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EarthBounded
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Waiting 24 hours to sterilize WBS / Time
#19209361 - 11/30/13 05:25 PM (10 years, 1 month ago) |
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Ahhh nothing but ups and downs here are my two questions to help me sleep at night. I am using doc34s was tek:
First off after my 15psi weight started to wobble we only were able to run them on low for 45 minutes not 60 because we needed time to depressurize before making it to holiday diner, will this put me at major risk for con tams, we soaked and rinsed and did ever step perfect, the other thing is we did not have time to do the other 7 quart jars we have all ready to be put in the PC. We are storing these in a cool room and they are covered in tinfoil and we will sterilize tomorrow will this 24 gap from jarring til it actually makes it to the PC be an issue?
I also Knocked up a two pound rye bag and 6 brf bags just for a fun holiday season.
Fun day 
I am hoping the WBS colonizes fast then spawning to franks simple coir in 14 quart containers. Do you think 1 quart of spawn is good for a 14 qt mini mono?
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Pastywhyte
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Re: Waiting 24 hours to sterilize WBS / Time [Re: EarthBounded]
#19209396 - 11/30/13 05:36 PM (10 years, 1 month ago) |
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I use an 18 quart mini mono for experiments and ms grows. I usually like to go 1.5 - 2 quarts spawn at a 1:2 ratio. You could probably get away with 1 quart, but more would be better IMO. However I use rye which has less nock points so maybe with WBS you might see faster colonization Giver a try, should work okay might just take a little longer for the spawn run.
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OgreLokon
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Re: Waiting 24 hours to sterilize WBS / Time [Re: Pastywhyte]
#19209464 - 11/30/13 05:56 PM (10 years, 1 month ago) |
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If you have more jars to do anyway, why don't you just sterilize them all at once? Better safe than sorry!
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teknix
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Re: Waiting 24 hours to sterilize WBS / Time [Re: EarthBounded]
#19209483 - 11/30/13 06:01 PM (10 years, 1 month ago) |
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Quote:
EarthBounded said: Ahhh nothing but ups and downs here are my two questions to help me sleep at night. I am using doc34s was tek:
First off after my 15psi weight started to wobble we only were able to run them on low for 45 minutes not 60 because we needed time to depressurize before making it to holiday diner, will this put me at major risk for con tams, we soaked and rinsed and did ever step perfect, the other thing is we did not have time to do the other 7 quart jars we have all ready to be put in the PC. We are storing these in a cool room and they are covered in tinfoil and we will sterilize tomorrow will this 24 gap from jarring til it actually makes it to the PC be an issue?
I also Knocked up a two pound rye bag and 6 brf bags just for a fun holiday season.
Fun day 
I am hoping the WBS colonizes fast then spawning to franks simple coir in 14 quart containers. Do you think 1 quart of spawn is good for a 14 qt mini mono?
I've had success with as little PC time as 30 mins, it sort of just depends on whether or not there are endospores hiding in a dry spot of your grains, if you prepared them properly and they were fully saturated then the endospores probably already germinated.
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Kizzle
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Re: Waiting 24 hours to sterilize WBS / Time [Re: teknix]
#19209574 - 11/30/13 06:23 PM (10 years, 1 month ago) |
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You have to heat shock endospores if you're trying to germinate them. A simple cold soak will only cause a relatively small amount to germinate at a time. 60 minutes is cutting it slim already, I would not rely on 45 minutes. That's not even long enough for center grains to reach sterilization temperatures.
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teknix
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Re: Waiting 24 hours to sterilize WBS / Time [Re: Kizzle]
#19209600 - 11/30/13 06:28 PM (10 years, 1 month ago) |
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Don't you heat the grains prior to pressure cooking them?
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PussyFart
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Re: Waiting 24 hours to sterilize WBS / Time [Re: teknix]
#19209618 - 11/30/13 06:31 PM (10 years, 1 month ago) |
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Quote:
teknix said: Don't you heat the grains prior to pressure cooking them?
Yes, and then you usually let them sit and strain for 30 minutes to an hour.....you really think that they are still warm?
Grains get PCed for no less than 90 minutes at 15psi.....we are not just saying this to waste our time.
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teknix
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Re: Waiting 24 hours to sterilize WBS / Time [Re: PussyFart]
#19209627 - 11/30/13 06:32 PM (10 years, 1 month ago) |
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Quote:
Notahacker420 said:
Quote:
teknix said: Don't you heat the grains prior to pressure cooking them?
Yes, and then you usually let them sit and strain for 30 minutes to an hour.....you really think that they are still warm?
Grains get PCed for no less than 90 minutes at 15psi.....we are not just saying this to waste our time.
What? Sorry you waste so much time pcing?
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Pastywhyte
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Re: Waiting 24 hours to sterilize WBS / Time [Re: teknix]
#19209677 - 11/30/13 06:42 PM (10 years, 1 month ago) |
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Its not a waste to get clean spawn. . .
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PussyFart
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Re: Waiting 24 hours to sterilize WBS / Time [Re: teknix]
#19209688 - 11/30/13 06:46 PM (10 years, 1 month ago) |
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Quote:
teknix said: What? Sorry you waste so much time pcing?
I usually let them go for 100-110 minutes......it's not a waste....it's proper sterilization.
You might not mind the occasional contam.....I do.
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THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT! A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD, SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List, EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE *** *** AFGHAN KUSH GROW LOG *** ***
Edited by PussyFart (11/30/13 06:46 PM)
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teknix
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Re: Waiting 24 hours to sterilize WBS / Time [Re: PussyFart]
#19209756 - 11/30/13 07:00 PM (10 years, 1 month ago) |
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It is sterilized when the center reaches 250 for 15 mins, after that it is a waste.
Quote:
A widely used method for heat sterilization is the autoclave, sometimes called a converter. Autoclaves commonly use steam heated to 121β134 Β°C (250β273 Β°F). To achieve sterility, a holding time of at least 15 minutes at 121 Β°C (250 Β°F) at 100 kPa (15 psi), or 3 minutes at 134 Β°C (273 Β°F) at 100 kPa (15 psi) is required.
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PussyFart
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Re: Waiting 24 hours to sterilize WBS / Time [Re: teknix]
#19209780 - 11/30/13 07:07 PM (10 years, 1 month ago) |
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Quote:
teknix said: It is sterilized when the center reaches 250 for 15 mins, after that it is a waste.
And how long does it take for the center of everyone's jars(not just yours) to reach that temp?
I mean unless you actually have a thermometer in one of the jars inside the PC(and then be able to see it somehow), there is no way you can sit here and tell me that 45 minutes is sufficient to get the center to proper temp.
You are not going to convince anybody that 45 minutes is long enough for grains......we sterilize cakes for longer.....
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THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT! A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD, SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List, EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE *** *** AFGHAN KUSH GROW LOG *** ***
Edited by PussyFart (11/30/13 07:08 PM)
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teknix
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Re: Waiting 24 hours to sterilize WBS / Time [Re: PussyFart]
#19209790 - 11/30/13 07:09 PM (10 years, 1 month ago) |
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Not as long as you might think under 15 psi I suppose.
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teknix
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Re: Waiting 24 hours to sterilize WBS / Time [Re: PussyFart]
#19209793 - 11/30/13 07:11 PM (10 years, 1 month ago) |
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Quote:
Notahacker420 said:
Quote:
teknix said: It is sterilized when the center reaches 250 for 15 mins, after that it is a waste.
And how long does it take for the center of everyone's jars(not just yours) to reach that temp?
I mean unless you actually have a thermometer in one of the jars inside the PC(and then be able to see it somehow), there is no way you can sit here and tell me that 45 minutes is sufficient to get the center to proper temp.
You are not going to convince anybody that 45 minutes is long enough for grains......we sterilize cakes for longer.....
You don't need to sterilize cakes, in fact you can make them in the oven or boiling water.
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Re: Waiting 24 hours to sterilize WBS / Time [Re: teknix]
#19209799 - 11/30/13 07:12 PM (10 years, 1 month ago) |
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Quote:
teknix said: You don't need to sterilize cakes, in fact you can make them in the oven
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teknix
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Re: Waiting 24 hours to sterilize WBS / Time [Re: Pastywhyte]
#19209804 - 11/30/13 07:14 PM (10 years, 1 month ago) |
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Have you guys ever tried it?
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PussyFart
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Re: Waiting 24 hours to sterilize WBS / Time [Re: teknix]
#19209806 - 11/30/13 07:15 PM (10 years, 1 month ago) |
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Quote:
teknix said: You don't need to sterilize cakes, in fact you can make them in the oven or boiling water.
And how long do you boil steam cakes for?
You do realize that using an oven or steaming them still sterilizes them right?
Except using an oven is just going to ruin your moisture content....
Heat sterilizes....whether it be heat from steam or a PC....it all gets you to the same place....please stop while you are behind.....
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THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT! A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD, SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List, EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE *** *** AFGHAN KUSH GROW LOG *** ***
Edited by PussyFart (11/30/13 07:15 PM)
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teknix
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Re: Waiting 24 hours to sterilize WBS / Time [Re: PussyFart]
#19209809 - 11/30/13 07:15 PM (10 years, 1 month ago) |
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No it doesn't, you need pressure to sterilize.
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PussyFart
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Re: Waiting 24 hours to sterilize WBS / Time [Re: teknix]
#19209820 - 11/30/13 07:17 PM (10 years, 1 month ago) |
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Quote:
teknix said: No it doesn't, you need pressure to sterilize.
And you need to do some research.....you do not need pressure to sterilize...just heat....you could steam grains for 8 hours and get them sterile....or PC for 90 minutes.....you sound like a child.....
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THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT! A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD, SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List, EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE *** *** AFGHAN KUSH GROW LOG *** ***
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teknix
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Re: Waiting 24 hours to sterilize WBS / Time [Re: PussyFart]
#19209823 - 11/30/13 07:18 PM (10 years, 1 month ago) |
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Rofl, I'm a biologist.
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PussyFart
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Re: Waiting 24 hours to sterilize WBS / Time [Re: teknix]
#19209826 - 11/30/13 07:19 PM (10 years, 1 month ago) |
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Quote:
teknix said: Rofl, I'm a biologist.
Rofl you're a moron......
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THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT! A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD, SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List, EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE *** *** AFGHAN KUSH GROW LOG *** ***
Edited by PussyFart (11/30/13 07:19 PM)
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Pastywhyte
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Re: Waiting 24 hours to sterilize WBS / Time [Re: teknix]
#19209829 - 11/30/13 07:20 PM (10 years, 1 month ago) |
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Quote:
teknix said: Rofl, I'm a biologist.
"Trust me, I'm a doctor"
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teknix
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Re: Waiting 24 hours to sterilize WBS / Time [Re: PussyFart]
#19209835 - 11/30/13 07:23 PM (10 years, 1 month ago) |
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A moron with a Bio degree.






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PussyFart
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Re: Waiting 24 hours to sterilize WBS / Time [Re: teknix]
#19209850 - 11/30/13 07:26 PM (10 years, 1 month ago) |
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And who says....
Quote:
teknix said: You don't need to sterilize cakes, in fact you can make them in the oven or boiling water.

Why would you boil them if they do not have to be sterilized? Why use an oven? Why use heat?
What exactly does that step do? It sterilizes them.....duh.
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THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT! A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD, SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List, EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE *** *** AFGHAN KUSH GROW LOG *** ***
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teknix
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Re: Waiting 24 hours to sterilize WBS / Time [Re: PussyFart]
#19209856 - 11/30/13 07:27 PM (10 years, 1 month ago) |
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It kills the bacteria that would likely grow on the surface of the substratum rather than within it. Flour is mostly surface area and can't really hide much.
Also did you read the definition of sterilization?
Quote:
(15 psi) is required
Edited by teknix (11/30/13 07:39 PM)
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PussyFart
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Re: Waiting 24 hours to sterilize WBS / Time [Re: teknix]
#19209863 - 11/30/13 07:31 PM (10 years, 1 month ago) |
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Quote:
teknix said: It kills the bacteria that would likely grow on the surface of the substratum rather than within it.
Ok...sure bro......I want what you're smokin....
And WTF is "the substratum"?
I am not a biologist or a mycologist.. and I think you mean substrate.
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THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT! A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD, SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List, EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE *** *** AFGHAN KUSH GROW LOG *** ***
Edited by PussyFart (11/30/13 07:31 PM)
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teknix
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Re: Waiting 24 hours to sterilize WBS / Time [Re: PussyFart]
#19209868 - 11/30/13 07:32 PM (10 years, 1 month ago) |
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Yeah I mean substrate.
subΒ·straΒ·tum (sbstrtm, -strtm) n. pl. subΒ·straΒ·ta (-strt, -strt) or subΒ·straΒ·tums
5. Biology A substrate.
http://www.thefreedictionary.com/substratum
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PussyFart
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Re: Waiting 24 hours to sterilize WBS / Time [Re: teknix]
#19209873 - 11/30/13 07:33 PM (10 years, 1 month ago) |
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I know......
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THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT! A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD, SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List, EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE *** *** AFGHAN KUSH GROW LOG *** ***
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teknix
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Re: Waiting 24 hours to sterilize WBS / Time [Re: PussyFart]
#19209877 - 11/30/13 07:35 PM (10 years, 1 month ago) |
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It is viable to put a bunch of cake mix in an aluminum pan and cook it in the oven and spawn to it without contams.
If you made the cakes the same way and used them as spawn then you just grew a shit ton of mushrooms without a PC.
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PussyFart
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Re: Waiting 24 hours to sterilize WBS / Time [Re: teknix]
#19209884 - 11/30/13 07:37 PM (10 years, 1 month ago) |
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Quote:
teknix said: You can viably put a bunch of cake mix in an aluminum pan and cook it in the oven and spawn to it without contams.
I am done with you......I cannot deal with such bullshit nonsense.....you top the cake.....
Someone else get this please......
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THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT! A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD, SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List, EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE *** *** AFGHAN KUSH GROW LOG *** ***
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teknix
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Re: Waiting 24 hours to sterilize WBS / Time [Re: PussyFart]
#19209888 - 11/30/13 07:38 PM (10 years, 1 month ago) |
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Not my fault you take peoples word for it rather than experimenting.
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PussyFart
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Re: Waiting 24 hours to sterilize WBS / Time [Re: teknix]
#19209898 - 11/30/13 07:40 PM (10 years, 1 month ago) |
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Quote:
teknix said: Not my fault you take peoples word for it rather than experimenting.
Until you have done it AT LEAST 100 times in a row without failure, please do not post such nonsense on here.....
Anything can happen just once....but repeated success is the key.....spawning to BRF "Cooked"(not sterilized) in an oven is not what I would call experiment worthy.....
It's almost like you are trying to troll.....
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THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT! A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD, SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List, EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE *** *** AFGHAN KUSH GROW LOG *** ***
Edited by PussyFart (11/30/13 07:41 PM)
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Re: Waiting 24 hours to sterilize WBS / Time [Re: PussyFart]
#19209905 - 11/30/13 07:42 PM (10 years, 1 month ago) |
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Replicate it then and find out.
Actually, you would probably screw it up without a proper procedure or tech to read step by step, so don't bother because you will use your own inadequacy as evidence that it doesn't work.
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nn-IlliniSpiralDMT
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Re: Waiting 24 hours to sterilize WBS / Time [Re: PussyFart] 1
#19209914 - 11/30/13 07:43 PM (10 years, 1 month ago) |
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Quote:
Notahacker420 said:
Quote:
teknix said: Not my fault you take peoples word for it rather than experimenting.
Until you have done it AT LEAST 100 times in a row without failure, please do not post such nonsense on here.....
Anything can happen just once....but repeated success is the key.....spawning to BRF "Cooked"(not sterilized) in an oven is not what I would call experiment worthy.....
It's almost like you are trying to troll.....
Both of you are children...
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PussyFart
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But at least I come across as being older than eleventeen.....
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THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT! A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD, SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List, EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE *** *** AFGHAN KUSH GROW LOG *** ***
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Re: Waiting 24 hours to sterilize WBS / Time [Re: PussyFart]
#19209929 - 11/30/13 07:46 PM (10 years, 1 month ago) |
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Not really. You're prob like 18 or 19.
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nn-IlliniSpiralDMT
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Re: Waiting 24 hours to sterilize WBS / Time [Re: PussyFart]
#19209936 - 11/30/13 07:47 PM (10 years, 1 month ago) |
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Quote:
Notahacker420 said: But at least I come across as being older than eleventeen.....
I'll give you eleventeen and half
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teknix
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Re: Waiting 24 hours to sterilize WBS / Time [Re: PussyFart]
#19209943 - 11/30/13 07:49 PM (10 years, 1 month ago) |
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Quote:
Notahacker420 said:
Quote:
teknix said: You can viably put a bunch of cake mix in an aluminum pan and cook it in the oven and spawn to it without contams.
I am done with you......I cannot deal with such bullshit nonsense.....you top the cake.....
Someone else get this please......
I told you one of the greatest secrets to growing shrooms, and you don't even appreciate it.
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PussyFart
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Re: Waiting 24 hours to sterilize WBS / Time [Re: teknix]
#19209944 - 11/30/13 07:49 PM (10 years, 1 month ago) |
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--------------------
THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT! A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD, SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List, EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE *** *** AFGHAN KUSH GROW LOG *** ***
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Pastywhyte
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Re: Waiting 24 hours to sterilize WBS / Time [Re: teknix]
#19209945 - 11/30/13 07:49 PM (10 years, 1 month ago) |
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teknix said: Not my fault you take peoples word for it rather than experimenting.
I love a good experiment but we have posts on here by the thousands of people who half assed there sterilization and have nothing but contams to show for it.
Here in cultivation we encourage experiments as long as said procedures are presented as such and not encouraged as fact. What you propose sounds pretty half baked and by no stretch should be encouraged. Maybe you made a cake in a oven. Maybe you grew cubes on a raw steak. Maybe you cultivated pans on the moon. It don't matter without pics or proof that the experiment can be replicated many times, and without those two things no one is going to appreciate you spreading that kind of poor info to be corrected a 1000 times down the road.
We advise noobs to stick to a proven tek, not because we're dicks who hate experiments, but because were gonna have 1000 noobs and 10000 lurkers read this thread and then complain that they grew some mold
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teknix
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Re: Waiting 24 hours to sterilize WBS / Time [Re: Pastywhyte]
#19209952 - 11/30/13 07:52 PM (10 years, 1 month ago) |
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Try it then, if you figure it out then write it up, unless you are the noob you are referencing.
It's simple, get an aluminum pan and put in the sub/cake mix (Rye or Wheat flour pref) about 1/3 of the way up, cover it aluminum foil and heat it in the oven to 350, once it reaches 350, turn in down to 230-250 and wait an hour.
You can also throw the casing (covered) in the oven with it for the next step.
Let it cool down, put it in your glove box or under a flow-hood and spawn to it. Cover the top layer of colonized sub with a casing, then re-cover it with the aluminum foil making sure it is sealed all the way around. Put the pan in a plastic grocery bag and tie it shut.
Wait a couple to a few weeks and fruit it.
ezpz
Edited by teknix (11/30/13 08:18 PM)
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lessismore
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Re: Waiting 24 hours to sterilize WBS / Time [Re: EarthBounded]
#19209968 - 11/30/13 07:56 PM (10 years, 1 month ago) |
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Quote:
EarthBounded said: Ahhh nothing but ups and downs here are my two questions to help me sleep at night. I am using doc34s was tek:
First off after my 15psi weight started to wobble we only were able to run them on low for 45 minutes not 60 because we needed time to depressurize before making it to holiday diner, will this put me at major risk for con tams, we soaked and rinsed and did ever step perfect, the other thing is we did not have time to do the other 7 quart jars we have all ready to be put in the PC. We are storing these in a cool room and they are covered in tinfoil and we will sterilize tomorrow will this 24 gap from jarring til it actually makes it to the PC be an issue?
I also Knocked up a two pound rye bag and 6 brf bags just for a fun holiday season.
Fun day 
I am hoping the WBS colonizes fast then spawning to franks simple coir in 14 quart containers. Do you think 1 quart of spawn is good for a 14 qt mini mono?
Where are your nutrients gonna come from?
Mushrooms need both water and nutrients, the more quarts of spawn the better IMO
Coir is good due to water retention and texture, then you just need lots of nutrients people often use 6 quarts or so of nutrients for monos
The more nutrients you add, the fewer monos you have to do, and the higher first yield :-)
One day I hope to try coir+wormcastings+6 wbs Need about 4" of sub
24-36 hours seems to have worked for me, but the tek recommends less than that (it will just ferment a bit, but still useable most of the time) I also shake the jars after taking them out of the PC, so the WBS doesnt stick to the sides
Edited by lessismore (11/30/13 08:04 PM)
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Pastywhyte
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Re: Waiting 24 hours to sterilize WBS / Time [Re: teknix]
#19209971 - 11/30/13 07:57 PM (10 years, 1 month ago) |
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Quote:
teknix said: Try it then, if you figure it out then write it up, unless you are the noob you are referencing.
I would love to but at the moment I gotta a lot of other ill advised experiments on the go. Things that I want to test before crashing into someone else's thread spouting off like I was giving the mycological sermon on the mount. Since this is your brainchild, the burden of proof would be on you to prove your claims. In the meantime the rest of us will grow mushies using time tested methods and I will continue with my own experiments on the side.
Oh and FYI I am a noob. Probably always will be.
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teknix
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Re: Waiting 24 hours to sterilize WBS / Time [Re: Pastywhyte]
#19209982 - 11/30/13 08:00 PM (10 years, 1 month ago) |
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What do you want proof of? The concept is solid, there is no reason it wouldn't work.
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lessismore
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Re: Waiting 24 hours to sterilize WBS / Time [Re: teknix]
#19210009 - 11/30/13 08:08 PM (10 years, 1 month ago) |
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btw, I saw a tray with coir+2 pf cakes.. 150g wet, or 15g dry 1st flush iirc (2pf cakes is approx same volume spawn as 1 quart WBS iirc)
it was in a tek in here somewhere
if you are happy with that yield I would go ahead with a mini mono, mini monos work great IME (although I really prefer full sized monos, tend to do much better, maybe due to better airflow?) have had around 150-170g max 1st flush from 2 pfcakes too with straw in a normal sized mono (but the shrooms were weak, maybe due to not a lot of nutrients vs bulk sub.. not sure)
cant get enough spawn now :-) need a larger PC
Edited by lessismore (11/30/13 08:15 PM)
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Pastywhyte
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Re: Waiting 24 hours to sterilize WBS / Time [Re: teknix]
#19210047 - 11/30/13 08:18 PM (10 years, 1 month ago) |
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Quote:
teknix said: What do you want proof of? The concept is solid, there is no reason it wouldn't work.
To be frank, it sounds like a pain in the ass. I use agar and grains because they are proven, with a low fail rate. I'm not worried about growing mushrooms without a PC. No end game in that idea for me, sorry
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teknix
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Re: Waiting 24 hours to sterilize WBS / Time [Re: Pastywhyte]
#19210055 - 11/30/13 08:19 PM (10 years, 1 month ago) |
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It's probably the least messy way to get a tray going, because you don't need so many containers to clean.
You only have the spawn jars/bags and the casing tray to worry about. And generally you can fit way more stuff into your oven then you can into a PC.
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PussyFart
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Re: Waiting 24 hours to sterilize WBS / Time [Re: teknix]
#19210060 - 11/30/13 08:22 PM (10 years, 1 month ago) |
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When you say cakes, you mean BRF cakes or bulk substrate in trays?
--------------------
THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT! A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD, SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List, EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE *** *** AFGHAN KUSH GROW LOG *** ***
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teknix
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Re: Waiting 24 hours to sterilize WBS / Time [Re: PussyFart]
#19210062 - 11/30/13 08:23 PM (10 years, 1 month ago) |
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Flour + verm, Or Flour + activated carbon, or a combination.
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PussyFart
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Re: Waiting 24 hours to sterilize WBS / Time [Re: teknix]
#19210070 - 11/30/13 08:27 PM (10 years, 1 month ago) |
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I thought so.....that has fail written all over it......
I was thinking maybe you were confusing terms, but no, it's just a wack experiment....BRF needs to be kept sterile....it is not used as a bulk substrate additive for this reason.
Spawning to BRF in open air is just asking for contams IMO....regardless of the sterilization method.
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THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT! A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD, SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List, EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE *** *** AFGHAN KUSH GROW LOG *** ***
Edited by PussyFart (11/30/13 08:28 PM)
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teknix
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Re: Waiting 24 hours to sterilize WBS / Time [Re: PussyFart]
#19210080 - 11/30/13 08:34 PM (10 years, 1 month ago) |
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Open air? There is no open air mang.
If you don't have a flow hood and can't fir your trays in a glovebox then get a bigger glove box.
You could make a bunch of cake trays and put them into a monotub if you had a flow hood and cleaned out the tubs really well, or had a nice little area to decontaminate.
The casing layer and grains will protect the nutrients at the bottom, like the layer of verm protects your cakes.
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lessismore
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Re: Waiting 24 hours to sterilize WBS / Time [Re: teknix]
#19210098 - 11/30/13 08:39 PM (10 years, 1 month ago) |
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I would probably not spawn to BRF in openair/glovebox, sounds dangerous
have you had success with that?
with straw I put my BRF cakes in the blender and mix with straw in open air though but that is because they colonize so quickly and the straw is pasteurized to not really allow molds, pasteurization preserves the helpful bacteria so molds will have a long time to take a hold of it the first many days and all the spawn is already 100% colonized
not so if you spawn to uncolonized BRF thats just a black/green mold infestation waiting to happen IMO, even in a glovebox
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teknix
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Re: Waiting 24 hours to sterilize WBS / Time [Re: teknix]
#19210100 - 11/30/13 08:39 PM (10 years, 1 month ago) |
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The part to worry about in making a tub this way is when you first spawn the cake or grains.
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Hammburgler
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Re: Waiting 24 hours to sterilize WBS / Time [Re: PussyFart]
#19210104 - 11/30/13 08:40 PM (10 years, 1 month ago) |
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yeah this cook it in the oven in a pan stuff is full of failure. the first and not even the least of the problem is that the outer edges of the substrate would most likely burn being as they are in direct contact with a conductive metal. Second is that it does not require sufficient pressure to kill all of the microbes or spores. You can, however, steam sterilize over the course of 3 days with 3 steam treatments. These allow for the germination of endospores over the course of that time and subsequently allow them to be killed.
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teknix
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Re: Waiting 24 hours to sterilize WBS / Time [Re: lessismore]
#19210105 - 11/30/13 08:40 PM (10 years, 1 month ago) |
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Quote:
mio said: I would probably not spawn to BRF in openair/glovebox, sounds dangerous
have you had success with that?
with straw I put my BRF cakes in the blender and mix with straw in open air though but that is because they colonize so quickly and the straw is pasteurized to not really allow molds, pasteurization preserves the helpful bacteria so molds will have a long time to take a hold of it the first many days
Yeah, most of my work in the lab is open air inoculation. I have never even turned the flow-hood on, and we don't have a glove box. But peoples houses aren't generally as clean, so I wouldn't do a tub unless you are sure there is a clean place to do it, and that would be a lot of wasted materials if you screwed it up. But you can do it.
Edited by teknix (11/30/13 08:49 PM)
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teknix
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Re: Waiting 24 hours to sterilize WBS / Time [Re: Hammburgler]
#19210127 - 11/30/13 08:46 PM (10 years, 1 month ago) |
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Quote:
Hammburgler said: yeah this cook it in the oven in a pan stuff is full of failure. the first and not even the least of the problem is that the outer edges of the substrate would most likely burn being as they are in direct contact with a conductive metal. Second is that it does not require sufficient pressure to kill all of the microbes or spores. You can, however, steam sterilize over the course of 3 days with 3 steam treatments. These allow for the germination of endospores over the course of that time and subsequently allow them to be killed.
Nah, it is all wet, no burning, the foil keeps the water inside and steams the sub, it doesn't leak out if you make sure it is sealed properly. It comes out pretty much the same consistency as it went it.
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teknix
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Re: Waiting 24 hours to sterilize WBS / Time [Re: teknix]
#19210146 - 11/30/13 08:52 PM (10 years, 1 month ago) |
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If you want to do a tub, you should probably use cardboard, and put the cake mix in the tub while it's hot and cover it with pastuerized/boiled cardboard, until it cools down, then spawn the grains on top of the cardboard and case over it.
The cardboard can protect the nutritive sub from being exposed to air.
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EarthBounded
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Re: Waiting 24 hours to sterilize WBS / Time [Re: teknix]
#19210410 - 11/30/13 10:20 PM (10 years, 1 month ago) |
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You are all strait cray, YES I love these responses because I learn a shit load. NotHacker420 teknix Mio
I love all of you for the info, I don't give a fuck who is right or wrong I learned what I needed, I am going to inoculate them all in the morning then follow up.
Also I didn't get much response just from MIO on my jars that had to sit for like 12-24 hours before being PCd huge problem?
Even tho they were only PCd for 45 minutes after the weight wobbled I still feel we created sterile spawn we will see, I am the king of messing up around here.
I have a pretty cheap PC too so it was on for a good hour and 15 min but only 45 min after it showed 15psi
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Edited by EarthBounded (11/30/13 10:35 PM)
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EarthBounded
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Re: Waiting 24 hours to sterilize WBS / Time [Re: EarthBounded]
#19212043 - 12/01/13 11:02 AM (10 years, 1 month ago) |
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Bump,
Need to know kinda quick, I didn't get much response, we washed ,soaked and simmered our wbs, tyveked ,poly filled and put tinfoil over these last night but we had a concert to go to so we were not able to sterilize until this morning is that a problem to leave the jars in a cold room covererd for 24 hours in between loading and PCing.
thx EB
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Juicin
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Re: Waiting 24 hours to sterilize WBS / Time [Re: EarthBounded]
#19212128 - 12/01/13 11:21 AM (10 years, 1 month ago) |
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Ok so two things, the first may be unnecessary
The goal is to get your PC up to 15 psi and leave it there for 1.5 hours. Doesn't matter how long it takes to get there, you need 1.5 hours at 15 psi. Once it comes up to pressure you lower your heat until the rocker is just barely spitting steam. You only start the timer after you get the PC to pressure
On the second point it seems like you already PC'd for 45 minutes? On it's face I can see no reason why you wouldn't be able to pop them back in, unless the moisture content is fucked up. But if you have the cash and limited spores I would just re do it all. I know that sucks but if something goes wrong you don't want to think "DAMNIT I SHOULD HAVE JUST MADE NEW SPAWN JARS".
Doing a step perfectly allows you to discount it as a problem if things go wrong down the line.
edit - words
Edited by Juicin (12/01/13 11:26 AM)
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FrankHorrigan
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Re: Waiting 24 hours to sterilize WBS / Time [Re: EarthBounded]
#19212368 - 12/01/13 12:08 PM (10 years, 1 month ago) |
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Quote:
EarthBounded said: Bump,
Need to know kinda quick, I didn't get much response, we washed ,soaked and simmered our wbs, tyveked ,poly filled and put tinfoil over these last night but we had a concert to go to so we were not able to sterilize until this morning is that a problem to leave the jars in a cold room covererd for 24 hours in between loading and PCing.
thx EB
You can leave the jars for days before PC'ing and still get away with it, 24 hours won't hurt a thing.
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