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Inocuole
Scalpel of Evil's Bane



Registered: 11/21/11
Posts: 24,863
Loc: ★
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My APE syringe was pretty nasty too. May as well keep transferring away from bacteria. At least you know you've got something that'll put up a fight against minor nasties.
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MudaFuka
Poppin bottles



Registered: 12/14/13
Posts: 18,648
Loc: Canada
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Re: Pasty Agar Tek [Re: Inocuole]
#20995327 - 12/18/14 05:33 PM (9 years, 1 month ago) |
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I got a contaminated PE syringe from one of the most trusted sponsors. Shit just happens some times. The bacteria grew really fast on agar but the spores germinated on grain enough that I managed to put a colonised grain on agar to salvage it. I have gotten some really good grows from that shitty syringe so I never bothered to contact the vender.
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taGyo
Strainiac/AMU



Registered: 10/16/14
Posts: 18,802
Loc: Journal Land
Last seen: 5 years, 3 months
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Re: Pasty Agar Tek [Re: MudaFuka]
#20995341 - 12/18/14 05:35 PM (9 years, 1 month ago) |
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Yeah I knocked four jars up with the APE and two of them are showing the tiniest smidgen of growth. Gonna wait for it to progress a little bit more and then take the colonized grain over to agar and work from there.
-------------------- Gyo's Better Grows TNF Q&A AMU Q&A Dominus fortunae meae sum
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elasticaltiger
Like Tigers in Coitus




Registered: 06/24/13
Posts: 8,059
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Quote:
Elf_on_a_Log said: I leave the foil on them until I have them in my SAB ready to use and don't even worry about the condensation anymore...
This guy's gonna go far.
-------------------- First time growing cakes? DON'T make a Shotgun Fruiting Chamber The Shmuvbox. - The Old TC's Like it Afraid to Start Growing From Your Own Prints? Drop it Like a Tiger! No Pouring. No Syringes. No Cutting. No flaming. No Contamination. No Bullshit. "The best thing to do while your waiting is to start more stuff. I usually got so much happening that I have tossed projects simply because I didn't have time for them. -Pastywhite QFT Pastywhite's Easy Agar Tek (PastyPlates) Tiger Drop Video Demos By munchauzen Van Gogh would’ve sold more than one painting if he’d put tigers in them.―Bill Watterson EZEKIEL 23:20
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taGyo
Strainiac/AMU



Registered: 10/16/14
Posts: 18,802
Loc: Journal Land
Last seen: 5 years, 3 months
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That's part of the pasty tek.
Btw:
Informative.
-------------------- Gyo's Better Grows TNF Q&A AMU Q&A Dominus fortunae meae sum
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Pasty Agar Tek [Re: taGyo]
#20995442 - 12/18/14 06:01 PM (9 years, 1 month ago) |
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if you can't figure out parafilm on your own after playing with it for 3 seconds then agar isn't for you in the first place
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taGyo
Strainiac/AMU



Registered: 10/16/14
Posts: 18,802
Loc: Journal Land
Last seen: 5 years, 3 months
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Shhh Bod,
Don't make me feel bad
-------------------- Gyo's Better Grows TNF Q&A AMU Q&A Dominus fortunae meae sum
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Pasty Agar Tek [Re: taGyo]
#20995699 - 12/18/14 06:44 PM (9 years, 1 month ago) |
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haha, then again the first time I wrapped dishes with saran I wrapped the whole thing not just the edge. I could see how one might not know how to cut parafilm into the strips
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taGyo
Strainiac/AMU



Registered: 10/16/14
Posts: 18,802
Loc: Journal Land
Last seen: 5 years, 3 months
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The strip size was what I was looking at tbh ,
I cut my parafilm straight down the middle and feel like an idiot now. An easy fix but damn.
-------------------- Gyo's Better Grows TNF Q&A AMU Q&A Dominus fortunae meae sum
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elasticaltiger
Like Tigers in Coitus




Registered: 06/24/13
Posts: 8,059
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Quote:
bodhisatta said: if you can't figure out parafilm on your own after playing with it for 3 seconds then agar isn't for you in the first place 
Quote:
bodhisatta said: haha, then again the first time I wrapped dishes with saran I wrapped the whole thing not just the edge. I could see how one might not know how to cut parafilm into the strips
Yeah but with saran wrap it's different because we are inherently conditioned while growing up to think of saran wrap as something to be used to engulf especially if you've worked in food service.
-------------------- First time growing cakes? DON'T make a Shotgun Fruiting Chamber The Shmuvbox. - The Old TC's Like it Afraid to Start Growing From Your Own Prints? Drop it Like a Tiger! No Pouring. No Syringes. No Cutting. No flaming. No Contamination. No Bullshit. "The best thing to do while your waiting is to start more stuff. I usually got so much happening that I have tossed projects simply because I didn't have time for them. -Pastywhite QFT Pastywhite's Easy Agar Tek (PastyPlates) Tiger Drop Video Demos By munchauzen Van Gogh would’ve sold more than one painting if he’d put tigers in them.―Bill Watterson EZEKIEL 23:20
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elasticaltiger
Like Tigers in Coitus




Registered: 06/24/13
Posts: 8,059
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Quote:
elasticaltiger said: especially if you've worked in food service.
Which most college-drop-out-mushroom-growing-hippie-stoners have... at one point or another.
-------------------- First time growing cakes? DON'T make a Shotgun Fruiting Chamber The Shmuvbox. - The Old TC's Like it Afraid to Start Growing From Your Own Prints? Drop it Like a Tiger! No Pouring. No Syringes. No Cutting. No flaming. No Contamination. No Bullshit. "The best thing to do while your waiting is to start more stuff. I usually got so much happening that I have tossed projects simply because I didn't have time for them. -Pastywhite QFT Pastywhite's Easy Agar Tek (PastyPlates) Tiger Drop Video Demos By munchauzen Van Gogh would’ve sold more than one painting if he’d put tigers in them.―Bill Watterson EZEKIEL 23:20
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Imperfect Iam
^means imperfect,not I'm perfect



Registered: 03/05/13
Posts: 7,237
Loc: center of the universe
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You can do something similar if you have a syringe and a petri of mycellium. Just spray water that has been sterilized(I would think pc it at the same time you are pc'ing some agar, and I do beleive that would be sufficient time to sterilize the water, but that's just a guess).
Let it cool, then inside your SAB, draw up some water into a syringe, and spray it over a colonized petri, then rub the mycellium off the agar(with syringe, making the mycellium suspended into the water) and draw it up into syringe, bam, li without blender!
And Stare, from what I can see, things are looking good, I would have figured you had already knocked up some grains by now?
-------------------- All you touch, and all you see, is all your life will ever be- Pink Floyd Life is what happens while you're busy making other plans- John Lennon
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taGyo
Strainiac/AMU



Registered: 10/16/14
Posts: 18,802
Loc: Journal Land
Last seen: 5 years, 3 months
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Quote:
elasticaltiger said:
Quote:
elasticaltiger said: especially if you've worked in food service.
Which most college-drop-out-mushroom-growing-hippie-stoners have... at one point or another.
QFT
-------------------- Gyo's Better Grows TNF Q&A AMU Q&A Dominus fortunae meae sum
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MudaFuka
Poppin bottles



Registered: 12/14/13
Posts: 18,648
Loc: Canada
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Re: Pasty Agar Tek [Re: taGyo]
#20996076 - 12/18/14 08:00 PM (9 years, 1 month ago) |
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I'm certainly no hippy stoner but I am a university drop out mushroom grower and I have had tons of shitty food service jobs.
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Imperfect Iam
^means imperfect,not I'm perfect



Registered: 03/05/13
Posts: 7,237
Loc: center of the universe
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Quote:
Imperfect Iam said: You can do something similar if you have a syringe and a petri of mycellium. Just spray water that has been sterilized(I would think pc it at the same time you are pc'ing some agar, and I do beleive that would be sufficient time to sterilize the water, but that's just a guess).
Let it cool, then inside your SAB, draw up some water into a syringe, and spray it over a colonized petri, then rub the mycellium off the agar(with syringe, making the mycellium suspended into the water) and draw it up into syringe, bam, li without blender!
And Stare, from what I can see, things are looking good, I would have figured you had already knocked up some grains by now?
Damn, I just tried to give an answer to questions I don't know how many pages back, LOL!
-------------------- All you touch, and all you see, is all your life will ever be- Pink Floyd Life is what happens while you're busy making other plans- John Lennon
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FrankHorrigan
The Inquisition



Registered: 01/04/11
Posts: 10,573
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Re: Pasty Agar Tek [Re: MudaFuka]
#20996146 - 12/18/14 08:11 PM (9 years, 1 month ago) |
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Quote:
MudaFuka said: I'm certainly no hippy stoner but I am a university drop out mushroom grower and I have had tons of shitty food service jobs.
Ah, food service. I prefer bartending to serving or cooking
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taGyo
Strainiac/AMU



Registered: 10/16/14
Posts: 18,802
Loc: Journal Land
Last seen: 5 years, 3 months
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Quote:
FrankHorrigan said:
Quote:
MudaFuka said: I'm certainly no hippy stoner but I am a university drop out mushroom grower and I have had tons of shitty food service jobs.
Ah, food service. I prefer bartending to serving or cooking 
More money in bartending too.
Bullshit a bunch of people, smile and wave and get tips.
-------------------- Gyo's Better Grows TNF Q&A AMU Q&A Dominus fortunae meae sum
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MudaFuka
Poppin bottles



Registered: 12/14/13
Posts: 18,648
Loc: Canada
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Re: Pasty Agar Tek [Re: taGyo]
#20996175 - 12/18/14 08:17 PM (9 years, 1 month ago) |
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I did the bar tending thing for a while when I was 18 or 19. It was better than working with food but I hate with the service industry in general. I build houses these days.
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bodhisatta 
Smurf real estate agent


Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: Pasty Agar Tek [Re: MudaFuka]
#20996179 - 12/18/14 08:18 PM (9 years, 1 month ago) |
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tour guides get mega good tips.
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Elf_on_a_Log
Elf

Registered: 07/13/14
Posts: 610
Loc: On a log
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Quote:
elasticaltiger said:
Quote:
Elf_on_a_Log said: I leave the foil on them until I have them in my SAB ready to use and don't even worry about the condensation anymore...
This guy's gonna go far.
Lol my bad, that is part of the tek isn't it. I've got my procedures so customized and ingrained into memory now that I'd forgotten what's actually written in the tek.
-------------------- "I was strolling through the woods one day when I came upon an elf sitting on a log. He offered me a strange-looking mushroom to eat. As soon as I ate it, I realized that I was the elf on the log and that my human life had been but a dream." - Elf_on_a_Log
LAGM 2.022 Grow Log
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