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OfflineWPC
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Registered: 12/03/13
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Last seen: 9 years, 10 months
Rye Cakes
    #19225906 - 12/04/13 09:35 AM (10 years, 1 month ago)

Can anyone tell me what I did wrong? I have eight 1/2 pint jars with rye grain in them. before putting the grain in the jars I boiled it for about 15 min. them put the grain in the jars and pressure cooked for an hour. I then injected the spores after the jars cooled down. a week later ALL the jars had green mold. did I miss something???


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OfflinePussyFart
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Re: Rye Cakes [Re: WPC]
    #19225923 - 12/04/13 09:40 AM (10 years, 1 month ago)

You should have PCed them for at least 90 minutes......

Either that or the syringe was contaminated.


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InvisibleHypnotoadCroaked
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Registered: 01/05/13
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Re: Rye Cakes [Re: WPC]
    #19225925 - 12/04/13 09:41 AM (10 years, 1 month ago)

Quote:

WPC said:
Can anyone tell me what I did wrong? I have eight 1/2 pint jars with rye grain in them. before putting the grain in the jars I boiled it for about 15 min. them put the grain in the jars and pressure cooked for an hour. I then injected the spores after the jars cooled down. a week later ALL the jars had green mold. did I miss something???





Too wet?  Improper needle sterilization?  (Do not wipe the needle after flame sterilizing).  Did you include vermiculite in your recipe?  What type of Gas exchange and inoculation ports do you use?


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OfflineWPC
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Re: Rye Cakes [Re: HypnotoadCroaked]
    #19225950 - 12/04/13 09:48 AM (10 years, 1 month ago)

They didn't look to moist to me. I sterilized the needle by alcohol swab then with a flame. did not use any verm. inoculation port was just a medium sized hole in lid of jar, also served as the air exchange with a type of fine mesh as a filter. where did I go wrong??


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Offlinedpomalia
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Re: Rye Cakes [Re: HypnotoadCroaked]
    #19225957 - 12/04/13 09:53 AM (10 years, 1 month ago)

I'd assume your innoc tek. Did u use a hood or glove box flame inbetween jars? Was it a previously Used syringe? Could be the supplier but if u used a sponser the chances are fairly slim. If u need a temp easy glove box look up still air bag or something similar it works for me every time
Only use with a new syringe though you cannot flame steralize but you won't need to your enviorment is sterile and you will have just opened the needle


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Offlinedpomalia
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Re: Rye Cakes [Re: dpomalia]
    #19225961 - 12/04/13 09:55 AM (10 years, 1 month ago)

And without Verm layer contams reach ur sub much easier so unless you have a self healing port with ge holes w micro pore tape a tight mesh filter ain't gonna cut it. May work but ur success rate will be hurtin


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OfflineWPC
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Re: Rye Cakes [Re: dpomalia]
    #19225967 - 12/04/13 09:59 AM (10 years, 1 month ago)

so should I mix the verm and the moist grain together before putting in jars or just put a top layer in the jars?


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Offlinedpomalia
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Re: Rye Cakes [Re: WPC]
    #19225982 - 12/04/13 10:05 AM (10 years, 1 month ago)

With grain you don't put Verm because you have to shake them and after shaking that layer your spreading everything it caught put gypsum to keep shakeable and so it doesn't clump just as a tip. But with grain its all about the lids and your sterile tek cause once you innoc there's nothing you can do about contams. Get silicone to cover a injection hole cause it self heals and I've had waterproof medical tape work for me a lot but my area is clean and its not reccomended but cheap and easily obtained. Pf tek is much easier to keep contam free


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Offlinedpomalia
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Re: Rye Cakes [Re: dpomalia]
    #19225988 - 12/04/13 10:08 AM (10 years, 1 month ago)

If u only have grain and don't want to get brf then u can put the verm layer on top before PC but then u cannot shake and thats the point of grain


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OfflineWPC
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Re: Rye Cakes [Re: dpomalia]
    #19225995 - 12/04/13 10:10 AM (10 years, 1 month ago)

cool, this helps a lot. where can a buy silicone from? craft store?


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Offlinedpomalia
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Re: Rye Cakes [Re: WPC]
    #19226008 - 12/04/13 10:15 AM (10 years, 1 month ago)

Walmart or similar stores its like 5 bucks for a little tube any will work but clear is the best to see ur holes I've had the problem of using black n I broke my syringe trying to get it in the hole. Takes like 3 hrs to dry u can pull it off the holes with a little heat if u want to reuse the lids for something different


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OfflineWPC
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Re: Rye Cakes [Re: dpomalia]
    #19226017 - 12/04/13 10:17 AM (10 years, 1 month ago)

perfect. thanks a bunch.


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Offlinedpomalia
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Re: Rye Cakes [Re: WPC]
    #19226036 - 12/04/13 10:26 AM (10 years, 1 month ago)

No problem
Pm me if u got a question that isn't getting answered in the posts


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InvisibleHypnotoadCroaked
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Re: Rye Cakes [Re: WPC]
    #19226052 - 12/04/13 10:34 AM (10 years, 1 month ago)

Quote:

WPC said:
They didn't look to moist to me. I sterilized the needle by alcohol swab then with a flame. did not use any verm. inoculation port was just a medium sized hole in lid of jar, also served as the air exchange with a type of fine mesh as a filter. where did I go wrong??



I dunno what type of "mesh" you are speaking of, but depending on what type of micron rating it has, it may be suspect.  You could try to stuff the hole as tight as possible with poly-fill much like a grain spawn jar. 

The rest of your methods seem correct...alcohol is worthless however especially since you are going to flame sterilize, but that should not have cause the issue.

How much inoculation are you providing to each cake?  I would think that much like a grain jar a few drops should be sufficient. 

What you are doing does not have a specific tek per se, but there are methods to success. Seems like you are very aware of methods and seem to be on the right track.


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InvisibleStool_Sample
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Re: Rye Cakes [Re: HypnotoadCroaked]
    #19226235 - 12/04/13 11:33 AM (10 years, 1 month ago)

Um, there is a lot of nonsense on this post.
I just wanted to add
to the Original OP...

Go back to the Let's Grow Mushrooms  and relearn the Proper Tek.
Keep it simple
Start on agar.
Solves problem of bad syringe / bad grain creation.

I have had many bad syringes from the Provided list of suppliers. Of course, you can say that the syringe got damaged in shipping  (because they don't sell them to be grown.!!)- and get another one from the supplier


--------------------
"wise words here"
:toadkart:


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OfflineWPC
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Re: Rye Cakes [Re: dpomalia]
    #19226399 - 12/04/13 12:16 PM (10 years, 1 month ago)

one more question, will the silicone hold up in the pc?


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OfflinePussyFart
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Re: Rye Cakes [Re: WPC]
    #19226408 - 12/04/13 12:19 PM (10 years, 1 month ago)

Quote:

WPC said:
one more question, will the silicone hold up in the pc?



Yes.....most silicone has a melting point of well above 250F......


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OfflineWPC
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Re: Rye Cakes [Re: PussyFart]
    #19226424 - 12/04/13 12:25 PM (10 years, 1 month ago)

where can I buy micro tape?


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OfflinecronicrFacebook
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Re: Rye Cakes [Re: WPC]
    #19226487 - 12/04/13 12:38 PM (10 years, 1 month ago)

same places that sell band aids


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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