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InvisibleMunchauzen
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Re: Pastywhyte's Easy Agar Tek [Re: MadHatR]
    #26896402 - 08/24/20 04:32 AM (3 years, 10 months ago)

Quote:

MadHatR said:
Quote:

tryptkaloids said:
Its not that it's not easier its that it's more risky.




How is it more risky? It's not that I doubt that you are correct, I just like to know the science behind it. Without knowing why something is happening deprives you improving and innovating. If we all just keep doing things a certain way for the soul reason that past experience has shown us that doing it that way works then we never advance. At one point we all thought you had to build a incubator to get grain to spawn. There was a time when Gloveboxes were considered a great option until someone pointed out that the negative air pressure caused by attaching gloves to the box actually pulled contaminated air into the box. If I didn't know the concept behind how a flow-hood works then I might think that taping a filter to a box fan and blowing it over your work area might help, sorry Willy.

I would think that by creating a sealed space inside the dish that the only thing that enters that space is a needle that was just flame sterilized would be a good thing. When you take the top off the dish then due to a change in air pressure air flow would be created in the SAB. The process of moving the lid out of the way to inject spores, I would think, would have a higher chance of contamination.

Sorry for the rant, but a few years ago I signed on, was reading up on all the Tek's and I floated the idea of taking half pint jars, pouring a layer of agar on the bottom, knock up, grow out, then inject water into the jar to break up mycelium that you could then suck back up to make LC syringes. I was told that if I wasn't confident enough to drop a wedge in a jar of LC solution then I shouldn't be working with agar. Fast forward a few years and I found out that not only was my idea not bad, but Pasty had already came up with a similar idea and was having good results with it.

I also don't understand why it is that we cut large wedges out for transfer instead of a needle biopsy. I know with a larger sample size, the mycelium should repair and start spreading faster than a small sample. This will give it a greater chance of fighting off any contamination, and of course move you toward the finish line faster. I have all the time in the world, and am in no rush because I know that I can time things so once you are finishing your last flush on your current batch your next batch is ready. The same concept that I used to use when growing green. In that case you would have 3 tents running all the time. Tent 1 would be in veg for 30 days, tent 2 would be on the first 30 days of bloom and the 3rd tent would be on the last 30 days of bloom. That allowed you to rotate the cycles around so that your were always harvesting.




Your method requires the sterile needle to touch a sanitized SHIP. Physical contact to a non-sterile object is a much larger vector than using proper technique to remove the lid. Plus introducing water encourages contaminate germination. A 3-month lifespan for a monotub is not common. They typically last a month or so start to finish.

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OfflineMadHatR
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Re: Pastywhyte's Easy Agar Tek [Re: Munchauzen]
    #26896469 - 08/24/20 06:29 AM (3 years, 10 months ago)

Quote:

Munchauzen said:

Well if you have a disability or special needs, by all means do whatever you see fit for your circumstances. But I would find a method besides liquid biopsy syringes. Thats asking for trouble. Introducing water at every transfer is a pretty big vector. SHIPs are also a pretty big vector as well. maybe a tiny metal scoop of some kind to make transfers in a single movement? also, get some pop top lids instead of screw ons. they open with just a slight squeeze on the sides, maybe those would be easier to handle?




When I became disabled going on 6 years ago, I have gaps in my cognitive abilities and lost control in some motor skills. Modern medicine has only really succeeded in making my brain dependent on benzo's and other medication with nasty side effects.

Since returning to my old career would be challenging and big pharma only seems to care about making money instead of making me better. I decided to move to a laid back city. The result being I am surrounded by the largest cluster of Botanical Tea Bars. Kava, Kratom and Blue Lotus has done more for me in the last year than 5 years of taking pills. This lead me to return to my passion of mycology. Mushrooms like Lion's Mane have been shown to rebuild brain cells and improve cognition. As far as I can tell there are no local cultivators of medicinal mushrooms.

Long story short...to late for that. I have been watching lots of videos and doing lots of reading about how to start a small business growing gourmet mushrooms. I have been trying to incorporate some of the techniques that the Professional Mushroom Growers and Mycology Scientist use, but of course on a budget. They make things like doing a culture biopsy from the center of a mushroom to make a clone look easy. Of course they are preforming advanced techniques in a lab or other such controlled environment.

I'm sure in time I will, through trial and error, put my own spin on it that works best for me and others with difficulties.

I also forgot how long in the beginning things can take. It's tons of rush, rush, rush, wait, wait, wait. That leaves much free time to ponder if there is a better way.


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Never Judge a man until you walk a mile in his shoes, because then you are a mile away and you have his shoes.

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OfflineA.k.aM
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Re: Pastywhyte's Easy Agar Tek [Re: MadHatR]
    #26896596 - 08/24/20 08:38 AM (3 years, 10 months ago)

Look up the josex poke method.

Should give you flashbacks to the agar in a jar lc thing lol.


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LAGM2020

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Offlinetryptkaloids
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Re: Pastywhyte's Easy Agar Tek [Re: A.k.a]
    #26896887 - 08/24/20 11:08 AM (3 years, 10 months ago)

Using an inoculation loop for small, quick transfers has always been superior IME considering you don't need to pc it like a syringe of water.


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 

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OfflineMadHatR
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Re: Pastywhyte's Easy Agar Tek [Re: Munchauzen]
    #26898063 - 08/25/20 03:15 AM (3 years, 9 months ago)

Quote:

Munchauzen said:

Your method requires the sterile needle to touch a sanitized SHIP. Physical contact to a non-sterile object is a much larger vector than using proper technique to remove the lid. Plus introducing water encourages contaminate germination. A 3-month lifespan for a monotub is not common. They typically last a month or so start to finish.




That is very logical. Since we didn't have SHIPs when I was learning I never went through the debates on how well they work. When I started researching again I was very excited to see the use of injection ports and whatman filters. While many people maybe burned out on them, they are new to me.

When I started out LC was all the rage. If I recall right I did the PF Tek once then bought a PC and went straight to LI due to using most of my MS on the PF run. After seeing people's opinion on LI I guess I was just extra careful back in the day or very lucky because I had great success. I would at most have 1 grain jar out of 12 that looked sketchy. I also only had 1 shoebox tub out of more than I can count that contamed out. Looking back at all the methods that we thought were good and have now been proven to be wrong, I am surprised we got anything to grow. It's like looking back in history and thinking how crazy people were by thinking leaches removed toxins. I am sure in 100 years people will look back at us and laugh at all the things we got wrong.

I guess we are usually drawn to the methods that we learned on and had success with, at-least until we start having success with another method. It doesn't help that people like Home Myco make it look so simple while flouting the rules. I usually try and balance out one of his video's with video's by someone like Southwest Mushrooms or Mossy Creek, people that make their living growing on a massive scale.

As for the 3 month monotub, I wasn't referring to growing mushrooms, but something much bigger, stickier and greener. What I was trying to point out is that if you time things out right, then once things get rolling you will stay busy. As soon as you finish the last flush on one tub your supplies are ready to start a replacement tub.


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Never Judge a man until you walk a mile in his shoes, because then you are a mile away and you have his shoes.

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OfflineMadHatR
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Re: Pastywhyte's Easy Agar Tek [Re: A.k.a]
    #26898066 - 08/25/20 03:23 AM (3 years, 9 months ago)

Quote:

A.k.a said:
Look up the josex poke method.

Should give you flashbacks to the agar in a jar lc thing lol.




Man it seems every idea I come up with, someone else has thought of it first and probably tried it. There are so many huge threads here that you can spend days just going through the hundreds if not thousands of pages of reply's. That is not even including when that thread leads you to another and so on.


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Never Judge a man until you walk a mile in his shoes, because then you are a mile away and you have his shoes.

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Offline42wallabyway
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Re: Pasty Agar Tek [Re: MadHatR]
    #26908350 - 08/30/20 04:45 PM (3 years, 9 months ago)

I have tried this method three times and each time I end up with a layer of water on top of my agar inside the jars after sterilizing them. First I thought I had too much water so I tried a steaming basket, but still no luck. I don’t know what I’m doing wrong but the extra moisture ruins the PDA and I have to start from scratch. Any suggestions would be extremely appreciated!

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Offlinetryptkaloids
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Re: Pasty Agar Tek [Re: 42wallabyway]
    #26908360 - 08/30/20 04:52 PM (3 years, 9 months ago)

Let them cool and dry before putting the lids on.


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 

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Offlinekrunkmaster
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Re: Pasty Agar Tek [Re: tryptkaloids]
    #26908509 - 08/30/20 06:06 PM (3 years, 9 months ago)

Been out of the game for about ~7 years and wanted to get back into it and see what new techniques people had come up with. I prepared these last night and was pleased at how easy it was :thumbup: thanks so much Pasty!

I tried doing MS syringe inoculation today. We'll see how it goes... I don't feel confident in my technique. I had worked with agar once before but was cloning from a fruit. I struggled this time with getting the syringe to behave... it seemed to have two settings: "off" and "shotgun blast" :stars:

I was flame sterilizing between each plate then squirting a little jet each time before inoculating, but even after the squirt, it was super hard to control the plunger enough to get a little drop out. I don't know if it was my own technique or maybe the syringes I was using (I only ever did straight MS -> grain before so never needed this much control).

Any thoughts? I have more syringes, so I'm down to try again!


--------------------
Thou shalt not kill my vibe

Edited by krunkmaster (08/30/20 06:07 PM)

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OfflineDoc9151M
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Re: Pasty Agar Tek [Re: krunkmaster]
    #26913904 - 09/02/20 02:55 PM (3 years, 9 months ago)

I don't use syringes for anything but PF tek,  even then I prefer agar.


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Psilocybe cubensis data collection thread. please help with this project if you hunt wild cubensis.
https://www.shroomery.org/forums/showflat.php?Cat=0&Number=26513593&page=0&vc=1#26513593

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Re: Pasty Agar Tek [Re: Doc9151]
    #26915585 - 09/03/20 12:26 PM (3 years, 9 months ago)

I definitely want to move away from syringes once I am established and able to self-sustain, but with no fruits to clone and no spore prints left over I had to start from somewhere :tongue2: I was eager to start working with agar and had the syringes on hand so I wanted to give it a try.

I did however decide not to bank 100% on this to get up and running, so I also did a spore syringe inoculation straight to grain yesterday. If I can get some fruits out of that then I'll start cloning to agar and taking spore prints from there :biggrin:


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Thou shalt not kill my vibe

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Re: Pastywhyte's Easy Agar Tek [Re: Pastywhyte]
    #26929909 - 09/11/20 12:23 PM (3 years, 9 months ago)

Can anyone tell me a reliable place to buy micropore tape that is not online? I went to CVS but they only had transpore, which i have discovered will melt in PC.


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Not all those who wander are lost

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Offlinetryptkaloids
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Re: Pastywhyte's Easy Agar Tek [Re: lord_nikon6983]
    #26930018 - 09/11/20 01:29 PM (3 years, 9 months ago)

Rite aid or walmart


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 

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OfflinePsyche delics
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Re: Pastywhyte's Easy Agar Tek [Re: Munchauzen]
    #26930026 - 09/11/20 01:36 PM (3 years, 9 months ago)

Quote:

Munchauzen said:
A 3-month lifespan for a monotub is not common. They typically last a month or so start to finish.




I just finished a shoebox that lasted  4 months. It was crazy.


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Edited by Psyche delics (09/11/20 01:36 PM)

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Re: Pastywhyte's Easy Agar Tek [Re: Psyche delics]
    #26930040 - 09/11/20 01:47 PM (3 years, 9 months ago)

Like kept giving fruits?

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OfflinePsyche delics
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Re: Pastywhyte's Easy Agar Tek [Re: InnerWisdom]
    #26931526 - 09/12/20 10:16 AM (3 years, 9 months ago)

Ya and not bad flushes either.


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Edited by Psyche delics (09/12/20 10:18 AM)

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Re: Pastywhyte's Easy Agar Tek [Re: Psyche delics]
    #26931724 - 09/12/20 12:10 PM (3 years, 9 months ago)

substrate?

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Re: Pastywhyte's Easy Agar Tek [Re: InnerWisdom]
    #26931802 - 09/12/20 01:07 PM (3 years, 9 months ago)

Wbs->coco coir


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Offlineraoulduke69
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Re: Pastywhyte's Easy Agar Tek [Re: Pastywhyte]
    #26932521 - 09/12/20 09:22 PM (3 years, 9 months ago)

How long could you leave the containers in the PC for? If you're not going to inoculate them for another 24 hours or longer, is it better to leave them in the SAB or store them in the fridge?

If you store them in the fridge, how long do they need to sit in the SAB before you inoculate them?

I'm a noob,if you can't tell.

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OfflinePsyche delics
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Re: Pastywhyte's Easy Agar Tek [Re: raoulduke69]
    #26932904 - 09/13/20 06:31 AM (3 years, 9 months ago)

They're fine to leave room temp for 24 hours or even a few days or more. If you put them in the fridge you can inoculate them immediately after taking them out.


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