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trippyshroom
Bacterial genius



Registered: 05/07/13
Posts: 363
Last seen: 4 months, 17 days
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wbs question
#19221495 - 12/03/13 10:46 AM (10 years, 2 months ago) |
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hey shroomers,
im trying out wbs for the first time and i ahve been searching for a few days and i havent found an answer i have a 2 syringes one cambodian and one z strain and i was wondering if i can do the 2 strains in one 18 gal monotub?
Thanks, Trippy
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dceodhz
Up And Coming

Registered: 10/19/13
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Didn't Mean STRAIN as if it were a different SPECIES....
Edited by dceodhz (12/03/13 12:18 PM)
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bodhisatta 
Smurf real estate agent



Registered: 04/30/13
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Loc: Milky way
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yes, there not strains. They're both the species P.cubensis. They will work together. Two hyphae meet and become dikaryotic mycelium that's a strain, you'll have 1000's of strains in your monotub if you start from spores. Each strain with its own host of traits.
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bodhisatta 
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Registered: 04/30/13
Posts: 61,890
Loc: Milky way
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Re: wbs question [Re: dceodhz]
#19221512 - 12/03/13 10:50 AM (10 years, 2 months ago) |
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dceodhz said: You should NOT introduce 2 separate strains into the same substrate.
No nothing bad will happen but it's kind of like what's the point, more than likely one strain will take over and you won't get to see the different strains.
Try one strain first in the monotub and then the second strain after your first grow is complete.
This is complete bullshit
the "vendors name/variety" will not fight each other.
Edited by Trusted cuItivator (12/03/13 10:51 AM)
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trippyshroom
Bacterial genius



Registered: 05/07/13
Posts: 363
Last seen: 4 months, 17 days
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so should i do it or not?
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bodhisatta 
Smurf real estate agent



Registered: 04/30/13
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Loc: Milky way
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Quote:
trippyshroom said: so should i do it or not?
go for it if you want to. There's really no reason to do it, or for that matter, not to do it.
I personally would use 1 small drop of spores put it to agar put it to grain then g2g the grain out and have only used a small drop of spores to do the whole monotub, but that's just one of the better ways, not the only way.
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SamhainJ
I wanna rock out, in my dreams
Registered: 05/16/04
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Back in the day people always said that if you innoc with two diff strains that one would dominate the mycelial network.. but what the hell do i know all my info is from 6 or 7 years ago haha
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trippyshroom
Bacterial genius



Registered: 05/07/13
Posts: 363
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well i dont have any agar yet kinda broke atm. maybe use .5 ml per quart jar cause i dont have alot of spores left
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bodhisatta 
Smurf real estate agent



Registered: 04/30/13
Posts: 61,890
Loc: Milky way
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Also this isn't really a WBS question ha,
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trippyshroom said: i ahve been searching for a few days and i havent found an answer
I'm sorry your searching skills are that of a second grader I literally found PAGES on my first search answering this question
The first link in my signature goes into how to get the most out of the search engine, you should have a read.
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Fahkface said:
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Satori23 said: There is no point in knocking up one jar with two strains. Only one will fruit.. if any.
Although there is indeed no bigger point in doing it, it won't result in only one of the "names" to grow.
In fact it doesn't matter at all. It's the same species of mushrooms and the name someone written on a syringe or a print has next to nothing to with what comes out in the end.
As with any multispore knock up, compatible hyphae will combine, while incompatible ones will grow out individually. This happens just as often as with a multispore knock up from a single print. You often have several different strains growing side by side, which often results, in slightly different fruit shapes and growing habits. Same thing with knocking a jar up with two spore prints of different names. It doesn't matter. The spores won't think: "Oh no! That's B+! Now we have to fight!". As long it's the same species they won't give a shit if it's named "B+" or "Bill Murray".
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RogerRabbit said:
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Cervantes said: They DO compete or people wouldn't isolate strains. 
Not exactly, and that isn't why we isolate strains.
The same thing happens every time you use multispore inoculation, which could just as accurately be called multi-strain inoculation. They don't compete any more than the thousands of hyphae that emerge from an inoculation site. I've done this hundreds of times.
Try it different ways just for grins and giggles. Inoculate two holes of a jar with one strain and the other two holes with another. Or, inoculate each of the 4 holes with a separate syringe(strain), for four strains per jar. You can mix them at spawning or mix them at inoculation. Do a few each way. I doubt you'll be able to tell any difference between them all, at least no more than you'd normally see in a multispore grow. RR
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RogerRabbit said: Strains of the same species cross easily. I avoid calling them 'hybrids', especially since the only difference many of the strains have is the name. Every time you use multispore inoculation, thousands of 'strains' are created. Most of these combine into a single organism. Whether the spores came from the same print or multiple prints is irrelevant. RR
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RogerRabbit said: This thread has been closed.
Reason: A cube is a cube and there's virtually no difference between any of them, and certainly no difference in how you grow them.
Every flush is different, thus new folks often think that difference is strain related, when in fact you could do many grows from the same syringe/strain and they'd all be different. That's the nature of fungal genetics, and why you never see the old hands arguing about 'strains' or even discussing which one is better. Please use the strain thread stickied at the top for discussion. Thanks. RR
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RogerRabbit said:
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FuckMeRunnin said: Actually when you fruit two different cube variations in the same tub you don't get a hybrid. The mycelium doesn't come together and create a hybrid, it grows along side one another and you will end up with some TC and some GT. So sorry mustard tiger it was all in your head.
Even if two strains crossed, which they often do, it wouldn't be a hybrid. If a lady named Smith and a man named Jones had a baby, would you call it a hybrid? No. Taken farther, if a man from Japan and a woman from Africa had a baby, would it be a hybrid? No, it would still be the same species, human.
There are no sub-strains. A strain is a strain, period. A strain is a pairing of compatible hyphae, not the name some idiot wrote on a print, the spores of which can create tens of thousands of individual strains. When people get a print and call it a 'strain' I go batshit, because it only serves to spread disinformation.
This is why edibles are only sold as strains when an isolated single sector of mycelium is traded. If you fruit a shiitake for example and take a sporeprint, your original strain is gone once you start growing from spores again. RR
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trippyshroom
Bacterial genius



Registered: 05/07/13
Posts: 363
Last seen: 4 months, 17 days
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wow many thanks and sorry for my searching skills i will deffinetly read up on that.
-------------------- Anything that is posted above is just a figment of your imagination.
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SamhainJ
I wanna rock out, in my dreams
Registered: 05/16/04
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Haha i didnt even realize that the op had pretty much nothing to do with wbs, its more of a strain discussion thread.. i bet this gets closed
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bodhisatta 
Smurf real estate agent



Registered: 04/30/13
Posts: 61,890
Loc: Milky way
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Quote:
trippyshroom said: wow many thanks and sorry for my searching skills i will deffinetly read up on that.
you can limit the age of posts to 2 years or newer, and replies only from trusted cultivators, etc... I used the search term "two strains" and trusted cultivators box checked. There's even better ways to tweak that search but even that provided all the info I needed.
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