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mushmagic
supporting radical habits



Registered: 03/21/12
Posts: 5,372
Loc: Candyland
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Re: Pasty Agar Tek [Re: Al Bundy]
#22176951 - 09/02/15 03:49 AM (8 years, 9 months ago) |
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Yea it ain't gonna hurt anything it'll just speed things up if anything but yea I feel like it would be better used to expand the mycelium but that's just my opinion
Its really up to you tho. A simple "tiger drop" would suffice (and Im assuming that's where you're getting this idea from?)
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Trade list in journal (partially under construction; more to be added) Don't judge a man by what kinda shoes he in, judge a man on where that man's shoes been.
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District
Strange


Registered: 07/12/15
Posts: 396
Loc: Within Sector
Last seen: 1 month, 15 days
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If condensation is the cause of excess water, then why the need to wait for agar to cool before PCing?
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,867
Loc: ★
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Re: Pasty Agar Tek [Re: District]
#22181965 - 09/03/15 07:55 AM (8 years, 9 months ago) |
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Because then it stops creating condensation and you can put the cap on? It's not necessary though, I don't do it.
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insanemike

Registered: 02/23/14
Posts: 4,272
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Re: Pasty Agar Tek [Re: Inocuole]
#22181988 - 09/03/15 08:07 AM (8 years, 9 months ago) |
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I never wait for the agar to cool before pc'ing. I always thought that it would cause problems to let the agar cool and solidify before pc'ing.
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Ajahn Don
Stranger


Registered: 07/05/15
Posts: 482
Loc: The buckle of the Bible belt
Last seen: 1 year, 2 months
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Quote:
insanemike said: I never wait for the agar to cool before pc'ing. I always thought that it would cause problems to let the agar cool and solidify before pc'ing. 
I think I'm going this direction next time. I still get condensation, even if I let it cool and harden.
I've saved some grain water to try. One thing I'm wondering: If there is any nutritional benefits to grain water, aren't they likely to be lost when PC'ed?
-------------------- "He's not altogether dense, but he's not altogether there."
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insanemike

Registered: 02/23/14
Posts: 4,272
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You don't lose nutrition through a pc cycle, atleast not that I'm aware of. Grain water is probably the single best nutritional source for agar because the mycelium doesn't suffer from a drastic lag phase because it's metabolism doesn't have to change from one media to the another.
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Ajahn Don
Stranger


Registered: 07/05/15
Posts: 482
Loc: The buckle of the Bible belt
Last seen: 1 year, 2 months
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Quote:
insanemike said: You don't lose nutrition through a pc cycle, atleast not that I'm aware of. Grain water is probably the single best nutritional source for agar because the mycelium doesn't suffer from a drastic lag phase because it's metabolism doesn't have to change from one media to the another.
That's good info. I'll keep saving the grain water then. The last absolutely makes sense, to the point of "Duh!" So oat water to oats and WBS water to WBS. Got it.
-------------------- "He's not altogether dense, but he's not altogether there."
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stareatclouds
star eat clouds?



Registered: 09/29/14
Posts: 10,329
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You just replace the water you'd normally use with the grain water, yeah? Everything else is standard? Karo still?
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pablokabute
Hari ng Amag



Registered: 11/22/11
Posts: 5,191
Loc: rural ghetto
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Quote:
insanemike said: You don't lose nutrition through a pc cycle, atleast not that I'm aware of. Grain water is probably the single best nutritional source for agar because the mycelium doesn't suffer from a drastic lag phase because it's metabolism doesn't have to change from one media to the another.
then how come I hear something like... to keep the vigor of your culture and avoid senescence, you shift your media from one to the other especially when doing long term storage?
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Fermented Mushrooms!! --- https://www.shroomery.org/forums/showflat.php/Number/23378638/fpart/1/vc/1 'The second seal: “All CONTAMINATED things and events are unsatisfactory.”' "I envy you. You North Americans are very lucky. You are fighting the most important fight of all - you live in THE HEART OF THE BEAST." --Anonymous Guerilla, or is he..
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pablokabute
Hari ng Amag



Registered: 11/22/11
Posts: 5,191
Loc: rural ghetto
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Quote:
stareatclouds said: You just replace the water you'd normally use with the grain water, yeah? Everything else is standard? Karo still?
to your desired dilution... nothing else. you may try dropping some food coloring in there too for some agar work..
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Fermented Mushrooms!! --- https://www.shroomery.org/forums/showflat.php/Number/23378638/fpart/1/vc/1 'The second seal: “All CONTAMINATED things and events are unsatisfactory.”' "I envy you. You North Americans are very lucky. You are fighting the most important fight of all - you live in THE HEART OF THE BEAST." --Anonymous Guerilla, or is he..
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insanemike

Registered: 02/23/14
Posts: 4,272
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Quote:
pablokabute said:
Quote:
insanemike said: You don't lose nutrition through a pc cycle, atleast not that I'm aware of. Grain water is probably the single best nutritional source for agar because the mycelium doesn't suffer from a drastic lag phase because it's metabolism doesn't have to change from one media to the another.
then how come I hear something like... to keep the vigor of your culture and avoid senescence, you shift your media from one to the other especially when doing long term storage?
Of course over time you want to change nutritional sources. It's just the same as if you only ate foods that didnt contain iron. It wouldn't effect your health (vigor) right away but over time you would start to feel the effects of iron deficiency.
Edited by insanemike (09/03/15 09:16 AM)
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stareatclouds
star eat clouds?



Registered: 09/29/14
Posts: 10,329
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Quote:
pablokabute said:
Quote:
stareatclouds said: You just replace the water you'd normally use with the grain water, yeah? Everything else is standard? Karo still?
to your desired dilution... nothing else. you may try dropping some food coloring in there too for some agar work..
My agar is red so I don't need food coloring. I'm saying in Pasty's recipe I just replace the water with my grain water.
Quote:
then how come I hear something like... to keep the vigor of your culture and avoid senescence, you shift your media from one to the other especially when doing long term storage?
I think that's referring to multiple transfers of the same culture. So like switching it up to PDE, LMEA, etc.
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Grey
⇜ ✯ ⇝



Registered: 11/06/14
Posts: 6,223
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Stare, you don't have to add anything else to grain water agar. Just the grain water and agar.
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AMU Q&A If you don't have a plan of your own, you'll become a part of somebody else's.
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stareatclouds
star eat clouds?



Registered: 09/29/14
Posts: 10,329
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Re: Pasty Agar Tek [Re: Grey]
#22182421 - 09/03/15 10:32 AM (8 years, 9 months ago) |
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Okay, sweet. I figured Karo might make it too nutritious or whatever. I'm gonna make some soon with the last of my agar bar.
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PinPornProducer
Buy the ticket, take the ride



Registered: 08/23/14
Posts: 9,981
Loc: Rocky Point R.I
Last seen: 6 years, 5 months
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Yea too much karo will make the myc way too comfortable and cause it to grow soo fkn slow. My last batch of plates were too sweet and they took forever
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Munchauzen


Registered: 06/22/11
Posts: 14,346
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Quote:
insanemike said: I never wait for the agar to cool before pc'ing. I always thought that it would cause problems to let the agar cool and solidify before pc'ing. 
I saw somebody claim that letting your plates cool before PC will get rid of condensation after the PC.
I did a bunch of plates and let them cool, but they still got condensation. So I think there are still some factors to be worked into this equation.
Edited by Munchauzen (09/03/15 01:10 PM)
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PinPornProducer
Buy the ticket, take the ride



Registered: 08/23/14
Posts: 9,981
Loc: Rocky Point R.I
Last seen: 6 years, 5 months
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It's how I do mine, but you also have to let them cool for about 30 min in the pc after the pressure has dropped. Then detatch the lid and slide it off the pc just slightly so you have about a 1/2" gap and let them cool to room temp that way. The condensation is very minimal
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PinPornProducer
Buy the ticket, take the ride



Registered: 08/23/14
Posts: 9,981
Loc: Rocky Point R.I
Last seen: 6 years, 5 months
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Have you tried glass yet munch?
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Munchauzen


Registered: 06/22/11
Posts: 14,346
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no I just have rubbermaid containers
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PinPornProducer
Buy the ticket, take the ride



Registered: 08/23/14
Posts: 9,981
Loc: Rocky Point R.I
Last seen: 6 years, 5 months
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You should give glass a shot once, I think the thickness of them help with condensation also
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