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duck_metal
Stranger


Registered: 12/02/13
Posts: 2
Loc: Pac-NW
Last seen: 9 years, 11 months
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Experience/Advice.. Cut away 1 pint PF teck BRF Jars
#19215783 - 12/02/13 03:13 AM (10 years, 1 month ago) |
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Hello Shroomery, First time posting... been reading for awhile.
This post is intended to gather some experience and/or advice about what will happen as a result of the following "story".. and also serve as an experiment that perhaps others can learn from.
PF tek was used for 12 (one)pint BRF jars.
Things that probably should have been done differently: -Used half pint jars -Seeing as how one pint jars were used, should have used 4 inoculation points instead of 2. Also, the 2 points that were used on each jar might have been located too close to the center.. should have been closer to the rim of the jars.
Heres what happened; The 12 jars were inoculated at 2 points(holes) for each jar almost exactly a month ago. Temperature wasn't as consistent as it should have been, fluctuating between 78 and 90. Over the last two weeks the temperature was kept more consistent at between 78 and 84. The mycelium growth was slow throughout but seemed to have stalled recently. Two of the 12 jars came out nicely but the remaining 10 still had uncolonized substrate at the bottom of the jars. So it was decided that one jar (the least promising of the remaining 10) would be birthed and cased to see what would happen. Upon being birthed it was discovered that under the uncolonized substrate the mycelium had grown together nicely. The excess was gently wiped away and then rinsed off. Imagine a normal healthy cake in the shape of the jar.. missing a "V" section at the bottom.
Question 1. Is this normal? If any given cake is birthed early would the result be the same.. would it appear to be colonized through the substrate up to the point where there remained visible uncolonized substrate?
Since 2 jars seem likely to yield fruit and the least promising "experiment" jar looked like it was in otherwise good condition it was decided that another should be birthed to see what it looked like. The next was the same as described above.. an otherwise healthy colony missing a "V" section once the excess substrate was wiped/rinsed away. Its as if the 10 stalled jars simply couldn't colonize the remaining substrate at the bottom.
Question 2. How can this happen? No foul smell or discoloration was detected. Surprisingly, all of the 12 jars appears free of any obvious contamination. Could the substrate at the bottom have become too tightly packed to be colonized?
Question 3. Given the provided information/observations.. were these cakes simply birthed early?
So to the experiment part; All 10 of the jars that contained uncolonized substrate were birthed, wiped and rinsed. They are currently soaking in a pot (dunk and roll). Should it be expected that these cakes become contaminated with mold? Logic suggest that after being inspected/rolled they should be kept in a separate fruiting chamber just in case (provided they still appear viable). What do you think will happen? Anyone have knowledge/experience having done this?
I intend on posting updates as to how the rest of this "story" turns out.
As an aside... I don't mean to be a d'bag writing like this.. but I read the posting guide lines and it seems like writing this way doesn't incriminate anyone for doing anything illegal.. <snicker snicker>.
Thanks for reading
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Stromrider
This must be the place



Registered: 06/02/13
Posts: 7,326
Loc: Dept of know what I'm say...
Last seen: 1 hour, 12 minutes
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Re: Experience/Advice.. Cut away 1 pint PF teck BRF Jars [Re: duck_metal]
#19215792 - 12/02/13 03:20 AM (10 years, 1 month ago) |
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Yes using pint jars was your problem. The substrate at the bottom gets too tightly compacted and can't colonize. Use the short fat wide mouth half pint jars for best results
Birthing cakes that aren't fully colonized always ends up contaminated before it fruits.
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monoculture
Mycaddict



Registered: 10/16/13
Posts: 246
Last seen: 9 years, 5 months
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Re: Experience/Advice.. Cut away 1 pint PF teck BRF Jars [Re: Stromrider]
#19215811 - 12/02/13 03:36 AM (10 years, 1 month ago) |
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Quote:
Birthing cakes that aren't fully colonized always ends up contaminated before it fruits.
Also when the uncolonized substrate is washed away? When he would leave it on there I would say sure it will contaminate, but now he only has mycelium, which should be resistant? Or is there another reason it would contaminate?
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duck_metal
Stranger


Registered: 12/02/13
Posts: 2
Loc: Pac-NW
Last seen: 9 years, 11 months
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Re: Experience/Advice.. Cut away 1 pint PF teck BRF Jars [Re: monoculture]
#19215827 - 12/02/13 03:49 AM (10 years, 1 month ago) |
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Quote:
monoculture said:
Quote:
Birthing cakes that aren't fully colonized always ends up contaminated before it fruits.
Also when the uncolonized substrate is washed away? When he would leave it on there I would say sure it will contaminate, but now he only has mycelium, which should be resistant? Or is there another reason it would contaminate?
This was the idea... Also hoping that the casing layer might provide some small measure of defense against contaminates if the mycelium simply hadn't consolidated enough under the uncolonized "V" sections.
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monoculture
Mycaddict



Registered: 10/16/13
Posts: 246
Last seen: 9 years, 5 months
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Re: Experience/Advice.. Cut away 1 pint PF teck BRF Jars [Re: duck_metal]
#19215853 - 12/02/13 04:04 AM (10 years, 1 month ago) |
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Keep us updated whatever happens. In my experience the incubation temp also matters a lot. When it is too hot, cakes will stall and contams will thrive. I have a incubator myself, though I know it is said 100's of times they are no good. But because I have my shrooms growing in a confined space, also to keep them out of sight, I put a heater in there with a thermostat to keep the temp more stable between the day/night. I turned the heat up at a certain moment there were only a few jars in there (caused by that cubensis temp-graph that is here at multiple posts that is 100% wrong info), and got stalled jars. Now I keep it at 21 to max 24 degrees C and everything goes fine. If the heater would not be there, they would be 17 deg C in the night. I can imagine a bigger jar would also heat up more, so incubating that at an even lower temp would be even better. Being a bit cold, mycelium might colonize a bit slower, being too hot, mycelium will almost stop colonizing and bacteria/yeast will thrive.
Edited by monoculture (12/02/13 11:02 AM)
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