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cacophonyhead
Shoshin



Registered: 01/30/13
Posts: 93
Loc: Canada
Last seen: 8 years, 1 month
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New question for "blobs"
#19217624 - 12/02/13 02:50 PM (10 years, 1 month ago) |
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I am hoping some of the more advanced cultivators can chime in on this one; but I do appreciate anyones theory... I have read a lot on blobs and still cant seem to figure out what went wrong here. I took 3 tissue samples from a fruit of the EQ variety. I feel my extraction was solid using a SAB, Iodine, tearing the fruit open, Flaming the exacto. Tissue samples went to agar. Agar to grain and followed with g2g transfers. 2 monotubs are coir/verm. 1 tub is hpoo/verm/worm casting. All tubs are blobs that look like large AA+ pins (large blob bodies with off white undeveloped caps). 1 tub was cleaned with water and lysol. 2 tubs cleaned with water and rubbing alcohol. Im on second flush and it is still wall to wall blobs. Not one regular fruit. Thanks for reading. Ideas?
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In the beginners mind there are many possibilities, in the expert mind there are few
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Tmethyl
Smear in the shale


Registered: 07/16/12
Posts: 16,431
Loc: Florida
Last seen: 1 year, 4 months
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Personally I've never seen a second flush be all blobs, nearly every first flush does, for me, however. Can you compose a photo of the second flush?
Typically the blobs develop more prolifically on heavily nutritious substrates, such as with horse manure, or horse manure mixtures, or with 50/50 spawn/sub ratios(the spawn is highly nutritious), but even with these mixtures the second flush is normal(due to the substrate losing most of it's nutritional value). In experimentation I noticed that 100% straw substrates didn't blob, at all, even on first flush, maybe a couple sometimes, but nothing worth noting.
I think that the blobs development is substrates associated, and have eliminated most if not all other hypothesis.
A photo of your second flush would help, I find this strange.
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cacophonyhead
Shoshin



Registered: 01/30/13
Posts: 93
Loc: Canada
Last seen: 8 years, 1 month
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Re: New question for "blobs" [Re: Tmethyl]
#19218069 - 12/02/13 04:31 PM (10 years, 1 month ago) |
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Thanks for reading Tmethyl. You get them almost every first flush you say? I find that odd in my experience, for I have only experienced them a hand full of times in the past few years. I understand the factor could be the sub being overly nutrient; however, only 1 tub is hpoo. 2 tubs are only a brick of coir and 3qt fine verm. As for ratio, its the same I always use. Sub lays 3 inches high in a 66qt tub. Spawn is usually 10 - 12 500ml jars of rye or wheat. Im thinking maybe the problem could be somewhere in the tissue sample? I do agar work, but usually only with prints. Could it be my cloning technique? I'll post a pic of second flush in 6 hours or so. Thanks again.
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In the beginners mind there are many possibilities, in the expert mind there are few
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PirateSwazey



Registered: 12/12/12
Posts: 2,993
Loc: Here, Now
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I always thought blobs came from mutated genetics. 
Strange that you got a bunch of mutants from a clone, how far did you isolate?
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StygianKnight
A Mushroom

Registered: 03/12/12
Posts: 2,717
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I've seen second flush blobs but only on PE. If this isn't an isolate you might be seeing two first flush blob sets of similar genetics.
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cacophonyhead
Shoshin



Registered: 01/30/13
Posts: 93
Loc: Canada
Last seen: 8 years, 1 month
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Hmmm. Ok, it's good you both mentioned isolation. I skipped that with these 3 tissue samples. I read a post a little while back where someone mentioned isolation wasnt 100% positive since a healthy fruit requires the multiple genetics. It seemed like whatever it was that I read at the time was a pretty solid theory so I purposely did straight from 1st agar to grain to see if there is much difference in size and sub coverage of these fruits in comparison to a regular ol MSI run. You think thats it? Thanks for the ideas everyone!
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In the beginners mind there are many possibilities, in the expert mind there are few
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PirateSwazey



Registered: 12/12/12
Posts: 2,993
Loc: Here, Now
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Could be.
What I've been doing is taking 2 wedges from a dish...
Transfer 1 to a new petri to furthur isolate.
Transfer the other to a jar to be a g2g master.
This is to test viability at different levels of isolation.
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cacophonyhead
Shoshin



Registered: 01/30/13
Posts: 93
Loc: Canada
Last seen: 8 years, 1 month
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I hear ya. I do similar but cut wedges into 4 parts and transfer into new agar and continue. Never tried first agar to grain so maybe thats the issue. I'll post a pic of 2nd flush tonight anyhow...
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In the beginners mind there are many possibilities, in the expert mind there are few
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cacophonyhead
Shoshin



Registered: 01/30/13
Posts: 93
Loc: Canada
Last seen: 8 years, 1 month
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A close up and a full shot of a second flush. This sub is a coir/verm.
[image] [/image]
[Image] [/image]
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In the beginners mind there are many possibilities, in the expert mind there are few
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Josh.0
ConnoissurOfSorts


Registered: 11/25/13
Posts: 553
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cacophonyhead
Shoshin



Registered: 01/30/13
Posts: 93
Loc: Canada
Last seen: 8 years, 1 month
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Re: New question for "blobs" [Re: Josh.0]
#19220729 - 12/03/13 05:32 AM (10 years, 1 month ago) |
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↑ Haha! Indeed. Mindblowing.
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In the beginners mind there are many possibilities, in the expert mind there are few
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cacophonyhead
Shoshin



Registered: 01/30/13
Posts: 93
Loc: Canada
Last seen: 8 years, 1 month
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Re: New question for "blobs" [Re: Tmethyl]
#19224449 - 12/03/13 09:27 PM (10 years, 1 month ago) |
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If you have any further idea's Tmethyl, I'd love to hear them.
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In the beginners mind there are many possibilities, in the expert mind there are few
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cacophonyhead
Shoshin



Registered: 01/30/13
Posts: 93
Loc: Canada
Last seen: 8 years, 1 month
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Re: New question for "blobs" [Re: Tmethyl]
#19224456 - 12/03/13 09:31 PM (10 years, 1 month ago) |
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If you have any further idea's Tmethyl, I'd love to hear them.
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In the beginners mind there are many possibilities, in the expert mind there are few
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StygianKnight
A Mushroom

Registered: 03/12/12
Posts: 2,717
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Those look like mutants to me. In which case all of that genes flushes will look similar to that. Your fruit body's genes are either hiding in the sub waiting to fruit their turn or was on a different section of your agar.
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cacophonyhead
Shoshin



Registered: 01/30/13
Posts: 93
Loc: Canada
Last seen: 8 years, 1 month
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Im good with that theory
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In the beginners mind there are many possibilities, in the expert mind there are few
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