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Gosho
Stranger

Registered: 12/02/13
Posts: 6
Last seen: 9 years, 10 months
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No signs of mycelium growth
#19216058 - 12/02/13 06:33 AM (10 years, 2 months ago) |
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I got 2 syringes from spores 101 (golden teacher,did everything by the book very sterile,waited long enough for the jars to cool down and almost 2 weeks later of incubation at a temperature of 80-85(in my cupboard whit a light bulb on the lower shelf to keep warm) and no mycelium.My guess was syringe was bad so I tried again and used the other one,put a very generous amount of spores in each of the 4 jars 4 days ago.This is my first time and either I`m doing something wrong ore the spores are bad .btw I used vermiculite and BRF( I couldn't find BRF so I grinded up some brown rice)and the syringes where traveling 15 days from canada to bulgaria.
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DirtyTomFlint
( ಥـْـِـِـِـْಥ)




Registered: 11/26/13
Posts: 1,879
Loc: United Kingdom
Last seen: 1 year, 2 months
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Re: No signs of mycelium growth [Re: Gosho]
#19216065 - 12/02/13 06:35 AM (10 years, 2 months ago) |
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See how the other syringe goes. Sounds to me like it's bad spores.
--------------------
   Know Your Body, Know Your Mind, Know Your Substance, Know Your Source
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PussyFart
Retired Cultivation Extrodinaire



Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
Last seen: 1 month, 11 days
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Re: No signs of mycelium growth [Re: Gosho]
#19216069 - 12/02/13 06:38 AM (10 years, 2 months ago) |
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Quote:
Gosho said: I got 2 syringes from spores 101
Welcome to The Shroomery!
I hope they did not screw you....they are notorious for screwing our members....that website is just straight up bogus.
Quote:
Gosho said: I got 2 syringes from spores 101 (golden teacher,did everything by the book very sterile,waited long enough for the jars to cool down and almost 2 weeks later of incubation at a temperature of 80-85(in my cupboard whit a light bulb on the lower shelf to keep warm) and no mycelium.My guess was syringe was bad so I tried again and used the other one,put a very generous amount of spores in each of the 4 jars 4 days ago.This is my first time and either I`m doing something wrong ore the spores are bad .btw I used vermiculite and BRF( I couldn't find BRF so I grinded up some brown rice)and the syringes where traveling 15 days from canada to bulgaria.
I honestly think you got screwed.....
Buy spores from one of our sponsors and try again.
I would also suggest following this tek for your next run....
Here is some much needed, updated info for ya.......
Light, Temperature, and Pinning Triggers...
Jars/bags/tubs/trays should colonize @ room temperature getting ambient/indirect light.
Main pinning triggers are full colonization, FAE and Evaporation off of the substrate.
Light is a secondary pinning trigger. For tropical species temperature is not a pinning factor.
P. Cubensis are a tropical species. You could colonize at 70F and fruit at 80F with great results.
Light has been proven beneficial during all stages of mycellium growth. Mushrooms like mammals have a circadian rhythm.
You want ambient/indirect light(on a 12/12 schedule preferably) for colonization and consolidation.
You want direct/intense 6500K light on a 12/12 schedule for fruiting.
Optimal temps are mid 70s throughout the whole grow, but anywhere from 65F-80F is acceptable.
Incubation is outdated/uneeded unless temps in the range stated above cannot be kept.
The inside of the jar is always a few degrees warmer than the outside because the mycellium produces heat..mycellium tends to stall at temps above 86F , and contams thrive.
Fruiting at cooler temps tends to produce denser, meatier fruits, while fruiting at higher temps will often produce hollow, less dense stems.
Mycelium should be exposed to ambient room light from day of inoculation as has been known for many years. Light is not a pinning trigger until after full colonization and an increase in air is given, and even then it's a secondary pinning trigger. RR
--------------------
THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT! A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD, SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List, EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE *** *** AFGHAN KUSH GROW LOG *** ***
Edited by PussyFart (12/02/13 06:39 AM)
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Stromrider
This must be the place



Registered: 06/02/13
Posts: 7,338
Loc: Dept of know what I'm say...
Last seen: 7 hours, 44 minutes
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Re: No signs of mycelium growth [Re: PussyFart]
#19216151 - 12/02/13 07:12 AM (10 years, 2 months ago) |
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Exactly
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DetectiveLefty
Nullius in verba


Registered: 10/12/13
Posts: 177
Loc: Floriduh, USA
Last seen: 3 years, 11 months
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Re: No signs of mycelium growth [Re: Stromrider]
#19216193 - 12/02/13 07:30 AM (10 years, 2 months ago) |
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Could be a bad syringe; Out of 3 syringes from Spores 101, 1 was bad (no germination at all), the other 2 were totally fine. All the jars were inoculated & colonizing in the same conditions. Doesn't make [economic] sense that they'd do this intentionally- I'm guessing the bad one was either old or mislabeled. More likely incompetence rather than malice.
-------------------- I swear on a stack of Bibles that everything I said in the above post is the 100% literal Truth.
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PussyFart
Retired Cultivation Extrodinaire



Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
Last seen: 1 month, 11 days
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Quote:
DetectiveLefty said: Doesn't make [economic] sense that they'd do this intentionally- I'm guessing the bad one was either old or mislabeled. More likely incompetence rather than malice.
Makes perfect economical sense actually.....they have a very catchy website and sell bogus syringes to noobs who don't know any better and chalk it up as a loss on their end.
They make money hand over fist by fucking people......they have for years.....the only thing we can do is alert peeps like this.
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THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT! A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD, SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List, EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE *** *** AFGHAN KUSH GROW LOG *** ***
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dessidoll93
Stranger
Registered: 11/15/13
Posts: 13
Last seen: 6 years, 10 months
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Re: No signs of mycelium growth [Re: PussyFart]
#19216614 - 12/02/13 10:31 AM (10 years, 2 months ago) |
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Yeeeeea,you probably got screwed. I got two syringes of dud spores from them. Same thing happened to me, followed everything and no mycelium growth. If I look at the syringes they sent me, versus any other ones I've received. You can clearly see that there are barely, and I mean BARELY, any spores in theirs. Long story short, I will NEVER order from them again.
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DrugsRGood
Analytical Anarchist



Registered: 07/10/09
Posts: 689
Loc: Earth
Last seen: 7 years, 7 months
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Re: No signs of mycelium growth [Re: dessidoll93]
#19216664 - 12/02/13 10:48 AM (10 years, 2 months ago) |
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Yea Spores 101 should be burned at the stake.
-------------------- Life is an adventure; not knowing what's ahead brings a great sense of anticipation; and meeting women is the reward.
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BittrBuffalo
Deaconica

Registered: 05/19/13
Posts: 1,729
Loc: Church of the SubGenus
Last seen: 3 years, 5 months
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Re: No signs of mycelium growth [Re: PussyFart]
#19216699 - 12/02/13 10:57 AM (10 years, 2 months ago) |
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Quote:
Notahacker420 said: I honestly think you got screwed.....
Buy spores from one of our sponsors and try again.
I would also suggest following this tek for your next run....
Here is some much needed, updated info for ya.......
Light, Temperature, and Pinning Triggers...
Jars/bags/tubs/trays should colonize @ room temperature getting ambient/indirect light.
Main pinning triggers are full colonization, FAE and Evaporation off of the substrate.
Light is a secondary pinning trigger. For tropical species temperature is not a pinning factor.
P. Cubensis are a tropical species. You could colonize at 70F and fruit at 80F with great results.
Light has been proven beneficial during all stages of mycellium growth. Mushrooms like mammals have a circadian rhythm.
You want ambient/indirect light(on a 12/12 schedule preferably) for colonization and consolidation.
You want direct/intense 6500K light on a 12/12 schedule for fruiting.
Optimal temps are mid 70s throughout the whole grow, but anywhere from 65F-80F is acceptable.
Incubation is outdated/uneeded unless temps in the range stated above cannot be kept.
The inside of the jar is always a few degrees warmer than the outside because the mycellium produces heat..mycellium tends to stall at temps above 86F , and contams thrive.
Fruiting at cooler temps tends to produce denser, meatier fruits, while fruiting at higher temps will often produce hollow, less dense stems.
Mycelium should be exposed to ambient room light from day of inoculation as has been known for many years. Light is not a pinning trigger until after full colonization and an increase in air is given, and even then it's a secondary pinning trigger. RR
All of these things! Good luck.
-------------------- Disclaimer: This post is a work of fiction, provided for entertainment purposes only. Any resemblance to actual persons or events, past or present, is strictly coincidental. All celebrity voices are impersonated. If you begin your ID request with, "I just ate a bunch of these mushrooms…should I not have done that?" I'm just gonna sit back and watch Darwin at work.
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