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Offlinecleaven
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Registered: 01/25/13
Posts: 5
Last seen: 10 years, 1 month
Spore Print to Myc
    #19227379 - 12/04/13 03:45 PM (10 years, 1 month ago)

So,
I have some prints on the way in the mail.
What is the most fool proof way to turn these prints into mycellium?
Make a syringe? Use that syringe to make a liquid culture? Use it to straight up impregnate some jars?

Scrape the spores directly into a liquid culture?
Scrape the spores directly onto some agar?

It seems like agar is the preferred method..or am I wrong?
If I get petri dishes and use agar, where can I store them?


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OfflinecronicrFacebook
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Registered: 08/07/11
Posts: 61,436
Loc: Van Isle Flag
Last seen: 2 years, 8 days
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Re: Spore Print to Myc [Re: cleaven]
    #19227393 - 12/04/13 03:47 PM (10 years, 1 month ago)

agar will always be the recommendation around here:thumbup:


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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Offlinefatflash
Commodore


Registered: 01/15/13
Posts: 369
Last seen: 21 days, 15 hours
Re: Spore Print to Myc [Re: cronicr]
    #19227445 - 12/04/13 03:56 PM (10 years, 1 month ago)

Depends on what's your doing


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OfflinecronicrFacebook
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Re: Spore Print to Myc [Re: fatflash]
    #19227483 - 12/04/13 04:01 PM (10 years, 1 month ago)

Quote:

fatflash said:
Depends on what's your doing



even if you were doing a lc you should start with agar, you can make syringes but i would suggest agar ... i can't think of any situation where i would reccomend against it:shrug:
maybe you know something i don't


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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Offlinefatflash
Commodore


Registered: 01/15/13
Posts: 369
Last seen: 21 days, 15 hours
Re: Spore Print to Myc [Re: cronicr]
    #19227629 - 12/04/13 04:30 PM (10 years, 1 month ago)

From the OP its sounds like he shoulda just got a syringe save a step


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Invisiblepoopy mcpooperson
Pogonophobia
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Registered: 10/17/13
Posts: 124
Re: Spore Print to Myc [Re: fatflash]
    #19227652 - 12/04/13 04:34 PM (10 years, 1 month ago)

agar.


--------------------
love and laughter  :laugh:


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OfflineStromriderM
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Registered: 06/02/13
Posts: 7,326
Loc: Dept of know what I'm say... Flag
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Re: Spore Print to Myc [Re: poopy mcpooperson]
    #19227676 - 12/04/13 04:41 PM (10 years, 1 month ago)

Agar


Hey poopy :hi:


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OfflineDark76


Registered: 07/14/13
Posts: 121
Last seen: 5 years, 8 months
Re: Spore Print to Myc [Re: Stromrider]
    #19227771 - 12/04/13 05:06 PM (10 years, 1 month ago)

What about something like agar?  :whatdoyouthink:

:tongue:


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OfflineMoonlightblue
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Registered: 12/07/12
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Re: Spore Print to Myc [Re: Dark76]
    #19227920 - 12/04/13 05:50 PM (10 years, 1 month ago)

Agar :
150 ml tap water
3g agar
3g malt

PC for 35 mins


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Invisiblepoopy mcpooperson
Pogonophobia
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Registered: 10/17/13
Posts: 124
Re: Spore Print to Myc [Re: Stromrider]
    #19228202 - 12/04/13 06:51 PM (10 years, 1 month ago)

Quote:

Stromrider said:
Agar


Hey poopy :hi:





:wave:

hey there strom.


--------------------
love and laughter  :laugh:


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InvisibleEnigma1
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Registered: 08/15/13
Posts: 977
Re: Spore Print to Myc [Re: cleaven]
    #19228663 - 12/04/13 08:21 PM (10 years, 1 month ago)

Print >>> Syringe >>> Agar >>> Clean up >>> LC or Grain


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Offlinefractaldill
Cultivator in Training

Registered: 08/31/13
Posts: 909
Last seen: 9 years, 9 months
Re: Spore Print to Myc [Re: Enigma1]
    #19228734 - 12/04/13 08:31 PM (10 years, 1 month ago)

huh? that doesnt make sense.


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OfflineStromriderM
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Re: Spore Print to Myc [Re: Enigma1]
    #19228972 - 12/04/13 09:21 PM (10 years, 1 month ago)

Quote:

Enigma1 said:
Print >>> Syringe >>> Agar >>> Clean up >>> LC or Grain




Skip the syringe. That would be counterproductive


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InvisibleEnigma1
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Registered: 08/15/13
Posts: 977
Re: Spore Print to Myc [Re: Stromrider]
    #19229055 - 12/04/13 09:36 PM (10 years, 1 month ago)

Quote:

Stromrider said:
Quote:

Enigma1 said:
Print >>> Syringe >>> Agar >>> Clean up >>> LC or Grain




Skip the syringe. That would be counterproductive





K so just use I-loop scrape some of the print off ?

Do you dab the loop in sterilized water first ?


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Offlinefractaldill
Cultivator in Training

Registered: 08/31/13
Posts: 909
Last seen: 9 years, 9 months
Re: Spore Print to Myc [Re: Enigma1]
    #19229322 - 12/04/13 10:43 PM (10 years, 1 month ago)

Quote:

Enigma1 said:
Quote:

Stromrider said:
Quote:

Enigma1 said:
Print >>> Syringe >>> Agar >>> Clean up >>> LC or Grain




Skip the syringe. That would be counterproductive





K so just use I-loop scrape some of the print off ?

Do you dab the loop in sterilized water first ?



You can use an inoculation loop or cotton tip swab.

No. They should come pre sterilized, or sterilize them in a pc yourself.  or flame it and let it cool. dont use sterilized water, thats doing no good.


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