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Offlinedessidoll93
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Registered: 11/15/13
Posts: 13
Last seen: 6 years, 10 months
Black mold? or some other kind of contam? (pictures included)
    #19216595 - 12/02/13 10:26 AM (10 years, 2 months ago)

Hey there! I'm a pretty new member although I've been lurking for years! Sad this has to be my first post but....anywho
This is my first legitimate grow (I've tried once before but I had dud spores that never even began to colonize). It's been a week and two days since inoculation. I went home for break on days 5-7 and came back to mycelium growth (of which there was none when I left)! Now, two days later, I checked my jars and 3 of them have a black area in them.
I'm not quite sure how to describe it. I didn't think I saw any signs of contam when I checked them the first time but looking back at the video I recorded, I think I may have just been too excited to notice (because I do see some specks in the video). Anyways, the black area is definitely getting larger (growing?) but so is the mycelium...
I've isolated the jars from the others but I haven't thrown them out yet because I'm hoping it's something salvagable. The spots don't look like any other black contam I've seen online. There are no "wetspots" or fuzz or smell. Maybe it's another kind of contam? Or maybe (hopefully) it'll go away? I've read on some posts that cultivators have had black areas in their jars that went away and their cakes fruited fine. I've included pictures. Thanks for the help (and sorry for all of the parenthesis)!

















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Offlinemongolian
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Registered: 10/04/13
Posts: 48
Last seen: 10 years, 1 month
Re: Black mold? or some other kind of contam? (pictures included) [Re: dessidoll93]
    #19216640 - 12/02/13 10:39 AM (10 years, 2 months ago)

Hi,
I'm a new grower too.
These black areas are really big... I mean you can keep them growing to see if the mycelium "eats it", but I guess it will overtake yours in some days.
If it does not go away DON'T fruit it, black mold is known to be dangerous to your health and the spores could fuck up your future grows... If you wanna safe the jar pc for 90 mins toss outside and wash the jar again.

(PS:you can see my contam worries here: http://www.shroomery.org/forums/showflat.php/Number/19203920):D


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InvisibleSkinty
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Registered: 07/04/13
Posts: 1,150
Re: Black mold? or some other kind of contam? (pictures included) [Re: mongolian]
    #19216802 - 12/02/13 11:27 AM (10 years, 2 months ago)

Could be Aspergillus niger...I would PC the jars before opening and throw that crap out after :sad:


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InvisibleJohnnieYen
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Registered: 03/15/11
Posts: 3,529
Loc: City Z
Re: Black mold? or some other kind of contam? (pictures included) [Re: dessidoll93]
    #19216805 - 12/02/13 11:28 AM (10 years, 2 months ago)

Toss them ASAP. you will get black mold spores in the air once you shake those out.

Best to PC them for an hour at 15psi to kill everything. Just to be safe


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OfflineImFukNCLUELESS
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Registered: 11/19/12
Posts: 1,580
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Re: Black mold? or some other kind of contam? (pictures included) [Re: JohnnieYen]
    #19217058 - 12/02/13 12:29 PM (10 years, 2 months ago)

Throw it away asap


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Offlinedessidoll93
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Registered: 11/15/13
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Last seen: 6 years, 10 months
Re: Black mold? or some other kind of contam? (pictures included) [Re: ImFukNCLUELESS]
    #19217302 - 12/02/13 01:33 PM (10 years, 2 months ago)

Thanks guys! Well I guess the verdict is in? But should I wait or toss them now? Also, I don't have a pressure cooker, I sterilized in a large pot(for 2hours). Can I put them in there again and throw out the substrate or just throw out the whole jar?


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Offline2bittoker
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Registered: 03/09/13
Posts: 555
Last seen: 2 years, 4 months
Re: Black mold? or some other kind of contam? (pictures included) [Re: dessidoll93]
    #19217357 - 12/02/13 01:46 PM (10 years, 2 months ago)

Quote:

dessidoll93 said:
Thanks guys! Well I guess the verdict is in? But should I wait or toss them now? Also, I don't have a pressure cooker, I sterilized in a large pot(for 2hours). Can I put them in there again and throw out the substrate or just throw out the whole jar?



Since your no longer worried about ruining the moisture content of your substrate, you can just boil them and not worry about aluminum foil or elevating them out of the water.  Definitely sanitize those jars.  Black mold is nothing to play with. 

Boil for 90 minutes at a rolling boil, then empty outside directly into a plastic bag, and tie completely shut, and dispose of.  Boil jars again, afterwards.


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“I slept and dreamt that life was joy. I awoke and saw that life was service. I acted and behold, service was joy.”
"Love does not claim possession, but gives freedom"
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Offlinesof4r0ckeRs1984
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Registered: 07/30/10
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Re: Black mold? or some other kind of contam? (pictures included) [Re: 2bittoker]
    #19217391 - 12/02/13 01:51 PM (10 years, 2 months ago)

Let me guess: You're a first timer and you bought some readymade syringes from a vendor?

I'm pretty sure this is just some charcoal form the syringes. Some vendors put charcoal in their syringes to make the "spore solution" look really thick and black.


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Offline2bittoker
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Registered: 03/09/13
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Re: Black mold? or some other kind of contam? (pictures included) [Re: sof4r0ckeRs1984]
    #19217404 - 12/02/13 01:55 PM (10 years, 2 months ago)

Quote:

sof4r0ckeRs1984 said:
Let me guess: You're a first timer and you bought some readymade syringes from a vendor?

I'm pretty sure this is just some charcoal form the syringes. Some vendors put charcoal in their syringes to make the "spore solution" look really thick and black.



What are you talking about?  I've used almost every shroomery spore vendor and have never had black soot end up in my cakes.  Additionally OP said above black growth appeared days after inoculation.  Charcoal is not alive, it will not suddenly appear and grow bigger. 

Here is a picture of a MS vendor syringe inoculated jar I have right now.


--------------------
“I slept and dreamt that life was joy. I awoke and saw that life was service. I acted and behold, service was joy.”
"Love does not claim possession, but gives freedom"
― Rabindranath Tagore

Stuff for New Growers
Where new growers should start: RogerRabbit's PF Tek video    How it Should and Shouldn't Look 
My Simplified Bulk Growing  My OJ Shroom Tek


Edited by 2bittoker (12/02/13 01:56 PM)


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Offlinedessidoll93
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Registered: 11/15/13
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Last seen: 6 years, 10 months
Re: Black mold? or some other kind of contam? (pictures included) [Re: sof4r0ckeRs1984]
    #19217408 - 12/02/13 01:56 PM (10 years, 2 months ago)

Yes I did by ready made syringes! And they were reeeeallly black and fluffy. But would the charcoal spread? I mean don't get me wrong, I would love for it to be charcoal, but those spots didn't start out that big!


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InvisibleJohnnieYen
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Registered: 03/15/11
Posts: 3,529
Loc: City Z
Re: Black mold? or some other kind of contam? (pictures included) [Re: sof4r0ckeRs1984] * 1
    #19217410 - 12/02/13 01:56 PM (10 years, 2 months ago)

I doubt it was charcoal. I've seen that contamination before and it appears out of nowhere. One day jar looks fine, next day black spot, then it gets bigger every day.

That is 100% black mold.


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Offline2bittoker
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Registered: 03/09/13
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Re: Black mold? or some other kind of contam? (pictures included) [Re: dessidoll93]
    #19217414 - 12/02/13 01:57 PM (10 years, 2 months ago)

Quote:

dessidoll93 said:
Yes I did by ready made syringes! And they were reeeeallly black and fluffy. But would the charcoal spread? I mean don't get me wrong, I would love for it to be charcoal, but those spots didn't start out that big!



I'll repeat what I said
"
Additionally OP said above black growth appeared days after inoculation.  Charcoal is not alive, it will not suddenly appear and grow bigger. 
"


--------------------
“I slept and dreamt that life was joy. I awoke and saw that life was service. I acted and behold, service was joy.”
"Love does not claim possession, but gives freedom"
― Rabindranath Tagore

Stuff for New Growers
Where new growers should start: RogerRabbit's PF Tek video    How it Should and Shouldn't Look 
My Simplified Bulk Growing  My OJ Shroom Tek


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Invisible36fuckin5
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Registered: 08/11/03
Posts: 12,081
Loc: Diving into Mystical Territori...
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Re: Black mold? or some other kind of contam? (pictures included) [Re: 2bittoker]
    #19217630 - 12/02/13 02:51 PM (10 years, 2 months ago)

I'd boil those, then throw them away without opening them. Maybe bag them up.

Black molds are nothing to take a chance with.


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bodhisatta said:
i recommend common sense and figuring it out.

These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.


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OfflineRogerRabbitM
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Re: Black mold? or some other kind of contam? (pictures included) [Re: dessidoll93]
    #19217670 - 12/02/13 03:01 PM (10 years, 2 months ago)

Is that dark spot growing?  It doesn't look like mold.  If it's mold it will look like black powder on the surface.  I doubt mold spores could germinate, grow, and then sporulate in just a few days.

If the spot isn't growing, ignore it and see if the mycelium colonizes into it.  If the mycelium tries to form a ring around the area instead of penetrating it, toss the jar out.
RR


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InvisibleJohnnieYen
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Registered: 03/15/11
Posts: 3,529
Loc: City Z
Re: Black mold? or some other kind of contam? (pictures included) [Re: RogerRabbit]
    #19217962 - 12/02/13 04:09 PM (10 years, 2 months ago)

He said the black is growing and happened 9 days after inoculation.

I don't think OP should keep those jars any longer in my opinion.


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Offline2bittoker
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Registered: 03/09/13
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Re: Black mold? or some other kind of contam? (pictures included) [Re: JohnnieYen]
    #19217990 - 12/02/13 04:16 PM (10 years, 2 months ago)

Quote:

JohnnieYen said:
He said the black is growing and happened 9 days after inoculation.

I don't think OP should keep those jars any longer in my opinion.



Yeah you beat me to it.  OP stated above black growth appeared 9 days after sterilization and inoculation.  2 days after seeing mushroom myclieum growth. 

Also, in the last 3 pictures you can see metabolites forming where the black growth is starting to merge with mushroom mycelium.


--------------------
“I slept and dreamt that life was joy. I awoke and saw that life was service. I acted and behold, service was joy.”
"Love does not claim possession, but gives freedom"
― Rabindranath Tagore

Stuff for New Growers
Where new growers should start: RogerRabbit's PF Tek video    How it Should and Shouldn't Look 
My Simplified Bulk Growing  My OJ Shroom Tek


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Offlinedessidoll93
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Registered: 11/15/13
Posts: 13
Last seen: 6 years, 10 months
Re: Black mold? or some other kind of contam? (pictures included) [Re: 2bittoker]
    #19229872 - 12/05/13 02:23 AM (10 years, 2 months ago)

Thanks for all your help guys! And I'm a woman btw haha. I have since thrown out the bad jars but in the last 3 days TWO more of them have developed a black spot! I'm not sure what it's from! I check them very day for contams but I wear gloves and use disinfectant wipes AND rubbing alcohol on them (the gloves) before I touch the jars. I even moved them into an empty, never before used drawer (which I also disinfected). I don't know what else to do! I'm down to 5 jars now! At this rate, I'm not sure any of them will survive!


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Offlinedessidoll93
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Registered: 11/15/13
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Last seen: 6 years, 10 months
Re: Black mold? or some other kind of contam? (pictures included) [Re: dessidoll93]
    #19229883 - 12/05/13 02:35 AM (10 years, 2 months ago)

I forgot to mention that they're my fastest growing jars, they're both at about 70-80% while the others are at 40-60%.


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InvisibleJohnnieYen
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Registered: 03/15/11
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Re: Black mold? or some other kind of contam? (pictures included) [Re: dessidoll93]
    #19230733 - 12/05/13 11:02 AM (10 years, 2 months ago)

How did you sterilize? PC or steam bath and for how long.

Also, I didn't see a vermiculite layer? if you have one how thick is it?

Third would be sterile technique? did you flame sterilize your needle?
open air, or still air box?

other than that it could be a bad syringe... these things do unfortunately happen

Start another project to pass the time. Is disheartening to have to toss everything.


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Offlinedessidoll93
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Re: Black mold? or some other kind of contam? (pictures included) [Re: JohnnieYen]
    #19230901 - 12/05/13 11:59 AM (10 years, 2 months ago)

I steamed them in a large pot. I do have a 1/2 inch verm layer. I did flame my needle, as well as wiped it with alcohol. BUT I did inoculate in open air but there wasn't any air on and I disinfected as best as I could. I'm just so sad to seem them go!


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