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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,867
Loc: ★
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Re: Pasty Agar Tek [Re: MudaFuka]
#21464462 - 03/27/15 07:54 AM (9 years, 3 months ago) |
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Quote:
MudaFuka said: Wiping down a sterilised plate is a bad idea.
Quote:
MudaFuka said: Wiping down a sterilised plate is a bad idea.
SAID IT ONCE BEFORE BUT IT BEARS REPEATING NOW
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,887
Loc: Canada
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Re: Pasty Agar Tek [Re: Inocuole]
#21464508 - 03/27/15 08:12 AM (9 years, 3 months ago) |
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I have yet to get a plate where any condesation does not clear in a few days. Regardless condesation is not a contam vector so its not much to worry about. I get more condensation with pour agar than I do with these. I should put that in the OP.
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blindingleaf
blue collar underworld


Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
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in the first 10-15 pages on this thread, i mentioned i was using that paper towel on top of lid as a way to wipe condensation 
its sterilized with the whole lot. i never had a problem.
-------------------- A few thoughts on cultivation MICROBIAL HUSBANDRY!!!! The whole is greater than the sum of its parts
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hamloaf
Pork Block ®©™√


Registered: 12/23/09
Posts: 22,071
Loc: ation undisclosed.
Last seen: 41 minutes, 54 seconds
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Condensation along where the agar vessel closes CAN be a vector for contamination, so be advised. Glad you guys don't have any issues with condensation when it arises. Looks like you all know what to do about condensation.
I keep condensation under control by keeping the spawning area at a constant 75F. Would be best if the RH of the lab would stay a constant RH of 50%, but when the flowhood runs there's a 30-35% RH swing in favor of when the flowhood runs as opposed to when the flowhood is not running. Keeping the temperature constant is no problem with a digital thermostat and small space heater.
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,867
Loc: ★
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Re: Pasty Agar Tek [Re: hamloaf]
#21464563 - 03/27/15 08:33 AM (9 years, 3 months ago) |
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I shake up the dish before I open it so that the condensation is NOT up along the upper edge. It'll all pool in one spot and I can dump it away.
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,893
Loc: Milky way
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Re: Pasty Agar Tek [Re: hamloaf]
#21465897 - 03/27/15 02:57 PM (9 years, 3 months ago) |
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Quote:
hamloaf said: Condensation along where the agar vessel closes CAN be a vector for contamination, so be advised. Glad you guys don't have any issues with condensation when it arises. Looks like you all know what to do about condensation.
I keep condensation under control by keeping the spawning area at a constant 75F. Would be best if the RH of the lab would stay a constant RH of 50%, but when the flowhood runs there's a 30-35% RH swing in favor of when the flowhood runs as opposed to when the flowhood is not running. Keeping the temperature constant is no problem with a digital thermostat and small space heater.
be advised of what exactly ham? condensation inside of a dish that hasn't been exposed to any vector for contamination?
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Pyrrho
Smiling Misanthrope



Registered: 01/30/14
Posts: 78
Loc: Cloud City
Last seen: 8 years, 11 months
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This tek has inspired me to take the dive into liquid cultures. Thanks Pastywhyte. Finishing off the last of my PF jars and never looking back!
-------------------- "Change alone is eternal, perpetual, immortal." ~Arthur Schopenhauer
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blackdust


Registered: 02/28/09
Posts: 8,327
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Quote:
bodhisatta said:
Quote:
hamloaf said: Condensation along where the agar vessel closes CAN be a vector for contamination, so be advised. Glad you guys don't have any issues with condensation when it arises. Looks like you all know what to do about condensation.
I keep condensation under control by keeping the spawning area at a constant 75F. Would be best if the RH of the lab would stay a constant RH of 50%, but when the flowhood runs there's a 30-35% RH swing in favor of when the flowhood runs as opposed to when the flowhood is not running. Keeping the temperature constant is no problem with a digital thermostat and small space heater.
be advised of what exactly ham? condensation inside of a dish that hasn't been exposed to any vector for contamination?
dishes are not vacuum sealed and water can provide a path from outside the dish to inside the dish as water is an efficient way for microorganisms to move.
Quote:
2.5 Contamination from Water Water is a very serious source of microbiology contamination. Microorganisms, particularly Gram-negative bacteria, can grow and multiply in water, even when nutrients are only present in very low concentrations. Some such microorganisms evolve to form films or slime's, which absorb nutrients from flowing water. Periodically these films are naturally sloughed off into the water system.
Most microorganisms are unable to move or expand more than a few millimeters on dry, solid surfaces. Conversely, waterborne types are guaranteed to be found in practically every wet location, and in locations that have recently been wetted. Water is therefore a vector, as well as a source, of microbiological contamination.
"Microbiological Contamination Control in Pharmaceutical Clean Rooms - Nigel Halls"
To bad you cant see this to reply to my statement you may have actually made a decent counter point with supported documentation. But that would require you to read comments that challenge your view on cultivation and may show you could be wrong on a point or two. Ego's really suck sometimes.
Edited by blackdust (03/27/15 03:49 PM)
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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,867
Loc: ★
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Re: Pasty Agar Tek [Re: Pyrrho]
#21466257 - 03/27/15 04:21 PM (9 years, 3 months ago) |
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Quote:
Pyrrho said: This tek has inspired me to take the dive into liquid cultures. Thanks Pastywhyte. Finishing off the last of my PF jars and never looking back!
Not picking apart your post, or maybe I am, but uh.... What does this thread have to do with making LC?
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eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
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Re: Pastywhyte's Easy Agar Tek [Re: Inocuole] 2
#21484457 - 03/31/15 05:54 PM (9 years, 2 months ago) |
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so check it out.
these are two plates, one blue oyster, one pearl oyster from 2007 syringes. they were stored in the fridge in a liquid culture syringe since then. and they colonized in a few days. i used about a drop of inoculant. i haven't transferred them yet as ive been busy. i DID NOT flame sterilize the syringe. i didn't touch it on anything either. just squirted one squirt then put the tip back on. ive also used both of these sryinges for a few grows over the years. i just used a few cc's to some grain the last few times i grew out oysters.
in addition. i put some spores from 2003 on a few plates and they germinated! and no visible contams.
going to try and do transfers soon. i have some old things im trying to clean up but so far everything germinated over 5 years old!
bringing out the old spore book. first time i used my sab in over 8 years lmao. im real excited about my old stropharia and treasure coast clones. the stropharia was from around 2003 when i was doing mostly dub tubs.
Edited by eatyualive (03/31/15 05:55 PM)
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hamloaf
Pork Block ®©™√


Registered: 12/23/09
Posts: 22,071
Loc: ation undisclosed.
Last seen: 41 minutes, 54 seconds
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Re: Pastywhyte's Easy Agar Tek [Re: eatyualive]
#21484493 - 03/31/15 06:02 PM (9 years, 2 months ago) |
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cArcace-x
YeAh!!



Registered: 10/17/13
Posts: 2,412
Loc: The Simulator
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Re: Pastywhyte's Easy Agar Tek [Re: hamloaf]
#21484700 - 03/31/15 06:46 PM (9 years, 2 months ago) |
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an 8 years old LC!!  BAM!!
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,887
Loc: Canada
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Re: Pastywhyte's Easy Agar Tek [Re: cArcace-x]
#21484718 - 03/31/15 06:48 PM (9 years, 2 months ago) |
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That is fucking incredible 
Also I usually don't bother to flame the needle when inoculating these. Hell sometimes I don't even screw one on to begin with if its a fresh syringe from a sponsor
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eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
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Re: Pastywhyte's Easy Agar Tek [Re: Pastywhyte]
#21484867 - 03/31/15 07:27 PM (9 years, 2 months ago) |
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nice pasty. the liquid cultures were from a trusted vendor. the stropharia spores were taken by me. but hell ive had 5 year old prints do absolutely nothing. i had a last ditch effort with that stropharia. its a real old one check it out. pictures from 2003 of stropharia. at least that is what they were called.

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DirdyD



Registered: 12/01/14
Posts: 1,650
Last seen: 3 years, 5 months
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Re: Pastywhyte's Easy Agar Tek [Re: eatyualive]
#21496698 - 04/03/15 02:35 PM (9 years, 2 months ago) |
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Hay Pasty.
Starting your tek today. Thanks!
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TheEaglesGift
The Nagual


Registered: 04/10/11
Posts: 10,554
Loc: Ixtlan, Mexico
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Re: Pastywhyte's Easy Agar Tek [Re: DirdyD]
#21496951 - 04/03/15 03:44 PM (9 years, 2 months ago) |
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Quote:
DirdyD said: Hay Pasty.
Starting your tek today. Thanks!
Make sure you measure your amounts very well, or you could be stuck with very rock hard or very goopy agar, which you wouldn't want.
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Edmunter
Mr



Registered: 05/01/13
Posts: 5,699
Last seen: 1 month, 13 days
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So every now and again using my grain water 1% agar mix I get a few plates that have excess growth. Almost like a wave of myc growth. Im thinking its too many nutes from the grainwater or worst case scenario a contamination. Here are some bad pictures to try to explain what I mean.

Has anyone had this before and what does it mean. The mycelium looks very agressive it those areas
Edited by Edmunter (04/04/15 05:17 AM)
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DirdyD


Registered: 12/01/14
Posts: 1,650
Last seen: 3 years, 5 months
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Hey Eagle. Any tips other than what is covered in the OP?
Pasty?
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TheEaglesGift
The Nagual


Registered: 04/10/11
Posts: 10,554
Loc: Ixtlan, Mexico
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Re: Pastywhyte's Easy Agar Tek [Re: DirdyD] 1
#21504928 - 04/05/15 03:24 AM (9 years, 2 months ago) |
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Don't make it too thick, don't make it too thin.
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Edmunter
Mr



Registered: 05/01/13
Posts: 5,699
Last seen: 1 month, 13 days
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Re: Pastywhyte's Easy Agar Tek [Re: Edmunter]
#21504945 - 04/05/15 03:38 AM (9 years, 2 months ago) |
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Quote:
Edmunter said: So every now and again using my grain water 1% agar mix I get a few plates that have excess growth. Almost like a wave of myc growth. Im thinking its too many nutes from the grainwater or worst case scenario a contamination. Here are some bad pictures to try to explain what I mean.

Has anyone had this before and what does it mean. The mycelium looks very agressive it those areas
I ment to ask would you use this for a project?
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