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North Spore Shop: Injection Grain Bag, North Spore Mushroom Grow Kits & Cultivation Supplies

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Offlinefractaldill
Cultivator in Training

Registered: 08/31/13
Posts: 909
Last seen: 9 years, 10 months
GTG Sterile procedure
    #19226997 - 12/04/13 02:35 PM (10 years, 2 months ago)

When i do my transfers i remove the lid from the jar for the entire procedure. Is this a good idea or a bad idea? I feel that it allows me to work more quickly, i have only gotten one bacterial contamination from the 4 transfers or so ive done this way. Should i keep putting the lid on and off? or is it ok to keep the mouth close to top of tote in between jars?


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OfflinePussyFart
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Re: GTG Sterile procedure [Re: fractaldill]
    #19227006 - 12/04/13 02:37 PM (10 years, 2 months ago)

i KEEP THE LID OFF MY MASTER JAR ALL THE TIME WHEN i DO MY TRANSFERS...sry for caps.


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OfflineCliftonGK1
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Registered: 03/27/13
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Re: GTG Sterile procedure [Re: fractaldill]
    #19227035 - 12/04/13 02:42 PM (10 years, 2 months ago)

I do the same, and I work in a glove box which I put all my materials into and then mist saturate with 70% ispropyl before working.
I haven't had any contamination issues in going G2G, but I've done relatively few culture passages so far.
But, for having initially started by going BRF to Grains in the same box and not running into any problems (even when monitoring the original BRF jar for a few weeks afterwards) nothing is coming up contaminated.


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InvisibleTranscendingLife
I Don't Need a Life to Live
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Registered: 06/09/10
Posts: 21,627
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Re: GTG Sterile procedure [Re: PussyFart]
    #19227040 - 12/04/13 02:43 PM (10 years, 2 months ago)

I keep the lid on unless I'm pouring from my master in my SAB.
If it works, it works, bro.

Bacteria, normally, is from something you touched then it go into your jar or it's an old/worn culture.


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North Spore Shop: Injection Grain Bag, North Spore Mushroom Grow Kits & Cultivation Supplies


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