Home | Community | Message Board

Original Seeds Store
This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Mushroom-Hut Liquid Cultures   Left Coast Kratom Buy Kratom Capsules   North Spore Injection Grain Bag   PhytoExtractum Kratom Powder for Sale

Jump to first unread post Pages: < First | < Back | 88 | 89 | 90 | 91 | 92 | 93 | 94 | 95 | 96 | 97 | 98 | 99 | 100 | 101 | 102 | 103 | 104 | 105 | 106 | 107 | 108 | Next > | Last >
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
OfflineBumbaclotjohnson
Sequoia Farmer
Male User Gallery


Registered: 01/12/14
Posts: 1,067
Loc: USA Flag
Last seen: 3 years, 9 months
Re: Pasty Agar Tek [Re: NumeroEno]
    #20990245 - 12/17/14 04:22 PM (9 years, 1 month ago)

Has anyone considered making their pastey plates with plastic lids 1/4 pint jars and sfds?


--------------------
FREE PRINTS

I lie a lot on the internet.
 
             
                                                                                            :mushroom2:    trade list      :mushroom2:
                                          :noargument:                                                                            :god2:


Extras: Filter Print Post Top
InvisibleInocuole
Scalpel of Evil's Bane
 User Gallery

Folding@home Statistics
Registered: 11/21/11
Posts: 24,863
Loc: ★
Trusted Cultivator
Re: Pasty Agar Tek [Re: Bumbaclotjohnson]
    #20990354 - 12/17/14 04:44 PM (9 years, 1 month ago)

Quote:

Bumbaclotjohnson said:
Has anyone considered making their pastey plates with plastic lids 1/4 pint jars and sfds?





:facepalm:

People did this before pasty plates, this tek is kind of an upgrade.


--------------------
                            :rainbowdrink: Tea doesn't work?                            AMU  (Q & A)                  Grain prep for Intergalactic Space Oats :pes:     

Coir-ca-cola
Jokes are funny until they're about you


Extras: Filter Print Post Top
InvisiblePsillyDoctor
Strangers

Registered: 10/23/14
Posts: 174
Re: Pasty Agar Tek [Re: Inocuole]
    #20992868 - 12/18/14 06:16 AM (9 years, 1 month ago)

So I got a question for you guys. I did this tek for the plates and used polyfil as I do not have any micropore tape. I used a syringe and and let the plate cool to room temp. then put my foil over the tops and pc'd them for 45@15psi. then let them cool to room temp today. I have condensation on the walls. do you guys keep the foil on until you use them or would it be ok to pull them off to help dry off the condensation? i think i read it will clear up in a few days. just didn't know how you guys handled it.


--------------------


Extras: Filter Print Post Top
OfflineBumbaclotjohnson
Sequoia Farmer
Male User Gallery


Registered: 01/12/14
Posts: 1,067
Loc: USA Flag
Last seen: 3 years, 9 months
Re: Pasty Agar Tek [Re: PsillyDoctor]
    #20992877 - 12/18/14 06:22 AM (9 years, 1 month ago)

Condensation is ok, you let your pressure fluctuate too fast while cooking. Bring the pressure up slow and down slow, the problem will go away. I dunno what this talk of a syringe is about though.


--------------------
FREE PRINTS

I lie a lot on the internet.
 
             
                                                                                            :mushroom2:    trade list      :mushroom2:
                                          :noargument:                                                                            :god2:


Extras: Filter Print Post Top
InvisibleElf_on_a_Log
Elf

Registered: 07/13/14
Posts: 610
Loc: On a log
Re: Pasty Agar Tek [Re: Bumbaclotjohnson]
    #20994323 - 12/18/14 02:05 PM (9 years, 1 month ago)

I leave the foil on them until I have them in my SAB ready to use and don't even worry about the condensation anymore.  I've tried every tip and trick suggested in this thread and never was able to get them to come out without condensation.  It doesn't hurt anything.  When you go to inoculate them remove the foil and over the course of the next week-ish (sometimes longer) the condensation will slowly go away.  Some people like to wipe it off with the paper towel when they open the lid to inoculate, but I've found that to be an un-needed contam vector.

If you want to get a good look at what's going on in the plate while condensation is all over the walls preventing you from seeing in, hold the plate up near a ceiling light and look in through the bottom.  I've spotted bacteria that way that I couldn't see from the sides/top even without condensation on the walls, so I personally think that's the best way to look at what's going on in them anyway.


--------------------
"I was strolling through the woods one day when I came upon an elf sitting on a log.  He offered me a strange-looking mushroom to eat.  As soon as I ate it, I realized that I was the elf on the log and that my human life had been but a dream."
- Elf_on_a_Log



LAGM 2.022 Grow Log


Extras: Filter Print Post Top
Invisiblestareatclouds
star eat clouds?
 Unread Journal User Gallery


Registered: 09/29/14
Posts: 9,887
Trusted Cultivator
Re: Pasty Agar Tek [Re: Elf_on_a_Log]
    #20994370 - 12/18/14 02:17 PM (9 years, 1 month ago)

I just wipe it off with the sterilized paper towel in my SAB when I go to use them. It also sounds like he inoculated and then PC'd the spores to death with the way he wrote it, heh.


Extras: Filter Print Post Top
OfflinetaGyo
Strainiac/AMU
Male User Gallery


Registered: 10/16/14
Posts: 18,802
Loc: Journal Land Flag
Last seen: 5 years, 3 months
Re: Pasty Agar Tek [Re: stareatclouds]
    #20994378 - 12/18/14 02:18 PM (9 years, 1 month ago)

Elf do you use food coloring or clear agar?


--------------------
Gyo's Better Grows
TNF Q&A :rockon:
AMU Q&A

Dominus fortunae meae sum


Extras: Filter Print Post Top
Invisibleinsanemike

Registered: 02/23/14
Posts: 4,272
Re: Pasty Agar Tek [Re: stareatclouds]
    #20994392 - 12/18/14 02:21 PM (9 years, 1 month ago)

Quote:

stareatclouds said:
I just wipe it off with the sterilized paper towel in my SAB when I go to use them. It also sounds like he inoculated and then PC'd the spores to death with the way he wrote it, heh.




Sterilized paper towels?


Extras: Filter Print Post Top
InvisibleInocuole
Scalpel of Evil's Bane
 User Gallery

Folding@home Statistics
Registered: 11/21/11
Posts: 24,863
Loc: ★
Trusted Cultivator
Re: Pasty Agar Tek [Re: insanemike]
    #20994398 - 12/18/14 02:22 PM (9 years, 1 month ago)

Lol right?


--------------------
                            :rainbowdrink: Tea doesn't work?                            AMU  (Q & A)                  Grain prep for Intergalactic Space Oats :pes:     

Coir-ca-cola
Jokes are funny until they're about you


Extras: Filter Print Post Top
InvisibleElf_on_a_Log
Elf

Registered: 07/13/14
Posts: 610
Loc: On a log
Re: Pasty Agar Tek [Re: taGyo]
    #20994414 - 12/18/14 02:26 PM (9 years, 1 month ago)

Quote:

taGyo said:
Elf do you use food coloring or clear agar?



I use blue food coloring.  It's a fairly deep blue but I can still see through it well.

Actually I've got a pic that shows the color...

You can see it fairly well on the plates on the upper shelf.


--------------------
"I was strolling through the woods one day when I came upon an elf sitting on a log.  He offered me a strange-looking mushroom to eat.  As soon as I ate it, I realized that I was the elf on the log and that my human life had been but a dream."
- Elf_on_a_Log



LAGM 2.022 Grow Log


Extras: Filter Print Post Top
OfflinetaGyo
Strainiac/AMU
Male User Gallery


Registered: 10/16/14
Posts: 18,802
Loc: Journal Land Flag
Last seen: 5 years, 3 months
Re: Pasty Agar Tek [Re: Elf_on_a_Log]
    #20994434 - 12/18/14 02:29 PM (9 years, 1 month ago)

I use the same blue,
Mine is a little darker.

:thumbup:

I'm gonna get some pics of something in one of my jars I think is bacteria. Hopefully someone here can confirm/deny. I reached my weekly upload limit so I'm gonna upload it to paste image.


--------------------
Gyo's Better Grows
TNF Q&A :rockon:
AMU Q&A

Dominus fortunae meae sum


Extras: Filter Print Post Top
OfflinetaGyo
Strainiac/AMU
Male User Gallery


Registered: 10/16/14
Posts: 18,802
Loc: Journal Land Flag
Last seen: 5 years, 3 months
Re: Pasty Agar Tek [Re: taGyo]
    #20994486 - 12/18/14 02:39 PM (9 years, 1 month ago)

This is bacteria, correct? I'm just wondering because there's no fucking way all 8 APE syringes have bacteria. I've had it before and I KNOW what bacteria looks like but I just need a second fucking opinion so I know I'm not losing my mind.

http://s4.postimg.org/ey9ji4lul/WP_20141218_15_55_18_Pro_1.jpg

The other ones seem to have wet spots on them if you know what I mean? Water kind of slightly poking up above the agar.


Those are myc dots and a little of the water I was talking about:

http://s2.postimg.org/7paqm9fgp/WP_20141218_16_32_57_Pro_1.jpg

This is the only APE I've got to germinate, should I transfer this:

http://s4.postimg.org/v6hi2vdst/WP_20141218_16_34_01_Pro_1.jpg

Tissue sample from a cake on it's second transfer, look at that wicked bacteria:

http://s9.postimg.org/y3cxk906n/WP_20141218_16_34_18_Pro_1.jpg

Once again I reached my weekly upload limit so excuse the external links.


--------------------
Gyo's Better Grows
TNF Q&A :rockon:
AMU Q&A

Dominus fortunae meae sum


Edited by taGyo (12/18/14 02:39 PM)


Extras: Filter Print Post Top
InvisibleElf_on_a_Log
Elf

Registered: 07/13/14
Posts: 610
Loc: On a log
Re: Pasty Agar Tek [Re: taGyo]
    #20994522 - 12/18/14 02:46 PM (9 years, 1 month ago)

Yeah you should transfer that APE.  Make a few transfers from it from on the left side of the 4 you've got written on it.  Try taking tiny wedges and also try scraping some of the myc off the top to hopefully avoid the bacteria underneath.  You should easily be able to get a clean culture out of that with just a transfer or two.


--------------------
"I was strolling through the woods one day when I came upon an elf sitting on a log.  He offered me a strange-looking mushroom to eat.  As soon as I ate it, I realized that I was the elf on the log and that my human life had been but a dream."
- Elf_on_a_Log



LAGM 2.022 Grow Log


Extras: Filter Print Post Top
OfflinetaGyo
Strainiac/AMU
Male User Gallery


Registered: 10/16/14
Posts: 18,802
Loc: Journal Land Flag
Last seen: 5 years, 3 months
Re: Pasty Agar Tek [Re: Elf_on_a_Log]
    #20994529 - 12/18/14 02:47 PM (9 years, 1 month ago)

That's what I was thinking.

I just hope it's not a shitty culture. Really pissed I have 8 syringes and even some of the new ones are showing straight bacteria.


--------------------
Gyo's Better Grows
TNF Q&A :rockon:
AMU Q&A

Dominus fortunae meae sum


Extras: Filter Print Post Top
InvisibleFrankHorrigan
The Inquisition
Male


Registered: 01/04/11
Posts: 10,573
Trusted Cultivator
Re: Pasty Agar Tek [Re: taGyo]
    #20994556 - 12/18/14 02:51 PM (9 years, 1 month ago)

This is why you should always get syringes from our sponsors, or be very careful and make your own :wink:

If I'm trading with someone, I ask for a print. I've never gotten a syringe from another member that hasn't been gross with contams.

Obviously you can trust more experienced members but my golden rule for trading is prints for prints.


--------------------

Yes, you can bump my old threads with a question.
Here is how I get things done.
You should take a look. :hehehe:


Frank's tips and tricks. Updated on 3/21/14
AMU- Get an answer here -AMU


Extras: Filter Print Post Top
OfflinetaGyo
Strainiac/AMU
Male User Gallery


Registered: 10/16/14
Posts: 18,802
Loc: Journal Land Flag
Last seen: 5 years, 3 months
Re: Pasty Agar Tek [Re: FrankHorrigan]
    #20994560 - 12/18/14 02:52 PM (9 years, 1 month ago)

All bought from our trusted vendors :lol:,

I don't trust anyone :wink:

EDIT:

I bought four originally from a sponsor and got nothing but bacteria on all plates except the APE 4.1 which is my second drop of APE from another syringe. Funny thing is the first drop contaminated. I contacted the vendor and said I couldn't identify anything but bacteria and they sent me four more.

Two of them are already showing bacteria. Guess I'll just clean this one up. Was hoping for some stronger growth though.


--------------------
Gyo's Better Grows
TNF Q&A :rockon:
AMU Q&A

Dominus fortunae meae sum


Edited by taGyo (12/18/14 02:53 PM)


Extras: Filter Print Post Top
InvisibleFrankHorrigan
The Inquisition
Male


Registered: 01/04/11
Posts: 10,573
Trusted Cultivator
Re: Pasty Agar Tek [Re: taGyo]
    #20994581 - 12/18/14 02:54 PM (9 years, 1 month ago)

You should have told the sponsor that you saw bacteria under the scope and requested clean syringes. Our sponsors are usually great about that.


--------------------

Yes, you can bump my old threads with a question.
Here is how I get things done.
You should take a look. :hehehe:


Frank's tips and tricks. Updated on 3/21/14
AMU- Get an answer here -AMU


Extras: Filter Print Post Top
OfflinetaGyo
Strainiac/AMU
Male User Gallery


Registered: 10/16/14
Posts: 18,802
Loc: Journal Land Flag
Last seen: 5 years, 3 months
Re: Pasty Agar Tek [Re: FrankHorrigan]
    #20994586 - 12/18/14 02:55 PM (9 years, 1 month ago)

I did, check the edit :lol:

Not my first rodeo :wink:


--------------------
Gyo's Better Grows
TNF Q&A :rockon:
AMU Q&A

Dominus fortunae meae sum


Extras: Filter Print Post Top
InvisibleFrankHorrigan
The Inquisition
Male


Registered: 01/04/11
Posts: 10,573
Trusted Cultivator
Re: Pasty Agar Tek [Re: taGyo]
    #20994602 - 12/18/14 02:57 PM (9 years, 1 month ago)

I missed the edit :facepalm:

:highfive:


--------------------

Yes, you can bump my old threads with a question.
Here is how I get things done.
You should take a look. :hehehe:


Frank's tips and tricks. Updated on 3/21/14
AMU- Get an answer here -AMU


Extras: Filter Print Post Top
OfflinetaGyo
Strainiac/AMU
Male User Gallery


Registered: 10/16/14
Posts: 18,802
Loc: Journal Land Flag
Last seen: 5 years, 3 months
Re: Pasty Agar Tek [Re: FrankHorrigan]
    #20994608 - 12/18/14 02:58 PM (9 years, 1 month ago)

I'll get some big white dicks goin' eventually.

Thanks Frank,
Also your AA+ are fucking gorgeous bro. I just bought an MS syringe from a sponsor. I had heard they were small fruits but god damn. Gonna try your limited MS trick as well.

Thanks for all your teks,
:headbanger:,

- Gyo


--------------------
Gyo's Better Grows
TNF Q&A :rockon:
AMU Q&A

Dominus fortunae meae sum


Extras: Filter Print Post Top
Jump to top Pages: < First | < Back | 88 | 89 | 90 | 91 | 92 | 93 | 94 | 95 | 96 | 97 | 98 | 99 | 100 | 101 | 102 | 103 | 104 | 105 | 106 | 107 | 108 | Next > | Last >

Shop: Mushroom-Hut Liquid Cultures   Left Coast Kratom Buy Kratom Capsules   North Spore Injection Grain Bag   PhytoExtractum Kratom Powder for Sale


Similar ThreadsPosterViewsRepliesLast post
* ?NO,AGAR TEK AT SHROOMERY?? bluelou 5,732 8 12/13/19 06:16 PM
by mushboy
* Agar Tek Aristar 5,815 12 08/01/03 06:42 PM
by shirley knott
* Easy syringe tek? pleasesmokemarijuana 2,357 8 04/02/03 07:09 PM
by Roadkill
* Easy Poo Tek Audi0 3,429 8 12/15/02 09:04 AM
by PDU
* Re: Peroxidated Agar Tek sparks 4,555 6 05/08/00 12:43 AM
by Anonymous
* easy agar source? KafkaPie 1,092 1 05/13/03 11:43 PM
by micro
* Easy Syringe Tek? Tenchi 2,789 18 01/28/03 05:06 AM
by Azmodeus
* agar tek? the man 2,773 4 12/16/02 10:45 AM
by the man

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
794,008 topic views. 39 members, 237 guests and 30 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.035 seconds spending 0.012 seconds on 15 queries.