Home | Community | Message Board

MRCA Tyroler Gluckspilze
This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Bridgetown Botanicals Bridgetown Botanicals   Left Coast Kratom Buy Kratom Extract   PhytoExtractum Maeng Da Thai Kratom Leaf Powder   Mushroom-Hut Liquid Cultures   Original Sensible Seeds Bulk Cannabis Seeds   North Spore Bulk Substrate

Jump to first unread post Pages: 1
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
InvisibleWilly Wonka
Medicine Man
 User Gallery


Registered: 12/14/11
Posts: 2,109
Loc: The Other Side Flag
Golden Teacher Mono Tub * 1
    #19210787 - 12/01/13 12:20 AM (10 years, 1 month ago)

This is my first time working with Golden Teacher.  It's a cubensis variety that I have always wanted to research.  I have been holding on to two GT prints for a little while but I never started a culture because I only had two prints and I didn't want to make a syringe to find out if the print was or wasn't clean at the expense of my print being a dirty print that I lost due to contamination.  So I wanted until I was comfortable with agar work.

    Once I had some experience with and was confident that I could successfully work with agar I grabbed one of my two Golden Teacher prints and inoculated an agar dish in my glove box.  I transferred the original culture a total of three times.  My final culture was not a mono culture, but once I was sure that I was working with a clean culture I inoculated a light malt extract liquid culture with a small wedge of agar in my glove box.  I also transferred a small piece of agar to a new agar dish so that I would have something to fall back on in case this project goes bad.

    Since making the LC and making the fourth culture transfer I have inoculated a grain jar with a wedge from the dish which I will be using as a master grain jar to be used in my first ever grain to grain work.  But that's enough about my master grain jar because that will be a new post altogether once that jar has reached 100% colonization.  Now back to the LC that I made with a wedge from the third agar dish. 

    I think Paul Stamets identifies each new transfer from the previous culture with a "P Value" in which each new transfer gets the next higher number in the sequence of the culture. If I am wrong about this and do not understand the P value system please chime in on this thread and explain to me how the P value works.  I do not want to spread wrong information.  Until then I will say that the agar dish that I am working with in this grow was dish P3.  I allowed the P3 Golden Teacher LC to colonize.  Once the mycelium in he LC had established itself I gave it a good shake to break up the mycelium and help speed up the colonization time. On 10-11-13 I inoculated a total of four quart jars which were filled with wheat berries with about 2cc of LC each.  The jars were prepared just like RogerRabbit does in his Let's Grow Mushrooms video.
   
As of 11-29-13 only one of the jars had reached full colonization.  The other three were close behind but they still haven't reached full colonization.   

   
In this grow I am working with a tub half the size of the standard monotub. It only needs a total of eight quarts of spawn and substrate to fill the tub up to the four inch mark.  I am not 100% sure which book it is in but I am pretty sure that in "The Mushroom Cultivator: A Practical Guide to Growing Mushrooms at Home" Paul Stamets say that 10 to 1 is a good working ratio for spawn to substrate.  With this in mind I decided that one quart of spawn would work just fine with seven quarts of substrate. 

    For this tub I went with the Damion's Elementary Coir Tek. Before I started the tek I turned my flowhood on in my grow room to help clean the air in my grow room.  I let the substrate sit in the bucket for an hour then I gave it a good shake to mix everything up.  After I shook the bucket I let it sit for another half hour.  After letting the substrate to do it's thing for an hour and a half I dumped the substrate in the tub in front of my flowhood.  I placed a meat thermometer in the tub to monitor the substrate temperature.  I left the tub to sit in front of the running flowhood until the temperature of the substrate had dropped down to 100F.

   
Once the temperature had dropped down to 100F.  I broke up the colonized wheat in the grain jar and poured it in to the tub.  I mixed it all up, put the lid on, labeled the tub with the strain, the date it was inoculated on, cracked a beer and lit a smoke.  My fingers are crossed that all goes well and in about a month or so I will have plenty of fatty Golden Teacher shrooms to print and add to my print collection.  Even if this tub ends up contaminated and doesn't make it to harvest I still have a clean agar culture to fall back on, and I still have three of the four LC jars colonizing.  I also have the jar that I inoculated with culture P4.  Either way I will get the prints I need:thumbup:

12-5-13:


The tub as of 12-17-13 in the morning


And here is some pin porn of the tub after I opened it on 12-17-13


12-20-13:


Edited by Willy Wonka (12/07/14 11:00 AM)


Extras: Unfilter Print Post Top
InvisibleFrankHorrigan
The Inquisition
Male


Registered: 01/04/11
Posts: 10,573
Trusted Cultivator
Re: Golden Teacher Mono Tub [Re: Pastywhyte]
    #19226629 - 12/04/13 01:08 PM (10 years, 1 month ago)

:whathesaid:

Plus, each jar is an opportunity for a loss.  So I try to minimize the number of containers I need to use at a given time.

Everything I do starts with live culture on an agar wedge.

My master quart jars colonize in 2 weeks at 81F. 

I can fit 56 of these jars in my 35 gallon tub I use for incubation.


Each master jar is G2G'd into a filter bag containing 5 more quarts of grain. This colonizes in 7-10 days, tops, at 72F.

I can fit six of these bags into a single 35 gallon tub for colonizing.


With these time frames, I go from culture to harvest in about 5-6 weeks, running 10-20 monos a month.

I think I'm pretty set with my "large" jars :lol:


--------------------

Yes, you can bump my old threads with a question.
Here is how I get things done.
You should take a look. :hehehe:


Frank's tips and tricks. Updated on 3/21/14
AMU- Get an answer here -AMU


Extras: Unfilter Print Post Top
InvisibleWilly Wonka
Medicine Man
 User Gallery


Registered: 12/14/11
Posts: 2,109
Loc: The Other Side Flag
Updated Tub Picture [Re: Willy Wonka]
    #19231035 - 12/05/13 12:33 PM (10 years, 1 month ago)

Here is the tub as of today 12-5-13.  It is colonizing really well especially for only using 1 quart jar for the entire tub.  I guess the tubs really like the added heat that the heater provides:willynilly:


--------------------
AMU



Extras: Unfilter Print Post Top
InvisibleWilly Wonka
Medicine Man
 User Gallery


Registered: 12/14/11
Posts: 2,109
Loc: The Other Side Flag
Updated up top [Re: Willy Wonka]
    #19290472 - 12/17/13 08:27 PM (10 years, 1 month ago)

Hey everyone this thread has kinda gone dead.  Here is my attempt to jump some life back into it.  I hadn't had the chance to get to my set up until today.  Once I got to see it I was surprised to see this.

The mushrooms are really small.  I am guessing it is do to genetics.  It's all good though because I am making 10 prints right now.  That was the entire purpose of this grow.  I needed to re-up my Golden Teacher prints and now I have 10 brand new ones to work with:thumbup:

And here's a little bit of pin porn to :whacker:


--------------------
AMU



Extras: Unfilter Print Post Top
InvisibleWilly Wonka
Medicine Man
 User Gallery


Registered: 12/14/11
Posts: 2,109
Loc: The Other Side Flag
Updated the pictures up top [Re: Willy Wonka]
    #19303627 - 12/20/13 03:57 PM (10 years, 1 month ago)

Ok let's try this again.  Hopefully I can stir up some interest in this thread again.  It sort of blew up a bit but I wasn't able to get online to reply and then it died:goodnight:  I was quite pleased to see that Frank jumped in on the thread for a second.  Here is an up date with pics.

A couple shots from outside the tub:

A couple from inside the tub:

And two of a cute little cluster:


--------------------
AMU



Extras: Unfilter Print Post Top
Jump to top Pages: 1

Shop: Bridgetown Botanicals Bridgetown Botanicals   Left Coast Kratom Buy Kratom Extract   PhytoExtractum Maeng Da Thai Kratom Leaf Powder   Mushroom-Hut Liquid Cultures   Original Sensible Seeds Bulk Cannabis Seeds   North Spore Bulk Substrate


Similar ThreadsPosterViewsRepliesLast post
* First Time Grow Log (Golden Teacher)
( 1 2 3 all )
abaccusx 37,578 42 05/20/05 08:17 AM
by abaccusx
* How tall should the fruiting chamber be for Golden Teachers? MikeHuntShrooms 1,912 9 01/14/04 01:45 PM
by Che_Night_Soil
* Golden Teacher Vs. Mexicub Firewolf1327 7,508 14 04/02/05 10:26 AM
by PawPaw
* Here are some more pics of my Golden Teacher pins...
( 1 2 all )
caspur 5,372 22 05/04/04 09:15 PM
by caspur
* Re: PICS of Golden Teacher casing phrozendata 18,107 7 06/30/00 11:30 PM
by MuffinBear
* Golden Teacher?? foxracinfan567 7,866 11 02/18/10 12:43 PM
by Roadkill
* Slow Golden Teacher/Texas Growth (Advice pls) Pr0_X 4,994 6 06/09/06 03:11 PM
by monstermitch
* Mono-Tub vs Double Tub
( 1 2 all )
unknown28 14,253 34 12/17/23 02:13 PM
by TrippHillHooligan

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
10,294 topic views. 19 members, 146 guests and 32 web crawlers are browsing this forum.
[ Show All Posts | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.024 seconds spending 0.007 seconds on 16 queries.