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Invisiblemaddchef
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Wierd contam issues lately
    #19219389 - 12/02/13 08:48 PM (10 years, 1 month ago)

So I've been having tubs go bad on me lately before the first flush. My spawn is what's troubling me. Ya see I shake a day or two before spawning and they recover fine but when I open my jars there's an unmistakable minty kinda smell of trich, and sure enough that's been what's been plaguing my tubs.

I opened a colonized jar as an experiment, let some fresh air in trying to get whatever contam to sporelate and sealed it back up for about 3 weeks, shook it and it recovered fine. No signs of anything except myc.

Obviously I'm going to have to go back a few steps to a clean culture but has anyone encountered such?


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In the land of the blind, the one eyed man is king.

All mushrooms are edible, but some only once.....                     

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InvisibleHypnotoadCroaked
Retired, but will check MSGs

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Re: Wierd contam issues lately [Re: maddchef]
    #19219468 - 12/02/13 09:11 PM (10 years, 1 month ago)

Quote:

maddchef said:
has anyone encountered such?



Yep.  Last summer everything I did failed @ first flush or sooner.  This spurned me into agar, where I found out that my MS provided by a trusted vendor was not 100% clean.  A few xfers later I was back to square one.  I managed a few smaller mono tubs during that time, which granted me prints for future work. 

In the event that catastrophe happens, I fall back to trying to obtain a print.  If not possible, than off to the compost bin. 

I feel your pain totally, and good luck.


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Offlinetwistedty
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Re: Wierd contam issues lately [Re: HypnotoadCroaked]
    #19219501 - 12/02/13 09:19 PM (10 years, 1 month ago)

this happend to me and it was my pasteurization process.  what tek are you using for your pasteurization of your and what is your substrate


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Invisiblemaddchef
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Re: Wierd contam issues lately [Re: twistedty]
    #19219995 - 12/02/13 10:53 PM (10 years, 1 month ago)

Stovetop proper pasteurization and I'm using coir/verm. I believe my long run of mishaps is from G2G of the invisible critter but for the life of me I've never seen jars recover and never show signs of contam like this


--------------------
In the land of the blind, the one eyed man is king.

All mushrooms are edible, but some only once.....                     

                                        Easier than cakes

I do science and shit.

:havesomescience:


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InvisibleFrankHorrigan
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Re: Wierd contam issues lately [Re: maddchef]
    #19220110 - 12/02/13 11:24 PM (10 years, 1 month ago)

If you are properly pasteurizing coir and verm, no doubt the contam is in your spawn.

Go back to your cultures, get some clean plates before you inoculate your next set of jars.


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OfflineKizzle
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Re: Wierd contam issues lately [Re: FrankHorrigan]
    #19221691 - 12/03/13 11:35 AM (10 years, 1 month ago)

Trich can be really sneaky like that, especially when g2g are involved. If you can smell it in your spawn jars then I have no doubt that's the source.

You have to bare in mind just because a mold isn't sporulating in a grain jar doesn't mean it won't sporulate after you spawn it, they're different substrates and they're under different conditions.


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InvisibleFrankHorrigan
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Re: Wierd contam issues lately [Re: Kizzle]
    #19221709 - 12/03/13 11:39 AM (10 years, 1 month ago)

You may be referring to green molds in general as "Trich."

Trich isn't very sneaky at all, it is so fast and fungicidal that it's usually apparent when it is present.

However, other green molds can be really sneaky, I had an issue with mold enmeshed with my cultures on agar, growing basically in tandem.  The green would never show up until 2-3 days after the first flush.


--------------------

Yes, you can bump my old threads with a question.
Here is how I get things done.
You should take a look. :hehehe:


Frank's tips and tricks. Updated on 3/21/14
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OfflineKizzle
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Re: Wierd contam issues lately [Re: FrankHorrigan]
    #19221747 - 12/03/13 11:50 AM (10 years, 1 month ago)

I mean Trichoderma specifically though it's not sneaky on agar, no. In comparison though in a spawn jar Pennicillium and Aspergillus spp. usually seem to start sporulating during spawn colonization.

Something I actually learned recently is there's a difference in smell in different species of Trich. Only T. Atrovirde and some strains of T. Virde will have the unique "minty" type smell with other species having a more general dirt-like mold odor.

And Frank I actually didn't realize this until you mentioned it before, that I can't remember ever see actual Trich, or rather any green mold with the same kind thick mycelial mat, sporulate inside a spawn jar.


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Invisiblemaddchef
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Re: Wierd contam issues lately [Re: Kizzle]
    #19223262 - 12/03/13 05:32 PM (10 years, 1 month ago)

That's why I opened a jar to introduce some fae and see if I could get it to sporelate. Although the repeated recovery after shaking still has me stumped.


--------------------
In the land of the blind, the one eyed man is king.

All mushrooms are edible, but some only once.....                     

                                        Easier than cakes

I do science and shit.

:havesomescience:


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Offlinetwistedty
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Re: Wierd contam issues lately [Re: FrankHorrigan]
    #19223309 - 12/03/13 05:47 PM (10 years, 1 month ago)

Quote:

FrankHorrigan said:
You may be referring to green molds in general as "Trich."

Trich isn't very sneaky at all, it is so fast and fungicidal that it's usually apparent when it is present.

However, other green molds can be really sneaky, I had an issue with mold enmeshed with my cultures on agar, growing basically in tandem.  The green would never show up until 2-3 days after the first flush.




same here and it didnt seem that invasive compared to trich and i was able to pull the first flush without any problems


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OfflineKizzle
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Re: Wierd contam issues lately [Re: maddchef]
    #19224849 - 12/03/13 11:03 PM (10 years, 1 month ago)

Quote:

maddchef said:
That's why I opened a jar to introduce some fae and see if I could get it to sporelate. Although the repeated recovery after shaking still has me stumped.



I know of 2 thing that can trigger sporulation in Trich. Light, especially in the blue-UVA range and nutrient stress. Fresh air may not even be a trigger for it.


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