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Offlinebollarice
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Registered: 03/19/14
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Re: Pastywhyte's [Re: bollarice]
    #20262691 - 07/12/14 12:16 PM (9 years, 11 months ago)

so, once you've transferred a wedge into a new plate, do you toss the old agar? part of me wants to bury under some coir and poo and see what goes down.


--------------------


I've been workin' in the Kremlin with a two-headed dog.

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Offlinetripdawg420
low life with no life
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Registered: 02/02/09
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Re: Pastywhyte's [Re: bollarice]
    #20262701 - 07/12/14 12:19 PM (9 years, 11 months ago)

ya i take a few transfers then toss it


--------------------
HUSTLER
How U Survive This Life Everyday Resourcefully
epic GT mono tub
http://www.shroomery.org/forums/showflat.php/Number/17277772

wbs tek
http://www.shroomery.org/forums/showflat.php/Number/11525679
coir tek
http://www.shroomery.org/forums/showflat.php/Number/11917410
results :thumbup:

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Invisibleblindingleaf
blue collar underworld
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Registered: 07/19/13
Posts: 22,008
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Re: Pastywhyte's [Re: tripdawg420]
    #20262737 - 07/12/14 12:30 PM (9 years, 11 months ago)

i used to keep mine all the time, in case transfer went wrong or something.  then ended up with fridge/tote full of used plates.

if ur doing plate>qt>g2g u should save it tho, cause if its a great culture, u wanna be able to go back and use it again/slant it.
thats for clones and isolates tho.
if its just MS plates, then u can save or toss


--------------------
A few thoughts on cultivation
MICROBIAL HUSBANDRY!!!!

The whole is greater than the sum of its parts

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Offlinebollarice
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Re: Pastywhyte's [Re: blindingleaf]
    #20262764 - 07/12/14 12:39 PM (9 years, 11 months ago)

word. it was ms plates. burried it in the shade with some wet hpoo and coir for funsies.

when the first transfer spreads, I look for sectors I like, transfer those again and then when they are fully colonized, just dump the whole damn thing in a master, yeah?


--------------------


I've been workin' in the Kremlin with a two-headed dog.

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OfflineAttainment
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Registered: 04/10/14
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Re: Pastywhyte's [Re: bollarice]
    #20266814 - 07/13/14 01:07 PM (9 years, 11 months ago)

I picked up some agar agar from Whole Foods. It's in a powered form. Should I use less than the 2 tablespoons called for in the recipe. After adding 2 teaspoons of potato flakes and 1/2 cup water, the mixture is pretty thick. Any help would be appreciated.

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InvisiblePastywhyteMDiscord
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Re: Pastywhyte's [Re: Attainment]
    #20266830 - 07/13/14 01:13 PM (9 years, 11 months ago)

The 2 tablespoon ratio was for the ground bars. If I use powedered agar I have to weigh it, I prefer about 2-2.5 grams for 120ml of water. 2 tablespoons of powdered agar would be far too much, probably closer to 1 is what you would want.

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OfflineAttainment
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Re: Pastywhyte's [Re: Pastywhyte]
    #20266842 - 07/13/14 01:16 PM (9 years, 11 months ago)

yeah, I went ahead and added another 1/2 cup of water and 2 teaspoons of potato flakes and the consistency looks better. I'm guessing this stuff shouldn't be runny, but more 'mashed potato' like?

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InvisiblePastywhyteMDiscord
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Re: Pastywhyte's [Re: Attainment]
    #20266873 - 07/13/14 01:26 PM (9 years, 11 months ago)

After simmering it will be pretty smooth, like liquid jello. You don't want it too thick. It should probably be good now. If you have a scale and your using powdered agar, you want roughly 2% agar.

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OfflineAttainment
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Re: Pastywhyte's [Re: Attainment]
    #20266881 - 07/13/14 01:28 PM (9 years, 11 months ago)

nevermind, I see. 'stir until mixture is dissolved'

:facepalm3:

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OfflineAttainment
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Re: Pastywhyte's [Re: Attainment]
    #20266896 - 07/13/14 01:36 PM (9 years, 11 months ago)

thanks for replying. I swear I'm not gonna stop growing till I get one right. No matter how much I fuck up!

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InvisiblePastywhyteMDiscord
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Re: Pastywhyte's [Re: Attainment]
    #20266903 - 07/13/14 01:39 PM (9 years, 11 months ago)

Quote:

Attainment said:
thanks for replying. I swear I'm not gonna stop growing till I get one right. No matter how much I fuck up!




You will get it :thumbup: Persistence counts for a lot in this hobby :awesomenod:

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InvisibleMudaFuka
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Re: Pastywhyte's [Re: Pastywhyte]
    #20266969 - 07/13/14 02:00 PM (9 years, 11 months ago)

I use powdered agar from the health food store. I weigh mine out. 10 grams per 500ml of water, 7 grams of dextrose and 1 tablespoon of potato flake. I may start using more potato in the future so my cultures don't grow quite so fast. If I forget about a plate for a couple of days it gets completely over grown.


--------------------
AMU
Bottle Tek
Liquid Inoculant Tek                                   
                     

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OfflineAttainment
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Registered: 04/10/14
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Re: Pastywhyte's [Re: MudaFuka]
    #20270773 - 07/14/14 11:31 AM (9 years, 11 months ago)

Plates came out looking good. Used 1 tablespoon of the powdered agar. I believe RR says in his videos that 1 tablespoon = 2 grams.
My plan is to get good growth on them from a spore syringe I made last night and then use that to inoculate grain jars with.
that way I hopefully won't waste any Rye berries to a contamed syringe.

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Offlinetripdawg420
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Re: Pastywhyte's [Re: Attainment]
    #20270786 - 07/14/14 11:34 AM (9 years, 11 months ago)

its easier to go print to plate but :thumbup:


--------------------
HUSTLER
How U Survive This Life Everyday Resourcefully
epic GT mono tub
http://www.shroomery.org/forums/showflat.php/Number/17277772

wbs tek
http://www.shroomery.org/forums/showflat.php/Number/11525679
coir tek
http://www.shroomery.org/forums/showflat.php/Number/11917410
results :thumbup:

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OfflineEdmunter
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Re: Pastywhyte's [Re: Attainment]
    #20270799 - 07/14/14 11:37 AM (9 years, 11 months ago)

Quote:

Attainment said:
Plates came out looking good. Used 1 tablespoon of the powdered agar. I believe RR says in his videos that 1 tablespoon = 2 grams.
My plan is to get good growth on them from a spore syringe I made last night and then use that to inoculate grain jars with.
that way I hopefully won't waste any Rye berries to a contamed syringe.




Im pretty sure its 6 grams.  Think about a gram of coke?

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OfflineAttainment
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Re: Pastywhyte's [Re: tripdawg420]
    #20270810 - 07/14/14 11:40 AM (9 years, 11 months ago)

u mean to just scrape the spores onto the plate? I thought about doing that, but read somewhere that using a syringe would be better. I should've just posted a question in the AMU thread beforehand

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InvisibleMrGiraffe

Registered: 04/04/14
Posts: 3,149
Re: Pastywhyte's [Re: Edmunter]
    #20270812 - 07/14/14 11:40 AM (9 years, 11 months ago)

Quote:

Edmunter said:
Quote:

Attainment said:
Plates came out looking good. Used 1 tablespoon of the powdered agar. I believe RR says in his videos that 1 tablespoon = 2 grams.
My plan is to get good growth on them from a spore syringe I made last night and then use that to inoculate grain jars with.
that way I hopefully won't waste any Rye berries to a contamed syringe.




Im pretty sure its 6 grams.  Think about a gram of coke?




All powders have a different density.  I usually use more spoonfuls of agar than I do elme for equal weights.

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OfflineAttainment
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Re: Pastywhyte's [Re: Attainment]
    #20270822 - 07/14/14 11:42 AM (9 years, 11 months ago)

@ edmunter. you're right. it's 6grams. I'm gonna go check my scale

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OfflineAttainment
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Registered: 04/10/14
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Re: Pastywhyte's [Re: Attainment]
    #20270833 - 07/14/14 11:44 AM (9 years, 11 months ago)

do you guys have to get your malt extract from online? there's no brewery's around me. just a winery

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OfflineEdmunter
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Re: Pastywhyte's [Re: MrGiraffe]
    #20270854 - 07/14/14 11:47 AM (9 years, 11 months ago)

Quote:

MrGiraffe said:
Quote:

Edmunter said:
Quote:

Attainment said:
Plates came out looking good. Used 1 tablespoon of the powdered agar. I believe RR says in his videos that 1 tablespoon = 2 grams.
My plan is to get good growth on them from a spore syringe I made last night and then use that to inoculate grain jars with.
that way I hopefully won't waste any Rye berries to a contamed syringe.




Im pretty sure its 6 grams.  Think about a gram of coke?




All powders have a different density.  I usually use more spoonfuls of agar than I do elme for equal weights.




Sure but thinking about a gram of coke on a Monday is decadent.

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Shop: Kraken Kratom Kratom Capsules for Sale   North Spore Injection Grain Bag   MagicBag.co All-In-One Bags That Don't Suck   Left Coast Kratom Buy Kratom Extract   Original Sensible Seeds Bulk Cannabis Seeds   Myyco.com Golden Teacher Liquid Culture For Sale   Bridgetown Botanicals Bridgetown Botanicals   Mushroom-Hut Liquid Cultures   OlympusMyco.com Olympus Myco Bulk Substrate   PhytoExtractum Kratom Powder for Sale


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