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InvisiblePastywhyteMDiscord
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Pastywhyte's Easy Agar Tek * 197
    #19208976 - 11/30/13 03:10 PM (10 years, 2 months ago)

Easy Agar Tek.

I'm starting to see an abundance of people out there who want to get into agar but are intimidated by the thought of pouring petris in a Still Air Box (SAB). There are others who are confident in their technique but don't like the idea of ordering lab equipment off the net to their house. Others still, dislike the idea of waste and having suspicious shit in their trash.

To get around this people are turning to no pour agar teks, and there are many good ones out there, they served the basis of how I myself got started with agar. But most of them have drawbacks. Jars are costly, half pints are too deep to work in with ease, fancy jelly jars are unwieldy to handle and quite expensive. The screwtop ziplocks would be a good alternative except they are large and take up a lot of space in the Pressure Cooker (PC) and fridge.

Enter "Easy Agar". I am here to say that none of the above mentioned drawbacks should dissuade anyone from getting into agar. In my opinion, agar does not need to be difficult, costly, or create a lot of suspicious waste. Many of the pro's and TC's will most likely flame this tek but, its not being written for them. This is for the new cultivator who wants to experience the benefits of agar without the cost and having to order stuff off the net. Let me say that I do use pour agar and proper petris, but for the lions share of my culture work this tek has served me well for 100's of plates.

My PP5 glad mini rounds are much smaller than 1/2 pints and screw top PP5, easier to manipulate in the SAB, and can even be made with a ghetto ass PC that you might have got as a wedding present and never needed or used (like the one I am using for this tek :lol:)

You will need:
-1 pack of Glad mini rounds
-micro pore tape
-a drill with a 1/4" bit or very hot nail
-paper towels
-Foil
-PC
-Bar of agar agar
-Potato flakes
-Karo or Honey
-Coffee grinder
-And of course a PC, even a shitty ass one will work





First we will prep the lids. They need a hole in them, to provide a bit of Gas Exchance (GE) as well as to prevent a vacuum when its PCing from crushing in the mini round container. You will need to drill a small hole in each lid. You can also just heat a paper clip or nail and use it to melt a hole in the lid, this is pretty quick. The hole does not need to be large, a 1/4" is best. Next cover the hole with a small square or two of micro pore tape. If I am planning to keep the plate around for a long time (more than a month) I would suggest two layers. Ok we're done, these are ready to fill :thumbup:





Now we need to prep our agar. Take the bar of agar agar and cut it into small chunks, then place them into the coffee grinder. Grind them until they are pretty fine. Don't worry about making them into powder, just get them pretty uniform. I have even used scissors to get er done, though a coffee grinder is easier. I prefer to grind up my bars and then just store the flakes for when I need them. 2 bars will go a long way. Feel free to use any other agars if you want, powdered agar packets works excellent, just make sure there is no added sugar.





I then spoon 2 heaping tablespoons into the pot. I rarely weigh it out, if I want stiffer agar I use a little more, if I want softer I use a little less. If you want to weigh it, for this amount of agar you will want about 2-2.5 grams depending on how soft or firm you want it to be. If your using powdered agar and don't have a scale, a level tsp (5ml) is roughly 2.5 grams. Remember that no matter how much you want to mix up, you want a ratio of 20-25 grams of agar to 1 liter of water. If you have issues with the mix being too stiff while mixing then adjust the ratios for less agar. Some food grade agars will work out to be a little stiffer than others, some experimentation may be required.

If all that is a bit confusing the just remember 2% is roughly what you are aiming for.

Add 2 teaspoons (10 ml) of potato flakes, and 1/2 cup (120 ml) of water (tap is fine). Then I turn the heat on low on the stove a stir the mixture until its mostly dissolved. At this point I will add a drop of Karo or Honey. Stir it all up well. It does not matter if there are still a few pieces of agar still not dissolved, just get most of it. I like to use either colored agar (some places have this) or else just add a few drops of food coloring to the mix. This will help you see growth real quick, and the contams are easily spotted as well. There will be nowhere for them to hide :lol: If you can only get regular agar, I like to use green food coloring to make the contams stand out best, but any dark color (red, blue, purple) will work as well :thumbup:





Finally I spoon in the agar mix into my glad mini rounds. I like to just give them a wipe with a damp paper towel first to clear out any dust or debris. No need to be spraying them with alcohol or nothing. Place the lids on the top and close. Finally I like to fold up a square of paper towel and place on top. Condensation can be a bit of an issue and you don't want to get the micro pore tape too wet. The paper towel helps with this. Here is the agar in the PP5's and how I do the paper towel.





Now we wrap them in foil before we place in the PC. I like to tear off a piece that is rather square, this will be a good size. Have the tab on the lid directed towards one of the corners of the foil, this helps get enough around and prevents tearing. To help get the bottoms flat I like to grind them down a little on the stovetop, this will help them sit straight in the PC. Place rings in the PC and a piece of folded up foil down if you need more stability. Stack carefully in the PC.





It was suggested by a poster (sorry I can't remember which one) that the hole could be drilled through the side and eliminate the need for the foil and towel. I have tried it and it does work. The tape gets quite wet and must be allowed to dry but it does work fairly well. I do miss not having the towel to write on and it's hard to not touch the tape sometimes so care should be exercised when handling. This would be a good option for those who don't want to use the foil.





Now you just gotta PC them for 40-45 mins. Done.  I like to leave them in the PC overnight to cool, this helps keep the condensation down. Then I when remove them they go straight into my SAB. If you want to keep em around for a while they can be stored safely in the fridge, just don't unwrap em. Remove the foil from them in the SAB and if the tops seem wet, I let them dry in the SAB for a few hours. Leave one of the arm holes open if you do this with a tyvek sleeve stuffed in the hole. Like I said before however, if they feel wet, adjust the way you bring the pressure up in the PC.

Now you have lots of cheap, easy to work with plates that don't need to be ordered and can be put together with stuff you can find around town. You can now safely inoculate any media like grains or LC without the issue of invisible contams. You can also store good cultures for a long time and grow them many times before senescence hits. You can also ward this off by changing your media, this tek ends up making PDA (potato dextrose agar) but it will work just as well with MEA (malt extract agar), PDYA (potato dextrose yeast agar), Dog food agar, grain soak water agar, etc. So don't be afraid to mix it up. Hopefully this lets people dip their toes in the agar pool, trust me its well worth it :awesomenod:



:scarface:

Edit; as more people come on board with this, I hear feedback on ways to use it, substitutions, etc. Here are some relevant links to assist users. I will attempt to update periodically.

The Tiger Drop

Inoculation, transfers, grain inoculation in SAB

Tic Tac Toe (Tiger Drop 2.0)


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OfflineTheApprentice
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Re: Pasty Agar Tek [Re: Pastywhyte] * 3
    #19209034 - 11/30/13 03:29 PM (10 years, 2 months ago)

I love the idea of food coloring!

great write up.

I started my agar adventure on brf paste in a glad mini round lol.

now I love agar!


--------------------


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Invisibleblindingleaf
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Re: Pasty Agar Tek [Re: TheApprentice] * 6
    #19209950 - 11/30/13 07:51 PM (10 years, 2 months ago)

awesome TEK!  I got some personal PM tutoring from pasty about this last week. I have transfers growing on them right now, will post pics as soon as the growth is big enough to see (just a tiny wedge now).  I dunno why, I hate pouring agar, I love doing transfers and stuff, but I dread the pour like I dread going to work.  With this its all ready to go after it cools in the PC!  I can't help but think this will encourage a lot more beginners to start agar.

YO THAT SHIT SO PASTY YO!  (sorry I work overnight at a convenience store in the ghetto, the lingo rubs off occasionally....)

:rofl:


--------------------
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OfflinePsilicon
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Re: Pasty Agar Tek [Re: blindingleaf] * 8
    #19210133 - 11/30/13 08:48 PM (10 years, 2 months ago)

Is this the testimonials section?  I spent a lot of time and effort trying to fuck these up and ended up with a usable agar plate anyway. Props to Pasty for making this excellent tek.


--------------------
Agar - what, why and how?  Everything a beginner needs to know.
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InvisiblePastywhyteMDiscord
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Re: Pasty Agar Tek [Re: Psilicon] * 2
    #19210150 - 11/30/13 08:54 PM (10 years, 2 months ago)

Quote:

TheApprentice said:
I love the idea of food coloring!




Me too, I can't believe more people don't do it :super:

Quote:

blindingleaf said:
I can't help but think this will encourage a lot more beginners to start agar.




I sure hope so :awesomenod:

Quote:

van der griegen said:
I spent a lot of time and effort trying to fuck these up and ended up with a usable agar plate anyway.




Loving it :super:


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Re: Pasty Agar Tek [Re: Pastywhyte] * 2
    #19214047 - 12/01/13 06:14 PM (10 years, 2 months ago)

i like this tek. :thumbup:


--------------------


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OfflineStromriderM
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Re: Pasty Agar Tek [Re: silverstem] * 2
    #19214066 - 12/01/13 06:18 PM (10 years, 2 months ago)

:rockon:


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Invisibleteknix
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Re: Pasty Agar Tek [Re: Pastywhyte] * 3
    #19214089 - 12/01/13 06:22 PM (10 years, 2 months ago)

It seems better to just use a jar if you wanna no pour.


"Study: Most Plastics Leach Hormone-Like Chemicals."

Study: Most Plastics Leach Hormone-Like Chemicals. Makers of water bottles, including Camelback, now sell products that don't contain BPA, a chemical that can mimic the sex hormone estrogen. But a new study says that even if they don't contain BPA, most plastic products release estrogenic chemicals. Mar 2, 2011

http://www.npr.org/2011/03/02/134196209/study-most-plastics-leach-hormone-like-chemicals

"As scientists become more aware of the chemicals that leech out of plastics and make their way into our bodies, they are uncovering a variety of health issues that result. This includes cancer, reproductive issues, immune system suppression and problems with childhood development just to name a few."

http://www.onegreenplanet.org/environment/plastic-toxins-are-leaching-into-your-food-and-water/

Another thing to consider is the outgassing of most plastics;

"Outgassing can be significant if it collects in a closed environment where air is stagnant or recirculated."

https://en.wikipedia.org/wiki/Outgassing

"Most plastics are made by a process called "free-radical polymerisation" in which small molecules are joined together into long chains. Although the small molecules (things like styrene and vinyl chloride) are highly toxic, the long molecules made by joining hundreds or thousands of them together (polystyrene and polyvinylchloride (PVC)) are non-toxic. At high enough temperatures, however, the free-radical polymerisation reaction goes in the opposite direction; instead of small molecules joining together to make big ones, the big ones break down to give off the poisonous small ones."

http://www.madsci.org/posts/archives/2001-01/978994422.Ch.r.html


Edited by teknix (10/03/17 10:51 PM)


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Invisibleteknix
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Re: Pasty Agar Tek [Re: teknix] * 2
    #19214149 - 12/01/13 06:35 PM (10 years, 2 months ago)

It also seems like it might be a bit difficult to observe the growth through the lid and detect any contaminates readily and early on.


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InvisiblePastywhyteMDiscord
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Re: Pasty Agar Tek [Re: teknix] * 3
    #19214182 - 12/01/13 06:44 PM (10 years, 2 months ago)

Quote:

teknix said:
Isn't it cheap to by a petri dish?



Sure if you don't count $20 in shipping, that's a lot to some people.

Quote:

teknix said:
I think you might have a problem with settling of the nutrients at the bottom of the container.



Nope, that's why we heat it up to dissolve most of the nutes. This and many other no pour teks take advantage of this. Fact is that if the nutes were not soluble, LC wouldn't work either.

Quote:

teknix said:
Do you find that you have bubbles in the dishes after you're done?



Never happened yet.

Quote:

teknix said:
It also seems like it might be a bit difficult to observe the growth through the lid and detect any contaminates readily and early on.



Nope I look through the sides. Quite easy to spot satellite colonies and bacteria especially against the colored agar, which makes them stand out.

Quote:

teknix said:
It's much more efficient, imo, to just buy some premade PDA, at put it in one jar so you don't waste all the room in your PC.




Why not just do it in your oven? I'm not even going to debate this with you here. It states at the top of the thread that this is for people who don't want to be ordering shit online, or paying high prices for stuff they could do for pennies. I'm sure that you can appreciate people not wanting to order suspicious items online to their homes given your reluctance to post a single pic of anything you claim to have done.


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OfflineYuri.Pono
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Re: Pasty Agar Tek [Re: teknix] * 2
    #19214188 - 12/01/13 06:46 PM (10 years, 2 months ago)

how many times have you used this tek with them same type of glad jars. just wondering how many uses til the warp & are useless?


--------------------
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InvisiblePastywhyteMDiscord
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Re: Pasty Agar Tek [Re: Yuri.Pono] * 2
    #19214209 - 12/01/13 06:51 PM (10 years, 2 months ago)

Quote:

Yuri.Pono said:
how many times have you used this tek with them same type of glad jars. just wondering how many uses til the warp & are useless?



I have done as many as 10 runs with the same containers. Sometimes I get a bad batch that don't last as long. I'd say an average of 5 runs would be fair. That works out to 40 plates for $4. Since I started with these I could not say how many times I have done these, but I am doing about 25-30 a month.


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Invisibleteknix
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Re: Pasty Agar Tek [Re: Pastywhyte] * 2
    #19214219 - 12/01/13 06:53 PM (10 years, 2 months ago)

Quote:

Pastywhyte said:
I'm not even going to debate this with you here. It states at the top of the




Good, I never said anything about it. . .


People charge you $20 for shipping? Free shipping if you order enough stuff from amazon. 5 bucks for 20 of them, how much do you pay for your little containers?

http://www.amazon.com/Karter-Scientific-Plastic-60x15mm-Sterile/dp/B005Z4QU5U/ref=sr_1_1?ie=UTF8&qid=1385949104&sr=8-1&keywords=petri+dish

These ones are twice as much, which is half the amount for the same price:

http://www.amazon.com/Sure-Fresh-Storage-Containers-10-ct/dp/B006USXTSG/ref=sr_1_4?ie=UTF8&qid=1385949169&sr=8-4&keywords=small+container


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Invisibleteknix
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Re: Pasty Agar Tek [Re: Pastywhyte] * 2
    #19214221 - 12/01/13 06:54 PM (10 years, 2 months ago)

Quote:

Pastywhyte said:
Quote:

Yuri.Pono said:
how many times have you used this tek with them same type of glad jars. just wondering how many uses til the warp & are useless?



I have done as many as 10 runs with the same containers. Sometimes I get a bad batch that don't last as long. I'd say an average of 5 runs would be fair. That works out to 40 plates for $4. Since I started with these I could not say how many times I have done these, but I am doing about 25-30 a month.




Where did you buy those at?


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InvisiblePastywhyteMDiscord
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Re: Pasty Agar Tek [Re: teknix] * 3
    #19214228 - 12/01/13 06:57 PM (10 years, 2 months ago)

The grocery store. $4 is the high price. A couple months ago they had them on sale for $2.50 and I loaded up. Might even be cheaper in the U.S. We seem to get hosed on prices for everything up here.


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Invisibleteknix
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Re: Pasty Agar Tek [Re: Pastywhyte] * 2
    #19214245 - 12/01/13 07:01 PM (10 years, 2 months ago)

What grocery store and For how many of them?

I guess if you can reuse them you might save a bit, but I hate cleaning plastic that has been melted with agar and nutes stuck in the sides.

I used to use pyrex because I thought it would be cheaper in the long run, but really it seems more of a hassle when I could do better things that clean up all the dishes, plus those would take up quite a bit more space. Space is money too.


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Re: Pasty Agar Tek [Re: teknix] * 2
    #19214308 - 12/01/13 07:16 PM (10 years, 2 months ago)

Nice tek and write up. Well documented  - with photos. Imagine that...

Thanks


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InvisiblePastywhyteMDiscord
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Re: Pasty Agar Tek [Re: budkatz] * 3
    #19214359 - 12/01/13 07:31 PM (10 years, 2 months ago)

I've seen them in every grocery store I have been in. They are not hard to find. Truth is they are not hard to clean. Soak em for 5 min in soapy water, then a quick wipe with a dishcloth. Done. I can wash up 30 in about 7 min including the 5 min soak time. Now it's even easier cause I just get my wife to do it :lol:

Quote:

budkatz said:
Nice tek and write up. Well documented  - with photos. Imagine that...

Thanks




Appreciate it man, good vibes :super:


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InvisibleViolet
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Re: Pasty Agar Tek [Re: Pastywhyte] * 2
    #19214426 - 12/01/13 07:48 PM (10 years, 2 months ago)

Nice, Pasty!

Quote:

Pastywhyte said:
Quote:

Yuri.Pono said:
how many times have you used this tek with them same type of glad jars. just wondering how many uses til the warp & are useless?



I have done as many as 10 runs with the same containers. Sometimes I get a bad batch that don't last as long. I'd say an average of 5 runs would be fair. That works out to 40 plates for $4. Since I started with these I could not say how many times I have done these, but I am doing about 25-30 a month.



Those should last you far more than 5 uses.
Mine are screw-top of course but I am quite sure that many of mine have had more than 50 runs in the cooker.  All of them have certainly had more than 10 runs.
Only visible wear is scratching from using scrubbies a couple times to clean them out.

Also I never get a "bad batch" of the screw-top ones, at least not with zip-locs or the most similar generics (the purple/blue lid kind they re-label for different marts)

I'll add, screw-top containers don't need lid modifications!


--------------------
Intentionally or not, here in mushcult we are purveyors of love culture and enlightenment movement. Let's try to act like it!

PODS TEK - Growing Invitro with BRF/verm or Grass Seed containers
The simplest, quickest, safest tek!  For beginners, culturers, lazy people, stealth lovers, contam haters, and alternative seekers!
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InvisiblePastywhyteMDiscord
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Re: Pasty Agar Tek [Re: Violet] * 2
    #19214509 - 12/01/13 08:10 PM (10 years, 2 months ago)

Thanks Violet. A few things on your well founded comments.

Quote:

Violet said:
Those should last you far more than 5 uses.



For the most part they do, I was just trying to compensate for the occasional bad batch which I have run across, driving the average down. Fact is that I still have some of the very first ones I ever did and have probably gone well over 10 runs, just trying to be on the safe side of things.

Quote:

Violet said:
Mine are screw-top of course but I am quite sure that many of mine have had more than 50 runs in the cooker.  All of them have certainly had more than 10 runs.



No question. I have used your screw top ziplocks many times back when I was first experimenting with no pour well before I had an account here. My reasons for not using them is ergonomic rather than durability. I find dealing with the large size of the container and the screw lid cumbersome. I can hold the mini round and crack the lid with one hand allowing the other hand free to use my tools and exposing very little of the container to the air in the SAB. Plus I can fit many more mini rounds in my fridge which is a plus when you get over 50 cultures on the go.

Your ziplocks work perfectly fine for most purposes. I did successful agar with them before I ever saw your tek. It all comes down to personal preference. For me ergonomics and compact size trump durability and not needing to modify the lids :super:


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Re: Pasty Agar Tek [Re: Pastywhyte] * 3
    #19214623 - 12/01/13 08:33 PM (10 years, 2 months ago)

Nice write up man, I'm all about no-pour. :thumbup:

I use those little flat ball jars cause mine get all beat up and shit spilled on them in the fridge (live with 4 other people), but I might have to give these a shot some time, or the screw top ones.  I like your point about the one handed opening, that could be nice in some circumstances, for sure.


--------------------
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-------------------

Have some medicinal mushrooms and want to get the most out of them?  Try this double extraction method.


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Re: Pasty Agar Tek [Re: Forrester] * 2
    #19214656 - 12/01/13 08:41 PM (10 years, 2 months ago)

This is awesome!  Thank you!

Cue anne halonium telling us this is too 'oliver-twist' for any serious grower.


--------------------
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Re: Pasty Agar Tek [Re: elasticaltiger] * 1
    #19214784 - 12/01/13 09:13 PM (10 years, 2 months ago)

Quote:

Forrester said:
Nice write up man, I'm all about no-pour. :thumbup:



Nice to see serious people who do no pour, I welcome your feedback!

Quote:

elasticaltiger said:
This is awesome!  Thank you!



Very welcome, thanks for reading :thumbup:


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Re: Pasty Agar Tek [Re: teknix] * 1
    #19215311 - 12/01/13 11:48 PM (10 years, 2 months ago)

Quote:

teknix said:
Which is why people don't pre-make their dishes or slants to PC.




People do pre-make slants before PCing. You just give it a shake when you set it out to cool.

Mephisto
Myself

Dishes are a different story.


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Re: Pasty Agar Tek [Re: FrankHorrigan] * 1
    #19215376 - 12/02/13 12:04 AM (10 years, 2 months ago)

how do these not melt??? :confused:


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Re: Pasty Agar Tek [Re: tripp23] * 3
    #19215443 - 12/02/13 12:25 AM (10 years, 2 months ago)

Quote:

FrankHorrigan said:
People do pre-make slants before PCing. You just give it a shake when you set it out to cool.

Dishes are a different story.




C'mon Frank, I knew you would have no love for these but, there has been a lot of successful no pour done by a lot of people :wink: Is the tek the final word on agar? Not at all. I do pour agar, and there are limits to no pour as well that I do recognize. However for your average joe starting out, this is pretty forgiving and hard to fuck up. I like to think of it as agar that's as easy as pf tek :lol:

Quote:

tripp23 said:
how do these not melt??? :confused:



PP5 code plastic will not melt in the pc. Wouldn't recommend any other type of plastic for this tho


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Re: Pasty Agar Tek [Re: Pastywhyte] * 2
    #19215455 - 12/02/13 12:30 AM (10 years, 2 months ago)

Actually you misunderstood me, I was saying that you cannot pre-make petri dishes and then PC them, be them glass or plastic or whatever. 

No pour dishes are fine, it's good for beginners too. I wasn't knocking it :shrug:


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Re: Pasty Agar Tek [Re: FrankHorrigan] * 1
    #19215536 - 12/02/13 01:00 AM (10 years, 2 months ago)

Sorry I completely misunderstood you. I guess I misjudged your stance on no pour.
:kenthumbup:


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Re: Pasty Agar Tek [Re: Pastywhyte] * 1
    #19215554 - 12/02/13 01:04 AM (10 years, 2 months ago)

You would :lol:


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Re: Pasty Agar Tek [Re: FrankHorrigan] * 1
    #19216047 - 12/02/13 06:30 AM (10 years, 2 months ago)

:ohyou:


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Re: Pasty Agar Tek [Re: Pastywhyte] * 1
    #19217210 - 12/02/13 01:04 PM (10 years, 2 months ago)

:manofapproval:
nice stuff broski!


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Re: Pasty Agar Tek [Re: cronicr] * 1
    #19217970 - 12/02/13 04:10 PM (10 years, 2 months ago)

is it me or does this growth look....pasty  :smile:





thx again for the tek man...its obviously working


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Re: Pasty Agar Tek [Re: blindingleaf] * 1
    #19218694 - 12/02/13 06:41 PM (10 years, 2 months ago)

Nice lookin plate blinding! Got yerself a good looking culture there :awesomenod:


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Re: Pasty Agar Tek [Re: blindingleaf] * 1
    #19219401 - 12/02/13 08:52 PM (10 years, 2 months ago)

Quote:

blindingleaf said:
is it me or does this growth look....pasty  :smile:





thx again for the tek man...its obviously working




:happyclaps: going to try this tomorrow along with Violet's agar tek.

Since these aren't technically 'plates' what should we call them? Saying 'hey man your PP5 food container looks awesome!' doesn't roll off the tongue so easy.  :lol:


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Re: Pasty Agar Tek [Re: elasticaltiger] * 1
    #19219433 - 12/02/13 08:59 PM (10 years, 2 months ago)

Mini-round agars? Pasty agars?


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Re: Pasty Agar Tek [Re: Psilicon] * 1
    #19219730 - 12/02/13 10:03 PM (10 years, 2 months ago)

Quote:

van der griegen said:
Mini-round agars? Pasty agars?




I like both of those

:kenthumbup:


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Re: Pasty Agar Tek [Re: Pastywhyte] * 2
    #19219822 - 12/02/13 10:20 PM (10 years, 2 months ago)

How about Pasty Plates?

Or Pasty Puddles? That holds true to the 'PP' in 'PP5' Someday a n00b will ask 'what does the PP in PP5 stand for?' and well say 'it's pasty-puddles duh use the search function'


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Re: Pasty Agar Tek [Re: elasticaltiger] * 1
    #19219906 - 12/02/13 10:35 PM (10 years, 2 months ago)

Quote:

elasticaltiger said:
How about Pasty Plates?

Or Pasty Puddles? That holds true to the 'PP' in 'PP5' Someday a n00b will ask 'what does the PP in PP5 stand for?' and well say 'it's pasty-puddles duh use the search function'




Damn I like the sound of Pasty Plates for sure :super: Pasty puddles sounds cool too but I don't want people to think were meltin meth here :lmafo:


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Re: Pasty Agar Tek [Re: elasticaltiger] * 2
    #19220003 - 12/02/13 10:54 PM (10 years, 2 months ago)

Everyone is trying to name things after themselves these days, it's sort of a silly ego trip if you ask me.

It's not the "FH grain dunk" or the "Citric polyfill tek". It's just the "grain dunk" or "dialed-in monotubs."

Best to name things descriptively so people know what they are at a glance :shrug:


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Re: Pasty Agar Tek [Re: FrankHorrigan] * 1
    #19220024 - 12/02/13 11:01 PM (10 years, 2 months ago)

Your probably right, look at all the hell Damion's 50/50 tek has caused :lmafo: If that was the case I would probably want to call em something like "Easy Plates" something that seems noob friendly :thumbup:


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Re: Pasty Agar Tek [Re: Pastywhyte]
    #19220032 - 12/02/13 11:02 PM (10 years, 2 months ago)

"Easy agar mini-rounds" sounds about right...maybe something like "all the benefits of agar with none of the hassle of petri dishes" :wink:

And if it ends up being reviled in a year, at least your name isn't all over it :lol:


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Re: Pasty Agar Tek [Re: FrankHorrigan] * 1
    #19220181 - 12/02/13 11:53 PM (10 years, 2 months ago)

Quote:

FrankHorrigan said:
Everyone is trying to name things after themselves these days, it's sort of a silly ego trip if you ask me.

It's not the "FH grain dunk" or the "Citric polyfill tek". It's just the "grain dunk" or "dialed-in monotubs."

Best to name things descriptively so people know what they are at a glance :shrug:




When I'm talking with my biology friends down at the university we always say 'franks proper pasteurization' and my incubator will always be 'franks titi' to me and when I come home, every night I gently slide my hand down the edges of Frank's Titi and slowly lift up its top to inspect its firm warmth. After my inspection I let the top back down on to it's hard edges and whisper gently 'good girl'

BUUUUUUUUUT then again I'm just going to call them 'easy plates' the next time a new shroomerite asks about the dangers of pouring agar in an SAB.


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Afraid to Start Growing From Your Own Prints? Drop it Like a Tiger!
No Pouring. No Syringes. No Cutting. No flaming. No Contamination. No Bullshit.

"The best thing to do while your waiting is to start more stuff. I usually got so much happening that I have tossed projects simply because I didn't have time for them. -Pastywhite QFT

Pastywhite's Easy Agar Tek (PastyPlates)

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Re: Pasty Agar Tek [Re: elasticaltiger] * 1
    #19220185 - 12/02/13 11:55 PM (10 years, 2 months ago)

:whateveryousayfreak:


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Re: Pasty Agar Tek [Re: FrankHorrigan]
    #19220197 - 12/03/13 12:02 AM (10 years, 2 months ago)

Quote:

FrankHorrigan said:
:whateveryousayfreak:




You should be so honored! My ex girlfriends don't even get such treatment!


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Pastywhite's Easy Agar Tek (PastyPlates)

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Re: Pasty Agar Tek [Re: elasticaltiger]
    #19220209 - 12/03/13 12:05 AM (10 years, 2 months ago)

Well the edges of frank's Titi are reserved for his ladies, so back off :hehehe:


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Re: Pasty Agar Tek [Re: elasticaltiger]
    #19220220 - 12/03/13 12:08 AM (10 years, 2 months ago)

Quote:

elasticaltiger said:
BUUUUUUUUUT then again I'm just going to call them 'easy plates' the next time a new shroomerite asks about the dangers of pouring agar in an SAB.




I'm good with that. Really I wrote this for the community so, whatever is good for them is fine by me. Easy plates has a nice ring IMO and gets the message across, so if that's what catches on then :thumbup:


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Re: Pasty Agar Tek [Re: Pastywhyte]
    #19220231 - 12/03/13 12:12 AM (10 years, 2 months ago)

Quote:

elasticaltiger said:
the next time a new shroomerite asks about the dangers of pouring agar in an SAB.




You should tell them that there are none...because there are none.


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Re: Pasty Agar Tek [Re: FrankHorrigan]
    #19220246 - 12/03/13 12:16 AM (10 years, 2 months ago)

Can't stop thinking about Franks warm titi :lmafo:


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Re: Pasty Agar Tek [Re: Stromrider]
    #19254820 - 12/10/13 11:00 AM (10 years, 2 months ago)

Pasty, about to do this tek for some xfers.

I normally don't add a hole for GE when it comes to my agar jars.

do I need to add them to the pp5?


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Re: Pasty Agar Tek [Re: TheApprentice]
    #19254837 - 12/10/13 11:06 AM (10 years, 2 months ago)

if you got the twist tops it's not needed but for anything that snaps on i use one


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Re: Pasty Agar Tek [Re: cronicr]
    #19254843 - 12/10/13 11:07 AM (10 years, 2 months ago)

Got it.  About to cut holes then


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Re: Pasty Agar Tek [Re: TheApprentice]
    #19254940 - 12/10/13 11:35 AM (10 years, 2 months ago)

The hole helps keep the vacuum from crushing the container. I found that if I use too small of a hole it will buckel a bit so that's why I recommended the 1/4". Have had some good feedback on the tek and will be testing some alternatives to micropore tape as some people have a hard time locating it as well. Will have my findings up soon.


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Re: Pasty Agar Tek [Re: Pastywhyte]
    #19254967 - 12/10/13 11:41 AM (10 years, 2 months ago)

Well I am using polyfil.  About 1/4 hole I think.  I'll post back results in a few.  Cookin up some less nutes in my agar for rhizo growth!

yay!


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Re: Pasty Agar Tek [Re: TheApprentice]
    #19254982 - 12/10/13 11:44 AM (10 years, 2 months ago)

Should work, let us know how the polyfill treats you.


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Re: Pasty Agar Tek [Re: Pastywhyte]
    #19254989 - 12/10/13 11:45 AM (10 years, 2 months ago)

i use polly, works fine:thumbup:


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Re: Pasty Agar Tek [Re: cronicr]
    #19255028 - 12/10/13 11:53 AM (10 years, 2 months ago)

Quote:

cronicr said:
i use polly, works fine:thumbup:



:themoreyouknow:


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Re: Pasty Agar Tek [Re: Pastywhyte]
    #19262035 - 12/11/13 07:04 PM (10 years, 2 months ago)

:hi:


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Re: Pasty Agar Tek [Re: cronicr] * 1
    #19262957 - 12/11/13 09:37 PM (10 years, 2 months ago)

They turned out fine.  A little condensation in the jars, but I didn't use foil when I pc'd.

its a recession.  Don't judge me.

haven't done any xfers yet.  Out of disposable gloves.  Keep forgetting to steal then from work.


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Re: Pasty Agar Tek [Re: TheApprentice] * 1
    #19263021 - 12/11/13 09:46 PM (10 years, 2 months ago)

I ain't gonna judge, just glad people are getting on board :thumbup: agar is too good to let excuses like "its hard " or "I don't want to have to order stuff online" to prevent people from trying this exciting aspect of the hobby.


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Re: Pasty Agar Tek [Re: Pastywhyte]
    #19264218 - 12/12/13 03:50 AM (10 years, 2 months ago)

question:
if these plates are "poured" in larger quantities (say 3 TBS instead of 1 TBS per plate) would they make for longer term storage in the fridge for cultures because of the increased depth (wrapped in plastic/bagged up of course)?  I do not have $ for slants right now, or will I in the next 2 months.  I have some untested cube isolates, as well as maitaki and skiitaki clones taken from grocery store that grew out clean, all of them r on these pasty plates, and time restrictions r forcing me to delay the starts of newer grow projects.  how long do u think these could last in fridge with a larger amount of agar...and would it be beneficial to tilt them a bit, like with a slant, while cooling if this was to be done?  I have had this happen inadvertently while they cooled in the pc, and those plates are a bitch to transfer from because the agar is so deep in the middle of the culture, but it got me thinking about the relationship to the agars depth and its shelf life.

Im going to have to do it either way but I wanted to throw it out there for opinions.

also might as well ask...what is the longest any of you has kept a petri/easy plate/no pour plate WITH growth in the fridge and it still remain viable?  I personally have used colonized grain jars after 5 months with success, but have never used (or tried) agar from fridge that was longer than a month.
(Pasty, I can move this to a new thread if u want, its not meant to thread jack, but I put it here because ur plates can be poured to a larger depth than petris, so i thought it was related enough to make post here, and if it works, u can add the info @ the end of ur tek? :shrug: )


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Re: Pasty Agar Tek [Re: blindingleaf]
    #19264472 - 12/12/13 06:35 AM (10 years, 2 months ago)

I'm gonna say that if you made it a little thicker like say 3/8", it would probably be good for at least 3 months. No need to cool it at an angle, the reason that is done for slants is to provide a larger growing surface. I have cannot say exactly how I i have kept and successful transferred from a plate but it was older than 3 months for sure.


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Re: Pasty Agar Tek [Re: Pastywhyte]
    #19315878 - 12/23/13 12:02 PM (10 years, 2 months ago)

gona give this a run tonight, opened my mail box today and found 6 prints some PE swabs and 2 pounds of agar powder, now x-mas is gonna feel stupidly pointless but today kicks ass:thumbup:


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Re: Pasty Agar Tek [Re: Pastywhyte]
    #19316141 - 12/23/13 01:23 PM (10 years, 2 months ago)

Quote:

Pastywhyte said:
glad people are getting on board :thumbup: agar is too good to let excuses like "its hard " or "I don't want to have to order stuff online" to prevent people from trying this exciting aspect of the hobby.



Agar is eazy with the no pour method.  The most important thing to stress is that there is most likely something in your house right now that can be part of an agar mix.  It has been pointed out time and time again, no pour is for the study by an individual who does not want to pour or order real plates (Cost/paranoia/both?). 


I have tried both the pp5 and the glass 1/2 pint jars back and forth, and prefer the glass jars.  I have been getting no condensation at all, and I can do 12 with just a bit over 250ml media.  I tried the quarter pint small mouth rounds.  What a pain in the ass....all of mine are "quilted" on the outside so I have zero chance of seeing anything from the side.  They are too small on the bottom, and require more monitoring or else will cover the plate pretty quick.  1/2 pint plastic lids are my sweet spot....again, just my opinion.

Plastic vs glass vs size is preferential.  The cat is still skinned. 

This is a great place to start when people ask "What is an eazy method to agar?"


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Re: Pasty Agar Tek [Re: HypnotoadCroaked]
    #19316151 - 12/23/13 01:26 PM (10 years, 2 months ago)

i prefer all the above and then some, if you can fill it up and pc it....get to work


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Re: Pasty Agar Tek [Re: cronicr]
    #19316263 - 12/23/13 01:50 PM (10 years, 2 months ago)

hey whippy when you decide to try the agave let me know how it works out for ya, i have it in my house but never thought to try it
edit: nm i foun some of your posts alreay:thumbup:


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Edited by cronicr (12/23/13 01:55 PM)


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Re: Pasty Agar Tek [Re: cronicr]
    #19316310 - 12/23/13 01:57 PM (10 years, 2 months ago)

Quote:

cronicr said:
hey whippy when you decide to try the agave let me know how it works out for ya, i have it in my house but never thought to try it
edit: nm i foun some of your posts alreay:thumbup:



I think that the agave is similar to honey.  It is more than a simple sugar.  I have not tried agave + agar without potato, but I think it would work great in the correct formulation.


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Re: Pasty Agar Tek [Re: HypnotoadCroaked]
    #19316319 - 12/23/13 01:59 PM (10 years, 2 months ago)

Quote:

Whippy said:
This is a great place to start when people ask "What is an eazy method to agar?"




That's all I was hoping to do. Show how easy it can be, and if some people prefer to do 1/2 pint jars after a few kicks at the can that's cool. The point is giving it a try and seeing how easy it really is.


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Re: Pasty Agar Tek [Re: Pastywhyte]
    #19342971 - 12/29/13 06:30 PM (10 years, 1 month ago)

So I was at Walmart today, the 2nd one I've tried and it appears they no longer carry my beloved little square flat Ball jars :sad:

So I was like alright, what would Pasty do?

Bam! :lol:



I couldn't remember what kind of tupperware it was you used, but it looks like I ended up getting the exact same ones.  I didn't have any micropore tape so I siliconed in some tyvek.  We'll see if it worked!


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Re: Pasty Agar Tek [Re: Forrester]
    #19342975 - 12/29/13 06:32 PM (10 years, 1 month ago)

:headbang3:
i used the twist tops


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Re: Pasty Agar Tek [Re: cronicr]
    #19342982 - 12/29/13 06:34 PM (10 years, 1 month ago)

^^^ shitty ass Walmart here didn't have those or I would have too...


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Re: Pasty Agar Tek [Re: Forrester]
    #19342999 - 12/29/13 06:39 PM (10 years, 1 month ago)

I really hate the pop tops tho.

would have loved to get the twist opens


Forrester, try your local jewel or albertsons grocery


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Re: Pasty Agar Tek [Re: TheApprentice]
    #19343014 - 12/29/13 06:42 PM (10 years, 1 month ago)

i found thes things called baby cubes that i'm using for germination that i like
they sanp nicely into a little stand they come with and i can just put the whole thing on it's side while i work:thumbup: there about five bux in the babyfood section of most retail stores


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Re: Pasty Agar Tek [Re: TheApprentice]
    #19343015 - 12/29/13 06:43 PM (10 years, 1 month ago)

Twist-top forever :raver2:


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Re: Pasty Agar Tek [Re: Violet]
    #19343020 - 12/29/13 06:45 PM (10 years, 1 month ago)

i had a stck of 3 dollar off coupons but i lost them, i was getting the stacks of 3 for about 50 cents in the summer time:smbfacepalm:


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Re: Pasty Agar Tek [Re: cronicr]
    #19343030 - 12/29/13 06:48 PM (10 years, 1 month ago)

I've never seen Ziplocs for less than $2.25 on sale for 3 pints.
Other brands yeah, but they're still not even worth it


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Re: Pasty Agar Tek [Re: TheApprentice]
    #19343039 - 12/29/13 06:51 PM (10 years, 1 month ago)

Quote:

TheApprentice said:
Forrester, try your local jewel or albertsons grocery




Jewel?  Albertsons?  You speak a foreign language!


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Re: Pasty Agar Tek [Re: Violet]
    #19343116 - 12/29/13 07:11 PM (10 years, 1 month ago)

Quote:

Violet said:
I've never seen Ziplocs for less than $2.25 on sale for 3 pints.
Other brands yeah, but they're still not even worth it



there like 4.99 average at most the stores around here they were 3.50 for the most of summer:headbang3:


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Re: Pasty Agar Tek [Re: Forrester]
    #19343125 - 12/29/13 07:13 PM (10 years, 1 month ago)

Quote:

Forrester said:
So I was at Walmart today, the 2nd one I've tried and it appears they no longer carry my beloved little square flat Ball jars :sad:

So I was like alright, what would Pasty do?

Bam! :lol:



I couldn't remember what kind of tupperware it was you used, but it looks like I ended up getting the exact same ones.  I didn't have any micropore tape so I siliconed in some tyvek.  We'll see if it worked!



Looks good man :thumbup:


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Re: Pasty Agar Tek [Re: Pastywhyte]
    #19345102 - 12/30/13 05:49 AM (10 years, 1 month ago)

the shop n bag 2 blocks from me in the city of brotherly love has the them both, twist and pop tops.  i think they (shop n bag) r franchised though, so it may be up to the owner of the store what to carry.


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Re: Pasty Agar Tek [Re: blindingleaf]
    #19351289 - 12/31/13 02:06 PM (10 years, 1 month ago)

pasty i blame u for having too many plates to work with.



gonna have to dump some.  no room in fridge and all jars are in use, so i can't noc up more grain yet.  many of these have grown to the edge, which i haven't had a problem with yet taking wedges up against the walls, but with regular petri i almost always get a bacteria contam if i take a wedge from the edge.  haha sounds like a good thread name "Wedge from the Edge"
what is ur opinion on taking a wedge from the edge of these PP5's vs a regular petri?  am i just lucky or are the higher walls offering a better barrier?


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Re: Pasty Agar Tek [Re: blindingleaf]
    #19351449 - 12/31/13 02:55 PM (10 years, 1 month ago)

Quote:

blindingleaf said:
pasty i blame u for having too many plates to work with.






:lolsy: Welcome to my world :lol:

Quote:

blindingleaf said:
what is ur opinion on taking a wedge from the edge of these PP5's vs a regular petri?  am i just lucky or are the higher walls offering a better barrier?




I have done it and not experienced signigificant issues, not sure if I was just lucky or what. Most bacteria spreads through contact and I find the little handle on the lids helps keep me from touching the lids too close to where they close. This might be the reason for my luck with transfers near the edge :shrug: Its probably not the best practice but its not certain doom if you do.


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Re: Pasty Agar Tek [Re: Pastywhyte]
    #19351631 - 12/31/13 03:44 PM (10 years, 1 month ago)

I have been quite surprised with how little "edge" contamination I have had.  The only times I have seen bacteria around edges is when I was working with OD prints and clone flesh.  I have yet to see any random "Hey I did not put that there".  I dunno what I am doing right, but thus far agar has been a god-send.  I have had no issues letting the mycelium poke up the sides, and simply just rubbing a few "feather ends" and poking them into another dish. 

Mileage may vary.


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Re: Pasty Agar Tek [Re: HypnotoadCroaked]
    #19351661 - 12/31/13 03:52 PM (10 years, 1 month ago)

yea i seem to have better luck with agar work than g2g.  none of my wedge to grain jars contam ever, but one in 15 jars of g2g will contam.  i am using postal tyvek on these, one layer over the lid, secured down by rim, and have done this with success before.  i think i need a taller SAB because many times, the last of the master jars grain will be difficult to shake in the last g2 jar without jostling around a lot in the say, and letting the two jar rims touch. can't wait to get electric drill to make the plastic lids.  i have sfd's but only six, so when they are in use, i go tyvek until i can turn those six into 36


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Re: Pasty Agar Tek [Re: HypnotoadCroaked]
    #19351675 - 12/31/13 03:57 PM (10 years, 1 month ago)

this thread is awesome. I never even new about the twist top PP5s before. they are way better to work with than the pop tops.
EDIT I no longer prefer screw tops I posted this back before I knew what the fuck I was doing.


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Edited by MudaFuka (08/23/14 12:27 AM)


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Re: Pasty Agar Tek [Re: MudaFuka]
    #19351686 - 12/31/13 04:00 PM (10 years, 1 month ago)



i've been using these lately, just to germinate for the most part but there nice and small and i don't waiste alot of agar like i feel i do with the big twist tops


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Re: Pasty Agar Tek [Re: cronicr]
    #19351842 - 12/31/13 04:45 PM (10 years, 1 month ago)

Quote:

cronicr said:


i've been using these lately, just to germinate for the most part but there nice and small and i don't waiste alot of agar like i feel i do with the big twist tops



do you have to add any holes to those things for GE


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Re: Pasty Agar Tek [Re: MudaFuka]
    #19351858 - 12/31/13 04:49 PM (10 years, 1 month ago)

yep theres a hole with poly there:thumbup:


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Re: Pasty Agar Tek [Re: cronicr]
    #19351863 - 12/31/13 04:50 PM (10 years, 1 month ago)

and pasty i'm gonna try get this added to the info section if you don't mind:thumbup:
gathering links up right now for some updated teks


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Re: Pasty Agar Tek [Re: cronicr]
    #19351916 - 12/31/13 05:02 PM (10 years, 1 month ago)

Quote:

cronicr said:
and pasty i'm gonna try get this added to the info section if you don't mind:thumbup:
gathering links up right now for some updated teks



Not at all, more exposure = more people doing agar :awesomenod:


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Re: Pasty Agar Tek [Re: cronicr]
    #19352715 - 12/31/13 08:37 PM (10 years, 1 month ago)

Quote:

cronicr said:


i've been using these lately, just to germinate for the most part but there nice and small and i don't waiste alot of agar like i feel i do with the big twist tops




20-25mL, just like petris...
500mL still makes 20+


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Re: Pasty Agar Tek [Re: Violet]
    #19352719 - 12/31/13 08:40 PM (10 years, 1 month ago)

yes i could have said the twist tops and petris and or any of them lol, i like the tiny ones to start on, good watching out violet and happy new years:thumbup:


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Re: Pasty Agar Tek [Re: cronicr]
    #19352726 - 12/31/13 08:41 PM (10 years, 1 month ago)

Happy new year chron, pasty, everyone!


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Re: Pasty Agar Tek [Re: Violet]
    #19353126 - 12/31/13 10:57 PM (10 years, 1 month ago)

:rockon: :headbang3: :rockon:


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Re: Pasty Agar Tek [Re: Pastywhyte] * 2
    #19354743 - 01/01/14 01:30 PM (10 years, 1 month ago)

Quote:

Pastywhyte said:
Finally I spoon in the agar mix into my glad mini rounds. I like to just give them a wipe with a damp paper towel first to clear out any dust or debris. No need to be spraying them with alcohol or nothing. Place the lids on the top and close. Finally I like to fold up a square of paper towel and place on top. Condensation can be a bit of an issue and you don't want to get the micro pore tape too wet. The paper towel helps with this. I have found as well that the paper towel will be too wet if you bring the pressure up to fast in the PC, which will lead to the agar boiling up on the sides. This is a great indication of how good your technique is for this. Here is the agar in the PP5's and how I do the paper towel.




hey bro you should try letting your agar get to room temp and firm before putting your lid on and loading, helps a bunch with the condensation, i barely ever get any:thumbup:


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Edited by cronicr (01/01/14 01:37 PM)


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Re: Pasty Agar Tek [Re: cronicr]
    #19354845 - 01/01/14 01:59 PM (10 years, 1 month ago)

Quote:

cronicr said:
hey bro you should try letting your agar get to room temp and firm before putting your lid on and loading, helps a bunch with the condensation, i barely ever get any:thumbup:




No shit?  I gotta try that.  So you heat it up to dissolve everything in, then let cool, then PC?


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Re: Pasty Agar Tek [Re: Forrester] * 1
    #19354852 - 01/01/14 02:01 PM (10 years, 1 month ago)

yep:thumbup:


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Re: Pasty Agar Tek [Re: cronicr]
    #19354855 - 01/01/14 02:02 PM (10 years, 1 month ago)


i've been getting none with my spice jars and very little/none on my pp5's


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Re: Pasty Agar Tek [Re: cronicr]
    #19355110 - 01/01/14 03:27 PM (10 years, 1 month ago)

Nice!  I'll give it a try next time I do mine :cool:


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Re: Pasty Agar Tek [Re: cronicr] * 1
    #19355333 - 01/01/14 04:27 PM (10 years, 1 month ago)

Quote:

cronicr said:
hey bro you should try letting your agar get to room temp and firm before putting your lid on and loading, helps a bunch with the condensation, i barely ever get any:thumbup:




I totally agree, I actually do this already, I guess that was a little something that got overlooked in the writeup and will be adjusted, thanks cron :super:

Actually I will be making a few revisions/additions in a little bit. An alternative to micropore tape has been found that works quite well, I have done a few runs with it now and no issues, so I will be adding that in for the people that have a hard time finding micropore tape. Just need to recover from last night :lol: Ate a lot of stones :wink:

Happy New Year everyone!


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Re: Pasty Agar Tek [Re: Pastywhyte]
    #19355432 - 01/01/14 04:50 PM (10 years, 1 month ago)

:thumbup:


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Re: Pasty Agar Tek [Re: cronicr]
    #19355743 - 01/01/14 06:15 PM (10 years, 1 month ago)

chronin
how firm r u letting it get? id imagine u can get it all the way firm and put it in because its just going to liquify again in PC, but r u letting it firm up to just to the point where that middle agar blob happens?


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Re: Pasty Agar Tek [Re: blindingleaf] * 1
    #19355762 - 01/01/14 06:19 PM (10 years, 1 month ago)

not really going by how firm it gets just trying to get it to room temp so when you close the lid you don't get condensation build up and everything is starting at the same temp during the pc cycle and cooling at the same temp once you shut the pc off:thumbup:


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Re: Pasty Agar Tek [Re: cronicr]
    #19355826 - 01/01/14 06:38 PM (10 years, 1 month ago)

cool sounds good.
can u explain to me the theory of not letting pc totally cool, i.e. pc drops to 0, but is still hot, so when u take weighted gauge off, steam comes out for maybe 10 seconds.  there was/is talk of how that may be introducing con tams via outside air being sucked in while steam is being released thru top vent.  is this true?

edit: i have also read that if u don't let it cool completely, like in above example or say when PC is at 1psi, and u take weight off, air will also be sucked out of jar thru filter as the steam/air in PC RUSHES to the vent to escape. is this true also, and is this a concern for contam or for something else?


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Edited by blindingleaf (01/01/14 06:40 PM)


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Re: Pasty Agar Tek [Re: blindingleaf]
    #19355860 - 01/01/14 06:49 PM (10 years, 1 month ago)

Quote:

blindingleaf said:
there was/is talk of how that may be introducing con tams via outside air being sucked in while steam is being released thru top vent.  is this true?



Hot air is less dense than cool air, and so as the PC cools it will "suck" air from the surrounding area. 

Autoclaves have compound gauges and DO NOT "suck" air back.  They will eventually enter into a "vacuum" and as the unit cools, negative pressure will rise UNTIL the user opens a relief valve.

If you use proper gas exchange/filter ports, this should introduce little to no issue.  Something as simple as a polyfill ge port should keep everything out.

I think what cronicr is explaining is that IF you start your plastic jars @ room temp, with no condensation then PC and allow to cool, that there should continue to be no condensation once it gets back to room temp.

Glass jars seem to work no matter what process.  I have yet to do anything special with the glass jars.  PC while hot/fluid etc.  I have done it a few ways with glass, and none of them have condensation after a day.  HOWEVER, I have noticed that the metal lids on glass jars produce slightly more condensate than the plastic lids.  All of which vanishes after 24 hours.


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Re: Pasty Agar Tek [Re: HypnotoadCroaked]
    #19355935 - 01/01/14 07:13 PM (10 years, 1 month ago)

ok, i was reading GGMM and he mentions two kinds of PC/autoclave one of which will create a vacuum and the other which will not.  it suggests that if u have the one that does not create the vacuum to take the jars out right when pc drops to 0psi.  i have an AA 914 i believe (the one that fits 7 quarts) with that weight that comes off, is circular and have 5, 10, 15 holes.is the one i have creating  a vacuum?  i'd assume no, at least it doesn't seem like it when i open it, even with weight on top.

GGMM page 132, chapter 15 on generating grain spawn.

I'm asking because i let my jars cool in pc overnight.  whether i use postal tyvek or SFD, i can't help but think the filter is moist in that humid PC, and taking it out say an hour after it has dropped to 0 (so PC still hot and when weight is taken off steam vents for a few seconds) and letting jars cool on shelf would keep the filter dry.  maybe this is all in my head, the concern comes more so when using postal tyvek filters when i run out of SFD lids


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Re: Pasty Agar Tek [Re: blindingleaf]
    #19356192 - 01/01/14 08:43 PM (10 years, 1 month ago)

Quote:

blindingleaf said:
(so PC still hot and when weight is taken off steam vents for a few seconds) and letting jars cool on shelf would keep the filter dry.  maybe this is all in my head, the concern comes more so when using postal tyvek filters when i run out of SFD lids



This is how I do it, for the same reasons I believe.

I hate to keep a jar with a wet filter inside of a PC for hours after it cools.  I like you try to get them out ASAP, so that the filter can dry.

Hot jars fresh out of a pc will continue to vent hot air for a while I noticed.  Even though there is no pressure, the grains are still very hot.  Sometimes I can hear them "crying" as they are equalizing and eventually start pulling air back in as they cool.  I prefer to know that they are pulling through a dry filter vs a wet one.....Wet filters can transfer contaminants.  This is the way I've been doing it, and haven't had issues.


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Re: Pasty Agar Tek [Re: HypnotoadCroaked]
    #19356414 - 01/01/14 10:21 PM (10 years, 1 month ago)

:crazy: whoa, turn your back for 2 hours and find out you missed school:crazy:
i use poly for my filter no foil during the pc have be doing so for god knows how many jars now without issues, the problem with releasing the pressure early is for exactly the reasons you stated and yes if you have a steilizers it always pulls a vacuum while it cools


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Re: Pasty Agar Tek [Re: cronicr]
    #19356480 - 01/01/14 10:42 PM (10 years, 1 month ago)

so whippy and chron...
are u guys waiting till PC cool to the touch to open or just till 0psi?  i remember several grain tek preps that stated to pull jar out a little after 0PSI to shake it and distribute moisture and no mention of possible danger, but then there are several more posts indicating the possible contam issue with this practice.... just made me wonder i guess which i should b doing particularly with the postal tyvek cause i trust my sfd no matter when they're taken out, have had 0 issue with them


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Re: Pasty Agar Tek [Re: blindingleaf]
    #19356504 - 01/01/14 10:48 PM (10 years, 1 month ago)

Quote:

blindingleaf said:

are u guys waiting till PC cool to the touch to open or just till 0psi?



@ 0 psi, my jars come out, and the foil comes off the lid.


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Re: Pasty Agar Tek [Re: HypnotoadCroaked]
    #19356548 - 01/01/14 11:04 PM (10 years, 1 month ago)

Quote:

Whippy said:
Quote:

blindingleaf said:

are u guys waiting till PC cool to the touch to open or just till 0psi?



@ 0 psi, my jars come out, and the foil comes off the lid.



:thumbup: grain jar can come out, agar should stay in


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Re: Pasty Agar Tek [Re: cronicr]
    #19356576 - 01/01/14 11:16 PM (10 years, 1 month ago)

Quote:

cronicr said:

:thumbup: grain jar can come out, agar should stay in



My method for no-pour is the same as my grain.  At 0 psi, out they come.  This may be why my plastic jars always get condensate, while the glass ones are clean and clear.


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Re: Pasty Agar Tek [Re: HypnotoadCroaked]
    #19359682 - 01/02/14 05:09 PM (10 years, 1 month ago)

:smbfacepalm: just did me an oops....
i had a contaminated "plate" after a transfer and was doing up more agar, i decided tochuck the contammed one in the pc with the others...and forgot there was one contammed one in there and proceed to make more transfers....i can see the pc'd mold stuck in the agar lol


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Edited by cronicr (01/02/14 05:14 PM)


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Re: Pasty Agar Tek [Re: cronicr]
    #19359692 - 01/02/14 05:11 PM (10 years, 1 month ago)

it's either genius or :smbfacepalm:


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Re: Pasty Agar Tek [Re: cronicr]
    #19359742 - 01/02/14 05:20 PM (10 years, 1 month ago)

Quote:

cronicr said:
it's either genius or :smbfacepalm:



I don't know where I put my 2 bacterial dishes that I re-pc'd....the only reason they got all iffy like that was because I wanted to test how sterile my microwave was.....

Ill let you know.  I have an idea they have something new growing on them.....


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Re: Pasty Agar Tek [Re: HypnotoadCroaked]
    #19359750 - 01/02/14 05:21 PM (10 years, 1 month ago)

i did that this morning and it just dawned on me a minute ago lol!


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Re: Pasty Agar Tek [Re: HypnotoadCroaked]
    #19359776 - 01/02/14 05:26 PM (10 years, 1 month ago)

Quote:

Whippy said:
Ill let you know.



Well, that was quick.  I found the 2 jars.

One was used for the first xfer from a MS inoculation, and grew out into a very close isolate.  The second dish by coincidence was a transfer from that dish, and is an isolate.

The only reason I know it was them 2 because they have the plastic lids and a slight murkiness to the agar.  Recycling!


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Re: Pasty Agar Tek [Re: HypnotoadCroaked]
    #19359790 - 01/02/14 05:28 PM (10 years, 1 month ago)

:crazy: all that really changed was the color of my agar, it got darker but i cn see the contam in the middle with the chunk from my transfer lol


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Re: Pasty Agar Tek [Re: cronicr]
    #19360255 - 01/02/14 07:03 PM (10 years, 1 month ago)

Quote:

cronicr said:
:smbfacepalm: just did me an oops....
i had a contaminated "plate" after a transfer and was doing up more agar, i decided tochuck the contammed one in the pc with the others...and forgot there was one contammed one in there and proceed to make more transfers....i can see the pc'd mold stuck in the agar lol



:bathtub40lol:


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Re: Pasty Agar Tek [Re: Pastywhyte]
    #19360339 - 01/02/14 07:24 PM (10 years, 1 month ago)

:lmafo:
took me all fucking day to realize it, then i went and looked at them and was like WTF IS THAT IN THERE!:smbfacepalm:


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Re: Pasty Agar Tek [Re: cronicr]
    #19370248 - 01/04/14 08:52 PM (10 years, 1 month ago)

You might want to check these out Pasty.

I came across them at the dollar store yesterday, six for a buck and autoclavable (polypropelene).  This breaks down to roughly 17 cents a piece (or 83 bucks for 500). They measure about 40mm x 45mm wide and hold 35ml.

I have run a test batch and they did hold up at 15psi for 15 min.  I am almost tempted to switch from disposable petri's at this price, although more trials are needed to see how breathable the lids are (not sure if I should be drilling mini holes with poly, as they are so tiny!). 

Here are some pics below.

Packaging



Size comparison



Measurements





Polypropelene stamp



120 of them neatly packed away in a 30x20x10cm little plastic bin.



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Re: Pasty Agar Tek [Re: Delection]
    #19370310 - 01/04/14 09:11 PM (10 years, 1 month ago)

:rockon:
my dollar stores don't have any pp5's good for the use but those kick ass:thumbup:


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Re: Pasty Agar Tek [Re: cronicr]
    #19370337 - 01/04/14 09:17 PM (10 years, 1 month ago)

Quote:

cronicr said:
:rockon:
my dollar stores don't have any pp5's good for the use but those kick ass:thumbup:




Your dollar is my dollar store cronicr, there are 4 locations on the island (Campbell River, Port Alberni, Nanaimo and Victoria).

http://www.dollarama.com/store-locator/


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Re: Pasty Agar Tek [Re: Delection]
    #19370343 - 01/04/14 09:19 PM (10 years, 1 month ago)

yeah i was just there the other day i didn't see them:shrug: maybe i'll have to check again


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Re: Pasty Agar Tek [Re: Delection]
    #19370346 - 01/04/14 09:20 PM (10 years, 1 month ago)

Delection, those are nice looking, I'd use 'em!
Just found me some more glass though (for some reason I love Ball jars).  They're the 4oz "dry herb jars".  Pretty awesome and way easier to see through than the ones I was using before.


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Re: Pasty Agar Tek [Re: Delection]
    #19370352 - 01/04/14 09:21 PM (10 years, 1 month ago)

Those are interesting,  a little small in diameter for my liking but could probably be good for use as a pseudo slant for long term storage of cultures :thumbup:


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Re: Pasty Agar Tek [Re: cronicr]
    #19370358 - 01/04/14 09:23 PM (10 years, 1 month ago)

Quote:

cronicr said:
yeah i was just there the other day i didn't see them:shrug: maybe i'll have to check again




Well you wont find any in Nanaimo, I bought them all :grin:


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Re: Pasty Agar Tek [Re: Pastywhyte]
    #19370377 - 01/04/14 09:27 PM (10 years, 1 month ago)

Quote:

Pastywhyte said:
Those are interesting,  a little small in diameter for my liking but could probably be good for use as a pseudo slant for long term storage of cultures :thumbup:




45 mm diameter to small?  Ive been digging culture out of 17 mm tubes without issue, even if I was isolating 45mm seems big enough.  I will keep you updated on how they work.


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Re: Pasty Agar Tek [Re: Delection]
    #19370410 - 01/04/14 09:37 PM (10 years, 1 month ago)

Quote:

Delection said:
Quote:

Pastywhyte said:
Those are interesting,  a little small in diameter for my liking but could probably be good for use as a pseudo slant for long term storage of cultures :thumbup:




45 mm diameter to small?  Ive been digging culture out of 17 mm tubes without issue, even if I was isolating 45mm seems big enough.  I will keep you updated on how they work.




I agree, they're big enough for my use.  Dunno why I'd need more?  I rarely use more than 10 or so wedges from a dish, and that's when I'm sharing!


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Re: Pasty Agar Tek [Re: Delection]
    #19370544 - 01/04/14 10:15 PM (10 years, 1 month ago)

Fair enough but I like a little more room than that. Just a personal preference. No reason why they wouldn't work. I think they would have a lot of potential as a slant. I will keep an eye out for them regardless and give em a try if I find them both as a slant and as a plate :thumbup:


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Re: Pasty Agar Tek [Re: Pastywhyte]
    #19370558 - 01/04/14 10:19 PM (10 years, 1 month ago)

Yeah I could see 'em as a slant!

Either way you gotta ditch the plastic herb shaker lids they come with, and use those for... herbs.  Or herb.  Or somethin'.

I just fished out some old one hole lids and stuffed 'me with polyfill, hopefully that works this time around.


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Re: Pasty Agar Tek [Re: Pastywhyte]
    #19370561 - 01/04/14 10:19 PM (10 years, 1 month ago)

nice they are kinda small to me too but shit keep us posted :thumbup:


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Re: Pasty Agar Tek [Re: tripdawg420]
    #19370571 - 01/04/14 10:23 PM (10 years, 1 month ago)

They really ain't any smaller than the glad mini rounds you're using in the OP - unless we're thinking about different items?  I dunno, I'll take a side by side pic in the morning but they look about the same size to me :shrug:


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Re: Pasty Agar Tek [Re: Forrester]
    #19370682 - 01/04/14 10:58 PM (10 years, 1 month ago)

Quote:

Forrester said:

Either way you gotta ditch the plastic herb shaker lids they come with, and use those for... herbs.  Or herb.  Or somethin'.

I just fished out some old one hole lids and stuffed 'me with polyfill, hopefully that works this time around.




Herb shaker lids? they are just clear snap on lids, with no holes.


Quote:

Forrester said:
They really ain't any smaller than the glad mini rounds you're using in the OP - unless we're thinking about different items?  I dunno, I'll take a side by side pic in the morning but they look about the same size to me :shrug:




These are only 45mm wide, the glad mini rounds have to be at least 100-150mm wide.


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Re: Pasty Agar Tek [Re: Delection]
    #19370853 - 01/04/14 11:56 PM (10 years, 1 month ago)

Actually the glad mini rounds are 55mm at the bottom and widen slightly as they go up,  which IMO makes it easier to get my tools in with little risk of touching  the edges. They are the perfect size for my needs.

Really no pour agar can be done in a variety of containers, whatever works for a person is :cool: I like my minis as they are the perfect shape, no messing with screw tops, etc.


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Re: Pasty Agar Tek [Re: Delection]
    #19371524 - 01/05/14 06:31 AM (10 years, 1 month ago)

Quote:

Pastywhyte said:
Really no pour agar can be done in a variety of containers, whatever works for a person is :cool: I like my minis as they are the perfect shape, no messing with screw tops, etc.




I like 'em too, didn't even wrap in foil and they've been sitting in my room in open air.  So far no contams have managed to sneak in thru the snap on lids!

Quote:

Delection said:
These are only 45mm wide, the glad mini rounds have to be at least 100-150mm wide.




You must have gotten the bigger ones, mine I think are the 55mm ones like Pasty said.


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Re: Pasty Agar Tek [Re: Forrester]
    #19371541 - 01/05/14 06:43 AM (10 years, 1 month ago)

forrester, do u mod the lid still like in the OP when u pc if u don't use foil?  i remember pasty and I talking about (thru a PM, sry pasty don't hate me :smile: ) lessening the foil, and IIRC we both weren't sure if it would work, for both wetness of micropore and longevity of sterility.  i don't need to worry about foil now (got a HUGE roll for x mas from my sister oddly enough hahah) but i'd like to know regardless.


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Re: Pasty Agar Tek [Re: blindingleaf]
    #19371660 - 01/05/14 07:38 AM (10 years, 1 month ago)

I didn't have micropore tape, so I siliconed in some little circles of tyvek instead.  2 out of 8 of 'em ended up getting crushed so I guess it's not quite enough airflow but it worked ok.  I like the tyvek cause it's pretty water resistant, might just try bigger holes next time.


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Re: Pasty Agar Tek [Re: Forrester]
    #19371683 - 01/05/14 07:45 AM (10 years, 1 month ago)

Quote:

Forrester said:
I didn't have micropore tape, so I siliconed in some little circles of tyvek instead.  2 out of 8 of 'em ended up getting crushed so I guess it's not quite enough airflow but it worked ok.  I like the tyvek cause it's pretty water resistant, might just try bigger holes next time.




How big of a hole did you use? I just set up a bunch of easy plates with 1/4" holes and tight-as-possible polyfill wads.


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Re: Pasty Agar Tek [Re: elasticaltiger]
    #19371785 - 01/05/14 08:34 AM (10 years, 1 month ago)

Quote:

elasticaltiger said:
How big of a hole did you use? I just set up a bunch of easy plates with 1/4" holes and tight-as-possible polyfill wads.




I did somewhere around 1/4" on the mini rounds with tyvek.
The ball jar ones I did last night I tried exactly what you said, stuffed polyfill as tight as I could, hopefully that doesn't let anything in...


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Re: Pasty Agar Tek [Re: Forrester]
    #19372075 - 01/05/14 10:00 AM (10 years, 1 month ago)

i use poly and never use foil in the pc:crazy:


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Re: Pasty Agar Tek [Re: Delection]
    #19377278 - 01/06/14 01:37 PM (10 years, 1 month ago)

Quote:

Delection said:
You might want to check these out Pasty.

I came across them at the dollar store yesterday, six for a buck and autoclavable (polypropelene).  This breaks down to roughly 17 cents a piece (or 83 bucks for 500). They measure about 40mm x 45mm wide and hold 35ml.

I have run a test batch and they did hold up at 15psi for 15 min.  I am almost tempted to switch from disposable petri's at this price, although more trials are needed to see how breathable the lids are (not sure if I should be drilling mini holes with poly, as they are so tiny!). 

Here are some pics below.

Packaging



Size comparison



Measurements





Polypropelene stamp



120 of them neatly packed away in a 30x20x10cm little plastic bin.






Lmfao I saw these too!! Except the ones I saw were rectangular. How did they hold up? Any lid mod? How are they to work with, like getting a piece for xfer?

Finally starting agar today:woot:


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Re: Pasty Agar Tek [Re: magickspore]
    #19377408 - 01/06/14 02:10 PM (10 years, 1 month ago)

my contaminated recycled agar is looking great:homerdoh3:


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Re: Pasty Agar Tek [Re: magickspore]
    #19377653 - 01/06/14 03:08 PM (10 years, 1 month ago)

Quote:

magickspore said:

Lmfao I saw these too!! Except the ones I saw were rectangular. How did they hold up? Any lid mod? How are they to work with, like getting a piece for xfer?

Finally starting agar today:woot:




Still performing some tests on them as we speak, they held up well to 15psi and no warping. We will have to see.

Has anyone tried agar work with those little restaurant containers similar to the ones below?





They are Polystyrene, but I can pick up 2500 of them (with lids) for $100.


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Re: Pasty Agar Tek [Re: Delection]
    #19377776 - 01/06/14 03:30 PM (10 years, 1 month ago)

we have those to where i work, but they are PP2...are those PP5?
if so, i'd still elevate the whole rack they sit on with some 1/2 pints so its just steam touching them.


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Re: Pasty Agar Tek [Re: blindingleaf]
    #19378105 - 01/06/14 04:58 PM (10 years, 1 month ago)

Quote:

blindingleaf said:
we have those to where i work, but they are PP2...are those PP5?
if so, i'd still elevate the whole rack they sit on with some 1/2 pints so its just steam touching them.




:lolwut:

The water and steam are the same temp.


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Re: Pasty Agar Tek [Re: FrankHorrigan]
    #19378120 - 01/06/14 05:01 PM (10 years, 1 month ago)

haha yea i guess ur right.  but i thought with the pasty tek we have to raise them a bit above the water line?  i always do anyways...i guess to prevent over boiling?

edit:  just re read tek, I'm an idiot :shrug:  no mention of raising above water level.  guess I'm paranoid too haha


Edited by blindingleaf (01/06/14 05:06 PM)


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Re: Pasty Agar Tek [Re: blindingleaf]
    #19378137 - 01/06/14 05:05 PM (10 years, 1 month ago)

Thank you pasty! This Tech single-handedly got me into agar. I am having a little problem with condensation building up on the sides of the containers, But not enough to ruin the media I think


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Re: Pasty Agar Tek [Re: Alwaysanoob1]
    #19378207 - 01/06/14 05:24 PM (10 years, 1 month ago)

Is it supposed be the same consistency as mashed potatoes? I thought I followed the recipe right but looking back I'm not sure. I probably made it to thick, but I thought I'd just ask real quick.


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Re: Pasty Agar Tek [Re: magickspore]
    #19378890 - 01/06/14 07:24 PM (10 years, 1 month ago)

Quote:

blindingleaf said:
haha yea i guess ur right.  but i thought with the pasty tek we have to raise them a bit above the water line?  i always do anyways...i guess to prevent over boiling?

edit:  just re read tek, I'm an idiot :shrug:  no mention of raising above water level.  guess I'm paranoid too haha




You should raise the container above the water, guess I took it for granted that people would do that.  I will edit the OP.


Quote:

Alwaysanoob1 said:
Thank you pasty! This Tech single-handedly got me into agar. I am having a little problem with condensation building up on the sides of the containers, But not enough to ruin the media I think




Glad your on the agar wagon :rockon: A little condensation won't hurt nothing and will usually clear up in a day or two :super:


Quote:

magickspore said:
Is it supposed be the same consistency as mashed potatoes? I thought I followed the recipe right but looking back I'm not sure. I probably made it to thick, but I thought I'd just ask real quick.




It should be like jello :lol:


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Re: Pasty Agar Tek [Re: Pastywhyte]
    #19379023 - 01/06/14 07:49 PM (10 years, 1 month ago)

Quote:

Pastywhyte said:
Quote:

magickspore said:
Is it supposed be the same consistency as mashed potatoes? I thought I followed the recipe right but looking back I'm not sure. I probably made it to thick, but I thought I'd just ask real quick.




It should be like jello :lol:




lol ok that's what I thought I must have done something wrong. Because it was like dry mashed taters :rofl:.

Other than that everything seems ok. I let them reach room temp, loaded PC, PCd for about 45-50 min (I did that because my regulator was only holding 11-13 psi. I'll have to look into that.) Now its cooling got about an 1/8th to 1/4" on the bottom of my containers. Once they're done cooling, time to have some fun.


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Re: Pasty Agar Tek [Re: Delection]
    #19379283 - 01/06/14 08:31 PM (10 years, 1 month ago)

Quote:

Delection said:
Quote:

magickspore said:

Lmfao I saw these too!! Except the ones I saw were rectangular. How did they hold up? Any lid mod? How are they to work with, like getting a piece for xfer?

Finally starting agar today:woot:




Still performing some tests on them as we speak, they held up well to 15psi and no warping. We will have to see.

Has anyone tried agar work with those little restaurant containers similar to the ones below?





They are Polystyrene, but I can pick up 2500 of them (with lids) for $100.




cool idea, tho i strongly doubt jello shot cups will hold up in the pressure cooker.


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Re: Pasty Agar Tek [Re: J. Jack Flash] * 1
    #19379381 - 01/06/14 08:45 PM (10 years, 1 month ago)

Those will melt to shit. I wouldn't waste my time.


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Re: Pasty Agar Tek [Re: FrankHorrigan]
    #19379620 - 01/06/14 09:43 PM (10 years, 1 month ago)

People use them, but straight from the sleeve they come in, and only if it is unpunctured sleeve. Just like disposable petri dishes


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Re: Pasty Agar Tek [Re: mushrume man]
    #19379661 - 01/06/14 09:51 PM (10 years, 1 month ago)

Well then it depends on if the cups are sterile and how well the user can pour agar into them quickly and cleanly. 

I assumed the member was asking if they could use them for no-pour in the PC.


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Re: Pasty Agar Tek [Re: FrankHorrigan]
    #19380604 - 01/07/14 01:44 AM (10 years, 1 month ago)

Quote:

FrankHorrigan said:
Well then it depends on if the cups are sterile and how well the user can pour agar into them quickly and cleanly. 

I assumed the member was asking if they could use them for no-pour in the PC.




No I should have clarified.  I realize these wouldn't survive a PC, I was wondering if anyone has ever attempted using these after media was already PC'd - I am not sure how sterile they would come, but maybe using some antibiotic agar you might get away with it.


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Re: Pasty Agar Tek [Re: Delection] * 1
    #19384215 - 01/07/14 06:25 PM (10 years, 1 month ago)

Antiobiotic agar won't protect you from mold spores, sadly.


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Re: Pasty Agar Tek [Re: Psilicon]
    #19388739 - 01/08/14 03:31 PM (10 years, 1 month ago)

Quote:

van der griegen said:
Antiobiotic agar won't protect you from mold spores, sadly.




I know gentamicin does not kill mold spores, thats stating the obvious..  I guess I am going to have to try them and find out myself.


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Re: Pasty Agar Tek [Re: Delection]
    #19388791 - 01/08/14 03:40 PM (10 years, 1 month ago)

When I get home I will take a pic of tetracycline agar covered in mold for ya :wink:


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Re: Pasty Agar Tek [Re: Pastywhyte]
    #19393759 - 01/09/14 01:41 PM (10 years, 1 month ago)

Okay, so the agar should be thick and jelly like, correct? I've made one missed attempt by using a watery agar, so im guessing this is a waste and I should start again.. I guess I was a little to baked when making the agar.

What tek would you go too from here, for adding spores to the mix? I had a half of a syringe and several spore prints from my previous grow. I've read several teks on how to go about this process, but being this is your tek I would like to know what your preffered method is.


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Re: Pasty Agar Tek [Re: Galaxy]
    #19394001 - 01/09/14 02:42 PM (10 years, 1 month ago)

To inoculate with ms solution I like to first heat my loop red hot and then use it to melt a little divot into the surface of the agar. Then use a single drop right into the divot. This will help keep it from sliding around the plate. Leave it in the SAB for a day or two which will absorb the water into the agar, leaving the spores in one spot. This helps keep any bacteria confined and will make it easier to transfer away from.

With a print, flame your loop or scalpel, then cool it in the agar. Scrape up a tiny amount of spores,  then drag the loop or tip of scalpel across the surface. You could also hold the print above the plate and flick the spores onto the plate. This can be a little  risky for introducing contams but does allow you to identify faster genetics. Ultimately you will need to experiment a bit and decide what works for you :super:


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Re: Pasty Agar Tek [Re: Delection]
    #19395088 - 01/09/14 06:14 PM (10 years, 1 month ago)

Quote:

Delection said:
Quote:

FrankHorrigan said:
Well then it depends on if the cups are sterile and how well the user can pour agar into them quickly and cleanly. 

I assumed the member was asking if they could use them for no-pour in the PC.




No I should have clarified.  I realize these wouldn't survive a PC, I was wondering if anyone has ever attempted using these after media was already PC'd - I am not sure how sterile they would come, but maybe using some antibiotic agar you might get away with it.




seen it at mycotopia. but just poured.


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Re: Pasty Agar Tek [Re: Yuri.Pono]
    #19422297 - 01/15/14 10:57 AM (10 years, 1 month ago)

Thanks for this tek. I'm kind of sick if working with short half pint jars. My only master jar broke last night and I'm devestated that I lost my culture. I had a couple of agar jars with culture from fruit that I threw on WBS so hopefully all is not lost but instead of having 24 jars inoculated I have 5 or 6. I'm slightly devestated but I've got plenty of prints so I just gotta get cracking on agar. However I'm sure to be weeks to a month or more behind now. Love the food coloring idea as well. I'm bout to head to the dollar store.  Hopefully they have them there. :thumbup:


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Re: Pasty Agar Tek [Re: DeadPhan]
    #19422486 - 01/15/14 11:59 AM (10 years, 1 month ago)

:manofapproval:


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Re: Pasty Agar Tek [Re: Pastywhyte]
    #19422546 - 01/15/14 12:12 PM (10 years, 1 month ago)

After using this tek along with a little advice from Cronic about letting my PP5s cool before PCing Im finally starting to have some success with agar. Thanx guys. I was starting to lose my mind a little.


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Re: Pasty Agar Tek [Re: MudaFuka]
    #19422697 - 01/15/14 12:52 PM (10 years, 1 month ago)

if u end up finding ur mind, put 5g of it in pasty agar recipe and u will see crazy rhino growth!


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Re: Pasty Agar Tek [Re: blindingleaf]
    #19423050 - 01/15/14 02:10 PM (10 years, 1 month ago)

Quote:

blindingleaf said:
if u end up finding ur mind, put 5g of it in pasty agar recipe and u will see crazy rhino growth!



Are you saying put five grams of cubes in the agar recipe?


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Re: Pasty Agar Tek [Re: DeadPhan]
    #19423061 - 01/15/14 02:11 PM (10 years, 1 month ago)

haha no, mudafucker said he was losing his mind, so i said if u find it, put 5 g of ur mind in the recipe.  it was a joke...guess not a funny one  :shrug:


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Re: Pasty Agar Tek [Re: blindingleaf] * 1
    #19423218 - 01/15/14 02:45 PM (10 years, 1 month ago)

Quote:

blindingleaf said:
haha no, mudafucker said he was losing his mind, so i said if u find it, put 5 g of ur mind in the recipe.  it was a joke...guess not a funny one  :shrug:



I thought it was pretty funny.


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Re: Pasty Agar Tek [Re: MudaFuka]
    #19423549 - 01/15/14 03:51 PM (10 years, 1 month ago)

cool, thought it went over ppls heads haha.


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Re: Pasty Agar Tek [Re: blindingleaf]
    #19424147 - 01/15/14 06:15 PM (10 years, 1 month ago)

Does this look like a good deal on the mini rounds?  They didn't have at dollar store. Decided I check amazon. I'm an amazon prime member. So free shipping.

Link here


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Edited by DeadPhan (01/15/14 06:20 PM)


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Re: Pasty Agar Tek [Re: DeadPhan]
    #19424197 - 01/15/14 06:27 PM (10 years, 1 month ago)

Yeah I would say that's good deal :thumbup:


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Re: Pasty Agar Tek [Re: Pastywhyte]
    #19424396 - 01/15/14 07:19 PM (10 years, 1 month ago)

I would say that's a lot of agar dishes.  I've been thinking of getting another dozen or so but daaamn.


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Re: Pasty Agar Tek [Re: Generic]
    #19427948 - 01/16/14 12:58 PM (10 years, 1 month ago)









this is not what it looks like (or maybe it is...)

so this holiday season was crazy for me, let 28 plates go too long.  had to toss them and start a new.  this is really a testament to not only my laziness (I'm on top of stuff now), and the lack of space in my fridge, but also to Pasty's TEK.  these ALL lasted OVER A MONTH AND A HALF with NO refrigeration, survived multiple transfers, dust from smoke, cats, and a dog, and no extra seal, nada.  i didn't even keep the unused ones in fridge till use, they all stayed room temp with no extra filter, in a cardboard box.  just wanna say thx again pasty, ur tek is awesome, and i wanted to post these here so ppl kno u can almost abuse these things if u make them to a t like the tek, and have little contam (if any, maybe i was lucky...?).  In the trash bag pic, thats not a brown contam, thats shiitaki left too long, and browning up a bit in case anyone wondered.


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Re: Pasty Agar Tek [Re: Pastywhyte]
    #19427958 - 01/16/14 01:01 PM (10 years, 1 month ago)

This tek's purpose is to be entry-level, yes?
It does a good job of that in form and instruction, but I feel it can be trimmed even further.

Yours:
Quote:

You will need:
-1 pack of Glad mini rounds
-micro pore tape
-a drill with a 1/4" bit or very hot nail
-paper towels
-Foil
-PC
-Bar of agar agar
-Potato flakes
-Karo or Honey
-Coffee grinder
-And of course a PC, even a shitty ass one will work



Mine:
Quote:

You will need:
-Ziploc twist-top pint containers
-Agar-agar powder
-Grainwater from brown rice/grass seed prep, or malt/karo/potato flakes etc.
-And of course a PC




Maybe I missed it, but why do you use foil for instance?


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Re: Pasty Agar Tek [Re: Violet] * 1
    #19427968 - 01/16/14 01:04 PM (10 years, 1 month ago)

pasty can probably answer better, but i think its so the micropore does not get too wet and water drip into containers.  i had asked him this (in PM) kinda about shaving off the amount of foil.  i tried with no foil, too much water, but this is what i came up with



instead of wrapping the entire container.  works for me, just gotta really press the foil under the lip hard.  i just wanted to save foil  :shrug:


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Re: Pasty Agar Tek [Re: Violet]
    #19427977 - 01/16/14 01:09 PM (10 years, 1 month ago)

Quote:

Violet said:
This tek's purpose is to be entry-level, yes?
It does a good job of that in form and instruction, but I feel it can be trimmed even further.

Yours:
Quote:

You will need:
-1 pack of Glad mini rounds
-micro pore tape
-a drill with a 1/4" bit or very hot nail
-paper towels
-Foil
-PC
-Bar of agar agar
-Potato flakes
-Karo or Honey
-Coffee grinder
-And of course a PC, even a shitty ass one will work



Mine:
Quote:

You will need:
-Ziploc twist-top pint containers
-Agar-agar powder
-Grainwater from brown rice/grass seed prep, or malt/karo/potato flakes etc.
-And of course a PC




Maybe I missed it, but why do you use foil for instance?



the twist tops are handy because you don't need to alter the lids but the mini rounds take up a lot les space. both methods are sound. to each there own


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Re: Pasty Agar Tek [Re: MudaFuka]
    #19428061 - 01/16/14 01:26 PM (10 years, 1 month ago)

Quote:

MudaFuka said:
the twist tops are handy because you don't need to alter the lids but the mini rounds take up a lot les space.



Even "taking up a lot less space" is partially a drawback, because they have so much less grow-out area.
I get to an isolate in very few dish transfers because mine have far more area to run out and begin clearer sectoring.
And once a dish is grown out, I can use it to inoculate 20 things instead of 7 or 8.
Additionally, I see that everyone's agar is Very deep in these mini-rounds due to the curvature towards the bottom.  They'd have even less area to grow out if agar were used conservatively.  However mine have as little as a normal petri dish with the wide area.

I can only see taking up less space as being assuredly a good thing in the case of refrigerator storage as Pasty mentioned.
Yet I have had more screw-top dished cultures in the fridge than I even want to, and it hasn't become an issue for me except when I have 20 cultures awaiting the results of elimination testing, in which case fridge storage is still optional...


Quote:

blindingleaf said:
pasty can probably answer better, but i think its so the micropore does not get too wet and water drip into containers.



I'm sure this is the (or at least a) correct answer.  It's just a bit of a shame though, needing foil because of needing micropore tape because of needing to modify because of not choosing ready-to-use containers...


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Re: Pasty Agar Tek [Re: Violet]
    #19428136 - 01/16/14 01:38 PM (10 years, 1 month ago)

Quote:

Violet said:
Quote:

MudaFuka said:
the twist tops are handy because you don't need to alter the lids but the mini rounds take up a lot les space.



Even "taking up a lot less space" is partially a drawback, because they have so much less grow-out area.
I get to an isolate in very few dish transfers because mine have far more area to run out and begin clearer sectoring.
And once a dish is grown out, I can use it to inoculate 20 things instead of 7 or 8.
Additionally, I see that everyone's agar is Very deep in these mini-rounds due to the curvature towards the bottom.  They'd have even less area to grow out if agar were used conservatively.  However mine have as little as a normal petri dish with the wide area.

I can only see taking up less space as being assuredly a good thing in the case of refrigerator storage as Pasty mentioned.
Yet I have had more screw-top dished cultures in the fridge than I even want to, and it hasn't become an issue for me except when I have 20 cultures awaiting the results of elimination testing, in which case fridge storage is still optional...







not every one has a large PC. even with the lid off and the dishes stacked inside one another I can fit fewer screw tops in my PC than mini rounds. Iv found I use much more agar filling screw tops than mini rounds. the extra surface aria is nice and I love the lids. The truth is I prefer the screw tops but I don't understand why you seem to care so much about what other people use.


Edited by MudaFuka (01/16/14 01:40 PM)


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Re: Pasty Agar Tek [Re: MudaFuka]
    #19428156 - 01/16/14 01:43 PM (10 years, 1 month ago)

Quote:

MudaFuka said:
I don't understand why you seem to care so much about what other people use.



Cut that out.  I'm just talking about materials and process.  I don't "care so much about what other people use" anymore than anyone else does discussing what they do and why.

Quote:

MudaFuka said:
not every one has a large PC.



I have an AA910. Very small.  I doubt that more than a handful of people buy AAs smaller than 916.

Quote:

MudaFuka said:
even with the lid off and the dishes stacked inside one another I can fit fewer screw tops in my PC than mini rounds.



I don't doubt this, I simply don't think it totally makes up for the other factors.

Such as, "once a dish is grown out, I can use it to inoculate 20 things instead of 7 or 8."
Do you fit Over Double the glad-rounds in the pc?

Personally I don't even use all the dishes I make in my cooker.  Some of mine sit in sterile airflow for weeks before I find something to drop in them.

Quote:

MudaFuka said:
Iv found I use much more agar filling screw tops than mini rounds.



You use less than ~22mL in the mini rounds?

Even if so, the lesser amount surely doesn't make up for the proportion of lesser area.


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Re: Pasty Agar Tek [Re: Violet]
    #19428198 - 01/16/14 01:49 PM (10 years, 1 month ago)

sorry I wasn't trying to be rude. You just sounded a bit condescending in your previous replies.


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Re: Pasty Agar Tek [Re: MudaFuka]
    #19428594 - 01/16/14 03:11 PM (10 years, 1 month ago)

Well I have used the screwtops and didn't like them. They are not nearly as easy to manipulate in the SAB, I can hold and open my minis with one hand.Also I don't like the depth of the ziplocks too easy to touch the rim with my scalpel unless I reach over the mouth. Plus if you are going to G2G how many masters do you really need? 1 quart can easily be expanded to1000 by the third expansion :shrug: personaly I'm not a fan of cutting more than a few wedges from single plate anyway. Might feel differently if I had a hood, but I don't.

This is not a contest. If someone prefers ziplocks or jars or pour agar cool. As long as there doing agar I'm happy. But if someone tries agar and has a hard time with one method or another container,  this is another option for them to try before they give up on it.


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Re: Pasty Agar Tek [Re: Pastywhyte] * 1
    #19428722 - 01/16/14 03:33 PM (10 years, 1 month ago)

Personally Pasty, if I hadnt of found this tek I would still be waiting for a large flowhood and petri's etc instead of having everything I need minus a flush from my current tub so I can take a clone :smile:.

Its value in that alone, getting peeps to jump into agar earlier is priceless. The simplicity of the tek and readily available materials really opened my mind to the possibilities when previously I wasnt even entertaining the thought. Thanks for doing it.


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Re: Pasty Agar Tek [Re: Valyr] * 1
    #19428739 - 01/16/14 03:36 PM (10 years, 1 month ago)

You just need a SAB to work with petris and agar :wink:


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Re: Pasty Agar Tek [Re: FrankHorrigan]
    #19428765 - 01/16/14 03:40 PM (10 years, 1 month ago)

Yeah but it was pasty's tek that made it dawn on me. I know it should have all on its own but for some reason I was just stuck in that mental wall of cant do it without.... blah blah blah


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Re: Pasty Agar Tek [Re: Valyr] * 1
    #19428947 - 01/16/14 04:15 PM (10 years, 1 month ago)

Sometimes we just can't see the forest :wink: If I can get just one person to start using agar with this writeup, it has done its job.


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Re: Pasty Agar Tek [Re: Pastywhyte]
    #19429829 - 01/16/14 07:15 PM (10 years, 1 month ago)

I have been using the twisties, which are huge by comparison to the minis, but i feel they stack better.  I have had some instances nesting them in the cooker where they seal between each other when they cool and deform from the vacuum created.  I figure using foil or something to make a gap will solve this.  And i agree, they're a bitch in the still air box, but so far, so is most everything else.

I bought some minis, and think the lids can be cracked for sterilizing, so the lid mods wouldn't be necessary.  Haven't tested this idea, tho.  Based on my experience with the twisties, i'm convinced gas exchange isn't necessary.

My current thinking is the twisties are better for growing out for isolating sectors, while the minis are better for growing noc wedges.  An entire mini plate can be dropped in a jar and shaken up.  RR dropped entire thirds from y plates into grain jars, gave me the idea.


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Re: Pasty Agar Tek [Re: J. Jack Flash]
    #19430095 - 01/16/14 08:18 PM (10 years, 1 month ago)

yea u can def drop a whole snap lids worth of agar into a grain jar (cut it up first though).  i tried the pasty tek with no tin foil, water got thru the micropore.  when i tried it with foil covering the cracked lid and no hole, water still got in.  that hole is not for GE in this TEK, its meant for gas to escape when the container is actually cooking (correct me if I'm wrong though pasty).  if u have luck with no lid mod, let me know.  if u wrap the entire container with foil (like the OP tek says) u can probably get away with a cracked, no hole lid.  but thats a lot of foil.
post results if it works!


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Re: Pasty Agar Tek [Re: blindingleaf]
    #19430238 - 01/16/14 08:45 PM (10 years, 1 month ago)

Quote:

blindingleaf said:
yea u can def drop a whole snap lids worth of agar into a grain jar (cut it up first though).  i tried the pasty tek with no tin foil, water got thru the micropore.  when i tried it with foil covering the cracked lid and no hole, water still got in.  that hole is not for GE in this TEK, its meant for gas to escape when the container is actually cooking (correct me if I'm wrong though pasty).  if u have luck with no lid mod, let me know.  if u wrap the entire container with foil (like the OP tek says) u can probably get away with a cracked, no hole lid.  but thats a lot of foil.
post results if it works!




Your right, the hole is not for GE its to prevent a vacuum from crushing the container. I always buy a massive roll from costco for cheap, so using a square per container never really bothered me. Since I use SFD for my jar lids now I don't really use much foil so a roll lasts for a more than a hundred plates or so. You could use SFD for these, but really a few squares of micropore and some foil does not really bother me much. Plus I like the fact that once you unwrap them, the container itself is relatively sterile, one less thing to sanitize before you put it into the box.

Quote:

FrankHorrigan said:
You just need a SAB to work with petris and agar :wink:




Agreed, I just hate pouring in a SAB. Would rather do the no pour myself. Probably gets easier with practice, but the only time I find I need pour agar is for antibiotics (which I recently started with and they are worth the hassel of pour agar), and I actually prefer how much easier the mini rounds are for me to handle compared to a petri in the SAB. Maybe I just built up some good technique with the minis, but I feel so clumsy with real petris :shrug:


Edited by Pastywhyte (01/16/14 09:14 PM)


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Re: Pasty Agar Tek [Re: Pastywhyte]
    #19430297 - 01/16/14 08:57 PM (10 years, 1 month ago)

cool man.  i'll be sure and post up, especially if it fails.  it'll be a funny sight... a dozen or so smushed cups of seaweed goo. 

also, i tried operating the minicups one handed.  i guess my hands are too small to make it work, i can't open them one handed.  how do you do it?  cause i'm sure it's real easy to explain in text format.


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Re: Pasty Agar Tek [Re: Pastywhyte]
    #19430360 - 01/16/14 09:04 PM (10 years, 1 month ago)

Can I do no pour like this in 4 oz (1/4 pint) glass wide mouths? If so I assume you'd just wanna make an injection port and leave the lid loose with the seal down so you can seal it afterwards or Does agar need GE at that stage?


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Re: Pasty Agar Tek [Re: J. Jack Flash]
    #19430393 - 01/16/14 09:11 PM (10 years, 1 month ago)

Quote:

J. Jack Flash said:
also, i tried operating the minicups one handed.  i guess my hands are too small to make it work, i can't open them one handed.  how do you do it?  cause i'm sure it's real easy to explain in text format.




I just hold the lid (already cracked so its loose) with my thumb and forefinger, then cup the rest of it with my other fingers and palm.

I guess I should mention that I'm 6'3" so maybe my hands are just that much bigger.


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Re: Pasty Agar Tek [Re: Happy Littletree]
    #19430467 - 01/16/14 09:22 PM (10 years, 1 month ago)

Quote:

Happy Littletree said:
Can I do no pour like this in 4 oz (1/4 pint) glass wide mouths? If so I assume you'd just wanna make an injection port and leave the lid loose with the seal down so you can seal it afterwards or Does agar need GE at that stage?




You sure can, I have done so myself and they work quite well. Just they are usually pricey, I dislike fumbling with rings and lids, and the sides are hard to see through. You don't need an injection port. Just a hole for the vacuum and a filter of some kind. You need to lift the lid to make transfers anyway. They were what I used (along with half pints, fancy jelly jars, screwtop ziplocks, and poptop ziplocks) before I found the glad minis, which at first I never tried cause I thought they were too small :lol:


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Re: Pasty Agar Tek [Re: Pastywhyte]
    #19430567 - 01/16/14 09:36 PM (10 years, 1 month ago)

So I do have to have a hole and filter not just the jar lid left loose? The reason I ask again is it seems like petris wouldnt have GE and they do fine and the lid cracked should more than take care of the pressure issue. These have a nice clear side to see through unlike most, luckily. I had intended on using these for brf cakes as I couldn't find the ones I need but after I found out about the pp5 I got some small rubbermaids with screw lids for that. Thanks for your help and advice!


Edited by Happy Littletree (01/16/14 09:39 PM)


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Re: Pasty Agar Tek [Re: Happy Littletree]
    #19430732 - 01/16/14 10:06 PM (10 years, 1 month ago)

Hate to mutilate my lids if I don't have to :laugh:


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Re: Pasty Agar Tek [Re: Happy Littletree]
    #19430753 - 01/16/14 10:09 PM (10 years, 1 month ago)

petri's do have ge


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Re: Pasty Agar Tek [Re: cronicr]
    #19430918 - 01/16/14 10:40 PM (10 years, 1 month ago)

Even if the lid is cracked it will still probably suck shut when the vacuum builds. Its not mutilation, its modification. We do lots of that in this hobby.


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Re: Pasty Agar Tek [Re: Pastywhyte]
    #19431099 - 01/16/14 11:18 PM (10 years, 1 month ago)

gotcha.. just wanted to be certain. I been reading a ton and have been reading old information for years but I still have lots of questions :smile: If I wanted to make a couple sealed with a port to inject spores into then put in sterile water later for a culture syringe is that feasible? Should I not bother and just do a liquid culture? I'm looking to do some isolation but right now I just wanna get it going. I also plan to do either a syringe to WBS or wait on the agar isolation for a WBS to already composted cow poo. I have gypsum but kinda confused when and when not to use it.  Any suggestions welcomed! Big thanks to all the cool people on here that stop and take the time to mentor others!!


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Re: Pasty Agar Tek [Re: Happy Littletree]
    #19431137 - 01/16/14 11:25 PM (10 years, 1 month ago)

I wouldn't inoculate a liquid culture with anything but an agar wedge. If you want you can inject sterile water into the plate then give it a little swirl then aspirate it back into the syringe, but inoculatiom with a wedge is prettu safe amd you can G2G from there.


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Re: Pasty Agar Tek [Re: Pastywhyte]
    #19431169 - 01/16/14 11:30 PM (10 years, 1 month ago)

and skip the composted cow poop, you want field aged stuff for proper results, also don't over think gypum, chuck handfulls in here and there. glad to see this thread has sparked some fiire for people, good shit pasty


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Re: Pasty Agar Tek [Re: cronicr]
    #19431984 - 01/17/14 05:59 AM (10 years, 1 month ago)

Quote:

cronicr said:
and skip the composted cow poop, you want field aged stuff for proper results, also don't over think gypum, chuck handfulls in here and there. glad to see this thread has sparked some fiire for people, good shit pasty




QFT on all fronts!!  (except cow poop, don't know much about it :shrug: but i'd trust cron on it)


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Re: Pasty Agar Tek [Re: blindingleaf]
    #19432996 - 01/17/14 12:10 PM (10 years, 1 month ago)

Well my lot of 96 came today. I hadn't read all the way through or noticed the part about modifying lids until just now. :facepalm:  I've always used small short 4oz mason jars with the lid upside down. I know it won't be a hassle to mod the lids but I'm gonna try a run of them slightly cracked as aside from price, space, and weight being a factor I liked the idea of being able to have these laying around when not in use and not raising any questions. And just being able to use them for their intended purposes as well as for agar. I'll be sure to have plenty on hand for intended purpose with 96 though but if I can do a successful run without altering the lids I'll go that route. At worst I waste a lil agar and possible a small percentage of the rounds. I'll be sure to share how it goes.


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Re: Pasty Agar Tek [Re: DeadPhan]
    #19433121 - 01/17/14 12:39 PM (10 years, 1 month ago)

Just had an idea.  What about taking te mini rounds filled with agar, and cracking the lid, and then placing them in another large container inside the pressure cooker.  This would not really be feasible for most, but I have an aa930. I'm still racking my brain for what. So far I've considered a really large pickle jar. Or maybe a small paint bucket or can?  I don't know.  Just a thought


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Re: Pasty Agar Tek [Re: DeadPhan]
    #19433451 - 01/17/14 02:29 PM (10 years, 1 month ago)

Or would I be just as fine if I was to place te mini rounds in another larger pp5 Tupperware with a modded lid and just keep the mini round lids closed without modding the lid on those?  Something like this maybe? 


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Edited by DeadPhan (01/17/14 02:46 PM)


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Re: Pasty Agar Tek [Re: DeadPhan]
    #19433502 - 01/17/14 02:42 PM (10 years, 1 month ago)

the only thing that might work is if u wrap them in foil really well with the lid cracked.  with this though, u rsk steam penetrating the foil and watering down ur agar.  maybe try it will 4 of them, cause u have extra haha and then see if that works.


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Re: Pasty Agar Tek [Re: blindingleaf]
    #19433534 - 01/17/14 02:49 PM (10 years, 1 month ago)

Check out the sterlite I just posted. Ya don't think if I threw them in something like that with lid closed?  Or even with lid cracked and negate the news for foil...


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Re: Pasty Agar Tek [Re: DeadPhan]
    #19433587 - 01/17/14 02:59 PM (10 years, 1 month ago)

good question.  I'm not sure.  i once tried to pasteurize in a large pp5 5 gallon bucket, but the heat took SOOOOO long to get thru I actually took it out and put the sub in jars and re did it.  now that was not under pressure, so maybe it would work with the pressure.  i know the v tek uses plastic pp5s and the claim in that because it is plastic, the that transfers more quickly, resulting in less time had for sterilizing the grain.  u could try it, but like i said, only do four so that if it does not work, its not a big loss.

im not sure how u would go about it tho.

try one cycle with that, and once cycle with cracked lid completely wrapped in foil.  honestly, the lid mod is not that bad though...just line up 16-20 on ur table, light a small piece of nail and touch the lids (one hot nail can make a hole in about 8 containers IME) and then cover with micropore/poly.


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Re: Pasty Agar Tek [Re: blindingleaf]
    #19433822 - 01/17/14 03:44 PM (10 years, 1 month ago)

Maybe I'll just try a few mini rounds like you said filled with water in the sterlite with lids closed. If that doesn't work at least i won't wast agar.  I hear ya on the lid mod not being a big deal but I'd much rather keep the lids in tact if possible.


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Re: Pasty Agar Tek [Re: DeadPhan]
    #19433859 - 01/17/14 03:51 PM (10 years, 1 month ago)

if u do it, do it with agar in the PP5's so u know it got sterilized, otherwise u may just end up with unsterilized agar when u go to try the real run


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Re: Pasty Agar Tek [Re: blindingleaf]
    #19433979 - 01/17/14 04:25 PM (10 years, 1 month ago)

If you crack the mini's and not the outside container i think it should work. If you crack both though steam is just going to make its way through all of it. Make sure the outer container is strong enough to withstand the pressure though :smile:


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Re: Pasty Agar Tek [Re: Valyr]
    #19436837 - 01/18/14 08:49 AM (10 years, 1 month ago)

thanks for the tek brother!!!

GT ISOLATE

going to be transferring to new agar this week under the new flowhood.


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Re: Pasty Agar Tek [Re: Yuri.Pono]
    #19436937 - 01/18/14 09:18 AM (10 years, 1 month ago)

:headbanger:


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Re: Pasty Agar Tek [Re: Pastywhyte]
    #19451205 - 01/21/14 02:27 AM (10 years, 1 month ago)

I know I should probably start another thread for this but since there are no results yet I thought I'd just put it here.

I inoculated 20 easy plates with ATL Galindoi spore prints (courtesy of Frank on a previous trade) I used two different methods. for 10 of them I used a inoculating loop / wire that got flamed, dragged through the agar, dragged on the print, then dragged back onto the agar. 

The other method I used was sterile Q-tips (sterile swabs)  I ripped open the swab package, swiped the print then swiped the agar.

I want to begin growing ONLY PS. Galindoi from now on.  I also have a long term goal of being able to bring my Air Pump tub ( https://www.shroomery.org/forums/showflat.php/Number/19370404 )to the table for fruiting a forest of Galindoi fruits.  Sclerotia come first of course though.

Though it's safe to say... Pasty Plates are the outline of my opus.


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Re: Pasty Agar Tek [Re: elasticaltiger]
    #19451208 - 01/21/14 02:28 AM (10 years, 1 month ago)

Shit Sorry. Also!!!!

My pasty plates use polyfill instead of SFD's or Micropore....

Because I fucking CAN... that's why...


--------------------
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Afraid to Start Growing From Your Own Prints? Drop it Like a Tiger!
No Pouring. No Syringes. No Cutting. No flaming. No Contamination. No Bullshit.

"The best thing to do while your waiting is to start more stuff. I usually got so much happening that I have tossed projects simply because I didn't have time for them. -Pastywhite QFT

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Re: Pasty Agar Tek [Re: elasticaltiger]
    #19452047 - 01/21/14 09:46 AM (10 years, 1 month ago)

Quote:

elasticaltiger said:
Shit Sorry. Also!!!!

My pasty plates use polyfill instead of SFD's or Micropore....

Because I fucking CAN... that's why...




Good stuff, cronicr would approve I'm sure :super:

Also enjoy the galindoi, stones are my favorite for sure :awesomenod:


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Re: Pasty Agar Tek [Re: Pastywhyte]
    #19452605 - 01/21/14 12:17 PM (10 years, 1 month ago)

so I'm making another round of these now, and i just re read the OP because i forgot how long pasty suggested to PC them, and i noticed pasty, u only raise them with 2 rows of rings from bottom of PC!  damn, I have been placing three 1/2 pints under my pc rack raising them that much higher thinking thats what u had done to prevent boil over.  guess i don't have to do that anymore, and can fit more in there now!


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Re: Pasty Agar Tek [Re: blindingleaf]
    #19452787 - 01/21/14 12:59 PM (10 years, 1 month ago)

I usually just raise them enough so they are out of the water. Since you are not PCing as long as you would for grains, less water is needed.


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Re: Pasty Agar Tek [Re: Pastywhyte]
    #19452844 - 01/21/14 01:10 PM (10 years, 1 month ago)

next time, will do.  man i should have pc'd these last night before i :raveface:  now i gotta wait for pc to cool and i still have shit to pasteurize


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Re: Pasty Agar Tek [Re: blindingleaf]
    #19452889 - 01/21/14 01:20 PM (10 years, 1 month ago)

Myself and others take ours out as soon as PC unlocks from pressure. I throw them in my sab but I believe others just throw them on the counter.


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Re: Pasty Agar Tek [Re: magickspore]
    #19452904 - 01/21/14 01:22 PM (10 years, 1 month ago)

you certainly can your just gonna have a litte more condensation then normal


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Re: Pasty Agar Tek [Re: cronicr]
    #19452913 - 01/21/14 01:23 PM (10 years, 1 month ago)

Hmmm never made that connection although it makes perfect sense. Well that explains my problem haha.


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Re: Pasty Agar Tek [Re: magickspore]
    #19452941 - 01/21/14 01:28 PM (10 years, 1 month ago)



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Re: Pasty Agar Tek [Re: cronicr]
    #19453102 - 01/21/14 02:04 PM (10 years, 1 month ago)

yea thats why i wait to take them out.  its hard to see in "high def" with these in the first place compared to regular plastic petris, but with extra condensation, even more so.

yo cron, u should change ur title to "a thread without the cron is like a beat without a snare"


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Re: Pasty Agar Tek [Re: blindingleaf]
    #19453356 - 01/21/14 03:16 PM (10 years, 1 month ago)

:laugh2:


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Re: Pasty Agar Tek [Re: cronicr]
    #19456280 - 01/22/14 06:05 AM (10 years, 1 month ago)

pasty and other who use this tek, thought i'd tell u what i tried tonight, chime in and either :mad2: me out cause it was dumb, or :rockon: me on cause it just may work!

so with this tek, the only consistent draw back i notice from multiple users of it, and other no pour teks like it, is the condensation.  this can be  slightly remedied by letting agar cool after pouring, before PC'ing.  this cycle, mine cooled over 9 hours before pc'ing, there was NO moisture, condensation on walls or lids of the PP5.  after pc cycle, i let it cool for over 12 hours.  cool to the touch, not even warm.  took them out, CONDENSATION everywhere WTF!!  this does not happen all the time, but this time i thought for sure because i waited before pc and after pc cool down way more than i usually do that there would be no condensation.

  so what i did, while working in the SAB doing transfers, is after i take the foil off, i use the paper towel on top of lid (also sterile at this point) and wiped the walls of the container before dropping wedges in.  obviously, if u do a cycle with a lot of these plates, u may not use ALL of them right outta the pc, so those put aside for a later date, will probably not still have a convenient sterilized paper towel to use for this (or do u think it would still b sterile, as it was wrapped in foil entire time?).

i will let u guys know if it works.  whatd'ya think?  u can call me crazy, i don't mind.  i did this once with a cotton ball (not even sterile, straight outta the bag) and a regular petri and it worked a while back (but it was one time, one plate, not conclusive enough), so i figured why not try it again with these because i use this tek a lot.


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Re: Pasty Agar Tek [Re: blindingleaf]
    #19456386 - 01/22/14 07:06 AM (10 years, 1 month ago)

Unrelated to the topic, but I saw your subtext "I use parentheses a lot" and then I started reading your post and was like "hmm, yes, I agree."  Got a good chuckle out of that sequence.


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Re: Pasty Agar Tek [Re: Inocuole]
    #19456445 - 01/22/14 07:42 AM (10 years, 1 month ago)

:blush:, i tend to have interjecting thoughts as i post, and they make more sense in () than adding another sentence.  i hope my posts make sense even with them.  of couse when i read them they do, but dunno if others are just like "WTF??  fuck that parenthetical noise!!"  haha


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Re: Pasty Agar Tek [Re: blindingleaf]
    #19456675 - 01/22/14 09:25 AM (10 years, 1 month ago)

condensation with pp5's is gonna happen, all you can do is try to limit it. i think it happens due to the way we simply shut the pc off at 45 minutes rather then slowly let the pressure drop but being a stoner i always forget to try this lol, but the condensation never takes long to go away


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Re: Pasty Agar Tek [Re: cronicr]
    #19456706 - 01/22/14 09:35 AM (10 years, 1 month ago)

Quote:

cronicr said:
condensation with pp5's is gonna happen, all you can do is try to limit it. i think it happens due to the way we simply shut the pc off at 45 minutes rather then slowly let the pressure drop but being a stoner i always forget to try this lol, but the condensation never takes long to go away




I think your right.  Lots of times my worst condensation would happen when I shut the stove off and took it of the burner. Now I drop the heat slow and leave the pc on the burner to cool off slow. I rarely have condensation issues.


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Re: Pasty Agar Tek [Re: cronicr]
    #19456751 - 01/22/14 09:48 AM (10 years, 1 month ago)

Quote:

cronicr said:
condensation with pp5's is gonna happen, all you can do is try to limit it. i think it happens due to the way we simply shut the pc off at 45 minutes rather then slowly let the pressure drop but being a stoner i always forget to try this lol, but the condensation never takes long to go away





I think:

Condensation will happen because of the temp differential that easily can exist with a thin plastic container.  No matter how slow or fast you cool your PC/Autoclave, as it cools the material inside of the jar will ALWAYS be warmer than the surrounding environment.  I feel that this is the best explanation of the condensation issue. 

I am thinking that cooling off the PC slowly may reduce the amount, but as chronicr states, it will never totally alleviate.  Another issue with this idea is that a slow cool off is still going to be HOT and cooking the agar....I don't know how this would affect overall cook time.  Do you change to 30min @ 15, and 2 hours of cooling down from 250f to room temp?  I doubt it matters, but just a thought.

Glass jars experience much less because of the glass being a HORRIBLE conductor of heat.  By the logic that glass takes longer to heat, it also will take much longer to cool versus the plastic. 

Just a theory based on the ease of conductivity of heat from outside the containers to the inside.


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Re: Pasty Agar Tek [Re: HypnotoadCroaked]
    #19456990 - 01/22/14 10:40 AM (10 years, 1 month ago)

what do all you no-pour tekkies use for culture slants when you want to save some genetics?  do you keep master cultures in no-pour agar rounds?  do you actually pour agar into slants sometimes? 

I'm thinking of using no-pour rounds as master culture jars...


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Re: Pasty Agar Tek [Re: Generic]
    #19457001 - 01/22/14 10:43 AM (10 years, 1 month ago)

I use slants for long term storage. Have not really found a substitute for slants.


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Re: Pasty Agar Tek [Re: Generic]
    #19457003 - 01/22/14 10:43 AM (10 years, 1 month ago)

Quote:

Generic said:
what do all you no-pour tekkies use for culture slants when you want to save some genetics?  do you keep master cultures in no-pour agar rounds?  do you actually pour agar into slants sometimes? 

I'm thinking of using no-pour rounds as master culture jars...




Master slants are already no pour, just PC the culture tubes with agar in them.  Take them out before totally cooled and put them on a slant to finish cooling/solidify.


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Re: Pasty Agar Tek [Re: Forrester]
    #19458172 - 01/22/14 03:37 PM (10 years, 1 month ago)

Quote:

I think your right.  Lots of times my worst condensation would happen when I shut the stove off and took it of the burner. Now I drop the heat slow and leave the pc on the burner to cool off slow. I rarely have condensation issues.




ok will try this next time.

no thoughts on the paper towel?


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Re: Pasty Agar Tek [Re: Pastywhyte]
    #19460645 - 01/23/14 01:07 AM (10 years, 1 month ago)

Quote:

Pastywhyte said:
Have not really found a substitute for slants.




There isn't one :shrug:


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Re: Pasty Agar Tek [Re: FrankHorrigan]
    #19463406 - 01/23/14 04:30 PM (10 years, 1 month ago)

http://www.shroomery.org/8509/Resurrecting-a-Better-Method-for-Long-Term-Storage-of-Mushroom-Cultures

Does this method not work for y'all?  Reading about and trying it myself, i like this idea better because it seems like it'll last much longer and requires no refrigeration.  Already have a couple vials from a multispore plate just to explore the process.  The jars i have growing out from them are nearly 100%  Anyway, anyone else use this method to store their strains?


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Re: Pasty Agar Tek [Re: J. Jack Flash]
    #19463482 - 01/23/14 04:49 PM (10 years, 1 month ago)

jm mcdoogle (i think that his poster name) posded a thread about that little bit back and many responded, some who have tried with success if i remember correctly. look at his ratings and someone links to it, i think amanita virosa


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Re: Pasty Agar Tek [Re: blindingleaf]
    #19463500 - 01/23/14 04:52 PM (10 years, 1 month ago)

no contam yet on my "sterile paper towel" experiment.


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Re: Pasty Agar Tek [Re: J. Jack Flash]
    #19467000 - 01/24/14 10:54 AM (10 years, 1 month ago)

Quote:

J. Jack Flash said:
http://www.shroomery.org/8509/Resurrecting-a-Better-Method-for-Long-Term-Storage-of-Mushroom-Cultures

Does this method not work for y'all?  Reading about and trying it myself, i like this idea better because it seems like it'll last much longer and requires no refrigeration.  Already have a couple vials from a multispore plate just to explore the process.  The jars i have growing out from them are nearly 100%  Anyway, anyone else use this method to store their strains?




I gave that a cursory glance, are you asking if you can use water to store cultures? I think that would be called a grain LC :shrug:


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InvisibleInocuole
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Re: Pasty Agar Tek [Re: FrankHorrigan]
    #19468269 - 01/24/14 03:51 PM (10 years, 1 month ago)

"Myceliated water" is what RR likes to refer to it as, and I must respectfully agree, since they're not living on the water so much as hanging in stasis in it.  I'm not sure however if that's any more likely to survive over a length of time VS tradtional LC, which is the only place I could see a downside in that method.  There is that thread floating around about a year old "glc" being resurrected, so who knows, maybe I should've kept some of my old ones.


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Re: Pasty Agar Tek [Re: Inocuole] * 1
    #19468999 - 01/24/14 06:54 PM (10 years, 1 month ago)

i think there's a distinction between that and liquid culture since there's zero nutrition in the liquid medium, and grain never enters into it.  like you said, in stasis.  i looked for the thread you mentioned, leaf, but i couldn't find.  i'll try again later on.
(pasty, i feel it's marginally on topic, but if you disagree, please advise)
what i did probably sounds dumb, but i like it and so far it has been successful.  using dropper dram vials such as these, i filled them with distilled water.  PC'd for some amount to time, i forget off hand, to sterilize them.  took two of them, and a fluffy multispore plate.  one at a time in still air, squirted a few droppers full of water into the plate, scraped around with the dropper and slurped it all back up.  rinsed, repeated.  based on the work cited in the article, it should/could/would (might) remain viable for years, if stored in reasonable conditions.  then just put a drop on agar to grow out for master inoculation and bickity bam, the motherfucker is rubble.  (i love that 'motherfucker' wasn't marked as a misspelling)  one drop at time, should last for at least as long as i'll be into growing, which i feel will be quite long, indeed.  and when i'm done, they'll make great gifts.

of course, it's not worth the effort for the sake of multispore, but with isolates, i feel it shows real promise, without refrigeration, or worries about repeating the slant process every year or so.

in retrospect, yeah this is a clear :jacked:, but i'm not wasting the above typing... sorry, y'all.  you would not believe what a geek i am.


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Re: Pasty Agar Tek [Re: J. Jack Flash]
    #19469046 - 01/24/14 07:02 PM (10 years, 1 month ago)

interesting jj, def let us know if it works (in another thread).  I'm also down to try!


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Re: Pasty Agar Tek [Re: FrankHorrigan]
    #19469426 - 01/24/14 08:35 PM (10 years, 1 month ago)

I don't know if anyone else has mentioned this yet, but I love how you can just slide the whole puddle out into your grain jar instead of messing around with cutting (unless you want to of course.)

It makes for really easy grain inoculation.  Sure you could get four jars with one but it's so darn easy to just drop the whole thing in and call it good.  The size of the puddle is basically the same as a 'third' of a regular petri. :awesome:


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Re: Pasty Agar Tek [Re: elasticaltiger]
    #19469484 - 01/24/14 08:47 PM (10 years, 1 month ago)

Shit man I never tried that. Gonna give it a shot :rockon:


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Re: Pasty Agar Tek [Re: elasticaltiger]
    #19469514 - 01/24/14 08:54 PM (10 years, 1 month ago)

Quote:

elasticaltiger said:
I don't know if anyone else has mentioned this yet, but I love how you can just slide the whole puddle out into your grain jar instead of messing around with cutting (unless you want to of course.)

It makes for really easy grain inoculation.  Sure you could get four jars with one but it's so darn easy to just drop the whole thing in and call it good.  The size of the puddle is basically the same as a 'third' of a regular petri. :awesome:





It's so dry here, mine crack into pieces within a day after cutting my first wedge anyway.  So there's a bunch of wedges, handy!


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Re: Pasty Agar Tek [Re: Forrester]
    #19470171 - 01/25/14 12:22 AM (10 years, 1 month ago)

Quote:

*** About six milliliters of sterile distilled water is pipetted aseptically into a freshly growing culture. The fragments of hyphae are dislodged by lightly scraping the aerial growth with the same pipette, and the resulting suspension is withdrawn and transferred to a sterile glass vial. Put plenty of inoculum into each vial to insure success.




Sounds like they were knocking mycelium of the "freshly growing culture"  that was perhaps on grains (knocked into water) and transferring them to a sterile glass vial :shrug:


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Re: Pasty Agar Tek [Re: FrankHorrigan]
    #19479785 - 01/27/14 12:56 AM (10 years, 29 days ago)

If you're going to dump the whole plate into the jar I would suggest these things:

1) Make sure full colonization is pretty much complete (wait a day after full colonization just in case.)

2) squeeze the bottom of the containers before you open them. This will cause the puddle to 'jump' out of it's wedge a little. From there you can just shake it around and drop it.


--------------------
First time growing cakes? DON'T make a Shotgun Fruiting Chamber

The Shmuvbox. - The Old TC's Like it :shrug:

Afraid to Start Growing From Your Own Prints? Drop it Like a Tiger!
No Pouring. No Syringes. No Cutting. No flaming. No Contamination. No Bullshit.

"The best thing to do while your waiting is to start more stuff. I usually got so much happening that I have tossed projects simply because I didn't have time for them. -Pastywhite QFT

Pastywhite's Easy Agar Tek (PastyPlates)

Tiger Drop Video Demos By munchauzen

Van Gogh would’ve sold more than one painting if he’d put tigers in them.―Bill Watterson

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Re: Pasty Agar Tek [Re: elasticaltiger]
    #19480119 - 01/27/14 05:27 AM (10 years, 29 days ago)

yea i def gotta try that.  its strange to hear "full colonization" in relation to petris.


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Re: Pasty Agar Tek [Re: blindingleaf]
    #19480603 - 01/27/14 10:03 AM (10 years, 28 days ago)

Quote:

blindingleaf said:
yea i def gotta try that.  its strange to hear "full colonization" in relation to petris.




That's because it's poor practice to let it crawl all the way to the outer edges of the dish, that is where contaminants tend to slip in, if they are going to.


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You should take a look. :hehehe:


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Re: Pasty Agar Tek [Re: FrankHorrigan]
    #19480830 - 01/27/14 11:19 AM (10 years, 28 days ago)

Hey FrankHorrigan whats with the block Anne H. in your signature?  Whats that all about?


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Re: Pasty Agar Tek [Re: Delection]
    #19481192 - 01/27/14 12:48 PM (10 years, 28 days ago)

Quote:

Delection said:
Hey FrankHorrigan whats with the block Anne H. in your signature?  Whats that all about?



:whathesaid:

I could UTFSE but I'm betting you have some stunning examples off the top of your head.


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