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Willy Wonka
Medicine Man



Registered: 12/14/11
Posts: 2,109
Loc: The Other Side
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Golden Teacher Mono Tub 1
#19210787 - 12/01/13 12:20 AM (10 years, 1 month ago) |
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This is my first time working with Golden Teacher. It's a cubensis variety that I have always wanted to research. I have been holding on to two GT prints for a little while but I never started a culture because I only had two prints and I didn't want to make a syringe to find out if the print was or wasn't clean at the expense of my print being a dirty print that I lost due to contamination. So I wanted until I was comfortable with agar work.
Once I had some experience with and was confident that I could successfully work with agar I grabbed one of my two Golden Teacher prints and inoculated an agar dish in my glove box. I transferred the original culture a total of three times. My final culture was not a mono culture, but once I was sure that I was working with a clean culture I inoculated a light malt extract liquid culture with a small wedge of agar in my glove box. I also transferred a small piece of agar to a new agar dish so that I would have something to fall back on in case this project goes bad.
Since making the LC and making the fourth culture transfer I have inoculated a grain jar with a wedge from the dish which I will be using as a master grain jar to be used in my first ever grain to grain work. But that's enough about my master grain jar because that will be a new post altogether once that jar has reached 100% colonization. Now back to the LC that I made with a wedge from the third agar dish.
I think Paul Stamets identifies each new transfer from the previous culture with a "P Value" in which each new transfer gets the next higher number in the sequence of the culture. If I am wrong about this and do not understand the P value system please chime in on this thread and explain to me how the P value works. I do not want to spread wrong information. Until then I will say that the agar dish that I am working with in this grow was dish P3. I allowed the P3 Golden Teacher LC to colonize. Once the mycelium in he LC had established itself I gave it a good shake to break up the mycelium and help speed up the colonization time. On 10-11-13 I inoculated a total of four quart jars which were filled with wheat berries with about 2cc of LC each. The jars were prepared just like RogerRabbit does in his Let's Grow Mushrooms video. As of 11-29-13 only one of the jars had reached full colonization. The other three were close behind but they still haven't reached full colonization.
 In this grow I am working with a tub half the size of the standard monotub. It only needs a total of eight quarts of spawn and substrate to fill the tub up to the four inch mark. I am not 100% sure which book it is in but I am pretty sure that in "The Mushroom Cultivator: A Practical Guide to Growing Mushrooms at Home" Paul Stamets say that 10 to 1 is a good working ratio for spawn to substrate. With this in mind I decided that one quart of spawn would work just fine with seven quarts of substrate.
For this tub I went with the Damion's Elementary Coir Tek. Before I started the tek I turned my flowhood on in my grow room to help clean the air in my grow room. I let the substrate sit in the bucket for an hour then I gave it a good shake to mix everything up. After I shook the bucket I let it sit for another half hour. After letting the substrate to do it's thing for an hour and a half I dumped the substrate in the tub in front of my flowhood. I placed a meat thermometer in the tub to monitor the substrate temperature. I left the tub to sit in front of the running flowhood until the temperature of the substrate had dropped down to 100F.
 Once the temperature had dropped down to 100F. I broke up the colonized wheat in the grain jar and poured it in to the tub. I mixed it all up, put the lid on, labeled the tub with the strain, the date it was inoculated on, cracked a beer and lit a smoke. My fingers are crossed that all goes well and in about a month or so I will have plenty of fatty Golden Teacher shrooms to print and add to my print collection. Even if this tub ends up contaminated and doesn't make it to harvest I still have a clean agar culture to fall back on, and I still have three of the four LC jars colonizing. I also have the jar that I inoculated with culture P4. Either way I will get the prints I need
12-5-13:

The tub as of 12-17-13 in the morning

And here is some pin porn of the tub after I opened it on 12-17-13

12-20-13:
Edited by Willy Wonka (12/07/14 11:00 AM)
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Willy Wonka
Medicine Man



Registered: 12/14/11
Posts: 2,109
Loc: The Other Side
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Re: Golden Teacher Mono Tub [Re: Willyguci]
#19229229 - 12/04/13 10:20 PM (10 years, 1 month ago) |
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Wow I forgot about this thread and I see that it has a bunch of replies. How cool
Quote:
Willyguci said: How long did it take for it to colonize? The grain bags have been trying to colonize for 2 weeks and 2 days and no white has been seen in the grain bags. Does golden teacher take a while? What causes it to become contaminated?
The jar was inoculated via liquid culture on 10-11-13. I didn't get but one fully colonized jar out of 4 total until 11-29-13. So ya it did colonize slow, but it was colonizing in a place that was pretty cold compared to the typical 80f that cubes love. That has been fixed by adding an oil heater to the grow room that is running 24/7 right now. The heater is placed under the metal table that everything is colonizing on and the table heats to about 86f now. Contaminates are caused from mold spores that are in the air failing on top of your live cultures, or your mycology tools when they are exposed to the open air. It is also caused from mold spores that live through sterilization ie. you do not pressure cook your substrate long enough/at the proper pressure. There are other ways to introduce contamination to your grows but this covers the majority of them.
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cronicr said: great stuff, although i must say it sux to see all that work go to the bucket tek lol, I'm just bugging ya man keep it up and keep us posted
I usually use manure that I pasteurize in quart jars. I just wanted to go with the bucket tek to lessen tha chances of running into a contam. I want to re up my GT print collection before I get too crazy with contam prone things.
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FrankHorrigan said: Nice flush, WW!
I would agree with cronicr that the coir is fine in open air and there's no need to spawn in front of the flowhood.
Save your sterile technique for when it matters, like your grains and cultures.
I agree too. I just finished my flow hood though and I really wanted to use it
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Willy Wonka
Medicine Man



Registered: 12/14/11
Posts: 2,109
Loc: The Other Side
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Here is the tub as of today 12-5-13. It is colonizing really well especially for only using 1 quart jar for the entire tub. I guess the tubs really like the added heat that the heater provides
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Willy Wonka
Medicine Man



Registered: 12/14/11
Posts: 2,109
Loc: The Other Side
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Hey everyone this thread has kinda gone dead. Here is my attempt to jump some life back into it. I hadn't had the chance to get to my set up until today. Once I got to see it I was surprised to see this.
 The mushrooms are really small. I am guessing it is do to genetics. It's all good though because I am making 10 prints right now. That was the entire purpose of this grow. I needed to re-up my Golden Teacher prints and now I have 10 brand new ones to work with
And here's a little bit of pin porn to 
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Willy Wonka
Medicine Man



Registered: 12/14/11
Posts: 2,109
Loc: The Other Side
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Ok let's try this again. Hopefully I can stir up some interest in this thread again. It sort of blew up a bit but I wasn't able to get online to reply and then it died I was quite pleased to see that Frank jumped in on the thread for a second. Here is an up date with pics.
A couple shots from outside the tub:
  A couple from inside the tub:
  And two of a cute little cluster:

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Willy Wonka
Medicine Man



Registered: 12/14/11
Posts: 2,109
Loc: The Other Side
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Re: Updated the pictures up top [Re: cronicr]
#19303671 - 12/20/13 04:07 PM (10 years, 1 month ago) |
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Nice. How are you cronicr? I've seen you hitting up my posts but I haven't asked ya how you are doing. So how are ya man?
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Willy Wonka
Medicine Man



Registered: 12/14/11
Posts: 2,109
Loc: The Other Side
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Re: Updated the pictures up top [Re: Camtaro420]
#19303776 - 12/20/13 04:30 PM (10 years, 1 month ago) |
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Quote:
cronicr said: doing fucking great brutha!
Cool as shit. I am in the process of moving my set up again. I had a situation where my anonymity was compromised. But ya shit is still under control. Thanks for following. Did ya ever get anything growing with those spores? Quote:
cronicr said: got me 2 pounds of agar coming! loving life right now!
I am trying to get everything in place to do a bunch of agar work in the very near future. I am sick of multi spore work. I want to get some big fat potent ass fruits for every cube variety that I am working with and then clone that shit. I've got my flowhood set up and all. I'm just floating back and forth between 3 different spots right now. I noc one place. Then it colonizes in another. And then my tubs grow out in another. It's a PITA but it's working.
Quote:
Camtaro420 said: I hope my tubs come out as good as yours =). If only I could get some myc growing.
Just follow good teks and it will all work out. Myc work is really easy. How is your PDYA grow going right now?
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