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InvisibleStuey1
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Registered: 11/28/13
Posts: 740
Loc: Bat Country
Glovebox question
    #19222696 - 12/03/13 03:12 PM (10 years, 1 month ago)

While using a glovebox is it necessary to heat your syringe between every jar with a flame. It's a little difficult to use a lighter with the kitchen gloves on, and it seems a little odd to try and put a constant flame source (oil lamp etc.) in a sealed container

I was thinking of just keeping a cotton ball soaked in ISO alchahol to put the needle in between jars


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Have you ever danced with the devil in the pale moon light?


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OfflinePussyFart
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Registered: 04/08/12
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Re: Glovebox question [Re: Stuey1]
    #19222743 - 12/03/13 03:20 PM (10 years, 1 month ago)

Quote:

Stuey1 said:
While using a glovebox is it necessary to heat your syringe between every jar with a flame. It's a little difficult to use a lighter with the kitchen gloves on, and it seems a little odd to try and put a constant flame source (oil lamp etc.) in a sealed container

I was thinking of just keeping a cotton ball soaked in ISO alchahol to put the needle in between jars



Alcohol is not sterile, and it does not sterilize.....it does not kill mold spores...it is not a replacement for flame sterilizing.

Heat sterilizes.....flame the needle until red hot between jars.....do not wipe the sterile needle with alcohol.

Do not use kitchen gloves....use surgical gloves that are attached to you and not the box.

This way it's really easy top flame the needle outside the box and bring it back in.


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Offlinenn-IlliniSpiralDMT
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Registered: 07/19/12
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Re: Glovebox question [Re: PussyFart]
    #19222832 - 12/03/13 03:35 PM (10 years, 1 month ago)

Quote:

Notahacker420 said:
Quote:

Stuey1 said:
While using a glovebox is it necessary to heat your syringe between every jar with a flame. It's a little difficult to use a lighter with the kitchen gloves on, and it seems a little odd to try and put a constant flame source (oil lamp etc.) in a sealed container

I was thinking of just keeping a cotton ball soaked in ISO alchahol to put the needle in between jars



Alcohol is not sterile, and it does not sterilize.....it does not kill mold spores...it is not a replacement for flame sterilizing.

Heat sterilizes.....flame the needle until red hot between jars.....do not wipe the sterile needle with alcohol.

Do not use kitchen gloves....use surgical gloves that are attached to you and not the box.

This way it's really easy top flame the needle outside the box and bring it back in.




Jeez hacker for once just answer people's damn questions without the CHICAGO flavor on it all the time lol

OP yes it is necessary to heat sterilize the needle in between injections into each individual jar. Don't connect the gloves as they limit your movement inside the SAB and make it a hassle if you drop anything, most likely pulling in contaminated air into your work area. There are plenty of videos on youtube on how to make an alcohol lamp, itll cost you 4$ tops or you could buy one for 10$ off of ebay. Leave all flames outside of your SAB, you don't want to explode.
Never put open flames in a SAB that is sprayed with alcohol, it has fire hazard all over it. Instead just leave it outside and flame, then bring into your box.:thumbup:


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OfflinePussyFart
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Re: Glovebox question [Re: nn-IlliniSpiralDMT]
    #19222840 - 12/03/13 03:36 PM (10 years, 1 month ago)

Quote:

nn-IlliniSpiralDMT said:
Jeez hacker for once just answer people's damn questions without the CHICAGO flavor on it all the time lol



Not sure what you mean......I like my relish......


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Offlinenn-IlliniSpiralDMT
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Registered: 07/19/12
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Re: Glovebox question [Re: PussyFart]
    #19223008 - 12/03/13 04:19 PM (10 years, 1 month ago)

Quote:

Notahacker420 said:
Quote:

nn-IlliniSpiralDMT said:
Jeez hacker for once just answer people's damn questions without the CHICAGO flavor on it all the time lol



Not sure what you mean......I like my relish......




exactly... mustard>relish


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InvisibleStuey1
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Posts: 740
Loc: Bat Country
Re: Glovebox question [Re: PussyFart]
    #19223118 - 12/03/13 04:52 PM (10 years, 1 month ago)

Leaving open holes in the side of the box, doesn't that defeat the whole purpose ov the sab


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Offlinenn-IlliniSpiralDMT
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Registered: 07/19/12
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Re: Glovebox question [Re: Stuey1]
    #19223134 - 12/03/13 04:58 PM (10 years, 1 month ago)

Quote:

Stuey1 said:
Leaving open holes in the side of the box, doesn't that defeat the whole purpose ov the sab



Quote:

Stuey1 said:
Leaving open holes in the side of the box, doesn't that defeat the whole purpose ov the sab




One would think that lol that's what I thought too:curbyourenthusiasm:

But honestly it is way easier to work in SAB with the gloves attached to you and tyvek sleeves over your gloves. It is so much easier and I regret taking so long to spend 20$ on an alcohol lamp, scalpel, and tyvek sleeves. IDK what the hell I was thinking. The goal of a SAB is to keep the air still not completely sterile, just still. You'll be fine leaving one arm hole open. Keep the lamp right next to you and flame your tools till they are red hot and go back in to work:thumbup:


Sleeves, lamp, scalpel... you won't regret it.


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InvisibleStuey1
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Registered: 11/28/13
Posts: 740
Loc: Bat Country
Re: Glovebox question [Re: nn-IlliniSpiralDMT]
    #19223159 - 12/03/13 05:04 PM (10 years, 1 month ago)

Makes sense. I get a little OCD about being clean. I'll leave open holes.


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Offlinenn-IlliniSpiralDMT
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Registered: 07/19/12
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Re: Glovebox question [Re: Stuey1]
    #19223184 - 12/03/13 05:10 PM (10 years, 1 month ago)

Quote:

Stuey1 said:
Makes sense. I get a little OCD about being clean. I'll leave open holes.




Oh me too, even if I was in a clean room and knew everything was sterile, I'd still be running around spraying shit with oust and iso:thumbup:

I feel like Monk sometimes lol


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InvisibleHypnotoadCroaked
Retired, but will check MSGs

Registered: 01/05/13
Posts: 1,168
Re: Glovebox question [Re: Stuey1]
    #19223189 - 12/03/13 05:12 PM (10 years, 1 month ago)

Quote:

Stuey1 said:
Leaving open holes in the side of the box, doesn't that defeat the whole purpose ov the sab



Quote:

Stuey1 said:
Makes sense. I get a little OCD about being clean. I'll leave open holes.



I use 4 1/2" holes in my sab, and no my arms are not that big.  I have had 0 problems with contamination since I switched to using the sab.  I highly advise anyone who has any desire for success to consider getting at least a SAB.  A flow hood would also work, but seeing how it can cost $500+ more than a tote with a few holes in it, its almost a no-brainer.

TrancendingLife has a great writeup on his SAB.

http://www.shroomery.org/forums/showflat.php/Number/14722698#14722698

Good luck!


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