|
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
|
KingCloyster
Forest Mushroom 🍄



Registered: 05/27/13
Posts: 597
Loc: Quetico, Canada
Last seen: 1 year, 17 days
|
Accidentally went up to 24 psi when making slants with popsicle sticks... Out of agar now.
#19217274 - 12/02/13 01:24 PM (10 years, 1 month ago) |
|
|
I used the last of my LME on 6 slant tubes with water-soaked popsicle sticks in them.
But I was busy with an agar>grain transfer that wasnt going my way [Fuckin agar wouldnt drop off my scalpel when trying to transfer X(] & ended up completely spacing the Sterilizer was still going and it went up to 23-24 psi for like 5 minutes.
Am I screwed? I cant re do it cuz im out of agar. but I fear the agar may have carmelized or i might have changed the properties of the wood stick.
Its cooling right now.
Edited by KingCloyster (12/02/13 01:25 PM)
|
eLeSDenes
Mycelium Expander




Registered: 05/25/11
Posts: 955
Last seen: 1 year, 11 months
|
Re: Accidentally went up to 24 psi when making slants with popsicle sticks... Out of agar now. [Re: KingCloyster]
#19217299 - 12/02/13 01:32 PM (10 years, 1 month ago) |
|
|
So what do you want to hear?you'll probably find it out yourself in about 3 hours. Agar supplies are chep. Shit happens. Don't panic
|
KingCloyster
Forest Mushroom 🍄



Registered: 05/27/13
Posts: 597
Loc: Quetico, Canada
Last seen: 1 year, 17 days
|
Re: Accidentally went up to 24 psi when making slants with popsicle sticks... Out of agar now. [Re: eLeSDenes]
#19217309 - 12/02/13 01:35 PM (10 years, 1 month ago) |
|
|
They are hard to find in my area... I just want to know if going up to 24 psi for a bit would ruin a slant with LMEA and a popsicle stick
I wouldnt want to lose any isolates
|
RogerRabbit
Bans for Pleasure



Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 11 months, 4 days
|
Re: Accidentally went up to 24 psi when making slants with popsicle sticks... Out of agar now. [Re: KingCloyster]
#19217318 - 12/02/13 01:38 PM (10 years, 1 month ago) |
|
|
24 psi didn't caramelize the agar. I would be surprised if it didn't boil over though. If the slant and agar is intact, it's OK. RR
-------------------- Download Let's Grow Mushrooms semper in excretia sumus solim profundum variat "I've never had a failed experiment. I've only discovered 10,000 methods which do not work." Thomas Edison
|
KingCloyster
Forest Mushroom 🍄



Registered: 05/27/13
Posts: 597
Loc: Quetico, Canada
Last seen: 1 year, 17 days
|
Re: Accidentally went up to 24 psi when making slants with popsicle sticks... Out of agar now. [Re: RogerRabbit]
#19217343 - 12/02/13 01:43 PM (10 years, 1 month ago) |
|
|
We'll see in about 20 minutes and ill let u know.
Edited by KingCloyster (12/02/13 02:24 PM)
|
KingCloyster
Forest Mushroom 🍄



Registered: 05/27/13
Posts: 597
Loc: Quetico, Canada
Last seen: 1 year, 17 days
|
Re: Accidentally went up to 24 psi when making slants with popsicle sticks... Out of agar now. [Re: KingCloyster]
#19217523 - 12/02/13 02:23 PM (10 years, 1 month ago) |
|
|
it looks as if the same amount of agar is in the tubes... i tightened them before Pcing and i just checked one and the cap hadnt loosened. There was alot of condensation in the tubes tho, should i slosh them around a bit and try to get that off the walls of the slant tube?
Edited by KingCloyster (12/02/13 02:37 PM)
|
|
|
You cannot start new topics / You cannot reply to topics HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a 736 topic views. 44 members, 236 guests and 33 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ] |
|