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Invisiblemushmagic
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Re: Pasty Agar Tek [Re: Al Bundy]
    #22176951 - 09/02/15 03:49 AM (8 years, 9 months ago)

Yea it ain't gonna hurt anything it'll just speed things up if anything but yea I feel like it would be better used to expand the mycelium but that's just my opinion:shrug:

Its really up to you tho. A simple "tiger drop" would suffice (and Im assuming that's where you're getting this idea from?)


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OfflineDistrict
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Re: Pasty Agar Tek [Re: mushmagic]
    #22181930 - 09/03/15 07:34 AM (8 years, 9 months ago)

If condensation is the cause of excess water, then why the need to wait for agar to cool before PCing?

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InvisibleInocuole
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Re: Pasty Agar Tek [Re: District]
    #22181965 - 09/03/15 07:55 AM (8 years, 9 months ago)

Because then it stops creating condensation and you can put the cap on?  It's not necessary though, I don't do it.


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                            :rainbowdrink: Tea doesn't work?                            AMU  (Q & A)                  Grain prep for Intergalactic Space Oats :pes:     

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Invisibleinsanemike

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Re: Pasty Agar Tek [Re: Inocuole]
    #22181988 - 09/03/15 08:07 AM (8 years, 9 months ago)

I never wait for the agar to cool before pc'ing.  I always thought that it would cause problems to let the agar cool and solidify before pc'ing.  :shrug:

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OfflineAjahn Don
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Re: Pasty Agar Tek [Re: insanemike]
    #22182012 - 09/03/15 08:15 AM (8 years, 9 months ago)

Quote:

insanemike said:
I never wait for the agar to cool before pc'ing.  I always thought that it would cause problems to let the agar cool and solidify before pc'ing.  :shrug:




I think I'm going this direction next time. I still get condensation, even if I let it cool and harden.

I've saved some grain water to try. One thing I'm wondering: If there is any nutritional benefits to grain water, aren't they likely to be lost when PC'ed?


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Invisibleinsanemike

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Re: Pasty Agar Tek [Re: Ajahn Don]
    #22182022 - 09/03/15 08:20 AM (8 years, 9 months ago)

You don't lose nutrition through a pc cycle, atleast not that I'm aware of. Grain water is probably the single best nutritional source for agar because the mycelium doesn't suffer from a drastic lag phase because it's metabolism doesn't have to change from one media to the another.

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OfflineAjahn Don
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Re: Pasty Agar Tek [Re: insanemike]
    #22182083 - 09/03/15 08:42 AM (8 years, 9 months ago)

Quote:

insanemike said:
You don't lose nutrition through a pc cycle, atleast not that I'm aware of. Grain water is probably the single best nutritional source for agar because the mycelium doesn't suffer from a drastic lag phase because it's metabolism doesn't have to change from one media to the another.




That's good info. I'll keep saving the grain water then. The last absolutely makes sense, to the point of "Duh!" So oat water to oats and WBS water to WBS. Got it.

:smile:


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Invisiblestareatclouds
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Re: Pasty Agar Tek [Re: insanemike]
    #22182087 - 09/03/15 08:45 AM (8 years, 9 months ago)

You just replace the water you'd normally use with the grain water, yeah? Everything else is standard? Karo still?

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Invisiblepablokabute
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Re: Pasty Agar Tek [Re: insanemike]
    #22182095 - 09/03/15 08:47 AM (8 years, 9 months ago)

Quote:

insanemike said:
You don't lose nutrition through a pc cycle, atleast not that I'm aware of. Grain water is probably the single best nutritional source for agar because the mycelium doesn't suffer from a drastic lag phase because it's metabolism doesn't have to change from one media to the another.





then how come I hear something like... to keep the vigor of your culture and avoid senescence, you shift your media from one to the other especially when doing long term storage?


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Invisiblepablokabute
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Re: Pasty Agar Tek [Re: stareatclouds]
    #22182110 - 09/03/15 08:51 AM (8 years, 9 months ago)

Quote:

stareatclouds said:
You just replace the water you'd normally use with the grain water, yeah? Everything else is standard? Karo still?





to your desired dilution... nothing else. you may try dropping some food coloring in there too for some agar work..


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Invisibleinsanemike

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Re: Pasty Agar Tek [Re: pablokabute]
    #22182159 - 09/03/15 09:15 AM (8 years, 9 months ago)

Quote:

pablokabute said:
Quote:

insanemike said:
You don't lose nutrition through a pc cycle, atleast not that I'm aware of. Grain water is probably the single best nutritional source for agar because the mycelium doesn't suffer from a drastic lag phase because it's metabolism doesn't have to change from one media to the another.





then how come I hear something like... to keep the vigor of your culture and avoid senescence, you shift your media from one to the other especially when doing long term storage?




Of course over time you want to change nutritional sources. It's just the same as if you only ate foods that didnt contain iron. It wouldn't effect your health (vigor) right away but over time you would start to feel the effects of iron deficiency.

Edited by insanemike (09/03/15 09:16 AM)

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Invisiblestareatclouds
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Re: Pasty Agar Tek [Re: pablokabute]
    #22182163 - 09/03/15 09:18 AM (8 years, 9 months ago)

Quote:

pablokabute said:
Quote:

stareatclouds said:
You just replace the water you'd normally use with the grain water, yeah? Everything else is standard? Karo still?





to your desired dilution... nothing else. you may try dropping some food coloring in there too for some agar work..




My agar is red so I don't need food coloring. I'm saying in Pasty's recipe I just replace the water with my grain water.

Quote:

then how come I hear something like... to keep the vigor of your culture and avoid senescence, you shift your media from one to the other especially when doing long term storage?




I think that's referring to multiple transfers of the same culture. So like switching it up to PDE, LMEA, etc.

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InvisibleGrey
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Re: Pasty Agar Tek [Re: stareatclouds]
    #22182203 - 09/03/15 09:35 AM (8 years, 9 months ago)

Stare, you don't have to add anything else to grain water agar. Just the grain water and agar.


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Invisiblestareatclouds
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Re: Pasty Agar Tek [Re: Grey]
    #22182421 - 09/03/15 10:32 AM (8 years, 9 months ago)

Okay, sweet. I figured Karo might make it too nutritious or whatever. I'm gonna make some soon with the last of my agar bar.

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OfflinePinPornProducer
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Re: Pasty Agar Tek [Re: stareatclouds]
    #22182431 - 09/03/15 10:34 AM (8 years, 9 months ago)

Yea too much karo will make the myc way too comfortable and cause it to grow soo fkn slow. My last batch of plates were too sweet and they took forever

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InvisibleMunchauzen
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Re: Pasty Agar Tek [Re: insanemike]
    #22182951 - 09/03/15 12:52 PM (8 years, 9 months ago)

Quote:

insanemike said:
I never wait for the agar to cool before pc'ing.  I always thought that it would cause problems to let the agar cool and solidify before pc'ing.  :shrug:




I saw somebody claim that letting your plates cool before PC will get rid of condensation after the PC.

I did a bunch of plates and let them cool, but they still got condensation. So I think there are still some factors to be worked into this equation.

Edited by Munchauzen (09/03/15 01:10 PM)

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OfflinePinPornProducer
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Re: Pasty Agar Tek [Re: Munchauzen]
    #22183003 - 09/03/15 01:02 PM (8 years, 9 months ago)

It's how I do mine, but you also have to let them cool for about 30 min in the pc after the pressure has dropped. Then detatch the lid and slide it off the pc just slightly so you have about a 1/2" gap and let them cool to room temp that way. The condensation is very minimal

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OfflinePinPornProducer
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Re: Pasty Agar Tek [Re: PinPornProducer]
    #22183120 - 09/03/15 01:28 PM (8 years, 9 months ago)

Have you tried glass yet munch?

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InvisibleMunchauzen
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Re: Pasty Agar Tek [Re: PinPornProducer]
    #22183129 - 09/03/15 01:30 PM (8 years, 9 months ago)

no I just have rubbermaid containers

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OfflinePinPornProducer
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Re: Pasty Agar Tek [Re: Munchauzen]
    #22183141 - 09/03/15 01:33 PM (8 years, 9 months ago)

You should give glass a shot once, I think the thickness of them help with condensation also

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