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elasticaltiger
Like Tigers in Coitus




Registered: 06/24/13
Posts: 8,438
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Re: Pasty Agar Tek [Re: mos619]
#20096933 - 06/07/14 04:29 PM (10 years, 24 days ago) |
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mos619 said: Started two of these with MS the other night, and I had a hard time getting the the lids one handed. I'm new to this, but I think screw offs might be easier to work with.
Also I think I'm going to look for something with a transparent lid like Happy Littletree found for my next batch since it's kinda hard to see what's going on in there from the edges.
Yeah you gotta crack them with two hands first.
Wipe the round down with iso, then your finger tips. Crack the lid. Then re wipe your finger tips if you feel like you need to. Proceed with inoculation.
Just a little bit different technique. I like that the lids stay put when they're all the way on.
Seems like they do kinda get loose after a while though. Like after 5 or 6 PC runs they don't stay on with the same 'grip' they did when they were new.
-------------------- First time growing cakes? DON'T make a Shotgun Fruiting Chamber The Shmuvbox. - The Old TC's Like it Afraid to Start Growing From Your Own Prints? Drop it Like a Tiger! No Pouring. No Syringes. No Cutting. No flaming. No Contamination. No Bullshit. "The best thing to do while your waiting is to start more stuff. I usually got so much happening that I have tossed projects simply because I didn't have time for them. -Pastywhite QFT Pastywhite's Easy Agar Tek (PastyPlates) Tiger Drop Video Demos By munchauzen Van Gogh would’ve sold more than one painting if he’d put tigers in them.―Bill Watterson EZEKIEL 23:20
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blindingleaf
blue collar underworld



Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
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Quote:
Like after 5 or 6 runs they don't stay on with the same 'grip' they did when they were new.
the condoms i use have the same problem
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,887
Loc: Canada
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Quote:
elasticaltiger said: Seems like they do kinda get loose after a while though. Like after 5 or 6 PC runs they don't stay on with the same 'grip' they did when they were new.
I have never really had much issue with this tho. One time I accidentally bumped the container pretty hard and popped the lid off but, now when they start to get a bit loose I just put em in ziplock bags. This helps to keep em from drying out if you wanna keep a plate kicking around for more than a few months (especially in the fridge).
I now have plates that have easily been through 12 runs. They are starting to get a little scuffed up on the sides and I am considering retiring them. But 12 runs x 8 plates is 96 plates. For $4 I consider that pretty decent value, especially since I never needed to order anything (when you live in Canada shipping is never free) and always have them on hand when I need em 
Been doing pour agar more often (in fact I plan to pour a few sleeves next week) cause of the fact that I am too cheap to buy gentamiacin and tetra is easy to pick up at the pet store. So pour agar is kinda required at that point. But I still use my pp5 plates for so many things, spore germination and cloning especially.
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MrGiraffe

Registered: 04/04/14
Posts: 3,149
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Just did my first batch last night. Still waiting on my agar to arrive in the mail, so I bit the bullet and paid $9 for an oz of the food flakes from the only place in town that had it. I had a stack of mini glads staring me down, I couldn't take it anymore. A few questions, not so much related to your tek, it's pretty much cut and dry, more so just agar related. I took some tissue from a few nice fruits (one from outside that was 136g wet), how long until I start to see noticeable growth? Also, I was having the hardest time getting the tissue sample off of the blade and onto the agar where I wanted it, on a few occasions, I had to touch the blade and drag it to get the tissue off. My blade was flame sterilized, so would this cause any contam issues or is it proper technique to touch and drag like you would with an inoculation loop? Thanks for the tek, it's pretty awesome.
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elasticaltiger
Like Tigers in Coitus




Registered: 06/24/13
Posts: 8,438
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Quote:
MrGiraffe said: Just did my first batch last night. Still waiting on my agar to arrive in the mail, so I bit the bullet and paid $9 for an oz of the food flakes from the only place in town that had it. I had a stack of mini glads staring me down, I couldn't take it anymore. A few questions, not so much related to your tek, it's pretty much cut and dry, more so just agar related. I took some tissue from a few nice fruits (one from outside that was 136g wet), how long until I start to see noticeable growth? Also, I was having the hardest time getting the tissue sample off of the blade and onto the agar where I wanted it, on a few occasions, I had to touch the blade and drag it to get the tissue off. My blade was flame sterilized, so would this cause any contam issues or is it proper technique to touch and drag like you would with an inoculation loop? Thanks for the tek, it's pretty awesome.
I always see tissue start to get fuzzy with growth within 24 - 48 and then start really spreading to the dish after that.
I had the same problem with my samples sticking to my blade when I took them. I think this just takes practice. It doesn't take a lot of tissue to get a clone so maybe try to minimize how deeply you're dragging the blade through the stem? Maybe someone else has a better way though. I haven't seen any increased contamination from dragging the sample onto the agar.
-------------------- First time growing cakes? DON'T make a Shotgun Fruiting Chamber The Shmuvbox. - The Old TC's Like it Afraid to Start Growing From Your Own Prints? Drop it Like a Tiger! No Pouring. No Syringes. No Cutting. No flaming. No Contamination. No Bullshit. "The best thing to do while your waiting is to start more stuff. I usually got so much happening that I have tossed projects simply because I didn't have time for them. -Pastywhite QFT Pastywhite's Easy Agar Tek (PastyPlates) Tiger Drop Video Demos By munchauzen Van Gogh would’ve sold more than one painting if he’d put tigers in them.―Bill Watterson EZEKIEL 23:20
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,887
Loc: Canada
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Dragging the blade is sometimes neccessary, should not cause any issues. I find the tissue sticks more when the blade is old. Try using a fresh blade for cloning.
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MrGiraffe

Registered: 04/04/14
Posts: 3,149
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Quote:
Pastywhyte said: Dragging the blade is sometimes neccessary, should not cause any issues. I find the tissue sticks more when the blade is old. Try using a fresh blade for cloning.
I use an xacto. I have noticed, even when just cutting stems off for printing, that the mushrooms seem to dull the shit out of them pretty quickly. I'll have to keep the blade changed out next time. I've got 8 more miniglads waiting on more agar lol. Can't wait to get the minifridge from my mil to turn into culture storage. Thanks for the info, off to run the pc!!
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,887
Loc: Canada
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Yeah me too. I usually have two on the go, one with a new blade for work that I really want to be precise and an older blade for those in between isolation transfers or spore inoculation where it does not matter much to me whether I gotta drag around or twist the blade to get tissue or wedge off. Though I have a habit of using a blade until its dull as nails anyway. Point is if it gets the job done clean then thats all that matters.
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elasticaltiger
Like Tigers in Coitus




Registered: 06/24/13
Posts: 8,438
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Quote:
Pastywhyte said: Though I have a habit of using a blade until its dull as nails anyway.
I use my scalpel to harvest my tubs.
-------------------- First time growing cakes? DON'T make a Shotgun Fruiting Chamber The Shmuvbox. - The Old TC's Like it Afraid to Start Growing From Your Own Prints? Drop it Like a Tiger! No Pouring. No Syringes. No Cutting. No flaming. No Contamination. No Bullshit. "The best thing to do while your waiting is to start more stuff. I usually got so much happening that I have tossed projects simply because I didn't have time for them. -Pastywhite QFT Pastywhite's Easy Agar Tek (PastyPlates) Tiger Drop Video Demos By munchauzen Van Gogh would’ve sold more than one painting if he’d put tigers in them.―Bill Watterson EZEKIEL 23:20
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blindingleaf
blue collar underworld



Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
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Quote:
elasticaltiger said:
Quote:
Pastywhyte said: Though I have a habit of using a blade until its dull as nails anyway.
I use my scalpel to harvest my tubs. 
i tried that, and 1/4 way thru i gave up and did twst/pull cause it took so long. i use scissors now. fruits def do a number on a blade tho. i mean, we can't even digest chitin, so no wonder it fucks up a blade. wish my skin was made of chitin when i was still shooting. that would have stopped me dead on my "tracks"
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
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elasticaltiger
Like Tigers in Coitus




Registered: 06/24/13
Posts: 8,438
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Quote:
blindingleaf said:wish my skin was made of chitin when i was still shooting. that would have stopped me dead on my "tracks" 
-------------------- First time growing cakes? DON'T make a Shotgun Fruiting Chamber The Shmuvbox. - The Old TC's Like it Afraid to Start Growing From Your Own Prints? Drop it Like a Tiger! No Pouring. No Syringes. No Cutting. No flaming. No Contamination. No Bullshit. "The best thing to do while your waiting is to start more stuff. I usually got so much happening that I have tossed projects simply because I didn't have time for them. -Pastywhite QFT Pastywhite's Easy Agar Tek (PastyPlates) Tiger Drop Video Demos By munchauzen Van Gogh would’ve sold more than one painting if he’d put tigers in them.―Bill Watterson EZEKIEL 23:20
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MrGiraffe

Registered: 04/04/14
Posts: 3,149
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I just used a blade this morning to harvest some...I may go back to the twist and pull, seems to leave the sub a little cleaner. What should i do about the nubbies left over from cutting as opposed to the twist/pull method?
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elasticaltiger
Like Tigers in Coitus




Registered: 06/24/13
Posts: 8,438
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Quote:
MrGiraffe said: I just used a blade this morning to harvest some...I may go back to the twist and pull, seems to leave the sub a little cleaner. What should i do about the nubbies left over from cutting as opposed to the twist/pull method?
Just leave em. After the second flush you can turn the sub over for more room for pins. But the stumps aren't going to affect your next flush.
-------------------- First time growing cakes? DON'T make a Shotgun Fruiting Chamber The Shmuvbox. - The Old TC's Like it Afraid to Start Growing From Your Own Prints? Drop it Like a Tiger! No Pouring. No Syringes. No Cutting. No flaming. No Contamination. No Bullshit. "The best thing to do while your waiting is to start more stuff. I usually got so much happening that I have tossed projects simply because I didn't have time for them. -Pastywhite QFT Pastywhite's Easy Agar Tek (PastyPlates) Tiger Drop Video Demos By munchauzen Van Gogh would’ve sold more than one painting if he’d put tigers in them.―Bill Watterson EZEKIEL 23:20
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XcWABc1
Becoming a Myco Pimp

Registered: 06/06/14
Posts: 34
Loc: HELL
Last seen: 9 years, 8 months
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Re: Pastywhyte's "Easy Agar" Tek [Re: Pastywhyte]
#20113028 - 06/11/14 06:08 AM (10 years, 21 days ago) |
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Pasty do you alway use the micropore tape?
****BOOKMARKED****
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,887
Loc: Canada
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Re: Pastywhyte's "Easy Agar" Tek [Re: XcWABc1]
#20113051 - 06/11/14 06:16 AM (10 years, 21 days ago) |
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I have also used waterproof first aid tape with success (thanks van der grigen) and of course polyfil will work
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blindingleaf
blue collar underworld



Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
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Re: Pastywhyte's "Easy Agar" Tek [Re: Pastywhyte]
#20113235 - 06/11/14 07:35 AM (10 years, 21 days ago) |
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can i store my weed in pasty plates?
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
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MrGiraffe

Registered: 04/04/14
Posts: 3,149
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Re: Pastywhyte's "Easy Agar" Tek [Re: blindingleaf]
#20113254 - 06/11/14 07:45 AM (10 years, 21 days ago) |
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blindingleaf said: can i store my weed in pasty plates?
Only if you put tape over the hole, otherwise the bud will dry out to and when you break it up to toke it will be crumbly mess, to the point that it may break up into powder lol. Once the bud is at proper moisture content, you don't want it exposed to air. If it's some dank, it may permanently make the round smell like bud haha. Edit: by tape, I mean of the nonporous variety.
Edited by MrGiraffe (06/11/14 07:46 AM)
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blindingleaf
blue collar underworld



Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
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Re: Pastywhyte's "Easy Agar" Tek [Re: MrGiraffe]
#20113264 - 06/11/14 07:48 AM (10 years, 21 days ago) |
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i was joking, but ok. i actually do store them in these (i have like 64 of them) but i don't put tape on the hole??? i like my weed super dry, its easier to break up and roll into spliffs. why do u want a certain moisture level in bud? I've never heard that, but then again, i have never grown weed or really got all into cannabis culture like that.
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
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Killaloting
Stranger

Registered: 06/03/14
Posts: 98
Last seen: 4 years, 3 months
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very nice easy to use, working 100% 
do we know what agar does colonize the fastest? Malt or grainwater or potato agar?
If anyone have experienced differences in the time it takes please share
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MrGiraffe

Registered: 04/04/14
Posts: 3,149
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Re: Pastywhyte's "Easy Agar" Tek [Re: blindingleaf]
#20113389 - 06/11/14 08:28 AM (10 years, 21 days ago) |
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blindingleaf said:
i was joking, but ok. i actually do store them in these (i have like 64 of them) but i don't put tape on the hole??? i like my weed super dry, its easier to break up and roll into spliffs. why do u want a certain moisture level in bud? I've never heard that, but then again, i have never grown weed or really got all into cannabis culture like that.
I kind of thought you were, though wouldn't hurt to have a dual purpose for them, I've only got 16...for now lol. In such a small container, it probably doesn't matter, you'll smoke through it in no time, but for longer term storage (like qt jars worth) it'll cure out and develop better taste. Plus if it's bone dry, it'll burn up super fast in a blunt, I like mine to burn long and slow like a real cigar lol.
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