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OfflineDraenei
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Registered: 06/18/13
Posts: 223
Last seen: 6 years, 7 months
Is 8 hours nessecary stock pot to cool for inoc?
    #19226722 - 12/04/13 01:30 PM (10 years, 1 month ago)

Seems like overcool. What would be the minimum I can inoc them at?


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Offlinenewera
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Registered: 03/05/13
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Last seen: 4 years, 1 month
Re: Is 8 hours nessecary stock pot to cool for inoc? [Re: Draenei]
    #19226732 - 12/04/13 01:32 PM (10 years, 1 month ago)

y risk it, just wait:shrug:
under 90 degrees i figure would be ideal but with no way to measure...just wait


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InvisibleTranscendingLife
I Don't Need a Life to Live
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Registered: 06/09/10
Posts: 21,627
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Re: Is 8 hours nessecary stock pot to cool for inoc? [Re: newera]
    #19226766 - 12/04/13 01:42 PM (10 years, 1 month ago)

Wait until the jars are room temp to inoculate.
If you're having problems waiting for your jars to cool,
you're going to have problems waiting 3-6 weeks for full colonization & consolidation.
Patience is key in this hobby.
Do some more reading/research on here, start new projects, read a book, etc.


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"One must accept the probability of failure to experience the elation of success." - TranscendingLife
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"Your vision will become clear only when you can look into your own heart…. Who looks outside, dreams; who looks inside, awakes."- Carl Jung
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OfflineDraenei
Rising2Dreamz


Registered: 06/18/13
Posts: 223
Last seen: 6 years, 7 months
Re: Is 8 hours nessecary stock pot to cool for inoc? [Re: TranscendingLife]
    #19226774 - 12/04/13 01:43 PM (10 years, 1 month ago)

Thanks everyone, ill just wait.


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InvisibleTranscendingLife
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Re: Is 8 hours nessecary stock pot to cool for inoc? [Re: Draenei]
    #19226829 - 12/04/13 01:52 PM (10 years, 1 month ago)

Good idea, young one. :yoda:


--------------------
AMU: We Quickly Answer Questions Here
"One must accept the probability of failure to experience the elation of success." - TranscendingLife
“A man of genius makes no mistakes; his errors are volitional and are the portals of discovery.” - James Joyce
:sporedrop::sporedrop::mushroomgrow::sporedrop::sporedrop::wow:How I Do EVERYTHING:wow::sporedrop::sporedrop::mushroomgrow::sporedrop::sporedrop:
"Your vision will become clear only when you can look into your own heart…. Who looks outside, dreams; who looks inside, awakes."- Carl Jung
"Anything that can be done chemically can be done by other means."- William S. Burroughs
"You are as dead now as you will ever be" - Seth


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OfflineCliftonGK1
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Registered: 03/27/13
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Re: Is 8 hours nessecary stock pot to cool for inoc? [Re: TranscendingLife]
    #19226983 - 12/04/13 02:32 PM (10 years, 1 month ago)

I learned long ago when I was doing laboratory culture work to let culture media cool before inoculating. 
Spent a long day doing enzymatic cuts and electoporetic transduction of some cell cultures.  Got a little ahead of myself waiting for the day to end, and my broth cultures were a wee bit on the hot side from the autoclave.  Inoculated, and ruined all my day's work.

Short story shorter:  Wait your time or ruin your work.


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