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North Spore Shop: Injection Grain Bag, North Spore Mushroom Grow Kits & Cultivation Supplies

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OfflineKingCloyster
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Registered: 05/27/13
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Accidentally went up to 24 psi when making slants with popsicle sticks... Out of agar now.
    #19217274 - 12/02/13 01:24 PM (10 years, 1 month ago)

I used the last of my LME on 6 slant tubes with water-soaked popsicle sticks in them.

But I was busy with an agar>grain transfer that wasnt going my way [Fuckin agar wouldnt drop off my scalpel when trying to transfer X(] & ended up completely spacing the Sterilizer was still going and it went up to 23-24 psi for like 5 minutes. 

Am I screwed? I cant re do it cuz im out of agar. but I fear the agar may have carmelized or i might have changed the properties of the wood stick.

Its cooling right now.


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Edited by KingCloyster (12/02/13 01:25 PM)


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OfflineeLeSDenes
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Re: Accidentally went up to 24 psi when making slants with popsicle sticks... Out of agar now. [Re: KingCloyster]
    #19217299 - 12/02/13 01:32 PM (10 years, 1 month ago)

So what do you want to hear?you'll probably find it out yourself in about 3 hours. Agar supplies are chep. Shit happens. Don't panic


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OfflineKingCloyster
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Re: Accidentally went up to 24 psi when making slants with popsicle sticks... Out of agar now. [Re: eLeSDenes]
    #19217309 - 12/02/13 01:35 PM (10 years, 1 month ago)

They are hard to find in my area... I just want to know if going up to 24 psi for a bit would ruin a slant with LMEA and a popsicle stick


I wouldnt want to lose any isolates


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OfflineRogerRabbitM
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Re: Accidentally went up to 24 psi when making slants with popsicle sticks... Out of agar now. [Re: KingCloyster]
    #19217318 - 12/02/13 01:38 PM (10 years, 1 month ago)

24 psi didn't caramelize the agar.  I would be surprised if it didn't boil over though.  If the slant and agar is intact, it's OK.
RR


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"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
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OfflineKingCloyster
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Registered: 05/27/13
Posts: 597
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Re: Accidentally went up to 24 psi when making slants with popsicle sticks... Out of agar now. [Re: RogerRabbit]
    #19217343 - 12/02/13 01:43 PM (10 years, 1 month ago)

We'll see in about 20 minutes and ill let u know.


Edited by KingCloyster (12/02/13 02:24 PM)


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OfflineKingCloyster
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Registered: 05/27/13
Posts: 597
Loc: Quetico, Canada
Last seen: 1 year, 17 days
Re: Accidentally went up to 24 psi when making slants with popsicle sticks... Out of agar now. [Re: KingCloyster]
    #19217523 - 12/02/13 02:23 PM (10 years, 1 month ago)

it looks as if the same amount of agar is in the tubes... i tightened them before Pcing and i just checked one and the cap hadnt loosened. There was alot of condensation in the tubes tho, should i slosh them around a bit and try to get that off the walls of the slant tube?


Edited by KingCloyster (12/02/13 02:37 PM)


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North Spore Shop: Injection Grain Bag, North Spore Mushroom Grow Kits & Cultivation Supplies


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