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InvisibleMudaFuka
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Re: Pastywhyte's Easy Agar Tek [Re: Imperfect Iam]
    #20470244 - 08/24/14 02:11 PM (9 years, 10 months ago)

I used to use 2 drops of honey. Now I use 7 grams of dextrose per 500ml of water.


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Offlinepawnzy
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Re: Pastywhyte's Easy Agar Tek [Re: MudaFuka]
    #20470366 - 08/24/14 02:41 PM (9 years, 10 months ago)

Quote:

MudaFuka said:
I used to use 2 drops of honey. Now I use 7 grams of dextrose per 500ml of water.



ok so 3.5 per 250 or 1.7 per 120.. nice

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Re: Pastywhyte's Easy Agar Tek [Re: pawnzy]
    #20471213 - 08/24/14 06:22 PM (9 years, 10 months ago)

I sterilised 12 tubs and only used 6.  Theyve been in the PC for 2 weeks.  Are they toast or can i re sterilise

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Re: Pastywhyte's Easy Agar Tek [Re: Edmunter]
    #20471244 - 08/24/14 06:29 PM (9 years, 10 months ago)

Use em, they are fine :thumbup:

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InvisibleImperfect Iam
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Re: Pastywhyte's Easy Agar Tek [Re: MudaFuka]
    #20471456 - 08/24/14 07:08 PM (9 years, 10 months ago)

Quote:

MudaFuka said:
I used to use 2 drops of honey. Now I use 7 grams of dextrose per 500ml of water.





I use 20 grams agar

20 grams LME

2 grams yeast

1000 ml. of water

and a drop of karo, so I guess two drops was right on anyway, I'm so forgetful

I saved some WBS soak water(gotta get to pc'ing some WBS tonight), I forget the numbers on that, cause it's in ml
I think, and it's been some time since I made it, damn short term memory!


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All you touch, and all you see, is all your life will ever be- Pink Floyd

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InvisibleMudaFuka
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Re: Pastywhyte's Easy Agar Tek [Re: Imperfect Iam]
    #20471477 - 08/24/14 07:10 PM (9 years, 10 months ago)

I haven't had much success with grain water agar.


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InvisibleImperfect Iam
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Re: Pastywhyte's Easy Agar Tek [Re: MudaFuka]
    #20471553 - 08/24/14 07:24 PM (9 years, 10 months ago)

Really, I don't recall having any problems, except the consistency was to watery, so Violets recommended amount of grain water must be a little high, or the amount of agar low, unless I fucked it up somehow, but it has came out thin consistency everytime, I think I have only done it twice thought.


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All you touch, and all you see, is all your life will ever be- Pink Floyd

Life is what happens while you're busy making other plans- John Lennon

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InvisiblebodhisattaMDiscordReddit
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Re: Pastywhyte's Easy Agar Tek [Re: Imperfect Iam]
    #20471573 - 08/24/14 07:27 PM (9 years, 10 months ago)

consistency is based on gel strength, you should always use about 9/10g to 500ml or 18/20g to a liter. that will get you the gel strength you need, I always wondered about consistency in nutrient strength though.

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Re: Pastywhyte's Easy Agar Tek [Re: bodhisatta]
    #20471595 - 08/24/14 07:30 PM (9 years, 10 months ago)

I always bang a little extra agar when doing grain water agar cause I find its thin as well. Cornstarch agar on the other hand I use more like 16-17 grams agar to 1L cause that shit makes your agar stiff as hell. How hard your agar ends up depends on a few factors that everyone should learn and keep in mind.

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InvisibleMudaFuka
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Re: Pastywhyte's Easy Agar Tek [Re: Pastywhyte]
    #20471695 - 08/24/14 07:47 PM (9 years, 10 months ago)

Consistency was my problem. The first batch I tried came out like pudding. I find potato flake and dextrose works really well for me so I haven't bothered trying grain water again in a while.


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Offlinetombosley8
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Re: Pastywhyte's Easy Agar Tek [Re: MudaFuka]
    #20472010 - 08/24/14 08:55 PM (9 years, 10 months ago)

why do my spores from ms syringes take so long to germinate(5-6 days) on agar and only a few of the plates germinate at all. I'm using only ELME and agar agar. Once mycelium is live everything is normal but germinating spores, no.  I'm only using one drop (some 2). I can germinate in LC or grain fine but then i transfer that to agar so it's a step backwards. Anyone else? or is this normal? I've used syringes from two different sources. How much solution do you dump out to cool needle before inoculating? I wait til i see two drops after the sizzle. Enough? Maybe i need to just make my own prints but i'm lazy (so i just bought them... well syringes actually). I just want to see if i can get more variety and better genetics from ms than clones.


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Edited by tombosley8 (08/24/14 08:58 PM)

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InvisiblebodhisattaMDiscordReddit
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Re: Pastywhyte's Easy Agar Tek [Re: tombosley8]
    #20472053 - 08/24/14 09:03 PM (9 years, 10 months ago)

I've only ever used ELMEA LMEA or MEA and spores do have a hard time germinating on it for whatever reason. I do like to flame, squirt out some into a dish or paper towel till i know the needle is cool, then i squirt it onto a makeshift loop then hit the agar with that, seems to give better results but spores to agar has always been a hit or miss thing for me.

Edited by Trusted cuItivator (08/24/14 09:12 PM)

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Offlinetombosley8
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Re: Pastywhyte's Easy Agar Tek [Re: bodhisatta]
    #20472083 - 08/24/14 09:08 PM (9 years, 10 months ago)

good to know i shouldn't be complaining anyways it's only a drop and all the capability that one drop has. Can't believe i ever noc'd grain up with MS. Have to say for some reason my pasty plates had better over all success with spore germination(:shrug:)


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InvisibleViolet
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Re: Pastywhyte's Easy Agar Tek [Re: Imperfect Iam]
    #20472154 - 08/24/14 09:22 PM (9 years, 10 months ago)

Quote:

MudaFuka said:
I saved some WBS soak water



I wouldn't be certain of ideal nutrition in grain water from anything other than grass seed or brown rice.
All the other grains leave the water too pale and, I believe, low on nutrition unless reduced a lot.

If you intend to use grainwater, start with grass seed or brown rice.  IME those are by far the best grains anyway, for a myriad of reasons.


Quote:

Imperfect Iam said:
Really, I don't recall having any problems, except the consistency was to watery, so Violets recommended amount of grain water must be a little high, or the amount of agar low, unless I fucked it up somehow, but it has came out thin consistency everytime, I think I have only done it twice thought.



I don't see how that could happen unless you're not doing something right.  It doesn't matter what nutrient is suspended in the liquid - the proper proportion of agar should gel it up just right, regardless.

Doesn't matter what the nutrient is:
9-10g agar-agar powder per 500mL solution, or .5g agar-agar powder per 50mL solution (NOT an agar/malt mix etc.)


Imperfect Iam:  Are you doing no-pour agar containers?  Talking about "my" recommended "amount of grain water" suggests you are.
If so, maybe *just maybe* you're using agar that has to be boiled before you load it for sterilization.  Some agar types won't gel unless they're boiled first.  Dunno, just a thought.


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InvisiblePastywhyteMDiscord
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Re: Pastywhyte's Easy Agar Tek [Re: Violet]
    #20472353 - 08/24/14 10:03 PM (9 years, 10 months ago)

Quote:

Violet said:
I wouldn't be certain of ideal nutrition in grain water from anything other than grass seed or brown rice.
All the other grains leave the water too pale and, I believe, low on nutrition unless reduced a lot.




I don't agree. I have used wheat, rye, millet and oat grain soak water and they all performed equally well to the RGS agar soak water I tried as well.

Quote:

Violet said:
If so, maybe *just maybe* you're using agar that has to be boiled before you load it for sterilization.  Some agar types won't gel unless they're boiled first.  Dunno, just a thought.




I have experienced this myself, perhaps I should add a note to the OP.

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Re: Pastywhyte's Easy Agar Tek [Re: Pastywhyte]
    #20472447 - 08/24/14 10:19 PM (9 years, 10 months ago)

Quote:

Pastywhyte said:
Quote:

Violet said:
I wouldn't be certain of ideal nutrition in grain water from anything other than grass seed or brown rice.
All the other grains leave the water too pale and, I believe, low on nutrition unless reduced a lot.




I don't agree. I have used wheat, rye, millet and oat grain soak water and they all performed equally well to the RGS agar soak water I tried as well.



You don't agree with my uncertainty?  hehehe


This much is a fact, at least:
Grass seed and brown rice leave MUCH more of the nutritious starch in the water (which is settled to pour off "clear" grainwater for agar use) and the water they leave behind is so much thicker with nutrition that it can be diluted 2x, even 3x, before it is as dense as grainwater from other grains.

So I wouldn't be at all surprised if grainwater from ANY grain works, because they all leave behind the same goodies the grains have that mycelium loves.
But when I've had containers full of gs/br grainwater next to water from birdseed, the birdseed water has never been worth keeping.


--------------------
Intentionally or not, here in mushcult we are purveyors of love culture and enlightenment movement. Let's try to act like it!

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The simplest, quickest, safest tek!  For beginners, culturers, lazy people, stealth lovers, contam haters, and alternative seekers!
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Offlineaykaye47
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Re: Pastywhyte's Easy Agar Tek [Re: Violet]
    #20473134 - 08/25/14 01:50 AM (9 years, 10 months ago)

Have you guys ever takin a piss test well I got a bunch of pre sterilized urine sample bottles do you think I can use these instead of plates ? And do you think I should just pour from a separate bottle or use the pasty plate method they are pp5 and if I use pasty method should I put a hole in the cap with a filter or just leave cap unscrewed covered with foil? This a pic of a used bottle I use for my dank


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Re: Pastywhyte's Easy Agar Tek [Re: aykaye47]
    #20473136 - 08/25/14 01:53 AM (9 years, 10 months ago)

There hard to maneuver in


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Offlineaykaye47
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Re: Pastywhyte's Easy Agar Tek [Re: cronicr]
    #20473144 - 08/25/14 01:59 AM (9 years, 10 months ago)

What else could I use them for? I got like a big box full probably about 500 of them I was thinking I could use them instead if plates would that all I would have to worry about ? Maneuverability ?


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Re: Pastywhyte's Easy Agar Tek [Re: aykaye47]
    #20473151 - 08/25/14 02:04 AM (9 years, 10 months ago)

Just get creative and use something other then a scalpel, like a screwdriver but those would really only be good for cleaning cultures, isolating would be a bitch lol


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