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Offlineravenfeeder
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Pasteurizing bulk substrate in autoclave?
    #19225139 - 12/04/13 01:50 AM (10 years, 2 months ago)

Hi there!

I am planning on spawning 10l of grains to 16l of bulk substrate in the near future, for my 110l monotub, and seeing as it is such a large amount of substrate, would it be possible to pasteurize it, and not sterilize it, in a laboratory autoclave, if I set the temperature knob to 70°C (+-160°F) and let it go for about two hours, allowing enough time for the core of the substrate to reach the desired temperature?

Cheers:stoned:


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OfflineRogerRabbitM
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Re: Pasteurizing bulk substrate in autoclave? (moved) [Re: ravenfeeder]
    #19225468 - 12/04/13 06:31 AM (10 years, 2 months ago)

This thread was moved from Advanced Mycology.

Reason:
Not an advanced mycology topic.

The tool you use is not relevant.  What is relevant is you maintain 140F to 160F (60C to 70C) at the center of the substrate for 60 to 90 minutes.  Use a thermometer for the first few runs to establish the necessary settings, which you can then duplicate easily later without the thermometer.
RR


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Offlineravenfeeder
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Re: Pasteurizing bulk substrate in autoclave? (moved) [Re: RogerRabbit]
    #19226623 - 12/04/13 01:06 PM (10 years, 2 months ago)

Ah horse poo, that's true.

I had a feeling the autoclave set at a lower temperature would do good initially for "pasteurizing" the bulk substrate, but I reckon the continuous temperature will eventually push the core temperature of the substrate (packed loosely in qt jars)
beyond 170°F...

Pardon for starting the thread in the wrong forum:facepalm:


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