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OfflineNicodArleone
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Registered: 12/31/19
Posts: 59
Last seen: 3 years, 19 days
Re: Pastywhyte's Easy Agar Tek [Re: lord_nikon6983]
    #26955768 - 09/26/20 08:59 AM (3 years, 8 months ago)

Quote:

lord_nikon6983 said:
By tools you mean the needle? I dont have any experience with them but I know that its pretty common to use self healing injection ports on jars. I figured that if I flamed the needle and then wiped the injection site with an alcohol wipe before pushing the needle through this would expose the plate itself to less possibility of contamination compared to opening the container...


I have 6 plates ready... maybe I'll experiment and do 3 through the tape and 3 by opening them.

I would definitely appreciate some more opinions.

Thanks




I just excessive cleaning.

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Offlinenothanksman

Registered: 09/25/20
Posts: 76
Last seen: 11 months, 10 days
Re: Pastywhyte's Easy Agar Tek [Re: NicodArleone]
    #26963405 - 10/01/20 07:54 AM (3 years, 8 months ago)

I know this thread is old af, but I gotta get some posts in and it'll be easier to find later if I comment. Thanks for the write up.

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OfflineThorasta
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Registered: 03/21/18
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Re: Pastywhyte's Easy Agar Tek [Re: nothanksman]
    #26964166 - 10/01/20 03:56 PM (3 years, 8 months ago)

Damnit.  The Agar I bought has sugar in it.

edit - fuck it - sugar content is 10g per serving.  Just going to go with it and see what happens.

Edited by Thorasta (10/01/20 06:07 PM)

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Offlineadhoc
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Re: Pastywhyte's Easy Agar Tek [Re: Pastywhyte]
    #26998399 - 10/22/20 05:47 PM (3 years, 8 months ago)

Quote:

Pastywhyte said:
Store blank plates at room temp. Never refrigerate them, save that for when you have something growing on it that you want to stall.




FWIW the tek write up says

Quote:

Pastywhyte said:
Then I when remove them they go straight into my SAB. If you want to keep em around for a while they can be stored safely in the fridge, just don't unwrap em.



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Offlinetryptkaloids
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Registered: 02/08/15
Posts: 12,698
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Re: Pastywhyte's Easy Agar Tek [Re: adhoc]
    #26998416 - 10/22/20 05:56 PM (3 years, 8 months ago)

The tek is also 6+ years old. Cultivators change as well as their habits and processes


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 

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Offlinelord_nikon6983
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Re: Pastywhyte's Easy Agar Tek [Re: tryptkaloids]
    #27000916 - 10/24/20 08:02 AM (3 years, 8 months ago)

I have had failure after failure here and im not sure whats going on. I am thinking maybe im overdoing the Karo.. would that cause nothing to grow on the plates?

I have made 3 batches of plates now. The first 2 batches i left them in the PC overnight which cause massive condensation. Also the syringes I had were 3 years old and did not seem to have many spores in the compared to this new order i got.

The first 2 rounds have been sitting for over 3 weeks now with NO growth. I figured either the spores were bunk or there was too much condensation.. literally if I tip them sideways a good amount of water collects on the side.

After getting new spores I read some different no poor teks and did NOT leave them overnight OR use micropore tape - basically did ziran style with twist top ziploc containers but still followed this recipe.  I knocked up 6 plates, 3 each of 2 dif strains. It has been 3 days now, sitting at 75 degrees and I see nothing.

As I said, after re-reading some stuff maybe i over did the karo? It was more of a dollap. Is it possible that there is too much karo in there? I dont know what else could be wrong - I got rid of the condensation problem and got new spores.


--------------------
Not all those who wander are lost

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InvisiblePastywhyteMDiscord
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Registered: 09/15/12
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Re: Pastywhyte's Easy Agar Tek [Re: lord_nikon6983]
    #27000929 - 10/24/20 08:13 AM (3 years, 8 months ago)

3 days isn’t much time for spores to germinate. Maybe if the spores were super fresh you would see 3 day germination. If you don’t see anything in 3 weeks then you can be concerned. Even then it’s far more likely the spores were just old rather than the karo causing the issue. Karo won’t prevent the germination of spores, it’s a non issue.

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OfflineFunnelMind
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Registered: 09/22/20
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Re: Pastywhyte's Easy Agar Tek [Re: Pastywhyte]
    #27001816 - 10/24/20 07:50 PM (3 years, 8 months ago)

Hi, I am new here and love this idea as a TEk. I started doing it tonight, and I am sorry if this has already been answered, but I was confused when you had said you use 2 heaping tablespoons. Did you mean agar? I saw you said to use 2.5 grams, and I am using MEA agar. I put in 1 teaspoon of MEA agar and half a cup of distilled water. It is in the pressure cooker now, but I just weighed 1 teaspoon of my MEA agar on my digital scale, and it says it is 4 grams. Did I add too much MEA agar? I won't be opening my PC until tomorrow, when I have my SAB setup and cleaned. Also, i watched the videos you linked to, and saw that it said to use 2.5 grams of agar. Would you mind clarifying how much MEA agar I should use with half a cup of water? I am making 8 mini glad rounds of agar to inoculate with spore prints. Thank you for all the great information!


--------------------
Thanks,
FunnelMind

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Offlinelord_nikon6983
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Re: Pastywhyte's Easy Agar Tek [Re: FunnelMind]
    #27005843 - 10/27/20 07:53 AM (3 years, 7 months ago)

Quote:

Pastywhyte said:
3 days isn’t much time for spores to germinate. Maybe if the spores were super fresh you would see 3 day germination. If you don’t see anything in 3 weeks then you can be concerned. Even then it’s far more likely the spores were just old rather than the karo causing the issue. Karo won’t prevent the germination of spores, it’s a non issue.




Thanks for the reply pasty! I guess I read on some agar tek (realizing now it wasnt this one) that you can start checking plates after 3 days.

my original 2 rounds of plates have now been sitting for 24 days with no growth. I DID take them out of the my incubator when I made this new round with the new spores, so they were in a steady 73-75 degrees for 18 days and now are sitting out in variable temperature from probably 58-80 degrees for the other 6 days.


My second round of plates (screw top without any FAE holes) has been in the incubator @ 73-75 degrees for 7 days now, i dont see anything. I THINK i can see a little black clump of spores on 2 of them...but nothing white at all.


I guess I thought the whole reason i was trying agar was that you get pretty quick results...

Is it possible im doing something wrong with the inoculation? I couldnt seem to find much guidance for that part. I just opened the container as little as possible, flamed the needle, let a few drop hiss out and then put a few drops on the plate.. Do i need to stick the needle down in to the agar? Do I need to disturb the agar and put the drop from the syringe so that the spores actually make contact with the agar?

Thanks for your time


--------------------
Not all those who wander are lost

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InvisiblePastywhyteMDiscord
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Re: Pastywhyte's Easy Agar Tek [Re: lord_nikon6983]
    #27005883 - 10/27/20 08:34 AM (3 years, 7 months ago)

Agar isn’t about quick results, agar is about consistency and having control over your cultures. Shortcuts are often time wasters in this hobby, the long way is what delivers good results.

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Offlinelord_nikon6983
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Re: Pastywhyte's Easy Agar Tek [Re: Pastywhyte]
    #27008109 - 10/28/20 10:59 AM (3 years, 7 months ago)

any guidance on the inoculation? I remember in biology class we used the loops to scratch the surface of the agar. I just feel like even though I managed to get the condensation levels down somewhat, there is still a film of water across the whole plate, the spores are probably just sitting in the liquid and never touching the nutrients.


--------------------
Not all those who wander are lost

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Offlinecheesemaster
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Registered: 12/21/17
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Re: Pastywhyte's Easy Agar Tek [Re: lord_nikon6983]
    #27009604 - 10/29/20 03:51 AM (3 years, 7 months ago)

Thanks for the tek, been a huge help to me getting started with agar. Im a few transfers deep and things are starting to look good.


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OfflineFunnelMind
Student
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Registered: 09/22/20
Posts: 14
Last seen: 2 years, 6 months
Re: Pastywhyte's Easy Agar Tek [Re: Pastywhyte]
    #27015695 - 11/01/20 12:44 PM (3 years, 7 months ago)

Despite kind of messing up the agar. I am not sure if I understood the measurements right, I used 1 teaspoon of MEA and half a cup of distilled water, I am having some germination on all my spores! When I weighed 1 teaspoon of powdered MEA agar on a scale, it weighed 4 grams. I still fill like the agar turned out a little more runny than I would have liked. I just wanted to note, that next time I think I will try 1 and 1/2 teaspoons of powdered agar. I will post here again to let people know how it goes, if no one can clarify the measurements for me (for making agar for 8 mini glad rounds). Anyways, wanted to say this tek is working for me and I don't think I even did everything 100% correctly. Looking forward to the next 2 weeks.


--------------------
Thanks,
FunnelMind

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Invisiblemagicman11
CuriousNoob
Registered: 09/29/20
Posts: 3
Re: Pastywhyte's Easy Agar Tek [Re: FunnelMind]
    #27016543 - 11/01/20 10:37 PM (3 years, 7 months ago)

Hey Pasty, (everyone)

I'm new here, and made my 1st batch of agar 2 week ago thanks to you and Munch. I used Munch's agar recipe since I was only going to make 10 jars. It was super EZ!! Hardest part was watching psi on PC. lol

I had a bit of condensation after PC that was not re-absorbed by the agar. Will this affect myc growth? Can the agar surface have water on it (be wet) or should it be dry and shinny like a petri?






I inoculated last week, but before inoculating, I flipped my jar upside down to remove the excess water in my SAB. I flamed my syringe and placed 2 drops in jars, but all I got was mold. No myc growth at all!!

Would the wet agar surface or flipping jar upside down be enough to get contams and stop myc growth? Jar was only open about 10 sec's. If that.

Also, not that it matters, but I made PDYA instead of PDA by accident. Wasn't paying attention and added yeast like a dumbass. I doubt this had any impact on myc growth.

Thanks for keeping it simple!! :thumbup:

Edited by magicman11 (11/01/20 10:46 PM)

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Invisiblemagicman11
CuriousNoob
Registered: 09/29/20
Posts: 3
Re: Pastywhyte's Easy Agar Tek [Re: Pastywhyte]
    #27016559 - 11/01/20 11:02 PM (3 years, 7 months ago)

Quote:

Pastywhyte said:
3 days isn’t much time for spores to germinate. Maybe if the spores were super fresh you would see 3 day germination. If you don’t see anything in 3 weeks then you can be concerned. Even then it’s far more likely the spores were just old rather than the karo causing the issue. Karo won’t prevent the germination of spores, it’s a non issue.





Hey Pasty,

Having similar issues with my inoculation.

I got some growth 3 days in. Now at day 6, I can see its mold. There is no myc growth. I was about to trash them but just read your post - "myc can take up to 3+ weeks to grow before being concerned".

So do I keep the jars and wait for myc growth or trash em? 








--------------------
Free your mind!!

Edited by magicman11 (11/01/20 11:50 PM)

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OfflinePsyche delics
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Re: Pastywhyte's Easy Agar Tek [Re: magicman11]
    #27017115 - 11/02/20 09:20 AM (3 years, 7 months ago)

Where did you get the syringes from I had a similar problem when I first started growing from a contaminated syringe. I would just throw those out mold can grow pretty quickly if you're not seeing myc growth by time contams take hold theyll most likely take over the plate ime.


--------------------

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InvisiblePastywhyteMDiscord
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Re: Pastywhyte's Easy Agar Tek [Re: Psyche delics] * 1
    #27017117 - 11/02/20 09:21 AM (3 years, 7 months ago)

If you’re seeing mold that quick there is nothing to save. I would source new spores and start over.

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OfflineFunnelMind
Student
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Registered: 09/22/20
Posts: 14
Last seen: 2 years, 6 months
Re: Pastywhyte's Easy Agar Tek [Re: Pastywhyte]
    #27023854 - 11/05/20 07:11 PM (3 years, 7 months ago)

So, I used your TEK, and I have 3 different types of mushrooms that I inoculated via spore prints, in an SAB box. This one has the most growth.

I have grown mushrooms in the past, but this is my first attempt at agar. I was concerned at first that I might have cobweb mold, since it is so hard to see the growth in the agar and I didn't use food colouring. After seeing some other posts, I believe this is mycelium, especially since all the glad rounds that I inoculated look the same way, and the little black spots in the middle of the growths are the groups of spores that I scraped off the spore print, into the agar, when I inoculated them. In all of my glad rounds the growth is growing outwards, away from the spores (black spots in pics).

It has been 11 days since I inoculated the agar. Do you think I just over did it with the spores, and that I have a bunch of competing mycelium? Do you think I can transfer to grain in a couple of days? Any thoughts or suggestions would be greatly appreciated.

Thank You!






Edited by FunnelMind (11/06/20 06:19 PM)

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Offlinewaxbrain
That Fun Guy
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Registered: 05/13/17
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Re: Pastywhyte's Easy Agar Tek [Re: FunnelMind]
    #27075220 - 12/06/20 12:35 PM (3 years, 6 months ago)

so.. how are these supplies? anyone use the super agar powder?



note: these are all old supplies i found in my basement from probably 3 and a half years ago

Edited by waxbrain (12/06/20 12:38 PM)

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OfflineNOMADIC
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Registered: 11/24/10
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Re: Pastywhyte's Easy Agar Tek [Re: waxbrain]
    #27077808 - 12/07/20 08:33 PM (3 years, 6 months ago)

Quote:

Inocuole said:
Quote:

Munchauzen said:
no physical contact.



And if you interact, your grow is on contract.





Best bet is to play it safe MF
So don’t F up yo plates

Edited by NOMADIC (12/07/20 08:35 PM)

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