBS I soak 18-24 hours, bring to a medium boil, instantly take off and drain, then stir for an 90 mins every 15. If it's still too wet I take a hair dryer to it and do a TP test to make sure the outside is dry enough. Goodluck man


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Offlinetwistedty
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Re: Tryin bulk a fourth and perhaps final time. These jars look ok? [Re: KnownPown]
    #19229757 - 12/05/13 01:02 AM (10 years, 1 month ago)

should of shook em along time ago, shake em now and give em 48 hours


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OfflineStromriderM
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Re: Tryin bulk a fourth and perhaps final time. These jars look ok? [Re: twistedty]
    #19229799 - 12/05/13 01:23 AM (10 years, 1 month ago)

Judging by what the bottom of jar 3 looks like I'd say your grains are too wet. Those should have been fully colonized a while ago


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OfflinePyrovision2103
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Re: Tryin bulk a fourth and perhaps final time. These jars look ok? [Re: Valyr]
    #19229812 - 12/05/13 01:33 AM (10 years, 1 month ago)

I remember reading in a past thread to lay the jars on their side when the bottom doesn't colonize due to too much moisture.


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Offlinetwistedty
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Re: Tryin bulk a fourth and perhaps final time. These jars look ok? [Re: Pyrovision2103]
    #19229826 - 12/05/13 01:48 AM (10 years, 1 month ago)

Quote:

Pyrovision2103 said:
I remember reading in a past thread to lay the jars on their side when the bottom doesn't colonize due to too much moisture.




better off shaking it and redistributing the excess moisture


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OfflinePyrovision2103
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Re: Tryin bulk a fourth and perhaps final time. These jars look ok? [Re: twistedty]
    #19229907 - 12/05/13 03:00 AM (10 years, 1 month ago)

Good to know! I learned something new today.


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OfflineValyr
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Registered: 05/08/13
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Re: Tryin bulk a fourth and perhaps final time. These jars look ok? [Re: Pyrovision2103]
    #19229911 - 12/05/13 03:03 AM (10 years, 1 month ago)

I actually did that lay it on it's side thing lol. It was on its side with a slightly upside down angle for about the last 7 days.

Ok thank you guys i will shake them :smile:

Update in a few days then!


Edited by Valyr (12/05/13 03:05 AM)


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OfflineValyr
Con nulla non si fa nulla.


Registered: 05/08/13
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Re: Tryin bulk a fourth and perhaps final time. These jars look ok? [Re: Valyr]
    #19234361 - 12/05/13 10:48 PM (10 years, 1 month ago)

Ok so I went out to town tonight to run errands and such and figured while I was out there Id grab the coir for this little project and guess what... The store I was getting it at isnt carrying it anymore, theyve replaced it with a more seasonal item...

Already know from previouse experience that theres no place else around that has it either. I found a deal for three bricks of eco earth (ive read this is the preferred brand by most) online but the kicker is its going to take a week or more to get to me. Its the right stuff though huh?

http://www.thatpetplace.com/eco-earth-3-brick-value-pack?gdftrk=gdfV2226_a_7c268_a_7c6967_a_7c204509&ne_ppc_id=1463&ne_key_id=26454012&gclid=CMDYkJermrsCFcFj7AodjyAAOw

So this sorta changes things... Now im thinking what I may do is try my first grain to grain transfer and use my five i just shook and transfer into 20 more jars (im assuming they looked good since everyone just said shake em and didnt mention anything funky looking lol). I usually fill my jars up to about the 24ozs mark (which the five i have are) so that would put about six ounces of colonized spawn from the masters into each new jar containing about 18ozs of new uncolonized wbs. Final result being 3 tubs, first two getting 1 brick and 7qts spawn and final getting 1qt less spawn. This really will make it a final attempt at bulk atleast for a while cause that would be all my wbs, and all my coir and im going back to brf cakes if it fails.

What do ya'all think? Is that enough colonized grain going into new jar? What should one expect for colonization times with that ratio? I know you can only give a generalization to that one cause you dont know the genetics and everything else im working with but still. Would it be a good use of my waiting time from the coir shipping lol?

Id be using this still air bag tek aswell for the transfer process:
http://www.shroomery.org/forums/showflat.php/Number/4947987#Post4947987


Edited by Valyr (12/06/13 02:44 AM)


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OfflineKnownPown
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Re: Tryin bulk a fourth and perhaps final time. These jars look ok? [Re: Valyr]
    #19234669 - 12/06/13 12:25 AM (10 years, 1 month ago)

I just did 14 g2g's today with 2 of my quart jars, I usually fill them up pretty good though. I'd say if you can't get anything off this grow though to keep tryin at bulk, you wont truly get the results you want with just pf cakes...plus all the maintenance of misting and fanning... F THAT!

Sounds like a good plan though while you wait for the coir. Stupid stores..


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OfflineValyr
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Re: Tryin bulk a fourth and perhaps final time. These jars look ok? [Re: KnownPown]
    #19254065 - 12/10/13 06:15 AM (10 years, 1 month ago)

Ok so its been five days since I gave them a shake... Here they are:



As you can see they seem to be recovering at a very slow rate. And actually once i started thinking on it, I saw a slow rate of growth from the other six quarts I did from the same syringe aswell. Both in jars and in bulk.

So needless to say ive decided i dont like these genetics and wont be g2g'ing them. Ill keep em and let them recover fully and bulk em still and update here. But as far as the other sixteen jars go that I pc'd up Ill be nocing them with 3 different varieties later today and probably start a grow log on them :smile:.


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OfflineStromriderM
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Re: Tryin bulk a fourth and perhaps final time. These jars look ok? [Re: Valyr]
    #19254073 - 12/10/13 06:19 AM (10 years, 1 month ago)

5 days? They should be recovered and fully colonized by now. Something is wrong


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InvisiblePastywhyteMDiscord
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Re: Tryin bulk a fourth and perhaps final time. These jars look ok? [Re: Stromrider]
    #19254091 - 12/10/13 06:30 AM (10 years, 1 month ago)

Hard to say for sure for all of them (pic quality) but jar 4 looks bacterial to my eye. I bet it does not recover.


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OfflineValyr
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Re: Tryin bulk a fourth and perhaps final time. These jars look ok? [Re: Stromrider]
    #19254115 - 12/10/13 06:42 AM (10 years, 1 month ago)

Ikr? I was thinking the same thing but I cant figure what.. I know the moisture on atleast one of the jars was too wet. But other than that I cant figure anything that im doing wrong. Temp is kept between 68 and 73. I dont have them in an incubator anymore as ive been told heat mats are bad so I just keep em in the room open air like. They are on a 12/12 light cycle. Though they were not originally on that. They were in darkness but again I learned that even during colonization that the myc likes a 12/12 light cycle so i switched it up.

I dont really see anything bacterial but im a newb so i could be wrong. The myc does look grey to me and sorta like cobweb but like i said earlier i ran into this on the previouse jars aswell and after a day or two the grey fuzz coalesced into strands and turned white so I dunno... Also havent noticed any "myc piss" in the jars. Maybe just weak genes? Though I did actually lose the first tub of this strain that i put to bulk to a nasty yellow bacterial infection.

And also Ive never gotten a ms noc up that ive had full colonization in a week like ive read alot of people on here get but even so this particular batch was very slow. Took over a month for the jars to get to the state they were in in the first pictures of this post.

Anyways, dont really see what else to do but to wait and see what they do lol. Like I said I dont like the genetics, very slow and very fuzzy myc. So whatever, once there colonized ill crack em and give em a sniff. If good stick in some coir/verm and be happy if i get anything at all.

Hopefully my next grow, will be better :smile:. If so im going to be making the jump into agar I think so i can start working on well performing isolates.


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InvisiblePastywhyteMDiscord
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Re: Tryin bulk a fourth and perhaps final time. These jars look ok? [Re: Valyr]
    #19254151 - 12/10/13 07:03 AM (10 years, 1 month ago)

Even ms agar is worth it to have clean inoculate. Maybe up your PC times as well. IDK where you live but if your elevation is high then longer PC times might really help get dem endospores.

Personally I have never had the fast germination/colonization with a ms syringe that many people are lucky enough to experience. Agar is a must for me. Check my sig for a cheap and easy way to do agar without ordering a thing off the net.


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OfflineValyr
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Re: Tryin bulk a fourth and perhaps final time. These jars look ok? [Re: Pastywhyte]
    #19254248 - 12/10/13 07:55 AM (10 years, 1 month ago)

Well I did a full ninety minutes on this batch of 16 jars I am nocing up today.

Thats a great tek pasty and I think it'll be the one I use for an introduction to agar :smile:. If everything works out with the sixteen I just started than next batch will be atleast once removed from a ms agar. Pick a nice speedy rhizo cut :smile:. The idea makes me drool lol.


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Offlinetwistedty
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Re: Tryin bulk a fourth and perhaps final time. These jars look ok? [Re: Valyr]
    #19255220 - 12/10/13 12:37 PM (10 years, 1 month ago)

you over cooked your grains they look all smooshy, you want your grains firm.  soak 8-16 hours in tepid water (this is my personal pref)

bring grains to boil/simmer and strain and steam dry.

your grains should roll around easily in the jars.

aim for a hydrated center and dry outside.

overcooked grains=too much moisture/too starchy/sticky=bacterial contams


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Re: Tryin bulk a fourth and perhaps final time. These jars look ok? [Re: twistedty]
    #19255233 - 12/10/13 12:40 PM (10 years, 1 month ago)

The few times I did WBS I found that I couldn't even simmer it, the millet would just explode. I usually just gave it a long soak and let it drain for a couple hours.


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Re: Tryin bulk a fourth and perhaps final time. These jars look ok? [Re: Pastywhyte]
    #19255242 - 12/10/13 12:42 PM (10 years, 1 month ago)

Quote:

Pastywhyte said:
The few times I did WBS I found that I couldn't even simmer it, the millet would just explode. I usually just gave it a long soak and let it drain for a couple hours.





NOOOOOOOOB

jkn, it took me SEVERAL times to get it just right.  im like a robot now ill never use rye again

OP if youre havin trouble with wbs try rye.


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Re: Tryin bulk a fourth and perhaps final time. These jars look ok? [Re: twistedty]
    #19255264 - 12/10/13 12:46 PM (10 years, 1 month ago)

Rye is sooo easy even my wife can prep it :laugh2:


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