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Invisiblechimpezman
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Registered: 03/21/13
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[UPDATE]: Agar - Cobweb or Mycelium (w/ pics)
    #19096445 - 11/06/13 05:07 PM (10 years, 3 months ago)

Title says it all. The growths I see on my petri dishes seems a bit sketchy to me. They all seemed WAY too wispy to me. I have mild experience growing, but just started into Agar.
I will note that picture 1 is an awful pic and I can upload another if necessary. What do you guys think?


UPDATE:

I have made further progress. I tried my best to navigate through what appears to be cobweb. Unless i just have some really fluffy mycelium that is...
1-4 seem to be my best outlook. The rest seem too fluffy and possibly infected with cobweb.
Are any of these worth saving? 




Edited by chimpezman (11/30/13 11:46 PM)


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OfflineStromriderM
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Re: Agar - Cobweb or Mycelium (w/ pics) [Re: chimpezman]
    #19096665 - 11/06/13 05:45 PM (10 years, 3 months ago)

Definitely got some contams going on. Well I think. Damn those pics suck


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OfflineSockadin
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Re: Agar - Cobweb or Mycelium (w/ pics) [Re: Stromrider]
    #19096735 - 11/06/13 05:57 PM (10 years, 3 months ago)

Yeah I would say they all are contaminated. I think the first dish has mycelium in it. But it also looks like bacteria in all three.
I would try again, nothing there that looks worth transferring and cleaning up imo.


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Invisiblechimpezman
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Re: Agar - Cobweb or Mycelium (w/ pics) [Re: Sockadin]
    #19096878 - 11/06/13 06:25 PM (10 years, 3 months ago)

mehhhhhhhhh :frown:


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Invisiblepoopy mcpooperson
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Re: Agar - Cobweb or Mycelium (w/ pics) [Re: chimpezman]
    #19096917 - 11/06/13 06:33 PM (10 years, 3 months ago)

did you use MS syringe solution? it looks like it from the wet spots on the agar around the myc growth. i definitely see a few areas that you can transfer from. i am new to agar and have been having wonderful success.

Dish 1- i dont know what is going on in there. it looks like cobweb from other photos that ive seen but i cant personally advise on anything from my experience.
Dish 2- 7-8 o'clock
Dish 3- the growth that is on the far left. i would take a triangle out of the 11 o'clock position
Dish 4- 5 o'clock


--------------------
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Invisiblechimpezman
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Re: Agar - Cobweb or Mycelium (w/ pics) [Re: poopy mcpooperson]
    #19103107 - 11/07/13 09:55 PM (10 years, 3 months ago)

Ended up using spore prints on inoculation loop, that's it. I used a MS syringe for one, but it seemed to get the most contaminations. I am also not very careful when taking prints. I just sort of willy nilly take them and hope for the best :goodluckwiththat2: lol

I had 3 dishes left over that were clean, I'll transfer a few and see what happens. Worst comes to worst, I waste a dish or two. Rather practice my technique and keep working at it in the long run anyway. I appreciate your input! Thanks.


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Invisiblechimpezman
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Re: Agar - Cobweb or Mycelium (w/ pics) [Re: chimpezman]
    #19210778 - 12/01/13 12:17 AM (10 years, 2 months ago)

I have updates...and questions again! Any help appreciated as always.


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InvisiblePastywhyteMDiscord
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Re: Agar - Cobweb or Mycelium (w/ pics) [Re: chimpezman]
    #19210803 - 12/01/13 12:27 AM (10 years, 2 months ago)

IMO I'd make a transfer from 4 o'clock on plate one and from 3 o'clock and 6 o'clock on plate 4. I'd also give 11 o'clock and 1 o'clock a try on the last plate and maybe if you got plates to play with a transfer from 3 and 4 on plate 2.


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Invisiblechimpezman
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Re: Agar - Cobweb or Mycelium (w/ pics) [Re: Pastywhyte]
    #19210830 - 12/01/13 12:37 AM (10 years, 2 months ago)

Thanks! I've got at least 4 dishes left over. I will post results and let you know. I'm worried the cobweb if there is any will follow me to the grave  :dislike:


Edited by chimpezman (12/01/13 12:37 AM)


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InvisiblePastywhyteMDiscord
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Re: Agar - Cobweb or Mycelium (w/ pics) [Re: chimpezman]
    #19210845 - 12/01/13 12:39 AM (10 years, 2 months ago)

Cobweb moves pretty fast and IME is very aerial. If it had made it onto these plates you would know by now.


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InvisibleFrankHorrigan
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Re: [UPDATE]: Agar - Cobweb or Mycelium (w/ pics) [Re: chimpezman]
    #19211088 - 12/01/13 02:19 AM (10 years, 2 months ago)

Quote:

chimpezman said:
Title says it all. The growths I see on my petri dishes seems a bit sketchy to me. They all seemed WAY too wispy to me. I have mild experience growing, but just started into Agar.
I will note that picture 1 is an awful pic and I can upload another if necessary. What do you guys think?


UPDATE:

I have made further progress. I tried my best to navigate through what appears to be cobweb. Unless i just have some really fluffy mycelium that is...
1-4 seem to be my best outlook. The rest seem too fluffy and possibly infected with cobweb.
Are any of these worth saving? 







I think those plates look great, minus the condensation...


--------------------

Yes, you can bump my old threads with a question.
Here is how I get things done.
You should take a look. :hehehe:


Frank's tips and tricks. Updated on 3/21/14
AMU- Get an answer here -AMU


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OfflineSockadin
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Re: [UPDATE]: Agar - Cobweb or Mycelium (w/ pics) [Re: FrankHorrigan]
    #19211244 - 12/01/13 05:05 AM (10 years, 2 months ago)

The difference between a cell phone and a desktop.... Frank is correct, when you blow up the pictures they look fine. When you look at them on my crappy phone the little bit of condensation looks like bacterial blotch's. Sorry about that man, looking good.


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Invisibledusttodust


Registered: 11/26/12
Posts: 491
Re: [UPDATE]: Agar - Cobweb or Mycelium (w/ pics) [Re: FrankHorrigan]
    #19211280 - 12/01/13 05:42 AM (10 years, 2 months ago)

Quote:

FrankHorrigan said:[/
I think those plates look great, minus the condensation...



:whathesaid:
And it would be good to mention what species you are culturing when making op. Every species look diffrent.


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Invisiblechimpezman
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Re: [UPDATE]: Agar - Cobweb or Mycelium (w/ pics) [Re: dusttodust]
    #19212696 - 12/01/13 01:18 PM (10 years, 2 months ago)

Thank you everyone! I will just have to see in a few days how things go.
And the strain is Golden Teacher - species: Psilocybe cubensis.

How exactly do you avoid condensation? I have read of the coffee mug tek, but my house must fluctuate in temperature that much, condensation just appeared after about a day.


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InvisibleFrankHorrigan
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Re: [UPDATE]: Agar - Cobweb or Mycelium (w/ pics) [Re: chimpezman]
    #19212739 - 12/01/13 01:30 PM (10 years, 2 months ago)

Condensation like you have there is probably due to pouring the agar while it is still too hot. I wait about 5 hours (after sterilization) before I open my PC and pour my plates.

For keeping petris, store them in an insulated lunchbox with each other. This will help insulate against temp swings that will cause condensation to build up over time.


--------------------

Yes, you can bump my old threads with a question.
Here is how I get things done.
You should take a look. :hehehe:


Frank's tips and tricks. Updated on 3/21/14
AMU- Get an answer here -AMU


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Invisiblechimpezman
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Registered: 03/21/13
Posts: 40
Loc: USA Flag
Re: [UPDATE]: Agar - Cobweb or Mycelium (w/ pics) [Re: FrankHorrigan]
    #19212770 - 12/01/13 01:39 PM (10 years, 2 months ago)

Quote:

FrankHorrigan said:
Condensation like you have there is probably due to pouring the agar while it is still too hot. I wait about 5 hours (after sterilization) before I open my PC and pour my plates.

For keeping petris, store them in an insulated lunchbox with each other. This will help insulate against temp swings that will cause condensation to build up over time.




Thanks Frank! :thumbup:


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Invisiblechimpezman
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Re: [UPDATE]: Agar [Re: chimpezman]
    #19351190 - 12/31/13 01:31 PM (10 years, 1 month ago)

Thank you gentlemen for all of your help earlier. I have done Agar->Grain transfers from several of the previous suggestions and they are in the process of colonization.

Just one last question for any of you people experienced with agar. Are the small white dots places where mycelium cross? What are these?



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Offlinebluecap
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Re: [UPDATE]: Agar [Re: chimpezman]
    #19351200 - 12/31/13 01:35 PM (10 years, 1 month ago)

nice, that's hyphal knotting, or premordia, then when the pinheads form, pins...put that baby intosome grain, then a substrate to bulk 1/1...:smile:


--------------------
I wish I was a grain of sand, layin in a babies hand...fallin like a diamond chain into the ocean... A willow tree is strong enough to bend, never like the oak who lives in fear of a wind....Gamma...:mushroom2:                                                                                                                                                  :sporedrop:          :sporedrop:


Edited by bluecap (12/31/13 01:36 PM)


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OfflinecronicrFacebook
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Re: [UPDATE]: Agar [Re: bluecap]
    #19351211 - 12/31/13 01:37 PM (10 years, 1 month ago)

:eek:


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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