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Offlinejigglysphinxly
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Grow Log: P.Cubensis B+. WBS-> Coir (Pics Attached!) Original Topic: Tyvek substitues
    #19212717 - 12/01/13 01:25 PM (10 years, 1 month ago)

I am based in Asia. I am simple unable to source tyvek online/offline. Every grain spawn tek I have come across, uses tyvek.
Can someone recommend me a substitute? Will polyfill work?
I read the board multiple times, but each tyvek substitute thread eventually ended up in a separate debate, which didn't answer the original question.

Thanks for the help :smile:


Edited by jigglysphinxly (03/13/14 11:26 AM)


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OfflinePsilicon
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Re: Tyvek substitutes for those based outside the United States [Re: jigglysphinxly]
    #19212760 - 12/01/13 01:37 PM (10 years, 1 month ago)

That really depends on what you intend to use it for.  Would you please elaborate?


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Offlinejigglysphinxly
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Re: Tyvek substitutes for those based outside the United States [Re: Psilicon]
    #19212870 - 12/01/13 01:57 PM (10 years, 1 month ago)

Sorry, I forgot the purpose.

I was planning to do a grain spawn (millets in quart jars with p.cubensis spores)


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InvisibleFrankHorrigan
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Re: Tyvek substitutes for those based outside the United States [Re: jigglysphinxly]
    #19212889 - 12/01/13 02:01 PM (10 years, 1 month ago)

Polyfill works fine for grain jars :thumbup:


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You should take a look. :hehehe:


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OfflinePsilicon
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Re: Tyvek substitutes for those based outside the United States [Re: jigglysphinxly]
    #19212892 - 12/01/13 02:02 PM (10 years, 1 month ago)

Yes, you can successfully use polyfill to provide sterile GE for a grain jar.  :smile:


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Offlinejigglysphinxly
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Re: Tyvek substitutes for those based outside the United States [Re: Psilicon]
    #19212899 - 12/01/13 02:03 PM (10 years, 1 month ago)

Thanks :smile:


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Offlinejigglysphinxly
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Re: Tyvek substitutes for those based outside the United States [Re: jigglysphinxly]
    #19420663 - 01/15/14 12:20 AM (10 years, 16 days ago)

I used pillow polyfill, and the coir colonized completely. I just dialed in my tub. Pics attached. I will attach more pics soon. (can those white spots be hyphal knots?)


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OfflinecronicrFacebook
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Re: Tyvek substitutes for those based outside the United States [Re: jigglysphinxly]
    #19420678 - 01/15/14 12:22 AM (10 years, 16 days ago)

theres no pic:crazy:


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Offlinejigglysphinxly
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Re: Tyvek substitutes for those based outside the United States [Re: cronicr]
    #19420851 - 01/15/14 12:57 AM (10 years, 16 days ago)

Damn! I can see it though.
Here is the direct link : colonized coir


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InvisiblePestile

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Re: Tyvek substitutes for those based outside the United States [Re: jigglysphinxly]
    #19421171 - 01/15/14 03:43 AM (10 years, 16 days ago)

Good stuff! :thumbup:


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OfflineAEL1911
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Re: Tyvek substitutes for those based outside the United States [Re: Pestile]
    #19421281 - 01/15/14 04:56 AM (10 years, 16 days ago)

What part of Asia?  I'm in Japan and I can get Tyvek.


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Offlinejigglysphinxly
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Re: Tyvek substitutes for those based outside the United States [Re: AEL1911]
    #19421410 - 01/15/14 05:58 AM (10 years, 16 days ago)

More like, South East Asia - Sri Lanka :smile:


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OfflineAEL1911
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Re: Tyvek substitutes for those based outside the United States [Re: jigglysphinxly]
    #19421415 - 01/15/14 06:02 AM (10 years, 16 days ago)

Well then I have the slightest idea, sorry.  Good luck with your grow!


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Offlinejigglysphinxly
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Re: Tyvek substitutes for those based outside the United States [Re: AEL1911]
    #19421434 - 01/15/14 06:11 AM (10 years, 16 days ago)

Well, polyfill seems to have done the job. I just dialed in my monotub yesterday. I am seeing white dots all over - but not sure if these will pin or whatever. I guess patience is what I need to employ here - something I sorely lack :|


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OfflinePsytanium
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Re: Tyvek substitutes for those based outside the United States [Re: jigglysphinxly]
    #19421541 - 01/15/14 06:49 AM (10 years, 16 days ago)

Same here, we dont have tyvek, where did u use polyfill successfully? U mean covering the lid hole?


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Offlinejigglysphinxly
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Re: Tyvek substitutes for those based outside the United States [Re: Psytanium]
    #19421596 - 01/15/14 07:12 AM (10 years, 16 days ago)

Yep.
I made 5 holes in total:
(1) I made a 0.75cm diameter hole right at the centre,
(2) I made 4 needle-sized holes symmetrically around it (~1.5 cm away from the center hole, and ~1cm from the rim)
(3) I closed the 4 needle sized holes with micropore tek.
(4) For the center hole - that's where the polyfill came in. I took some polyfill, rolled it up and shoved it through the hole. As long as the hole is really "tightly" fitted in by the polyfill, it should work.
I covered the lid with a double aluminium foil layer during PC sterilization.

I inoculated through the 4 holes, and covered them again with micropore tek.


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Offlinejigglysphinxly
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Re: Tyvek substitutes for those based outside the United States [Re: jigglysphinxly]
    #19421601 - 01/15/14 07:14 AM (10 years, 16 days ago)

The polyfill should look like this: http://www.fungifun.org/English/Polyfil
The hole configuration is like this: http://www.shroomery.org/forums/showflat.php/Number/10888743


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OfflinePsilicon
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Re: Tyvek substitutes for those based outside the United States [Re: jigglysphinxly]
    #19424274 - 01/15/14 06:48 PM (10 years, 15 days ago)

Quote:

jigglysphinxly said:
Well, polyfill seems to have done the job. I just dialed in my monotub yesterday. I am seeing white dots all over - but not sure if these will pin or whatever. I guess patience is what I need to employ here - something I sorely lack :|




Nope, those won't pin.  It's just a little bit of aerial mycelium.

Not to worry, though.  That looks like a very healthy tub, and I'd expect to see primordia at some point between 4-13 days from now.  When you see them, they'll be much whiter, much smaller and unmistakeable.  You won't have to ask.  I think you'll find Tmethyl's thread on how it should and shouldn't look to be very helpful in the meantime.


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Offlinejigglysphinxly
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Re: Tyvek substitutes for those based outside the United States [Re: Psilicon]
    #19441806 - 01/19/14 09:57 AM (10 years, 12 days ago)

So I have tons of pins. I will post pics soon. Two quick questions:
(1) I had more pins on the sides so I tightened the polyfill a little - please let me know if this is a good strategy
(2) A few side pins are fully grown almost - should I harvest these (the cap has begun to expand)

I also want to take a print and clone. This has been such a great success :laugh:

Will post pics tomorrow.


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OfflinePsilicon
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Re: Tyvek substitutes for those based outside the United States [Re: jigglysphinxly]
    #19445090 - 01/19/14 10:37 PM (10 years, 11 days ago)

Quote:

jigglysphinxly said:
So I have tons of pins. I will post pics soon. Two quick questions:
(1) I had more pins on the sides so I tightened the polyfill a little - please let me know if this is a good strategy



Nope.  That will reduce pinset across the board, not just on the sides, and it'll lead to deformities like fuzzy feet and spaghetti-stalk mushrooms.  You don't want that.


Quote:

jigglysphinxly said:(2) A few side pins are fully grown almost - should I harvest these (the cap has begun to expand)

I also want to take a print and clone. This has been such a great success :laugh:

Will post pics tomorrow.




Harvest them when it looks like the veil is about to tear, or when you start seeing a few spores on the stipe of the mushroom.  Just before they do so the color will change from brown to tan, and the shape of the cap will change.


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Offlinejigglysphinxly
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Re: Tyvek substitutes for those based outside the United States [Re: Psilicon]
    #19445817 - 01/20/14 02:10 AM (10 years, 11 days ago)

Thanks for the info.
I went ahead and tightened the polyfill (before reading your post), and I *feel* the humidity has definitely improved. My polyfill was really loose (I saw very little condensation on the tub). I see a lot of condensation now, with circles of evaporation around the polyfilled holes. I am going to keep it like this for a day and observe if it improves the pin set.
One last question - harvesting the side pins: I am planning to do the flipping tek - where you put one monotub in another, flip it over, harvest, and put it back. Can this damage the pin sets on top? (since when the cake is flipped over, the weight of the entire substrate would be over the pins/knots - pressed against the second container).


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Offlinejigglysphinxly
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Re: Tyvek substitutes for those based outside the United States [Re: jigglysphinxly]
    #19445934 - 01/20/14 03:27 AM (10 years, 11 days ago)

Is this tek okay for cloning? LC Tek (Cloning)

It looks pretty old, but is surprisingly simple.


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Offlinemagickspore
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Re: Tyvek substitutes for those based outside the United States [Re: jigglysphinxly]
    #19446148 - 01/20/14 05:47 AM (10 years, 11 days ago)

Clone to agar.


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Offlinejigglysphinxly
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Re: Tyvek substitutes for those based outside the United States [Re: magickspore]
    #19446157 - 01/20/14 05:50 AM (10 years, 11 days ago)

Can you post a link to an agar tek? I will need to find agar in the meanwhile.
Is the LC tek useless?


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Re: Tyvek substitutes for those based outside the United States [Re: jigglysphinxly]
    #19446186 - 01/20/14 06:00 AM (10 years, 11 days ago)

Not useless. Just better with less hardy species like pans. There is quite a few negative things about LC. Its just better to start with agar.

Also I'll grab a couple links to get you started it'll be a min. I'll just edit this post with them.

Edit: Agar links. No particular order. The first two are just to give you some ideas. Also look up fast freds' agar cookbook (I think?). ALOT of recipes. Also Cronicr has some stuff done with a BRF and water(?) substitute.

http://www.shroomery.org/5243/What-are-some-good-agar-media-recipes

http://www.shroomery.org/9427/Grocery-Store-Agar-Tek

http://www.shroomery.org/forums/showflat.php/Number/19208976

http://www.shroomery.org/forums/showflat.php/Number/19249080

http://www.shroomery.org/forums/showflat.php/Number/18430976

http://www.shroomery.org/forums/showflat.php/Number/18366257


Edited by magickspore (01/20/14 06:12 AM)


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Offlinejigglysphinxly
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Re: Tyvek substitutes for those based outside the United States [Re: magickspore]
    #19446450 - 01/20/14 07:52 AM (10 years, 11 days ago)

Super thanks :smile:


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Offlinejigglysphinxly
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Re: Tyvek substitutes for those based outside the United States [Re: jigglysphinxly]
    #19475074 - 01/26/14 02:28 AM (10 years, 5 days ago)

Sorry guys for not updating. I got my harvest - around 25 ounces wet (not bad for my first attempt, and with only three quarts of MS spawn)
I ate 28g yesterday (fresh), and almost died :laugh: It was wonderful. Thanks everyone for the help - especially RR and Damion for their teks :smile:


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Offlinevikingsc
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Re: Tyvek substitutes for those based outside the United States [Re: jigglysphinxly]
    #19477521 - 01/26/14 04:14 PM (10 years, 4 days ago)

Nice. :thumbup:

I've used that cloning technique. Like he said LC's can be sketchy. If you can't find agar there is always the brf agar substitute:

http://www.shroomery.org/8514/Agar-substitute


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Offlinejigglysphinxly
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Re: Tyvek substitutes for those based outside the United States [Re: vikingsc]
    #19690392 - 03/13/14 11:25 AM (9 years, 10 months ago)

So my harvest was successful :laugh:
I had 4 flushes :cool: These pics are all from the first flush. I had plenty of side pins (I would say, 60% of my harvest was from the sides). I harvested them a little late since I wanted to see the fully grown shrooms :p

Top View:


Side View:


More Side View:


The top fruits:


Total first harvest:


Total dry weight:
First Flush: ~65gm
Second Flush: ~40gm
Third Flush: ~20gm
Fourth Flush: ~15gm

Net: 140gm dry :laugh:

Potency: Quite potent :p


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