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Invisiblepablokabute
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Registered: 11/22/11
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Re: Pasty Agar Tek [Re: blackdust]
    #22135525 - 08/24/15 01:43 PM (8 years, 10 months ago)

Quote:

blackdust said:
Quote:

PinPornProducer said:
Quote:

newera said:
i can't find these  things around town anymore(the pasty plates) so i'm going with glass jars i guess



Glass 1/2 pints work great






YEAH! I love them.






Neat!


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--- https://www.shroomery.org/forums/showflat.php/Number/23378638/fpart/1/vc/1

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Offlineaerow.thefox
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Re: Pasty Agar Tek [Re: pablokabute]
    #22138145 - 08/24/15 11:15 PM (8 years, 10 months ago)

how feasable is it to use syriniges to TAKE the cuttings from the plates? i read a tek about taking clones with thick guage needles and doing a 'biopsy'. could you scrape and suck into sterile water?
i like using tyvek and was wondering if i could make some agar dishes in the squat 1/2 pints or 1/4 pints with tyvek lids. i also put injection ports on my tyvek. soooo...  totally sterile; never open air conditions from right out the pc to inject to taking a cut. always filtered air
what do you guys think? possible? or would you still be better opening it and making the cut with a sterile scalpel/equivalent? getting ideas.. thx guys!


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Edited by aerow.thefox (08/24/15 11:18 PM)

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InvisibleInocuole
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Re: Pasty Agar Tek [Re: aerow.thefox]
    #22138169 - 08/24/15 11:23 PM (8 years, 10 months ago)

I would only use tyvek if that's all I had.  I don't quite understand how you can "like" using tyvek.  Either you haven't tried SFDs or you've got stockholm syndrome. :shrug:  That said for this tek I'm sure tyvek is fine if micropore tape is fine, but I would never recommend tyvek for grain jars long-term.  One or two uses is about all I'd wager anything on.

If you're asking if you can use the syringe needle to suck up a slice of scraped off mycelium, sure, and there's some people who've done variations of that like make liquid inoculants by pressure-spraying the mycelium off of the agar and sucking it back up, but I would still recommend getting in some practice with a blade.  You can't be very accurate with a liquid when making transfers, and using liquids where taking clones is concerned is also dangerous.


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Invisiblepablokabute
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Re: Pasty Agar Tek [Re: Inocuole]
    #22138328 - 08/25/15 12:23 AM (8 years, 10 months ago)

Quote:

Inocuole said:
I would only use tyvek if that's all I had.  I don't quite understand how you can "like" using tyvek.  Either you haven't tried SFDs or you've got stockholm syndrome. :shrug:  That said for this tek I'm sure tyvek is fine if micropore tape is fine, but I would never recommend tyvek for grain jars long-term.  One or two uses is about all I'd wager anything on.

If you're asking if you can use the syringe needle to suck up a slice of scraped off mycelium, sure, and there's some people who've done variations of that like make liquid inoculants by pressure-spraying the mycelium off of the agar and sucking it back up, but I would still recommend getting in some practice with a blade.  You can't be very accurate with a liquid when making transfers, and using liquids where taking clones is concerned is also dangerous.



:takingnotes:


--------------------

Fermented Mushrooms!!
--- https://www.shroomery.org/forums/showflat.php/Number/23378638/fpart/1/vc/1

'The second seal: “All CONTAMINATED things and events are unsatisfactory.”'



"I envy you. You North Americans are very lucky. You are fighting the most important fight of all - you live in THE HEART OF THE BEAST."

--Anonymous Guerilla, or is he..

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OfflineMykoMyers
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Re: Pasty Agar Tek [Re: Inocuole]
    #22138585 - 08/25/15 02:56 AM (8 years, 10 months ago)

Know a good sfd tek?


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Invisibleinsanemike

Registered: 02/23/14
Posts: 4,272
Re: Pasty Agar Tek [Re: MykoMyers]
    #22138663 - 08/25/15 03:28 AM (8 years, 10 months ago)

I've been using tyvek almost exclusively for the past 2 years and have never seen a problem that I could trace back to using it. I have been testing both sfd's and dupont tyvek suits side by side for the past month because I heard this same thing about sfd's being superior to tyvek. I just had to see what all of the fuss was about. I thought maybe sfd's gave better performance, as far as colonization times or something. Honestly, I have seen no difference in performance or any increase in contam rates with either one.

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Offlineaerow.thefox
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Re: Pasty Agar Tek [Re: insanemike]
    #22139850 - 08/25/15 09:05 AM (8 years, 10 months ago)

yea any problems that arise from mine never have to do with the tyvek. iv never had a problem. i re-use mine as well. rtv silicone sticks to the tops of it if you work it around a lil. my tyvek grain lids have never steered me wrong. and i can use them 4 or 5 times before i feel there is starting to be wear. of course anything that DOES go bad is thrown out with the lid. but any uncontaminated tyvek spawn lids go right back to use in the next grow. :p


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OfflineDistrict
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Re: Pasty Agar Tek [Re: aerow.thefox]
    #22158978 - 08/29/15 12:25 AM (8 years, 9 months ago)

Condensation is killing my pastyplates (I live in a tropical country), turning into excess water.

I'm trying to control temperature fluctuation by chucking the next batch into a portable igloo cooler. Will this work?

Also, can I reuse my agar-filled pasty plates with contamination,by chucking them back into the PC?

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Invisiblestareatclouds
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Re: Pasty Agar Tek [Re: District]
    #22159028 - 08/29/15 12:42 AM (8 years, 9 months ago)

The condensation shouldn't be too much of a problem unless it's flooding them. And even then, I'm not convinced it's awful for them.

Also taking hot plates and tossing them in something cold is going to make them sweat WAY more. Condensation is a temperature difference between 2 things. I'm pretty sure slowly rising and lowering the temperatures is all that's going to help. What I used to do was take the plates out of the cooled PC (talking after 10 hours or something overnight) straight into the SAB. Using clean technique (iso, gloves, mask, all the shit), I'd take off the foil and paper towel, open the lid, and wipe out the condensation. Then I'd put the paper towel back and move onto the next one. Didn't see my contams increase.

And nah man, just toss them out and make new agar.

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OfflineDistrict
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Re: Pasty Agar Tek [Re: District]
    #22159091 - 08/29/15 01:11 AM (8 years, 9 months ago)

It floods them. And the condensation forms at incubation, not during cooling. Not sure if it is a factor, but we have very high levels of humidity right now.

So far, I would wipe the excess condensation at room temperature, and 24 hours later, more condensation would form and cover the surface.

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InvisibleInocuole
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Re: Pasty Agar Tek [Re: District]
    #22159116 - 08/29/15 01:26 AM (8 years, 9 months ago)

It'll all dry up within 2 weeks or so.  That happens with no pour sometimes.  Use less water is all I can recommend.  Or, more agar.


--------------------
                            :rainbowdrink: Tea doesn't work?                            AMU  (Q & A)                  Grain prep for Intergalactic Space Oats :pes:     

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Invisiblestareatclouds
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Re: Pasty Agar Tek [Re: Inocuole]
    #22159157 - 08/29/15 01:58 AM (8 years, 9 months ago)

Please specify what you mean by incubation.

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OfflineDistrict
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Re: Pasty Agar Tek [Re: stareatclouds]
    #22159168 - 08/29/15 02:07 AM (8 years, 9 months ago)

colonization of agar media.

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InvisibleInocuole
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Re: Pasty Agar Tek [Re: District]
    #22159173 - 08/29/15 02:10 AM (8 years, 9 months ago)

But there's no actual.. incubating taking place right?


--------------------
                            :rainbowdrink: Tea doesn't work?                            AMU  (Q & A)                  Grain prep for Intergalactic Space Oats :pes:     

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OfflineDistrict
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Re: Pasty Agar Tek [Re: Inocuole]
    #22159174 - 08/29/15 02:12 AM (8 years, 9 months ago)

I've played with the mixture. Condensation occurs after it is dry (from me wiping it when it cools), which was why I thought about temperature fluctuation of the room as cause. A storm forms on a sunny day,and temperature will plummet,then later on, storm dissipates and sun comes out. Temp fluctuates quickly in  a tropical country.

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OfflineDistrict
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Re: Pasty Agar Tek [Re: Inocuole]
    #22159179 - 08/29/15 02:14 AM (8 years, 9 months ago)

There is. I meant after I wedge a mushroom in (im using wild btw). Ill try to play around without mushrooms, just to see. Bacteria likes water, I read, so theres that, but thats from another issue.

Edited by District (08/29/15 02:15 AM)

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InvisibleThoky.ykoht

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Re: Pasty Agar Tek [Re: District]
    #22176543 - 09/01/15 11:45 PM (8 years, 9 months ago)

I've been using these for a while and always end up with extra that goes to waste. I was curious if you can use more than one plate per jar?

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Invisiblestareatclouds
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Re: Pasty Agar Tek [Re: Thoky.ykoht]
    #22176781 - 09/02/15 01:50 AM (8 years, 9 months ago)

District,

Incubating is you controlling temperature, usually warmer than normal, to grow stuff. You're referring to colonization, which is your mycelium colonizing the agar plate. It's confusing when you mix them up.

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Offlineaykaye47
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Re: Pasty Agar Tek [Re: stareatclouds]
    #22176905 - 09/02/15 03:16 AM (8 years, 9 months ago)

I got some of these containers from work. Its a pp5 container that electrical tape comes in I got about 6 of them I wonder if they would work good or if they would spill out in the pc they have a snap lid but its not air tight


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OfflineAl Bundy
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Re: Pasty Agar Tek [Re: Thoky.ykoht]
    #22176922 - 09/02/15 03:25 AM (8 years, 9 months ago)

Quote:

Thoky.ykoht said:
I've been using these for a while and always end up with extra that goes to waste. I was curious if you can use more than one plate per jar?



More than one agar plate per jar? I mean you could.
Why not just stick plates that haven't been nocd up in the fridge? Or just don't make so many plates in the first place...

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